Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Demir, Selim(12)
- Turan, Ibrahim(11)
- Aliyazicioglu, Yuksel(8)
- Topal, Meryem(7)
- Şengül, Mustafa(6)
- Baltaci, Cemalettin(5)
- Topal, Fevzi(5)
- Öz, Mehmet(5)
- Ürkek, Bayram(5)
- Akar, Zeynep(4)
- Alwasel, Saleh H.(4)
- Durmaz, Lokman(4)
- Gulcin, Ilhami(4)
- Gülçin, Ilhami(4)
- Akmeşe, Osman(3)
- Bingol, Zeynebe(3)
- Gören, Ahmet C.(3)
- Karatas, Seyda Merve(3)
- Misir, Sema(3)
- Turan, İbrahim(3)
- Yapıcı, İsmail(3)
- Yildiz, Hilal(3)
- Aliyazicioglu, Yüksel(2)
- Altay, Kadriye(2)
- Baltacı, Cemalettin(2)
- Deger, Orhan(2)
- Fidan, Muhammed Said(2)
- Gocer, Hulya(2)
- Gül, Abdulkadir(2)
- Gültekin, Ergün(2)
- Gülçin, İlhami(2)
- Kalin, Pinar(2)
- Kiziltas, Hatice(2)
- Kose, Leyla Polat(2)
- Küçük, Murat(2)
- Okan, Onur Tolga(2)
- Sarikaya, S. Beyza Öztürk(2)
- Ucuncu, Osman(2)
- Şengül, Memnune(2)
- AKMEŞE, Osman(1)
Yayınlar
- MakaleAçık Erişim2025Scopus SJRQ1Evaluation of Antioxidant and Enzyme Inhibition Properties of Siran Propolis: Correlations With Phenolic Content Determined by LC–MS/MS
Balci, Neslihan; Aslan, Kubra; Erturk, Adem; Gulcin, Ilhami
This study evaluates the antioxidant activity, enzyme inhibition potential, and phenolic composition of ethanolic Siran propolis extract. Antioxidant capacity was measured using reducing power (CUPRAC, FRAP, and Fe3+-reducing) and radical scavenging (DPPH·, ABTS·+, and DMPD·+) assays. The extract showed strong reducing ability, surpassing standard antioxidants in CUPRAC and FRAP assays, correlating with high ferulic acid (40.40 mg/g) and p-coumaric acid (88.60 mg/g) levels identified by LC–MS/MS. While it demonstrated notable DPPH· scavenging (IC50: 30.14 μg/mL), ABTS·+ activity was weaker (IC50: 272.44 μg/mL). Enzyme inhibition assays indicated effective inhibition of α-glycosidase, AChE (IC50: 2.15 μg/mL), hCA I, and hCA II. Notably, AChE inhibition was stronger than the standard tacrine inhibitor (IC50: 8.82 μg/mL), suggesting neuroprotective potential. The observed bioactivities are attributed to synergistic effects of phenolic acids and flavonoids, including quercetin, luteolin, taxifolin, and resveratrol. These findings highlight Siran propolis as a promising natural antioxidant and enzyme inhibitor source with potential therapeutic applications and food formulations.
AtıfScopus12WoS12OpenAlex11İncele - MakaleAçık Erişim2025Scopus SJRQ3Evaluation of antibacterial, antioxidant activities and secondary metabolites profile of orchids with different dried methods
Ozkan, Ahmet Ismail; Canbolat-Gültekin, Deniz; Soruklu, Sudenaz; Işik, Uğur; Akmeşe, Osman
The Central Black Sea region is a very dense region in terms of orchid diversity. As a consequence of the floral studies, 32 species of salep orchids were identified in the Central Black Sea Region. The aim of this current study was to identify the secondary metabolite profiles of leaves and tubers of two orchid species (Anacamptis palustris and Ophrys apifera) with different drying methods by HPLC analysis and to determine their antibacterial and antioxidant activities. For this purpose, different drying methods were applied to the roots and leaves of these two species. The species were dried by 3 different techniques: shade, lyophilizer and oven. After the aqueous extracts were obtained, their antibacterial and antioxidant activities were tested and HPLC analysis was completed. The data obtained from aqueous extracts as a result of drying techniques were compared with the data obtained from fresh plant aqueous extracts. Antibacterial properties were tested against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 bacteria. No antibacterial activity was found in the study. Antioxidant properties were tested via DPPH and FRAP assays. As a result of DPPH free radical scavenging assay, the highest antioxidant efficiency in A. palustris was observed in leaves dried in lyophilizer (76.53%), in the shade (62.13%) and in the oven (54.12%), respectively. Nine different phenolic compounds were found in aqueous extracts of these species. Epicatechin, Gallic Acid, Rutin, Syringic Acid phenolic compounds were observed the most in HPLC analysis. The results of current study suggest that these extracts have antioxidant and phenolic contents and should be investigated in more comprehensive studies.
