Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2023An Investigation of the Protective Effect of Rhododendron luteum Extract on Cisplatin-Induced DNA Damage and Nephrotoxicity and Biochemical Parameters in Rats
Turan, Ibrahim; Canbolat, Deniz; Demir, Selim; Kerimoglu, Gokcen; Colak, Fatih; Alemdar, Nihal Turkmen; Mentese, Ahmet; Aliyazicioglu, Yuksel
The nephrotoxicity mechanisms caused by cisplatin (CP), which is used in the treatment of different types of cancer, include several processes, such as DNA damage, apoptosis, inflammation, and oxidative stress. This study investigated the protective effects of Rhododendron luteum leaf extract (RLE) in a rat model of CP -induced nephrotoxicity. Dimethyl sulfoxide extract of RLE was prepared, and its contents were clarified using liquid chromatography-mass spectrometry (LC-MS -MS). Thirty female Wistar Albino rats were divided into five different groups and sacrificed on the seventh day. Biochemical and histopathological analyses were then performed. The presence of quercitrin, quinic acid, chlorogenic acid, protocatechic acid, isoquercitrin, and quercetin was detected in the RLE. While CP significantly increased the levels of oxidative stress, inflammation, and apoptosis markers in the rat kidney tissue and blood samples, treatment with RLE significantly reversed the levels of these parameters in a dose-dependent manner. Total antioxidant status (TAS) values and glutathione (GSH) levels increased significantly in the serum samples from the treatment group compared to the CP group, while blood urea nitrogen (BUN), creatinine, oxidative stress index (OSI), tumor necrosis factor-alpha (TNF-alpha), malondialdehyde (MDA) and total oxidant status (TOS) values decreased significantly. GSH levels increased significantly in the treatment group compared to the CP group, while TNF-alpha, caspase-3 (Casp-3), 8-hydroxy-2'-deoxyguanosine (8-OHdG), OSI, TOS, MDA levels, and damage scores decreased significantly at histopathological examination. In conclusion, RLE exhibited strong protective effects against nephrotoxicity and oxidative damage caused by CP in rats.
- MakaleYalnız künye2022An Investigation of the Antiproliferative Effect of Rhododendron luteum Extract on Cervical Cancer (HeLa) Cells via Nrf2 Signaling Pathway
Turan, Ibrahim; Demir, Selim; Yaman, Serap Ozer; Canbolat, Deniz; Mentese, Ahmet; Aliyazicioglu, Yuksel
The aim of the present study was to investigate the role of Rhododendron luteum extract (RLE) in the induction of Nrf2‑related oxidative stress and endoplasmic reticulum (ER) stress in human cervical cancer (HeLa) cells. The antiproliferative effect of RLE on HeLa and fibroblast cells was determined using the MTT assay. The effects of RLE on the cell cycle, apoptosis, and production of reactive oxygen species (ROS) in HeLa cells were evaluated using fluorescent probes. The mRNA expression levels of Nrf2 [and its targets glutamate-cysteine ligase catalytic subunit (GCLC), and glucose-6-phosphate dehydrogenase (G6PD)], and C/EBP homologous protein (CHOP, an ER stress marker were determined using reverse transcription‑quantitative polymerase chain reaction (RT-PCR). The results demonstrated that RLE exhibited a selective cytotoxic effect (2.9-fold) on HeLa cells compared to fibroblast cells. RLE arrested the cell cycle at the S phase, and induced apoptosis, ER stress, and ROS formation. In addition, RLE significantly suppressed the expression levels of Nrf2, GCLC and G6PD (0.65, 0.69, and 0.54-fold, respectively) and increased the expression of CHOP (4.48-fold) in HeLa cells at 72 h of treatment (p < 0.05). These results show that the antiproliferative effect of RLE occurs through the Nrf2 and ER stress pathways, and the results should now be supported by further in vivo studies.
