Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Demir, Selim(12)
- Turan, Ibrahim(11)
- Aliyazicioglu, Yuksel(8)
- Topal, Meryem(7)
- Şengül, Mustafa(6)
- Baltaci, Cemalettin(5)
- Topal, Fevzi(5)
- Öz, Mehmet(5)
- Ürkek, Bayram(5)
- Akar, Zeynep(4)
- Alwasel, Saleh H.(4)
- Durmaz, Lokman(4)
- Gulcin, Ilhami(4)
- Gülçin, Ilhami(4)
- Akmeşe, Osman(3)
- Bingol, Zeynebe(3)
- Gören, Ahmet C.(3)
- Karatas, Seyda Merve(3)
- Misir, Sema(3)
- Turan, İbrahim(3)
- Yapıcı, İsmail(3)
- Yildiz, Hilal(3)
- Aliyazicioglu, Yüksel(2)
- Altay, Kadriye(2)
- Baltacı, Cemalettin(2)
- Deger, Orhan(2)
- Fidan, Muhammed Said(2)
- Gocer, Hulya(2)
- Gül, Abdulkadir(2)
- Gültekin, Ergün(2)
- Gülçin, İlhami(2)
- Kalin, Pinar(2)
- Kiziltas, Hatice(2)
- Kose, Leyla Polat(2)
- Küçük, Murat(2)
- Okan, Onur Tolga(2)
- Sarikaya, S. Beyza Öztürk(2)
- Ucuncu, Osman(2)
- Şengül, Memnune(2)
- AKMEŞE, Osman(1)
Yayınlar
- MakaleYalnız künye2026Scopus SJRQ1Phytochemical profiling, biological activities, and molecular docking analysis of Dorycnium pentaphyllum subsp. haussknechtii
Ayna, Adnan; Hakan, Oguzhan; Yapıcı, İsmail; İzol, Ebubekir; Yılmaz, Mustafa Abdullah; Necip, Adem; Yapar, Yakup
BACKGROUND: In the current study, the biological activity fingerprint of Dorycnium pentaphyllum subsp. haussknechtii was investigated by an interdisciplinary approach through analysis of its phytochemical content, antioxidant capacity, enzyme inhibitory potential and molecular docking. RESULTS: The analysis of the phytochemical content by liquid chromatography–tandem mass spectrometry was characterized by the presence of flavan-3-ols, such as epicatechin, epigallocatechin, and catechin, as well as quercetin-derived flavonoids such as isoquercitrin. Analysis of the antioxidant capacity by 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ferric reducing antioxidant power, Fe3+–Fe2+ reducing and cupric reducing antioxidant capacity methods indicated the strong antioxidant capacity of the plant extract. In addition, the plant extract was able to modulate biological enzyme activity effectively, with IC50 values of 111.78 μg mL−1 for acetylcholinesterase, 22.07 μg mL−1 for butyrylcholinesterase, 1.39 μg mL−1 for α-glucosidase, 7.34 μg mL−1 for carbonic anhydrase I, and 17.41 μg mL−1 for carbonic anhydrase II. To have a better understanding of the molecular mechanism for the plant extract's properties, the major phenolic compounds present in the plant extract were investigated for molecular docking with their respective enzymes. As such, the results revealed a strong binding affinity and conformational stability, with strong hydrogen bonding and π–π interactions. CONCLUSION: Generally, the properties revealed by the plant extract indicate that the phenolic content, strong antioxidant properties, and enzyme inhibition activity of D. pentaphyllum subsp. haussknechtii have shown great potential for being a useful natural source for therapeutic lead compounds.
AtıfScopus1WoS1OpenAlex3İncele - MakaleYalnız künye2026Scopus SJRQ3Synthesis, antioxidant, and antiurease activities of novel benzimidazole-based 1,3,4-thiadiazole derivatives
Gümrükçüoğlu, Taner İlker; Akgün, Hakan; Bektaş, Hakan; Sökmen, Bahar Bilgin; Menteşe, Emre
In this study, we report the synthesis and characterisation of five new benzimidazole-based 1, 3, 4-thiadiazole derivatives (5a–e) and the evaluation of their antioxidant and antiurease activities. The structures were confirmed by NMR (1H, 13C) and LC/TOF–MS analyses (spectra are given in the supplementary information). Biological assays (DPPH, ABTS, iron-reducing power, urease inhibition) revealed that compound 5e (bearing a 4-nitrophenyl substituent) exhibited the strongest urease inhibition (EC50 = 0.1296 ± 0.0260 µM), surpassing thiourea. One-way ANOVA revealed significant differences in urease inhibition between the compounds (p<0.001). Post-hoc Tukey tests confirmed that compound 5e was more effective than several other compounds. The compounds exhibited moderate and comparable antioxidant activities. The first SAR study shows that electron-withdrawing substituents increase urease inhibition, whereas electron-donating groups increase the reducing power. Comparison with earlier reported triazole analogues shows scaffold-dependent changes in bioactivity. These findings support thiadiazoles as promising dual-function agents for oxidative stress and urease-associated pathologies.