AtıfScopus2WoS1OpenAlex1İncele - MakaleAçık Erişim2025Scopus SJRQ2Production and quality assessment of functional crackers based on fruit seed oils
Barut, Yeliz Tekgul; Koç, Gulsah Caliskan; Akdogan, Arda; Erdogan, Selma Lubabe; Ergün, Ahsen Rayman; Altay, Kadriye; Rustagi, Sarvesh; Ramniwas, Seema; Pandiselvam, Ravi
Crackers are popular bakery products known for their crunchy texture and long shelf life. Typically made from leavened or unleavened dough with wheat flour, oil, water, and additives, crackers have undergone recipe enhancements to improve sensory and nutritional profiles. While many studies explore cracker and biscuit formulations, fruit seed oils remain underexplored. Fruit seeds, often discarded as waste, are rich in essential fatty acids, amino acids, and micronutrients. Oils from seeds such as grape, pomegranate, rosehip, and apricot have gained attention for their health benefits, which presents an opportunity for innovation in bakery products. This study evaluated the potential of incorporating various fruit seed oils (pomegranate, apricot kernel, grape, fig, and rosehip) into cracker formulations. Crackers were assessed for physicochemical properties, antioxidant activity, mineral content, texture, and sensory attributes, with sunflower oil as the control. Results showed that the addition of fruit seed oils significantly influenced cracker properties. Crackers with apricot kernel oil had the highest ash content (2.29 +/- 0.13%) and exhibited a more acidic character, as did those with grape seed oil. Moisture content decreased significantly when rosehip seed oil was used (P < 0.05). Grape seed oil yielded the highest water-holding capacity, while sunflower oil provided the highest oil-holding capacity. Crackers made with rosehip and apricot kernel oils demonstrated improved antioxidant properties. Using fruit seed oils not only enhances nutritional value (e.g. vitamins, minerals, antioxidants, and dietary fiber) but also reduces material waste, contributing to sustainability and better resource utilization.
AtıfScopus5WoS6OpenAlex6İncele - Yüksek Lisans TeziAçık Erişim2024Giresun yöresinde geleneksel yöntemlerle üretilen pekmez çeşitlerinin bazı fizikokimyasal, antioksidan ve spektroskopik özelliklerinin (FTIR) belirlenmesi
Ferdi Kerem Konal
Pekmez, ülkemizde çok eski zamanlardan beri tüketilen, çabuk bozulabilen meyvelerin işlenerek daha uzun raf ömrüne sahip olmasını amaçlayan geleneksel bir üründür. Üzüm sıklıkla pekmez üretiminde tercih edilen meyvelerin başında gelse de armut, taflan, dut gibi meyveler de pekmez üretiminde kullanılabilmektedir. Pekmez ülkemizin hemen hemen her bölgesinde yöredeki meyve çeşitliliğine göre hammaddesi farklılaşarak üretilebilen bir gıdadır. Geçmişten günümüze geleneksel üretiminin çok sık tercih edildiği bilinen pekmezin endüstriyel üretimi de ülkemizde son derece yaygındır. Literatürde pekmezdeki HMF miktarı, pekmezin asitliği, rengi, kıvamı, organik asit ve mineral içeriğinin önemli kalite kriterleri olduğu değerlendirilmektedir. Bunun yanı sıra şeker ilavesi ve farklı meyvelere ait pekmezlerin karıştırılması gibi uygulamalar da tağşiş riskini oluşturmaktadır. Bu tez çalışmasında, Giresun ilinde geleneksel yöntemlerle üretilen üzüm, taflan, dut ve armut pekmezlerinin fizikokimyasal özelliklerini belirlemek amaçlanmıştır. 4 farklı çeşit pekmezden numune toplanarak pekmez örneklerinde suda çözünür kuru madde, toplam kuru madde, renk, pH, titrasyon asitliği, kül, elektriksel iletkenlik, antioksidan kapasite, toplam fenolik kapasite, toplam flavanoid madde içeriği, HMF, FTIR spektoskopisi, şeker, esmerleşme düzeyi analizleri gerçekleştirilmiştir. Yapılan çalışmada örneklerin toplam antioksidan kapasitesi 297.58±2.00 mg/100 g ile 609.12±0.00 arasında, toplam fenolik kapasitesi 40.33±4.55 ile 138.88±2.55, toplam flavanoid kapasitesi 67.50±1.00 ile 2632.50±15.00 mg/100 g arasında değişmektedir. Pekmez örneklerinin HMF miktarı 5.97±0.04 ile 1827.76±17.51 mg/kg arasında değişmektedir. Esmerleşme düzeyi sonuçları 17.00±0.05 ile 110.00±0.04 arasında tespit edilmiştir. Pekmez örneklerinin FTIR spektrumları benzerdir.
