Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Characterization and Bioactive Properties of Exopolysaccharides from Pediococcus ethanolidurans Isolated from Kırçan (Smilax excelsa L.) Pickles
Karatas, Seyda Merve; Genc, Berna; Tunc, Merve Tugce
In this study, the characterization of some bioactive content of exopolysaccharide (EPS) extracted from Pediococcus ethanolidurans isolated with Smilax excelsa L. from conventionally produced pickles were investigated. Background: Although this study is the main study involving the characterization of EPS obtained from Pediococcus ethanolidurans, it is important because it has determined a natural polysaccharide that can be used in different fields in the industry. According to the obtained results, sugar analysis by GC-MS revealed that EPS consisted of glucose (7.59%), mannose (41.96%), fructose (16.98%), arabinose (3.15%) and rhamnose (30.30%). From the thermal behavior determined by differential scanning calorimetry (DSC), it was concluded that it should not be heated close to 250 °C. At the same time, according to the thermogram obtained as a result of X-ray diffraction (XRD) analysis, it was found to have a crystalline structure. EPS, which reached 73.844% efficiency, showed antibacterial activity against Listeria monocytogenes, Staphylococcus aureus and Escherichia coli, while 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging activity was detected at 0.24 mM. It also has remarkable results, seen in cytotoxicity analysis against healthy HT-29 cells, demonstrating that it has proliferative activity as high as %125 In short, Pediococcus ethanolidurans was found to be a novel EPS producer with impressive properties.
- MakaleAçık Erişim2024Enterococcus durans SL70, a Novel Exopolysaccharide Producer from Traditional Sourdough Fermentation of Einkorn (Triticum monococcum L. ssp. monococcum)
Genc, Berna; Karatas, Seyda Merve; Tunç, Merve Tuğçe
Research background. Given the potential of microbial exopolysaccharides from lactic acid bacteria in various industrial processes, alternative sources for the isolation of lactic acid bacteria are highly topical. In this study, we used a traditional sourdough from einkorn (Triticum monococcum L. ssp. monococcum) as a source of lactic acid bacteria for the isolation, identification and determination of exopolysaccharide producers. Experimental approach. The sourdough was prepared from einkorn according to the traditional method. Lactic acid bacteria were isolated and purified using the single colony technique on MRS and M17 media. The isolates were characterised using matrix-assisted laser desorption ionization-time of flight mass (MALDI-TOF) spectrometry. All isolates were analysed for extracellular polysaccharide production and one isolate was selected for purification and characterisation of its polysaccharide. Results and conclusions. The isolates were identified as Lactobacillus plantarum, L. paraplantarum, L. brevis, Pediococcus pentosaceus, Enterococcus faecium and E. durans. The production of exopolysaccharides by all lactic acid bacteria was evaluated and it was found that all strains (except one) were capable of producing exopolysaccharides. One polysaccharide (EPS-SL70) was purified from the isolates of E. durans SL70. This anionic heteropolysaccharide had, in addition to the carbohydrate backbone, a protein structure that did not contain nucleic acid. The carbohydrate backbone consisted of mannose, glucose, rhamnose, arabinose, xylose and galactose. Novelty and scientific contribution. The microbial flora of traditional einkorn sourdough has been identified in this study and represents the first report on the exopolysaccharide production by lactic acid bacteria in traditional einkorn sourdough. Additionally, Enterococcus durans from einkorn sourdough was identified as a new exopolysaccharide producer.