- MakaleAçık Erişim2026Scopus SJRQ2Biochemical Profiles of Three Bryophytes: Sphagnum papillosum, Rhytidiadelphus triquetrus, and Ditrichum gracile
Okan, Onur Tolga; Baltaci, Cemalettin
This study compared Sphagnum papillosum, Rhytidiadelphus triquetrus, and Ditrichum gracile in terms of total phenolic content (TPC), total flavonoid content (TFC), antioxidant activities (DPPH, ABTS, ferric reducing antioxidant power [FRAP]), phenolic profiles, fatty acid compositions, and mineral contents. S. papillosum had the highest TPC (986.11 mg GAE/kg) and ABTS activity (9732.14 mg Trolox/kg). R. triquetrus showed the highest TFC (874.01 mg QE/kg) and was rich in protocatechuic (18 803 mg/g) and 4-hydroxybenzoic acids (23.219 mg/g). Although D. gracile had lower antioxidant activity, it contained high lipid (6.71%) and ash (27.66%) levels, along with substantial potassium (73 573.86 mg/kg) and calcium (26 135.94 mg/kg) accumulation. Palmitic acid (C16:0) was the dominant fatty acid in all species. These results demonstrate significant biochemical variation among the studied mosses, underlining their potential as sources of natural antioxidants, bioindicators of environmental stress, and candidates for bioremediation.
- MakaleAçık Erişim2026Scopus SJRQ1Enhancement of bioactive properties and antioxidant activity in wheat chips enriched with purple wheat flour
Yuksel, Ferhat; Kaya, Şemsettin
The objective of this study was to develop wheat chips fortified with purple wheat flour (PWF) and to evaluate the effects of PWF incorporation, frying temperature, and frying time on starch fractions, bioactive properties, antioxidant activity, and acrylamide formation. Response Surface Methodology was applied to assess the effects of PWF concentration (0%–100%), frying temperature (170–190 °C), and frying time (40–60 s) on the quality and functional properties of wheat chips. Resistant starch ranged from 0.51 to 0.80 g/100 g and increased slightly with higher PWF levels; this increase was found to be statistically significant. Acrylamide content was found to be a statistically significant parameter (p < .05), showing a decreasing trend from 1,362.68 to 1,082.76 μg/kg with increasing levels of PWF incorporation. Total phenolic, flavonoid, anthocyanin, and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH), free radical scavenging capacity (ABTS)) significantly improved with PWF enrichment, whereas ferric reducing antioxidant power showed no notable change. These findings indicate that PWF can be effectively incorporated into deep-fried wheat chips, enhancing their functional and bioactive properties while reducing acrylamide formation.
- MakaleAçık Erişim2026Scopus SJRQ1Effect of sloe berry (Prunus spinosa L.) powder addition on physicochemical, rheological, antioxidant, and sensory properties of stirred yoghurt: a multivariate approach
Yeniçeri, Esra; Ürkek, Bayram; Şengül, Mustafa
In this study, stirred yogurt was produced using sloe berry powder at different ratios (0, 2, 4, 6, and 8%) and stored for 21 days. The effect of sloe berry powder addition on physicochemical properties was limited, whereas the effect of storage period was more pronounced. Lactic cocci and lactic bacilli counts ranged between 7.78–8.93 log CFU/g and 6.19–9.13 log CFU/g, respectively. Yeast and mold were detected in all samples after 14 days of storage. All color parameters were significantly affected by sloe berry powder addition (p <.001), whereas storage period had no significant effect (p >.05). Total phenolic content and antioxidant capacity increased with increasing levels of sloe berry powder. Only the potassium (K) content was affected by the addition of sloe berry powder. Viscosity and consistency values increased in yogurts containing 6% and 8% sloe berry powder on days 14 and 21 of storage. Sensory scores decreased in all samples at the end of storage. Principal component and correlation analyses indicated that the addition of sloe berry powder and storage period exerted distinct effects on the evaluated parameters. Overall, sloe berry powder can be considered a promising ingredient for the development of nutritional and functional stirred yogurt.
- MakaleYalnız künye2026Scopus SJRQ2Phenolic Profiling of Laurus nobilis by LC–ESI–MS/MS: In Vitro Bioactivities and In Silico Enzyme Docking
Bak, Funda Erşen; Canbolat Gültekin, Deniz; Akmeşe, Osman; Atalay, Abdurrahman; Turan, İbrahim; Gültekin, Ergün; Demir, Selim; Aliyazicioglu, Yüksel
This study evaluated the branch, leaf, and fruit extracts of Laurus nobilis collected from Artvin in terms of phenolic composition, antioxidant, antimicrobial, and antidiabetic activities and additionally investigated the binding of selected phenolics to α-amylase/α-glucosidase targets through molecular docking. Using LC–ESI–MS/MS analysis, 31 phenolic compounds were quantitatively identified, with epicatechin, luteolin-7-O-glucoside, and rutin predominating particularly in the branches and leaves. Antioxidant capacity was higher in branches and leaves (total phenolic content [TPC]/FRAP/DPPH IC50: branches and leaves, respectively, 95.27–60.84 mg gallic acid equivalent [GAE]/g, 206.16–162.05 mg Trolox equivalent [TE]/g, and 46.95–113.49 µg/mL). Antimicrobial activity was observed within a minimum inhibitory concentration (MIC) range of 1.25–10 mg/mL; leaves exhibited the strongest effect against Bacillus cereus (1.25 mg/mL), whereas fruits were most effective against Candida albicans (1.25 mg/mL). In antidiabetic evaluation, fruits yielded the lowest IC50 values against α-amylase and α-glucosidase (4.31 and 8.97 mg/mL, respectively), suggesting a “composition-over-quantity” effect despite low total phenolic content. Docking results supported high binding affinities of rutin (α-glucosidase −9.3 kcal/mol; α-amylase −8.1 kcal/mol) and luteolin (−8.6 kcal/mol for both enzymes). Overall, although branches and leaves exhibited strong antioxidant profiles, fruits showed more pronounced inhibitory effects on digestive enzymes; therefore, fractionation studies and in vivo/kinetic validations are recommended.