- MakaleAçık Erişim2024Determination of physicochemical and antioxidant properties of icecream produced with the addition of Jerusalem artichoke (Helianthus tuberosus L.); [Yer elması (Helianthus tuberosus L.) ilavesiyle üretilen dondurmanın fizikokimyasal ve antioksidan özelliklerinin belirlenmesi]
Gündoğdu, Engin
The study investigates the effects of incorporating Jerusalem artichoke (Helianthus tuberosus L.) into ice cream formulations on their physicochemical and antioxidant properties of icecream. Jerusalem artichoke (JA), known for its high inulin content and beneficial carbohydrates, was added to ice cream in varying concentrations (5%, 10%, and 15%) and evaluated for its impact on dry matter, protein, ash, pH, acidity, sugar content, viscosity, volume increase, initial dripping time, total melting time, color values, total phenolic content, DPPH free radical scavenging activity, and total antioxidant capacity.The results revealed that the addition of JA significantly improved the dry matter and antioxidant properties of the ice cream, with the highest values observed in samples containing 10% and 15% JA. Total phenolic content increased from 363.51 mg GAE/kg in the control to 587.47 mg GAE/kg with 15% JA, while DPPH scavenging activity rose from 12.49% to 18.04%. On the other hand, the control sample has the highest total antioxidant capacity (4517.29 mg AAE/kg), while the JA samples generally exhibit lower values. The protein content decreased slightly with the addition of JA, while ash content increased. The pH and titratable acidity values also showed significant changes, with higher concentrations of JA resulting in higher pH and acidity. Overall, the incorporation of JA into ice cream formulations enhances the nutritional and functional properties. These findings suggest that JA is a valuable ingredient for developing functional ice cream with potential health benefits.
AtıfScopus2OpenAlex2TR Dizin1İncele - MakaleAçık Erişim2024Scopus SJRQ1Assessment of the antioxidant and ACE-inhibitory activities and some quality characteristics of Kaşar cheese produced by probiotic Lactobacillus acidophilus
Gürmeriç, Hüseyin Ender; Şengül, Mustafa; Erkaya-Kotan, Tuba
In this study, four types of Kaşar cheese were produced using raw milk (C), starter culture (Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus) (S), starter culture + probiotic Lactobacillus acidophilus ATCC 4356 (SLA), and probiotic L. acidophilus ATCC 4356 (LA) were produced and ripened at 6 ± 2 °C for 150 days. The physicochemical, microbiological, proteolysis, electrophoretic (Urea-PAGE), ACE-inhibitory, and antioxidant activity analyses of vacuum-packed Kaşar cheeses were performed on 5, 30, 60, 90, 120, and 150 days of ripening. During ripening, no significant differences (p > 0.05) were detected in total solids, fat, and protein values of Kaşar cheeses. Titratable acidity, water-soluble nitrogen (WSN), ripening index (RI), and electrophoresis results of cheese samples showed an increase while pH values decreased. Also, the degradation products of αs1-casein were higher than those of β-casein. The number of probiotic L. acidophilus ATCC 4356 after scalding decreased by an average of 1.57 log cfu/g. However, probiotic bacteria count increased during ripening and maintained their viability. The addition of L. acidophilus ATCC 4356 improved the bioactivity, electrophoresis, titratable acidity, and ripening index results of Kaşar cheese. ACE-inhibitory activity of Kaşar cheeses increased during ripening and had the highest value on the 150th day of ripening. Furthermore, antioxidant activity of Kaşar cheeses increased on the 60th day of ripening and then decreased. Consequently, the combination of probiotic bacteria and starter culture had a positive effect on the physicochemical, microbiological, and proteolytic properties of Kaşar cheese.
AtıfScopus10WoS9OpenAlex11İncele - MakaleAçık Erişim2024Scopus SJRQ1Evaluation of microbiological, antioxidant, thermal, rheological and sensory properties of ice cream fermented with kefir culture and flavored with mint (Menthaspicata L.)
Öztürk-Yalçın, Feyza; Ürkek, Bayram; Şengül, Mustafa
Kefir is a healthy fermented dairy product, while ice cream is one of the most consumed dairy products. In this research, ice cream was fermented using a commercial kefir culture and flavored with a mint aroma in different proportions: 0% (KI), 0.2% (KIM2), 0.4% (KIM4), and 0.6% (KIM6). The study investigated the microbiological, thermal, rheological, textural, compositional, and sensory properties as well as antioxidant activity of kefir ice cream samples during 45-day storage. The lactic bacilli, lactic cocci, and Leuconostoc counts of samples were >7 log CFU/g, while the yeast counts were <4 log CFU/g. The microbiological properties (except for yeast counts) of the samples were coherent with legal limits. Antioxidant values (except for samples KIM2 and KIM6) values did not indicate significant differences (p <.05). The pH and melting rate values of the samples decreased with the addition of mint flavor, while acidity values increased. The addition of mint aroma caused significant changes in the thermal properties of ice cream. The overrun, a*, WI, and hardness values of the samples decreased based on the mint flavor concentration, whereas the viscosity and rheological increased. Samples KI and KIM2 were scored higher than other samples for all sensory properties. As a result, fermented ice cream flavored (0.2% mint) with mint could be produced as a desirable dairy product with potential functional properties.