- MakaleAçık Erişim2021Investigation the Different Extraction Temperature Effect of Artvin Savsat Region Propolis PBS Extracts on Antioxidant Properties and Eryhrocyte Hemolysis Inhibition
Canbolat, Deniz; Turan, Ibrahim; Küpeli, Yunus Emre; Kilinç, Sedanur; Piliç, Sevim
Propolis, which has been used in traditional medicine for hundreds of years all over the world, is a resinous natural product with a complex content obtained by honey bees by mixing the buds of plants and pollen grains with enzymes they secrete. Propolis may have different contents due to the gathering region, plant source, bee variety and climate of the place where it is collected. Although there are many studies related to different regions, so far the propolis of the Savsat region in Artvin have not been studied yet. The aim of this study is to determine the antioxidant properties (determination of iron reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic and total flavonoid content) of propolis extracts of Artvin Savsat region prepared with phosphate buffer salt solution (PBS) prepared at different temperatures (45 and 60 degrees C) and to determine the erythrocyte hemolysis inhibition properties of the extracts. Total phenolic (11.91 +/- 0.5-24.14 +/- 1.38 mg gallic acid equivalent to g propolis) and total flavonoid (1.79 +/- 0.03-2.13 +/- 0.07 mg quercetin equivalent to g propolis) content of PBS extracts, iron power determination (12.80 +/- 0.36-27.15 +/- 0.12 mg ascorbic acid equivalent to g propolis), 2,2-diphenyl-1-picrilhydrazyl (DPPH) radical scavenging activity (%22.86 +/- 0.5-31.24 +/- 1.33) and erythrocyte hemolysis inhibition (%55.58 +/- 0.57-96.37 +/- 0.02) were determined using spectrophotometric methods. As a result of the experiments, it was determined that the extract with PBS obtained at a temperature of 60 degrees C was higher than the extract obtained at 45 degrees C, in terms of antioxidant properties and erythrocyte hemolysis inhibition ratio. Further studies are required to identify molecules that inhibit erythrocyte hemolysis in extracts and have antioxidant properties.
- MakaleAçık Erişim2021The Effect of Rosa pimpinellifolia Extract on the Proliferation of Human Tumor Cells
Demir, Elif Ayazoglu; Demir, Selim; Türkmen, Nihal; Turan, Ibrahim
The aim of this study was to evaluate the antioxidant properties of R. pimpinellifolia and its cytotoxic effect against human colon (WiDr), liver (HepG2) and lung (A549) cancer cells compared with a normal fibroblast (BJ) cell line. The antioxidant properties of extract was determined using spectrophotometric methods, while the cytotoxic effect of extract was evaluated using MTT assay. The total phenolic content, total flavonoid content and reducing power values of extract were calculated as 16.4 +/- 0.4 mg gallic acid equivalent, 5.2 +/- 0.2 mg quercetin equivalent and 34.3 +/- 2.4 mg trolox equivalent per g sample, respectively. Although the extract exhibits selective cytotoxic effect on three cancer cell lines in a dose-dependent manner compared to BJ cells, the highest selectivity index value (2.7-fold) was obtained for the WiDr cell line. In conclusion, this is the first study to demonstrate the cytotoxic effect of R. pimpinellifolia on cancer cells. To demonstrate the molecular mechanism of this cytotoxic effect and to carry out in vivo studies may be a guide for future clinical studies.
- MakaleAçık Erişim2021Evaluation of Cytotoxic Effect of Onosma armeniacum Extract on Various Cancer Cells
Demir, Selim; Ayazoglu Demir, Elif; Turan, Ibrahim; Özgen, Ufuk
The aim of this study was to investigate the antioxidant properties of the root extract of Onosma armeniacum and its cytotoxic effect against human liver (HepG2), lung (A549) and colon (WiDr) cancer cell lines compared with a normal fibroblast cell line. Antioxidant properties of the extract was determined using the colorimetric methods. The cytotoxic effect of the extract were investigated using MTT assay. The total phenolic content and reducing power values of extract were 26.1 +/- 0.4 mg gallic acid equivalent and 52.6 +/- 1.1 mg trolox equivalent per g sample, respectively. The extract exhibited selective cytotoxic effect in all studied cancer cell lines, especially HepG2 (3.6-fold). The results of this study reveal that O. armeniacum, which has been found to have acceptable antioxidant and cytotoxic effects, should be examined with more comprehensive studies.