AtıfScopus3WoS3OpenAlex6İncele - MakaleAçık Erişim2026Scopus SJRQ1Thermochemical Valorization of Silkworm Feeding Waste: Chemical Characterization and Biological Activities of the Pyrolysis-Derived Liquid Fraction
Karan, Halil; Yıldırım, Aybeniz; Yılmaz, Fırat; Özgen, İnanç; Aydoğmuş, Ercan; Gül, Abdulkadir
Silkworm-rearing residues are an underused biomass resource that can be converted into value-added products. In this study, a condensable liquid product (SWP-Liq) was produced from these residues by pyrolysis at 325 ± 5 °C and evaluated for its chemical composition and in vitro biological activities. SWP-Liq yielded phenolic-, flavonoid-, and triterpenoid-equivalent values of 218.47 ± 22.40 mg GAE/g, 79.13 ± 3.55 mg QE/g, and 55.54 ± 6.15 mg EE/g, respectively. The ABTS•+ assay showed a higher radical-scavenging response than DPPH, while Fe3+- and Cu2+-reducing activities were also observed. SWP-Liq inhibited 5-LOX with an IC50 of 33.05 ± 1.10 µg/mL, whereas α-glucosidase inhibition was weaker. Antibacterial activity against all four tested bacterial species was observed at 8 mg/mL but not at 4 mg/mL. Targeted LC–MS/MS quantified five phenolic constituents, with p-coumaric acid (1599.71 µg/g), chlorogenic acid (871.43 µg/g), and ferulic acid (285.50 µg/g) being the most abundant. Overall, these findings support the further investigation of silkworm feeding waste as a feedstock for thermochemical valorization into chemically and biologically evaluable bio-based fractions.
- MakaleAçık Erişim2026Scopus SJRQ4Assessment of the effect of conventional and contemporary drying methods on the phenolic profile modulation and antioxidant retention of Trabzon persimmon (Diospyros kaki L.)
Koçak, Tevfik; Demirel Ozbek, Yagmur; Saral, Ozlem; Kocyigit, Emine
This study investigated the effects of conventional and modern drying methods on the total phenolic content, antioxidant activity, and phenolic compound composition of Trabzon persimmons (Diospyros kaki L.) fruits harvested from the same tree and at comparable maturity stages were subjected to sun drying, shade drying, oven drying, air-fryer drying, and microwave drying techniques, with raw samples serving as the control group. The total phenolic content was assessed via the Folin–Ciocalteu method, and the antioxidant capacity was evaluated using the ferric reducing antioxidant power (FRAP) and DPPH radical scavenging activity assays. The identification and quantification of phenolic compounds were conducted using the HPLC technique. The results indicated that the drying process produced statistically significant variations in the phenolic content and antioxidant capacity of Trabzon persimmons. The microwave drying technique resulted in highest total phenolic content and antioxidant activity, whereas both sun and shade drying procedures showed higher values than raw samples. The total phenolic content and antioxidant capacity of samples dried in an air fryer were found to be comparable to those of raw samples. The profile of phenolic compounds varied according to the drying process employed; certain compounds exhibited an increase with specific methods, while others were identified solely under particular conditions. Thus, it has been shown that drying techniques can have a significant impact on preserving and improving the functional properties of persimmons. Furthermore, we believe that microwave drying could be an effective alternative, particularly in terms of preserving bioactive compounds.