AtıfScopus8WoS6OpenAlex6İncele - MakaleAçık Erişim2023Scopus SJRQ2Chemical composition and biological activities of essential oils from the flowers and leaves of Celtis planchoniana K.I.Chr.
Öz, Mehmet; Ucuncu, Osman; Baltacı, Cemalettin; Said Fidan, M.; Karatas, Seyda Merve
Most plant parts are used in traditional food and medicine. Plants are significant resources of potential phytochemicals. This study investigated the properties of essential oil, antioxidant, and antimicrobial activities of the flowers and leaves of the Celtis planchoniana plant. Based on the GC/MS-FID analysis of essential oils extracted from the flowers and leaves of C. planchoniana, 101 and 91 compounds were identified from their structures, respectively. Chemical classes with the highest percentage of compounds found in essential oils of plants were oil acids, with 31.51% in flowers, and aldehydes, with 32.89% in leaves. Hexadecanoic acid (29.94%) and (E)-2-hexenal (16.11%) compounds were determined as the main components in the plant’s flowers and leaves. Among the flower and leaf samples, the highest antioxidant levels were 132.77-80.10 mg AA eq./100 mL in TAC, 17.63-118.23 mg AA eq./100 mL in ABTS, 71.41-85.15 mg GA eq./100 mL in TPC, and 15.08-15.71 mg AA eq./100 mL in DPPH capacity. According to the TFC, TPC, ABTS, DPPH, and FRAP tests conducted to determine the antioxidant properties of C. planchoniana, the essential oil obtained from its leaves demonstrated more significant antioxidant properties than the essential oil obtained from its flowers. According to the antimicrobial activity analysis, the leaf and flower essential oil samples exhibited no antimicrobial activity against the microorganisms tested.
AtıfScopus7WoS6OpenAlex5İncele - MakaleYalnız künye2023Scopus SJRQ3Determination of the Antioxidant Capacity of Puerarin and Its Effect on Cholinesterases by in vitro and in silico Methods
İnan Ergün, Aslı; Topal, Meryem
Puerarin, which was used as a natural isoflavone in this study, was obtained from the Kudzu plant. The inhibitory effect of puerarin on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes was investigated. The IC50 values of AChE (177.73 nM) and BChE (315.07 nM) were determined. The binding mechanism of puerarin, which has a high inhibitory effect on the active site of the enzyme, was demonstrated by the molecular docking method. The antioxidant activity was evaluated for the same molecule. For this purpose, Fe3+-Fe2+ reducing capacity, ABTS radical scavenging activity, CUPRAC method, DPPH⋅ scavenging activity and DMPD radical scavenging method were used. The percentage (%) inhibition amount of puerarin molecule was calculated as 99.33 % for ABTS radical scavenging activity, which is the more preferred method for natural products. The absorbtion value of iron reduction of Puerarin was 0.122±0.010, and the value of copper reduction was 0.633±0.026. Our research efforts include uncovering the potent antioxidant potential of puerarin and, simultaneously, a comparative study of tacrine, primarily emphasizing its inhibitory effects against AChE and BChE enzymes. The puerarin is expected to be a guide for drug discovery against many problems, especially oxidative cell damage in Alzheimer's disease, mitochondrial dysfunction (MD), cancer, and cardiovascular diseases.
AtıfScopus11WoS5OpenAlex9İncele - MakaleAçık Erişim2023Scopus SJRQ3Chemical Content of Volatile Oil of Primula veris subsp. columnae, Obtaining the Methanol Extracts and their Biological Activities
Karapınar, Çağrı; Öz, Mehmet
The proportions and constituents of the essential oils of flowers and leaves of Primula veris subsp. columnae were determined, and the antioxidant and the antimicrobial (antibacterial and antifungal) properties of their methanol extracts were investigated. Percentage ratios and main components of the Primula veris subsp. columnae plant, which grows naturally, were detected by extracting flower and leaf volatile oils. The components of the volatile oil were identified with Gas Chromatography Mass Detector-Flame Ionization Detector (GC-MS/FID). A total of 62 compounds were identified in flower volatile oil, and methyl 4methoxysalicylate (37.1%) was determined as the main compound. While defining the structure of 50 compounds in leaf essential oil, linoleic acid (40.1%) was established as the main compound. As the result of the extraction of flowers and leaves with methanol, the extractive substance was obtained as 34.5% in flowers and as 28.8% in leaves. Methanol extraction and the antioxidant properties of the Primula veris subsp. columnae plant were quite high. In the antifungal and antibacterial activity test conducted on the volatile oils and methanol extracts of flowers and leaves of the Primula veris subsp. columnae species, only flower volatile oil showed weak inhibition properties.