- MakaleYayıncıda açık erişim2023Antimicrobial, Antioxidant, and Phytochemical Activities of Rhus coriaria L. and its Phenolic Compounds and Volatile Component Analyses
Öz, Mehmet; Baltaci, Cemalettin; Fidan, Muhammed Said; Karatas, Seyda Merve
Volatile oil analysis, phenolic constituents, antioxidant capacity, antimicrobial activity, vitamin C, and enzyme activities of the fruits of Rhus coriaria L. were studied. The chemical with the highest percentage was sesquiterpene hydrocarbons with 40.4%. The major compound was detected as caryophyllene (36.9%). The main phenolic constituents of fruit samples were gallic acid, syringic acid, protocatechuic acid, and 4hydroxybenzoic acid. The highest phenolic constituent of fruits was gallic acid. Ferric (Ill) ion reducing antioxidant power (FRAP) capacity (14.9 mg FeSO4 eq./g), free radical scavenging (ABTS) capacity (68.8 mg AA eq./g), ABTS % inhibition rate (98.0%), free radical scavenging (DPPH) (53.1 mg AA eq./g), and DPPH % inhibition (79.6%) amounts were determined in antioxidant capacities of the samples. The bioactive component contents of the samples were total antioxidant amounts (TAC) (32.8 mg GA/g), total flavonoid substance amounts (TFC) (73.8 mg QE eq./g), and total phenolic substance amounts (TPC) (41.4 mg GA eq./g). The results of the antimicrobial activity analysis of R. coriaria fruit samples showed antimicrobial activity against Staphylococcus aureus and Listeria monocytogenes microorganisms. The amount of vitamin C and enzyme inhibitor activity in the fruits of R. coriaria were determined as 35.5 mg/100 g and 0.07 mg/mL, respectively.
- MakaleYalnız künye2023Phenolic Components, Mineral Composition, Physicochemical, and Bioactive Properties of Opuntia ficus-indica with Different Drying Methods
Capar, Tugba Dursun; Dedebas, Tugba; Kavuncuoglu, Hatice; Karatas, Seyda Merve; Ekici, Lutfiye; Yalcin, Hasan
Cactus pear fruit (Opuntia ficus-indica) have nutritional and antioxidant properties due to the presence of vitamins, fibers, minerals, and polyphenols. The present paper aims to examine the effects of drying methods (sun, oven, vacuum, and freeze) on the bioactivity and antimicrobial quality of prickly pear fruit. Total phenolic content (TPC), antioxidant (DPPH) and antimicrobial activity, mineral content, phenolic compounds, and color values were investigated. The highest antioxidant activity (DPPH) and color values were observed in the freeze-dried samples, which were very close to the values for fresh samples. The total phenolic content (TPC) of the freeze-dried sample was 503.53 mg GAE/100 g. The most abundant phenolic compound was p-hydroxybenzoic acid (210 ppm) identified in fresh and freeze-dried fruit extract, which may be more effective antimicrobials than the rest. The mineral content of freeze- and vacuum-dried samples was very close to that of fresh fruit. This study indicates that freeze-drying of prickly pear fruit improved the bioactive and antimicrobial properties without leading to nutritional loss.
- MakaleAçık Erişim2023Chemical composition and biological activities of essential oils from the flowers and leaves of Celtis planchoniana K.I.Chr.
Öz, Mehmet; Ucuncu, Osman; Baltacı, Cemalettin; Said Fidan, M.; Karatas, Seyda Merve
Most plant parts are used in traditional food and medicine. Plants are significant resources of potential phytochemicals. This study investigated the properties of essential oil, antioxidant, and antimicrobial activities of the flowers and leaves of the Celtis planchoniana plant. Based on the GC/MS-FID analysis of essential oils extracted from the flowers and leaves of C. planchoniana, 101 and 91 compounds were identified from their structures, respectively. Chemical classes with the highest percentage of compounds found in essential oils of plants were oil acids, with 31.51% in flowers, and aldehydes, with 32.89% in leaves. Hexadecanoic acid (29.94%) and (E)-2-hexenal (16.11%) compounds were determined as the main components in the plant’s flowers and leaves. Among the flower and leaf samples, the highest antioxidant levels were 132.77-80.10 mg AA eq./100 mL in TAC, 17.63-118.23 mg AA eq./100 mL in ABTS, 71.41-85.15 mg GA eq./100 mL in TPC, and 15.08-15.71 mg AA eq./100 mL in DPPH capacity. According to the TFC, TPC, ABTS, DPPH, and FRAP tests conducted to determine the antioxidant properties of C. planchoniana, the essential oil obtained from its leaves demonstrated more significant antioxidant properties than the essential oil obtained from its flowers. According to the antimicrobial activity analysis, the leaf and flower essential oil samples exhibited no antimicrobial activity against the microorganisms tested.