AtıfScopus1OpenAlex1İncele - MakaleYalnız künye2026Scopus SJRQ2LC–MS/MS Phenolic Profiling and Antioxidant, Antimicrobial, and Antidiabetic Activities of Caucasian Propolis
Gültekin, Deniz Canbolat; Turan, İbrahim; Akmeşe, Osman; Gültekin, Ergün; Demir, Selim; Aliyazicioglu, Yüksel
Propolis is a natural bee product rich in phenolic and flavonoid compounds and is well known for its remarkable biological properties. In this study, the phenolic compositions of propolis samples collected from various localities in the Caucasus region of Türkiye were characterized using LC–MS/MS, and their antioxidant, antidiabetic, and antibacterial activities were also evaluated. LC–MS/MS analyses identified caffeic acid, ferulic acid, and p-coumaric acid as the major phenolic acid compounds, while pinocembrin, chrysin, and galangin were determined as the major flavonoid constituents. Overall, these findings indicate that the antioxidant capacity of propolis samples obtained from different regions may be particularly associated with these key phenolic and flavonoid compounds. Consistent with this composition, total phenolic and flavonoid contents ranged from 34.72 to 120.54 mg GAE/g and 6.88 to 47.19 mg QE/g, respectively. The extracts exhibited a relatively high antioxidant capacity, with FRAP values between 60.47 and 311.87 mg TE/g and DPPH IC50 values ranging from 215.80 to 37.96 µg/mL. Regarding antidiabetic activity, the IC50 values for α-amylase and α-glucosidase inhibition were determined as 5.86–3.47 and 12.22–5.02 mg/mL, respectively, indicating a pronounced antidiabetic potential. Antimicrobial analyses revealed activity against E. faecalis, B. cereus, E. coli, K. pneumoniae, and C. albicans. Overall, this study provides a comprehensive LC–MS/MS-based phenolic profile of Caucasian propolis and demonstrates its antioxidant properties together with its antidiabetic potential.
AtıfScopus2WoS2OpenAlex4İncele - MakaleAçık Erişim2026Scopus SJRQ3Green Synthesis and Bioactivities of Silver Nanoparticles Derived from Mercurialis annua L. Extract
Salad, Aisha Mohamud; Akar, Zeynep
Biogenic synthesis using plant materials provides a sustainable alternative to classical nanoparticle fabrication, minimizing chemical hazards and decreasing energy input. In this study, silver nanoparticles (AgNPs) were prepared using an aqueous extract of Mercurialis annua and characterized. Physicochemical properties of the AgNPs were confirmed using ultraviolet–visible spectroscopy (UV–Vis), Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), and transmission electron microscopy coupled with energy-dispersive X-ray (TEM–EDX), which collectively indicated crystalline, spherical silver nanoparticles stabilized by extract-derived functional groups. Subsequently, the biological activities of both the nanoparticles and the extract were comparatively evaluated using antioxidant tests, including cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic content (TPC), and total flavonoid content (TFC), as well as enzyme inhibition tests targeting α-glucosidase and carbonic anhydrase. Liquid chromatography-mass spectrometry profiling identified 31 phenolic constituents in the extract, with ferulic, caffeic, salicylic, and 4-hydroxybenzoic acids detected in high abundance. Overall, the biosynthesized AgNPs demonstrated higher antioxidant performance and stronger inhibitory effects on both enzymes compared with the crude extract. These results indicate that M. annua-based AgNPs hold considerable promise sustainable and effective bioactive agents for future biomedical and biotechnological applications.
- MakaleAçık Erişim2026Scopus SJRQ4Evaluation of Physicochemical Properties, Phytochemical Composition, Antioxidant Activity, Mineral Content, and Sensory Characteristics of Crackers Fortified with Olive Pomace
Altay, Kadriye; Akdoğan, Arda; Erdoğan, Selma Lubabe; Koç, Gülşah Çalışkan
Olive pomace (OP), a by-product of olive oil production, is a rich source of various bioactive compounds. OP can be used as a value-added product for the development of functional food. The research aimed to produce functional crackers incorporating different levels of OP powder (ranging from 0% to 20%). Subsequent analysis involved evaluating the physicochemical and phytochemical compositions, antioxidant activity, mineral content, and sensory characteristics of all samples. In light of the findings, the samples containing OP powder are rich in phytochemicals (total phenolic and flavonoid contents), with higher antioxidant activities (total antioxidant content, DPPH radical scavenging activity, ABTS radical scavenging activity, and ferric reducing power), as well as a high amount of minerals compared to the control sample (P<0.05). Moreover, as the concentration of OP increased, there was a decline in the moisture content, water activity, and color values of the crackers. Conversely, the ash, fat, total flavonoid, total phenolic, and mineral contents, as well as antioxidant activity and hardness values, exhibited an increase. Sensory analyses revealed that the addition of 10% OP powder produced highly acceptable crackers compared to the higher concentration of OP (15 and 20%). Overall results indicated that functional crackers can be produced by the addition of powder and a maximum of 10% OP powder fortification is recommended for consumer acceptability.