AtıfScopus4WoS4OpenAlex5İncele - MakaleAçık Erişim2023Scopus SJRQ1Phenolic Constituents, Antioxidant and Antimicrobial Activity and Clustering Analysis of Propolis Samples Based on PCA from Different Regions of Anatolia
Altuntaş, Ümit; Güzel, İsmail; Özçelik, Beraat
This study aimed to evaluate the biochemical composition and biological activity of propolis samples from different regions of Türkiye to characterize and classify 24 Anatolian propolis samples according to their geographical origin. Chemometric techniques, namely, principal component analysis (PCA) and a hierarchical clustering algorithm (HCA), were applied for the first time to all data, including antioxidant capacity, individual phenolic constituents, and the antimicrobial activity of propolis to reveal the possible clustering of Anatolian propolis samples according to their geographical origin. As a result, the total phenolic content (TPC) of the propolis samples varied from 16.73 to 125.83 mg gallic acid equivalent per gram (GAE/g) sample, while the number of total flavonoids varied from 57.98 to 327.38 mg quercetin equivalent per gram (QE/g) sample. The identified constituents of propolis were phenolic/aromatic acids (chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, and trans-cinnamic acid), phenolic aldehyde (vanillin), and flavonoids (pinocembrin, kaempferol, pinobanksin, and apigenin). This study has shown that the application of the PCA chemometric method to the biochemical composition and biological activity of propolis allows for the successful clustering of Anatolian propolis samples from different regions of Türkiye, except for samples from the Black Sea region.
AtıfScopus47WoS44OpenAlex54İncele - MakaleYalnız künye2023Scopus SJRQ2LC-HRMS Profiling and Phenolic Content, Cholinesterase, and Antioxidant Activities of Terminalia citrina
Özler, Ebubekir; Topal, Fevzi; Topal, Meryem; Öztürk Sarıkaya, Sevim Beyza
Terminalia citrina (T. citrina) belongs to the Combretaceae family and is included in the class of medicinal plants in tropical countries such as Bangladesh, Myanmar, and India. The antioxidant activities of lyophilized water (WTE) and alcohol extracts (ETE) of T. citrina fruits, their phenolic content by LC-HRMS, and their effects on cholinesterases (ChEs; AChE, acetylcholinesterase, and BChE, butyrylcholinesterase) were investigated. Especially ten different analytical methods were applied to determine the antioxidant capacity. Compared with similar studies for natural products in the literature, it was determined that both WTE and ETE exhibited strong antioxidant capacity. Syringe and ellagic acids were higher than other acids in ETE and WTE. IC50 values for ETE and WTE in DPPH radical and ABTS⋅+ scavenging activities were calculated as 1.69–1.68 μg mL−1 and 6.79–5.78 μg mL−1, respectively. The results of the biological investigations showed that ETE and WTE had an inhibition effect against ChEs, with IC50 values of 94.87 and 130.90 mg mL−1 for AChE and 262.55 and 279.70 mg mL−1 for BChE, respectively. These findings indicate that with the prominence of herbal treatments, T. citrina plant may guide the literature in treating Alzheimer's Disease, preventing oxidative damage, and mitochondrial dysfunction.
AtıfScopus13WoS8OpenAlex14İncele - Yüksek Lisans TeziAçık Erişim2022Linum mucronatum subsp. Bertol armenum (Bordz.) P.H.Davis bitkisinin fenolik içeriğinin, antioksidan ve α-glukozidaz enzim inhibisyon aktivitelerinin araştırılması
Fatma Kılıç
Bitkiler özellikle fenoller, flavonoidler ve karotenoidler gibi bileşenleri içermelerinden dolayı, yüksek antioksidan aktivite gösterme potansiyeline sahiptirler. İnsanlarda oksidatif stres kaynaklı hastalıkların engellenmesinde ve tedavisinde, antioksidan savunma mekanizmaları önemli bir yere sahiptir. Bu çalışmada, Gümüşhane ilinde yayılış gösteren Linum mucronatum subsp. armenum bitkisinin yaprak ve çiçek kısımları farklı polariteye sahip beş çözücü kullanılarak ekstrakte edilmiştir. Bu ekstraktların antioksidan aktiviteleri, altı farklı antioksidan aktivite tayin yöntemi (DPPH Radikal Temizleme, Demir (III) İndirgeme/Antioksidan Güç (FRAP), Bakır (II) İndirgeyici Antioksidan Aktivite (CUPRAC), ABTS Radikal Temizleme, Toplam Fenolik İçerik ve Toplam Flavonoid İçerik) ile belirlenmiştir. DPPH, FRAP ve CUPRAC testlerinde en yüksek antioksidan aktiviteyi bitkinin yaprak kısmının etanol ekstraktı gösterirken, çiçek kısmında ise en yüksek aktiviteyi metanol ekstraktı göstermiştir. ABTS testinde ise hem çiçek (SC50=0.287 mg/ml) hem de yaprakta (SC50=0.163 mg/ml) en yüksek aktivite aseton ekstraktında ölçülmüştür. Bütün testlerde en düşük aktiviteyi hekzan ekstraktı göstermiştir. Ek olarak, LC-MS/MS analizi ile bitkide tespit edilen fenolik bileşikler protekatekuik asit, kafeik asit, vanilin, p-kumarik asit, salisilik asit, p-OH benzoik asit, rutin, kuarsetin ve kaemferoldür. Bitkinin yaprak ve çiçek kısımları α-glukozidaz enzim inhibisyon etkisi göstermiştir. Bitkinin yaprak kısmının (IC50=4.533 mg/ml) çiçekten (IC50=6.096 mg/ml) daha yüksek enzim inhibisyonu gösterdiği tespit edilmiştir. Sonuç olarak, Gümüşhane'de doğal yayılışı olan bu alttürün biyolojik aktivite gösterdiği belirlenmiştir. Elde edilen sonuçlar bitkinin biyolojik aktivitesi üzerine yapılacak çalışmalara katkı sağlayacaktır.