- MakaleYalnız künye2022COMPOSITION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES AND CHEMICAL COMPONENTS OF ESSENTIAL OIL FROM FLOWERS AND LEAVES OF Pyrus elaeagnifolia Pallas IN TURKEY
Fidan, Muhammed Said; Oz, Mehmet; Ucuncu, Osman; Baltaci, Cemalettin; Karatas, Seyda Merve
Gumushane region in Turkey is rich in plant diversity. The flowers and leaves of Pyrus elaeagnifolia Pall. plants that will form the basis of this research were collected from the places determined. In this study; the essential oils of flowers and leaves of P. elaeagnifolia plants were obtained by hydrodistillation method in a Clevenger type device. The chemical composition of volatile oils diluted with hexane were determined by analyzing with a GC-MS/FID instrument. The quantity of antioxidant activity of the obtained essential oils was determined by total phenolic content, free radical scavenging amounts, total flavonoid content, total antioxidant content, free radical scavenging capacity and ferric reducing antioxidant power capacity according to methods. Antimicrobial activity of essential oils was determined against 23 different microorganisms by disc diffusion method. As a result; essential oil yields in percentage are 0.44% and 0.72% in the flowers and leaves of the P. elaeagnifolia. Chemical classes with the highest percentage of compounds in essential oils of plant parts: Hydrocarbons (79.77%) and aldehydes (33.31%) were detected in the flowers and leaves of the P. elaeagnifolia. In addition, the main components found in essential oils are tricosane (36.98%-15.89%) in the flowers and leaves of the P. elaeagnifolia. The determination of antioxidant activity obtained from samples collected from plant parts was determined total phenolic substance amounts in the range of 48.57-68.32 mg GAE/100 mL, free radical scavenging amounts between 9.52-40.95 mg AA/100 mL, flavonoid substance amounts in the range of 1.34-3.95 mg QE/100 mL, total antioxidant amounts in the range of 66.93-185.10 mg AA/100 mL, free radical scavenging capacity between 23.40-81.02 mg AA/100 mL and FRAP capacity in the range of 4.20-10.50 mg FeSO4/100 mL. According to the results obtained with total phenolic content, free radical scavenging amounts, total flavonoid content, total antioxidant content, free radical scavenging capacity and ferric reducing antioxidant power capacity was determined the highest antioxidant properties in leaves of P. elaeagnifolia. According to the results of the antimicrobial activities analysis obtained from the collected samples of plant parts, it was determined that only the flowers of P. elaeagnifolia show antimicrobial activity against the microorganisms used.
- MakaleYalnız künye2021Determination of the Chemical Composition, Antioxidant and Antimicrobial Activities of Different Parts of Rosa canina L. and Rosa pimpinellifolia L. Essential Oils
Öz, Mehmet; Deniz, Ilhan; Okan, Onur Tolga; Baltaci, Cemalettin; Karatas, Seyda Merve
Today, the use of essential oils is increasing day by day, and it is considered as raw materials in many industries. Therefore, this study aims to identify the essential oil profile, antioxidant and antimicrobial activity of Rosa canina L. and Rosa pimpinellifolia L. reveal their potential use in the industry. Essential oils that were obtained in 2013-2014 from different parts (flower, leaf, stem, and fruit) of Rosa canina L. and Rosa pimpinellifolia L. by the hydro-distillation method were analyzed by GC/MS. The dominant components in Rosa canina L. were found as trans-geraniol in the flowers (42.08 % for 2013), linalool in the leaves (11.55 % for 2014), and stem (14.91 % for 2013) and vitispirane in the fruits (37.19 % for 2014). The dominant components in R. pimpinellifolia L. were found as heneicosane in the flowers (22.07 % for 2014), γ-elemene in the leaves (30.21 % for 2013), linalool in the stem (14.76 % for 2013) and the fruits, (E)-2-hexenal for those collected in 2013 (14.09 %). The IC50 and FRAP values of the samples ranged from 127.54 to 142.22 μg/g and 121.43 to 296.78 μmol TE/g, respectively. According to the results obtained, both R. canina L. and R. pimpinellifolia L. were found to show poor antioxidant values of DPPH and FRAP. In general, it was determined that the essential oils obtained from different plant parts of both species had a moderate antimicrobial effect against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, and Pseudomonas aeruginosa.