- MakaleAçık Erişim2026Scopus SJRQ2Evaluation of antioxidant and antiproliferative activities of shogaol and its modulatory effects on key metabolic enzymes
Yapıcı, İsmail; Gülçin, İlhami
Phenolic compounds are versatile bioactive molecules, particularly in food applications. In this study, the antioxidant capacity of Shogaol, a member of the phenolic class, was evaluated using several methods. Additionally, its inhibitory effects on human carbonic anhydrase I and II (hCA I and II), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glycosidase, and α-amylase enzymes, as well as its antiproliferative effects on A-549 lung cancer, HT-29 colon cancer, MCF-7 and MDA-MB-453 breast cancer cell lines, and the 3T3-L1 mouse fibroblast normal cell line, were investigated. To determine the antioxidant capacity of Shogaol, DPPH and ABTS radical scavenging assays, as well as Cu²⁺, Fe³⁺, and FRAP reduction methods, were employed. Its inhibitory effects on hCA I and II, AChE, BChE, α-glycosidase, and α-amylase enzymes were examined. Furthermore, cell counting was performed using the TBE method, and cell viability was measured with the XTT assay to assess the antiproliferative effects of Shogaol on A-549, HT-29, MCF-7, MDA-MB-453, and 3T3-L1 cell lines. Shogaol demonstrated higher antioxidant capacity than BHA, BHT, and Trolox in the FRAP assay (60 µg/mL), higher than BHT in the DPPH scavenging assay, and higher than Trolox in the ABTS, Cu²⁺, and Fe³⁺ reduction assays. Shogaol also exhibited significant inhibitory effects against hCA I, hCA II, AChE, BChE, and α-glycosidase, with IC₅₀ values of 75.91, 47.04, 37.44, 24.11, and 35.72 nM, respectively. Moreover, Shogaol showed a more potent antiproliferative effect than the standard chemotherapeutic drug Carboplatin in A-549 lung cancer and HT-29 colon cancer cell lines, and greater efficacy than Docetaxel in the MCF-7 breast cancer cell line.
AtıfOpenAlex1İncele - Yüksek Lisans TeziAçık Erişim2025Mercurialis annua L.'den gümüş nanopartiküllerin biyosentezi, karakterizasyonu ve biyolojik aktivitelerinin belirlenmesi
Aısha Mohamud Salad
Bu çalışma, Mercurialis annua L. bitki ekstraktı kullanılarak çevre dostu bir yöntemle gümüş nanopartiküllerin (AgNP'ler) sentezini incelemekte ve elde edilen nanopartiküllerin karakteristik özellikleri ile biyolojik aktivitelerini değerlendirildi. Geleneksel nanoparçacık üretim yöntemleri genellikle zararlı kimyasalların kullanımını ve yüksek enerji tüketimini gerektirir; bu durum hem çevresel hem de sağlık açısından riskler taşır. Buna karşılık, bitki bazlı biyosentez yöntemleri sürdürülebilir ve doğa dostu bir alternatif sunar. Bu kapsamda, fenolik bileşiklerce zengin olan tıbbi bitki Mercurialis annua L.'nin sulu ekstraktları, indirgeme ve dengeleme ajanı olarak kullanılmıştır. Elde edilen AgNP'ler, optik özelliklerini, kristal yapılarını, morfolojilerini ve elementel bileşimlerini belirlemek amacıyla UV-Vis spektroskopisi, FTIR, XRD ve TEM-EDX analiz yöntemleriyle karakterize edilmiştir. Nanopartiküllerin biyolojik potansiyeli, antioksidan etkinlik testleri DPPH, FRAP, CUPRAC, toplam fenolik (TPC) ve toplam flavonoid (TFC) madde içerik tayinleri ile birlikte α-glukozidaz ve karbonik anhidraz enzimlerini hedef alan inhibitör testleri aracılığıyla değerlendirilmiştir. Ayrıca, bitki özütü ve nanopartikül çözeltisinde yer alan temel fenolik bileşenlerin tanımlanması amacıyla LC-MS/MS analizi uygulanmıştır. Elde edilen bulgular, Mercurialis annua ekstraktının kararlı AgNP'lerin sentezinde etkili olduğunu ve bu nanopartiküllerin dikkate değer düzeyde antioksidan aktivite ve enzim inhibitör özelliği sergilediğini ortaya koymuştur. Bu çalışma, yeşil nanoteknoloji alanına katkı sağlamakta olup, bitki kaynaklı malzemelerin fonksiyonel nanomalzeme üretiminde taşıdığı önemi vurgulamaktadır.