- Yüksek Lisans TeziAçık Erişim2022Puerarin bileşiğinin in vitro şartlarda antioksidan kapasitesinin ve bazı metabolik enzimler üzerine aktivitesinin belirlenmesi
Aslı İnan Ergün
Tez çalışmasında fenolik mokekül yapısına sahip puerarin bileşiğinin asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) enzimleri üzerine inhibisyon etkileri incelendi. AChE enzimi için elde edilen IC50 değeri 177.73 nM iken BChE için 315.07 nM olarak hesaplandı. Standart molekül olarak takrin kullanıldı. Takrin molekülünden daha etkili inhibisyon gösterdiği belirlendi. Tezin diğer kısmında ise aynı molekül için antioksidan aktivitesini değerlendirildi. Bu amaçla, Fe3+-Fe2+ indirgeme kapasitesi tayini, Azino-bis (3-etilbenzo-tiyazolin-6-sülfonik asit) (ABTS) radikali giderme aktivitesi, Cu2+-Cu+ indirgeme kapasitesi (CUPRAC metodu), 2,2- 1,1-Difenil 2-pikril hidrazil (DPPH) serbest radikalleri giderme aktivitesi, DMPD radikali giderme aktivitesi yöntemleri kullanıldı. Referans antioksidanlar olarak BHT, BHA, askorbik asit (C vitamini), troloks ve α-tokoferol (E vitamini) kullanılarak karşılaştırmaları yapıldı. ABTS radikal giderme aktivitesi için Puerarin molekülünün 20 mg/mL konsantrasyonunda % inhibisyon miktarı %99.33 olarak hesaplandı. İndirgeme kapasitelerininde standartlarla paralel olduğu belirlendi. Elde edilen sonuçların kozmetik, ilaç ve biyokimyasal yeni çalışmalara ışık tutacağı düşünülmektedir.
- Yüksek Lisans TeziAçık Erişim2022Robinia pseudoacacia ve Robinia hispida bitki özütlerinin biyolojik aktivitelerinin, fenolik ve mineral bileşimlerinin araştırılması
Betül Kara
Diyabet hastalığı dünyada en sık rastlanılan hastalıklar arasındadır. Organizmada oksidan ve anti-oksidan sistemler arasındaki dengenin oksidanlar lehine bozulmasıyla oluşan oksidatif stres diyabet hastalığının nedenleri arasındadır. Bununla birlikte, karbonhidrat yıkım mekanizmasında görevli olan α-amilaz ve α-glukozidaz enzimlerinde oluşan değişiklikler de diyabete yol açar. Bu çalışmada, halk arasında beyaz akasya ve pembe akasya olarak bilinen Robinia pseudoacacia ve Robinia hispida bitkilerinin farklı çözücü ve konsantrasyonlarda hazırlanan toprak üstü kısımlarına ait özütleri kullanılmıştır. Bu özütlerde TPC, TFC, DPPH ve ABTS, FRAP, CUPRAC metotları ile antioksidan aktiviteleri ve α-glukozidaz inhibisyonu metodu ile antidiyabetik aktiviteleri belirlenmiştir. Kromatografik LC-MS/MS analizi ile bitkilerdeki fenolik bileşikler ve miktarları karşılaştırılmıştır. ICP-MS analizi ile mineral içeriği belirlenmiştir. Ayrıca bitki özütlerinin renk analizi yapılmıştır. Bitki özütlerinin içeriği fenolik ve flavonoid madde açısından farklılık göstermekle birlikte, çözücü tipi ve konsantrasyonunun içeriğin belirlenmesinde önemli bir etken olduğu görülmüştür. İki bitki türü karşılaştırıldığında ise pembe akasya çiçek ve yapraklarına ait özütler TPC, TFC, FRAP, CUPRAC, ABTS ve DPPH açısından daha iyi aktivite sergilemiştir. Enzim inhibisyonu çalışmasında en yüksek aktiviteyi pembe akasya çiçek özütleri göstermiştir. Mineral analizi sonuçlarında özütlerde en yüksek miktarda bulunan element potasyum olarak bulunmuştur. Biyolojik olarak aktif özütlerin tanımlanması, temel biyolojik bilimler, farmasötik uygulamalar ve gelecekteki araştırmalar için çok önemlidir.