- MakaleAçık Erişim2021Determination of Antimicrobial and Antioxidant Activities and Chemical Components of Volatile Oils of Atropa belladonna L. Growing in Turkey
Öz, Mehmet; Fidan, Muhammed Said; Baltaci, Cemalettin; Ücüncü, Osman; Karatas, Seyda Merve
In this study; the essential oils were obtained from the flowers and leaves of Atropa belladonna L. plants through the hydrodistillation method in a Clevenger type device. The chemical composition of volatile oils was determined by analyzing with the GC-MS/MS instrument. The antioxidant activity of the obtained volatile oils was determined according to total antioxidant content (TAC), free radical scavenging (ABTS) capacity, free radical scavenging (DPPH) amounts, total flavonoid content (TFC), total phenolic content (TPC), and ferric reducing antioxidant power (FRAP) capacity methods. Antimicrobial activities of essential oils were determined through the agar-disc diffusion method. As for the results; volatile oil yields of the A. belladonna were determined as 0.37 % in leaves and 1.57 % in flowers. The highest percentage of chemical compounds in essential oils of the A. belladonna plant were determined as (43.75 %) aldehydes in leaves and as (39.96 %) fatty acids in flowers. In addition, the main constituents found in volatile oils were eicosane (35.92 %) in the leaves and hexadecenoic acid (18.84 %) in the flowers of the A. belladonna. The highest amounts of antioxidant for the test specimens were found as 1.37-1.48 mg QE/100 mL in TFC, as 13.25-21.91 mg AA/100 mL in ABTS, as 51.49-62.87 mg GA/100 mL in TPC, and as 4.71-7.97 mg FeSO4/100 mL in FRAP capacity. As the results of the antimicrobial activities, it was determined that only the leaves of A. belladonna show antimicrobial activity against the mold-yeast and gram-positive and gram-negative bacteria microorganisms among the 17 bacteria and 6 yeast-mold types used in the study.
- MakaleYalnız künye2021Effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides produced by yoghurt starter cultures
Karatas, Seyda Merve; Ekici, Lutfiye; Develi, Ibrahim; Dertli, Enes; Sagdic, Osman
In this study, the effects of GSM 1800 band radiation on composition, structure and bioactivity of exopolysaccharides (EPSs) produced by Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus were determined. For this, GSM 1800 band radiation was applied to both cultures and characteristics of EPSs extracted from the control groups (K) and the radiation stressed groups (R) were determined. An alteration in the chemical composition of the EPSs was observed and EPS production levels and molecular weights of the EPSs increased following the GSM 1800 band radiation application. Alterations in the functional groups, thermal and morphological characteristics of EPSs following the GSM 1800 band radiation application were confirmed by FTIR, TGA and SEM analysis, respectively. Importantly no alterations in the antioxidant and antibacterial activity of the EPSs were observed following the radiation application. These results suggested the effects of the GSM radiation on final characteristics of EPSs from yogurt starter cultures.
- MakaleYalnız künye2018Prediction of the antimicrobial activity of walnut (Juglans regia L.) kernel aqueous extracts using artificial neural network and multiple linear regression
Kavuncuoglu, Hatice; Kavuncuoglu, Erhan; Karatas, Seyda Merve; Benli, Büsra; Sagdic, Osman; Yalcin, Hasan
The mathematical model was established to determine the diameter of inhibition zone of the walnut extract on the twelve bacterial species. Type of extraction, concentration, and pathogens were taken as input variables. Two models were used with the aim of designing this system. One of them was developed with artificial neural networks (ANN), and the other was formed with multiple linear regression (MLR). Four common training algorithms were used. Levenberg–Marquardt (LM), Bayesian regulation (BR), scaled conjugate gradient (SCG) and resilient back propagation (RP) were investigated, and the algorithms were compared. Root mean squared error and correlation coefficient were evaluated as performance criteria. When these criteria were analyzed, ANN showed high prediction performance, while MLR showed low prediction performance. As a result, it is seen that when the different input values are provided to the system developed with ANN, the most accurate inhibition zone (IZ) estimates were obtained. The results of this study could offer new perspectives, particularly in the field of microbiology, because these could be applied to other type of extraction, concentrations, and pathogens, without resorting to experiments.