- MakaleAçık Erişim2021Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines
Ayazoglu Demir, Elif; Demir, Selim; Turan, Ibrahim
Ethyl pyruvate (EP) is a simple aliphatic ester derived from pyruvic acid which is an endogenous metabolite. Although various studies have investigated the antioxidant and anti-inflammatory properties of EP, there has been only limited research into the cytotoxic effect of EP on cancer cells. The aim of this study was to determine the cytotoxic effects of EP on cells representing common cancer types. EP was purchased commercially and intermediate stock solutions were prepared with phosphate buffer saline. The cytotoxic effect of EP on human melanoma (VMM917), cervix (HeLa), breast (MCF-7), lung (A549), liver (HepG2), colon (WiDr) cancer and normal fibroblast (BJ) cells was determined using the MTT assay. Cisplatin was used as a positive control in cytotoxicity experiments. The results showed that EP exhibits selective cytotoxic effect on VMM917 (10.1-fold) and HeLa (3.04-fold) cells compared to BJ cells. This study shows for the first time that EP has a highly selective cytotoxic effect, especially on melanoma and cervix cancer cells. The mechanism of this effect needs to be elucidated by more extensive studies.
- MakaleYalnız künye2021The effect of homocysteine on the expression of CD36, PPARγ, and C/EBPα in adipose tissue of normal and obese mice
Mentese, Ahmet; Dogramaci, Seniz; Demir, Selim; Yaman, Serap Ozer; Ince, Imran; Altay, Diler Us; Erdem, Mehmet; Turan, Ibrahim; Alver, Ahmet
The purpose of this study was to investigate the effect of homocysteine (Hcy) on CD36, PPARγ, and C/EBPα gene and protein expression in adipose tissue obtained from normal and high-calorie diet obesity models. CD36, PPARγ, and C/EBPα gene expression and protein levels in adipose tissue specimens were determined using the RT-PCR and ELISA methods, respectively. Significantly increased CD36 gene expression was observed in adipose tissue from obese mice, while Hcy significantly reduced CD36 gene expression in adipose tissue from normal and obese mice. PPARγ and C/EBPα gene expression levels decreased significantly in all groups compared to the normal group. In addition, levels of both PPARγ and C/EBPα gene expression were lower with Hcy supplementation compared to their own controls. In conclusion, Hcy’s reduction of CD36 gene expression in adipose tissue may be one probable factor in hyperhomocysteinemia representing an independent risk factor for cardiovascular diseases.
- MakaleYalnız künye2020Effect of Turkish Propolis on miRNA Expression, Cell Cycle, and Apoptosis in Human Breast Cancer (MCF-7) Cells
Misir, Sema; Aliyazicioglu, Yüksel; Demir, Selim; Turan, Ibrahim; Hepokur, Ceylan
Enriched in flavonoid compounds, phenol acids, and terpene derivatives, propolis has been shown to regulate apoptosis signaling pathways and alter the expression of microRNAs (miRNAs). In the present study, it has been aimed to examine the effects of Turkish propolis on miRNA levels of breast cancer (MCF-7) cells, and its relationship with cell proliferation and apoptosis. Cytotoxic activity of ethanolic propolis extract (EEP) was evaluated using MTT assay. Mechanisms involved in the cytotoxic action of Turkish propolis in MCF-7 cells were investigated with regard to apoptosis and cell cycle using flow cytometry and western blot. Mitochondrial membrane potential (MMP) were evaluated by spectrofluorometric method. miRNA levels were detected by qRT-PCR method. EEP exhibited selective toxicity against MCF-7 cells compared to normal fibroblast cells. EEP increased the cell cycle arrest at the G1 phase. EEP elevated the apoptotic cell death through increasing pro-apoptotic protein levels (p21, Bax, p53, p53-Ser46, and p53-Ser15), decreasing MMP and altering the expression levels of specific tumor suppressors (miR-34, miR-15a, and miR-16-5p) and oncogenic (miR-21) miRNAs. These data support that Turkish propolis may be evaluated as a potential natural agent for new anticancer drugs in future.