- Yüksek Lisans TeziAçık Erişim2022Gümüşhane yöresi juniperus excelsa M. Bieb. (boylu ardıç) bitki kısımlarından elde edilen uçucu yağların kimyasal içeriği ve biyolojik aktiviteleri
Emrah Saruhan
Bu çalışma Juniperus excelsa M. Bieb. bitki kısımlarından elde edilen uçucu yağının kimyasal içeriği ile antioksidan, antifungal ve antimikrobiyal özelliklerinin belirlenmesi amacıyla yapılmıştır. Çalışmada, Gümüşhane ilinde doğal olarak yetişen Juniperus excelsa M. Bieb. bitkisinin kozalak, yaprak ve dal odunlarının uçucu yağları elde edilerek ve uçucu yağların yüzde oranları ve kimyasal bileşenleri belirlenmiştir. Elde edilen uçucu yağların antioksidan aktivite tayinleri, DPPH serbest radikal temizleme aktivitesi tayini, Demir (III) iyonu indirgeyici antioksidan gücü (FRAP) tayini ve ABTS●+ radikal katyonu giderme aktivitesi yöntemine göre yapılmıştır. Ayrıca toplam antioksidan madde içeriği analizi, toplam flavonoid madde tayini ve toplam fenolik madde tayini analizleri yapılmıştır. Uçucu yağların antimikrobiyal aktivite testleri ise disk difüzyon yöntemi ile 23 farklı mikroorganizmaya karşı belirlenmiştir. Juniperus excelsa M. Bieb. türünün uçucu yağ analiz sonucunda yüzde olarak uçucu yağ verimi kozalak, yaprak ve dallardaki uçucu yağ oranları sırasıyla; % 5.88, % 2.00 ve % 0.62 olarak belirlenmiştir. Kozalak, yaprak ve dal uçucu yağlarında en fazla bulunan ana bileşik α-pinen ve yağ analizleri sonucunda % miktar olarak en fazla bulunan kimyasal sınıf, monoterpenler olmuştur. Juniperus excelsa M. Bieb. bitkisinin genel olarak uçucu yağların antioksidan aktivite tayini sonuçları değerlendirildiğinde antioksidan özelliklerinin oldukça yüksek olduğu tespit edilmiştir. Juniperus excelsa M. Bieb. türünün kozalak, yaprak ve dal uçucu yağları üzerinde yapılan antimikrobiyal aktivite testinde kozalakların, yaprakların ve dalların bakteri, maya ve küflere karşı önemli antimikrobiyal etki gösterdiği belirlenmiştir.
- MakaleAçık Erişim2022Scopus SJRQ2Effect of blueberry addition on antioxidant activity, textural, microbiological and physicochemical properties of strained yoghurt
Şengül, Mustafa; Can, Büşra; Ürkek, Bayram; Gürbüz-Kaçan, Zeynep
In this research, the microbiological, physicochemical, rheological, textural, sensory properties and antioxidant activity of blueberry pulp added (0, 4%, 8% and 12%) strained yoghurt samples were investigated during a 28 day storage period. The Lactobacillus delbrueckii spp. bulgaricus and Streptococcus thermophilus counts in the samples were found to have decreased at the end of the storage period. The lightness, yellowness/blueness and whiteness index values showed a decrease depending on the addition of blueberry, while the redness/greenness values increased. The addition of blueberry had a negative the effects on the fat and protein values, while it had a positive effect on the total solids values. The storage period did not significantly change any of the physicochemical, colorimetric or rheological properties of the strained yoghurt samples. The general acceptability scores of the sample containing 12% blueberry were higher than the other samples. The antioxidant activities and total phenolic content of the strained yoghurt samples increased in accordance with blueberry concentration, while the firmness and work of the shear values decreased. In conclusion, it was determined that the addition of blueberry pulp at a 12% ratio could be used to enhance the nutritional and functional properties of strained yoghurts.