- Yüksek Lisans TeziAçık Erişim2025Kuşburnu meyvelerinin farklı çözücüler içeren maserasyon ve ultrasonik destekli ekstraksiyon parametrelerinin antioksidan antimikrobiyal aktiviteleri ve enzim inhibisyonu etkilerinin karşılaştırılması
Mohammed Abdulsamad Hafedh
Tıbbi ve aromatik bitkiler uzun zamandır insanlığın yararına kullanılmaktadır. Bu bitkilerden en iyi şekilde yararlanmak için özütlerinin en doğru şekilde elde edilmeleri önem taşımaktadır. Kuşburnu (Rosa canina L.) yaygın biçimde tüketilen ve içerdiği fenolik bileşikler sayesinde önemli antioksidan, antimikrobiyal, antidiyabetik, anti-inflamatuar etkiye sahip yüksek derecede C vitamini içeren tıbbi bir bitkidir. Bu çalışmada ekstraksiyon için geleneksel maserasyon ve ultrasonikasyon destekli ekstraksiyon (maserasyon + ultrasonikasyon) yöntemleri uygulanmıştır. Bu amaçla dimetil sülfoksit (DMSO), Metanol-Su (20:80) ve etilasetat çözücülerinin kullanıldığı maserasyon ve maserasyon + ultrasonikasyon ekstraktları (sırası ile DMM, DMU, MSM. MSU, EAM ve EAU)oluşturuldu. Maserasyon + Ultrasonikasyon yönteminde maserasyon yöntemine ek olarak ultrasonikasyon gerçekleştirildi. Diğer tüm uygulamalar aynı şekilde yapıldı. Ekstraktların toplam fenolik madde (TPC), toplam flavonoid madde (TFC) içerikleri ile DPPH, ABTS ve FRAP olarak antioksidan aktivite tayinleri yapıldı. DMU ekstraktının diğer gruplara göre TPC ve TFC içeriği bakımından en yüksek değerlere sahip olduğu görüldü (p<0.05). Aynı şekilde DMU FRAP haricinde en yüksek antioksidan aktiviteye sahipti. Bu ekstraktlarda aynı zamanda Gram (-), Gram (+) bakteri suşlarını ve mayadan oluşan 8 farklı tür organizma ile antimikrobiyal aktivite ve minimal inhibitör konsantrasyon (MIC) tayini yapıldı. Genel olarak ekstraktların etki gösterdiği mikroorganizma sayısı değişmekle beraber Candia albicans ve Bacillus sereus'a karşı tüm ekstraktların antmikrobiyal aktiviteye sahip olduğu tespit edildi. Ekstraktların aynı zamanda ekstraktların astilkolinestreaz ve butirilkolinestreaz inhibitör aktiviteleri de araştırıldı. EAU'in en düşük AChE ve BChE inhibitör IC50 değerine sahip olduğu görüldü. Özellikle antioksidan içerik ve aktivite bakımından maserasyon ve ultrasonik destekli maserasyon yönteminin sadece maserasyon yöntemine göre polar çözücü kullanıldığında daha etkili olduğu tespit edildi.
- Yüksek Lisans TeziAçık Erişim2025Endemik erodium hendrikii alpınar'ın in vitro biyolojik aktivitelerinin araştırılması
İbrahim Koç
Erodium cinsi Geraniaceae familyasına aittir ve ülkemiz florasında 16'sı endemik olmak üzere 26 taksonla temsil edilmektedir. Erodium türleri, geleneksel olarak halk tıbbında çeşitli rahatsızlıkların hafifletilmesinde kullanılmaktadır. Ancak, standart olmayan bu gelişi güzel kullanımlar, bitkinin söz konusu etkilerinin bilimsel verilerle doğrulanması ve en etkili kullanım biçimlerinin ortaya konması ihtiyacını da beraberinde getirmiştir. Bu türlerden Gümüşhane'ye endemik olan Erodium hendrikii Alpınar (Güdük iğnelik)'ın biyolojik aktiviteleri hakkında sınırlı çalışmalar bulunmaktadır. Bu çalışma ile, E. hendrikii'nin farklı kısımlarından (çiçek, yaprak, kök ve kök kabuğu) farklı ekstraksiyon çözücüleri kullanılarak (%100 ve %70 etanol) elde edilen ekstraktlarının toplam fenolik, flavonoid içeriğinin, antioksidan, antimikrobiyal ve antikanserojen aktiviteleri araştırılmış ve ayrıca LC-MS/MS analizi ile toplam fenolik içeriği aydınlatılmıştır. Özellikle, bitki ekstraktlarının antimikrobiyal ve antikanser aktivitesi ilk defa araştırılmıştır. Elde edilen bulgulara göre, bitkinin toprak üstü kısmı olan Ç1 (Çiçek-%100 EtOH), Y1 (Yaprak-%100 EtOH) ve Ç2 (Çiçek-%70 EtOH), Y2 (Yaprak-%70 EtOH) ekstraktlarının biyolojik aktivitelerinin diğer ekstraktlardan (toprak altı) daha yüksek olduğu bulunmuştur. LC-MS/MS sonuçlarına göre majör fenolik bileşen ellagik asit (23142.738 µg/g ekstrakt), gallik asit (4230.406 µg/g ekstrakt) ve rutin (5639.335 µg/g ekstrakt) olarak tespit edilmiştir. Öte yandan, Ç1 ve KK2 (Kök Kabuğu-%70 EtOH) hariç, tüm ekstraktların 1 µg/mL MİK değeri ile Y. pseudotuberculosis'a karşı en yüksek antimikrobiyal etkinliğe sahip olduğu görülmüştür. Ayrıca Y1 Ekstraktının insan meme kanseri hücrelerine karşı konsantrasyona bağlı bir şekilde antiproliferatif özellikler gösterdiği ortaya konmuştur. Tüm sonuçlar birlikte değerlendirildiğinde, E. henrikii'nin sahip olduğu biyolojik aktiviteleri ile doğal, ucuz ve alternatif bir kaynak olarak farmasötik ve gıda endüstrilerinde kullanılma potansiyelinin oldukça yüksek olduğu düşünülmektedir.
- Yüksek Lisans TeziAçık Erişim2025Gelişmiş ve geleneksel yöntemlerle elde edilen sinirli ot (Plantago lanceolata) bitki ekstraksiyonlarının biyoaktif bileşenlerinin incelenmesi
Eren Çakar
Yapılan çalışmada sinirli ot (Plantago lanceolata) bitkisinin geleneksel ve gelişmiş (mikrodalga ve ultrases) ekstraksiyon yöntemleriyle ekstraktları hazırlanmış ve bu ekstraktların biyoaktif bileşenleri incelenmiştir. Ekstraktların antioksidan aktiviteleri Radikal süpürme kapasitesi (DPPH), Ferrik İyon İndirgeme (FRAP), Radikal nötralizasyonu (ABTS) şeklinde 3 farklı test yöntemiyle belirlenmiştir. Sinirli ot bitkisinin toplam fenolik ve flavonoid madde içeriği standart gallik asit ve kuarsetin kullanılarak analiz edilmiştir. Çalışılan antioksidan test yöntemleri arasından DPPH ve ABTS yöntemlerinde laboratuvar tipi mikrodalga ekstraktının diğer ekstraktlara göre daha yüksek sonuç verdiği belirlenmiştir. Başka bir antioksidan test yöntemi olan FRAP yönteminde ise su ekstraktının diğer ekstraktlara göre daha yüksek sonuç (2.02 ± 0.02A mg/g FeSO4) gösterdiği elde edilen sonuçlar arasındadır. Sinirli ot bitkisinin en yüksek toplam fenolik madde içeriği laboratuvar tipi mikrodalga ekstraktında (2.70 ± 0.13A mg/g GAE) olduğu belirlenirken toplam flavonoid içeriği en yüksek olan ekstraktın ise metanol ekstraktı (13.01 ± 0.27A mg/g QE) olduğu söylenebilir. Yapılan çalışma sonucunda sinirli ot bitkisinde yaş örneklerinde bulunan biyoaktif bileşiklerin, gelişmiş ekstraksiyon yöntemleriyle elde edilen ekstraktlarla yapılan analizlerinde daha etkili olduğu çalışmanın en belirgin sonuçlarındandır. Aynı zamanda sinirli ot bitkisinin antimikrobiyal aktivitesi B. cereus, E. coli, Y. enterocolitica, P. aeruginosa, S. typhimurium, C. albicans'a karşı disk difüzyon yöntemiyle belirlenmiştir. Elde edilen verilere göre bütün ekstraksiyonların C. albicans'a herhangi bir antimikrobiyal aktivitesinin olmadığı, en yüksek P.aeruginosa'ya karşı (17.6 0.01 mm), en düşük ise S. typhimurium'a karşı (6.6 0.01 mm) antimikrobiyal aktivite gösterdiği belirlenmiştir. Anahtar Kelimeler: Antimikrobiyal aktivite,Antioksidan aktivite, Biyoaktif bileşikler, Ekstraksiyon yöntemleri, Plantago lanceolata
- MakaleYalnız künye2025Scopus SJRQ2Phytochemical Profile, Antioxidant Capacity, Acetylcholinesterase, and Butyrylcholinesterase Enzyme Inhibition Properties of Myosotis amoena Boiss
Yapici, Ismail; Kayaoglu, Esma; Izol, Ebubekir; Yilmaz, Mustafa Abdullah; Behçet, Lutfi; Cakir, Oguz; Gülçin, Ilhami
Myosotis amoena Boiss. (Turkish name: Ho & scedil; boncukotu) is a perennial herbaceous plant that produces blue flowers during the spring. Phytochemicals, which are secondary metabolites distributed across plants, fungi, microorganisms, and even animal tissues, play crucial roles in health through their antioxidant and bioactive properties. Their antioxidant capacity largely arises from hydroxyl groups and aromatic ring structures that stabilize free radicals via electron delocalization. Enzyme inhibition is a therapeutic strategy in several diseases, including Alzheimer's disease (AD), where acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors are employed to manage symptoms. This study investigated the phytochemical composition (via LC-MS/MS), total phenolic content, antioxidant capacity, and AChE/BChE inhibitory effects of the ethanol extract of M. amoena using four in vitro methods. The CUPRAC assay demonstrated that M. amoena (1.070) exhibited higher antioxidant activity than Trolox (0.894), although its activity in FRAP, ABTS, and Fe3+ reduction assays was lower compared to standard antioxidants. The extract contained 53.03 mg GAE/g extract of total phenolics, with rosmarinic acid being the predominant compound (15.27 mg/g). Furthermore, the extract strongly inhibited AChE and BChE, with IC50 values of 3.9 and 19.8 mu g/mL, respectively. These findings highlight, for the first time, the significant bioactive potential of M. amoena as a natural source of antioxidants and cholinesterase inhibitors, supporting its prospective use in the prevention or management of neurodegenerative conditions.