AtıfScopus10WoS9OpenAlex8İncele - MakaleYalnız künye2022Scopus SJRQ3Chemical composition, antioxidant and antimicrobial activity of Colchicum speciosum Steven growing in Türkiye
Baltacı, Cemalettin; Öz, Mehmet; Fidan, Muhammed Said; Üçüncü, Osman; Karataş, Şeyda Merve
In the current study, the chemical constituents, antioxidant and antimicrobial activity of the volatile oil obtained from flowers and stems of C. speciosum growing in Türkiye were investigated. Both volatile oils were extracted through the hydrodistillation method in a Clevenger apparatus followed by the GC-MS analysis. The volatile oil yield attained from flowers and stems parts were determined as 0.62% and 0.65%, respectively. The most abundant compounds were determined in the flowers. Tricosane, octadecanal and hexadecanoic acid were identified to be the major components. Tricosane (38.28%) was defined to be the major component in flowers. 2-hydroxy benzaldehyde, tricosane and hexadecanoic acid were found to be the main compounds in the volatile oil of stems. 2-hydroxy benzaldehyde (46.49%) was found to be the main constituents in stems. According to the results antioxidant activity was attained as total antioxidant substance amount (86.77 mg AA/100 mL), total phenolic substance amount (65.64 mg GA/100 mL), total flavonoid substance amount (3.92 mg QE/100 mL), ferric (III) ion reducing antioxidant power capacity (6.88 mg FeSO4/100 mL) and free radical scavenging capacity (ABTS) (50.67 mg AA/100 mL) were obtained the highest antioxidant properties were obtained in flowers of C. speciosum. C. speciosum volatile oils had antimicrobial activity against Bacillus cereus in the flowers and had no activity against bacteria and yeast-mould in the stems.
AtıfScopus12İncele - MakaleAçık Erişim2022Scopus SJRQ1Evaluation of the in vitro antioxidant, antidiabetic and anticholinergic properties of rosmarinic acid from rosemary (Rosmarinus officinalis L.)
Topal, Meryem; Gulcin, İlhami
In this study, antidiabetic, anticholinergic, and antioxidant abilities of rosmarinic acid were evaluated and compared to standards. To determine its antioxidant activity different in vitro bioanalytical methods, including total antioxidant activity (TAA), ABTS•+ scavenging activity, O2•- scavenging activity, DPPH· scavenging activity, DMPD•+ scavenging activity, potassium ferric cyanide reduction ability, cupric ion (Cu2+) reduction capacity, H2O2 scavenging activity, and ferrous ions (Fe2+) binding activities were separately performed. Also, butylated hydroxyanisole (BHA), Trolox, α-tocopherol, and butylated hydroxytoluene (BHT) were used as the reference antioxidants. Rosmarinic acid inhibited the linoleic acid emulsion peroxidation by 89.2% while BHA, BHT, Trolox and α-tocopherol inhibited as 87.3, 97.6, 90.3 and 75.3%, respectively, at the same concentration (30 μg/mL). When these results are evaluated, rosmarinic acid had effective TAA than BHA and α-tocopherol and close to BHT and Trolox. In addition, rosmarinic acid exhibited Kİ values of 9.67 ± 0.81 nM towards acetylcholinesterase (AChE) and 33.56 ± 2.96 nM against α-glycosidase enzymes.
AtıfScopus94WoS81OpenAlex85İncele - MakaleAçık Erişim2021Scopus SJRQ1Chemical compositions by using LC-MS/MS and GC-MS and antioxidant activities of methanolic extracts from leaf and flower parts of Scabiosa columbaria subsp. columbaria var. columbaria L.
Akar, Zeynep
The members of the Scabiosa genus are one of the traditional medicinal plants used in the treatment of many diseases, in particular the treatment of scabies. In this study, it was aimed to determine antioxidant activities and chemical composition of methanolic extracts of leaves and flowers of Scabiosa columbaria subsp. columbaria var. columbaria. The phenolic contents of both parts of the plant were analyzed by LC-MS/MS. A total of 6 phenolic compounds were determined and chlorogenic acid was the major compound in both flower and leaf parts of the plants, with 5936.052 mg/g and 8021.666 mg/g, respectively. 6 different methods were used to determine the antioxidant activity of the plant parts. Both leaf and flower parts of the plant showed high antioxidant activity in all tested methods and the antioxidant activity values of the leaf part were measured higher than those of the flower part for four tests. The methanol extracts of the plant parts was analyzed with GC-MS and number of the essential oil compounds in the leaf and flower parts were determined as 17 and 13, respectively. Linalool compound was also found to be common in both parts of the plant. The major compounds of the essential oils were identified as 4Octadecenal (30.01%) in the flower and carvone (35.44%) in the leaf. In addition, terpene derivatives was determined as 90.32% of the highest essential oil group in the leaf, while this value was determined as 1.42% in the flower. For the flower, aromatics were determined as the main component group with 21.31%. (c) 2021 The Author. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
AtıfWoS22OpenAlex29İncele