AtıfWoS4OpenAlex8İncele - MakaleAçık Erişim2025Scopus SJRQ1Unveiling the bioactive potential of Scabiosa rotata M. Bieb fruits: a landmark study on cytotoxic, antioxidant, and antibacterial activities through liquid chromatography-mass spectrometry profiling and molecular docking
Yildirim, Aybeniz; Celep, Inci Kurt; Gül, Abdulkadir; Eter, Özge Soylu; Baǧcl, Eyüp
The aim of this study was to investigate the antioxidant, cytotoxic, and antibacterial activities of ethanol extract (SRE) and its hexane (SRH), chloroform (SRC), ethyl acetate (SREA), and aqueous ethanol (SRAE) sub-extracts obtained from the fruits of Scabiosa rotata M. Bieb. Molecular docking studies were also performed to evaluate the interactions of the most active sub-extract with the human mitochondrial ABC transporter (ABCB10) protein, plays an important role in cellular oxidative stress regulation and mitochondrial function. Total phenolic (TPC) and flavonoid (TFC) contents and antioxidant radical scavenging activities (1, 1-diphenyl-2-picryl hydrazyl [DPPH] and 3-ethylbenzothiazoline-6 sulfonic acid [ABTS]) of all extracts were determined. Cytotoxic activity was evaluated on different cell lines, such as HepG2 (liver cancer), MCF-7 (breast cancer), and HeLa (cervical cancer) cells. Antibacterial activity was tested against Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, and Staphylococcus aureus. SREA showed the best antioxidant activity with IC50 values of 54.20 μg/ml and 48.56 μg/ml for DPPH and ABTS, respectively, and also had high TPC (499.06 mg GA/g) and TFC (327.45 mg QE/g). Furthermore, SREA showed significant cytotoxicity comparable to cisplatin with IC50 values of 51.29, 51.41, and 52.89 μg/ml for HepG2, MCF-7, and HeLa cells, respectively. However, none of the extracts showed antibacterial activity. The analysis using liquid chromatography-mass spectrometry (LC/MS-MS) on SREA found that the main phenolic compounds are chlorogenic acid, hesperidin, quinic acid, and isoorientin. Molecular docking of the major compounds showed that chlorogenic acid (2.21 mM) exhibited the strongest binding affinity, followed by hesperidin (5.83 mM), quinic acid (8.86 mM), and isoorientin (13 mM). These findings indicate the potential of SREA to be used as a natural antioxidant and anticancer agent.
AtıfScopus7WoS7OpenAlex7İncele - MakaleAçık Erişim2025Scopus SJRQ2Enzyme inhibitory, antioxidant, antimicrobial activities, and phenolic profiles of the methanol extract of Gelasia sericea an endemic species
AYGÜL, İmdat; AKMEŞE, Osman; SEFALI, Abdurrahman; BALTACI, Cemalettin; ÖZ, Mehmet; FIDAN, Muhammed Said
This study aimed to investigate, for the first time, the phytochemical composition, antioxidant, antimicrobial, and enzyme inhibitory properties of the methanolic extract of Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N.Kilian. The extract demonstrated a moderate yield (6.55 g/100 g dry plant material) and was found to be rich in phenolic acids, particularly ferulic acid (17182.97 μg g-1), followed by caffeic acid, protocatechuic acid, and p-coumaric acid. Antioxidant analyses revealed high total phenolic (388.86 mg GA eq./100 g), total flavonoid (74.03 mg QE eq./100 g), and total antioxidant capacity (503.33 mg AA eq./100 g). The extract exhibited remarkable ABTS radical scavenging activity (95.99%, IC50 = 37.59 mg ml-1) and considerable ferric-ion reducing power (974.78 mg FeSO4 eq./100 g). Antimicrobial assays indicated moderate inhibitory effects, with stronger activity against Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis) and the yeast Candida albicans, whereas Gram-negative bacteria were less susceptible. The extract’s MIC values ranged between 0.20-3.25 mg ml-1, and MLC values between 0.41-6.50 mg ml-1, which were higher than those of standard antibiotics. Enzyme inhibition assays revealed moderate activity against carbonic anhydrase II (IC50 = 0.0136 µg ml-1) and weak inhibition of cholinesterases and α-glucosidase, while α-amylase inhibition was relatively more pronounced (IC50 = 13350.00 µg ml-1). Overall, these findings highlight G. sericea as a valuable natural source of phenolic acids and antioxidants, with promising potential for applications in functional foods, nutraceuticals, and phytopharmaceuticals. However, the relatively limited antimicrobial and enzyme inhibitory activities suggest the need for further studies on bioavailability, in vivo efficacy, and compound isolation to optimize its practical applications.
- MakaleYalnız künye2025Scopus SJRQ4Synthesis of New Triazole Compounds: Determination of Antiurease and Antioxidant Activities
Gümrükçüoğlu, T.İ.; Akgün, H.; Sökmen, B. Bilgin; Bektaş, H.
Abstract: Objective: In this study, we synthesized five novel triazole derivatives and biologically evaluated their antiurease and antioxidant properties. Methods: Five novel 1,2,4-triazole derivatives (5a–5e) were synthesized and tested for their urease inhibition and antioxidant activities using spectrophotometric methods. Results and Discussion: All compounds exhibited varying degrees of biological activity, with compound 5c demonstrating the most potent urease inhibition and radical scavenging capacity. Statistical analysis confirmed significant differences among the compounds, particularly highlighting the superior performance of 5c. Conclusions: These results suggest that halogen-substituted triazoles are promising candidates for further pharmacological development aimed at oxidative stress-related conditions and urease-associated pathologies.
AtıfScopus1WoS1OpenAlex1İncele