- MakaleYalnız künye2020Rosa canina Extract has Antiproliferative and Proapoptotic Effects on Human Lung and Prostate Cancer Cells
Kilinc, Kagan; Demir, Selim; Turan, Ibrahim; Mentese, Ahmet; Orem, Asim; Sonmez, Mehmet; Aliyazicioglu, Yuksel
Although several studies have investigated the cytotoxic effects of different Rosa species, there has been only limited research into the cytotoxic effect of Rosa canina. The purpose of this research was to evaluate the antioxidant properties, phenolic characterization, and cytotoxic effects of R. canina on human lung (A549) and prostate (PC-3) cancer cells and the possible mechanisms involved. The antioxidant properties and phenolic characterization of the extract were determined using spectrophotometric methods and RP-HPLC, respectively. The cytotoxic activity of the extract was determined using the MTT assay. The mechanism involved in the extract’s cytotoxic effect was then evaluated in terms of apoptosis, the cell cycle, mitochondrial membrane potential (MMP), and caspase activity using fluorometric and luminometric methods. The TPC value of the extract was 58.97 ± 2.22 mg gallic acid equivalents per gram sample, and ascorbic acid and p-coumaric acid were detected as major phenolics in the extract. R. canina extract exhibited a selective cytotoxic effect on A549 and PC-3 cells compared to normal fibroblast cells. The extract induced cell cycle arrest at the G1 phase and apoptosis via reduced MMP and increased caspase activity in these cells. Phytomedical applications of R. canina may represent promising approaches in the treatment of cancer.
- MakaleAçık Erişim2019Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells
Demir, Selim; Turan, Ibrahim; Misir, Sema; Aliyazicioglu, Yuksel
Although some studies have evaluated the cytotoxic activity of different Fabaceae species, there has been only limited research into the cytotoxic effect of Dorycnium pentaphyllum. The purpose of this study was to determine the antioxidant properties and cytotoxic effect of D. pentaphyllum extract on various human cancer cells. The total phenolic content (TPC) of the extract was determined using colorimetric method. The cytotoxic effect of the extract on human lung (A549), liver (HepG2), and breast (MCF-7) cancer cells and a normal human fibroblast cells was assessed using the MTT assay. TPC value of extract was found as 140.3 +/- 1.1 mg gallic acid equivalent per g sample. Extract showed selective cytotoxicity on all studied cancer cells compared to normal fibroblast cells, and the IC50 values of the extract in the cancer cells range from 100.4 to 298.5 mu g/mL. This is the first study to reveal the cytotoxic effect of D. pentaphyllum extract on cancer cell lines. Phytomedical applications of D. pentaphyllum may represent promising approaches in the treatment of cancer.
- MakaleAçık Erişim2019Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells
Aliyazicioglu, Yuksel; Demir, Selim; Yaman, Scrap Ozer; Sener, Sila Ozlem; Demir, Elif Ayazoglu; Aliyazicioglu, Rezzan; Turan, Ibrahim
The purpose of this study was to investigate the phytochemical analysis of D. pentaphyllum and its cytotoxic activity on human cervical cancer (HeLa) cells with possible mechanisms. Total phenolic contents (TPC) and phytochemical analysis of the extract were evaluated using spectrophotometric method and RP-HPLC, respectively. The cytotoxic effect of the extract was evaluated using the MTT assay. The mechanism of the cytotoxic effect of the extract was also evaluated in terms of apoptosis, cell cycle, and mitochondrial membrane potential (MMP) using the fluorometric methods. The TPC of the extract was calculated as 68.9 +/- 2.3 mg gallic acid equivalent per g sample and quercetin was determined as the major phenolic in the extract. The extract showed a selective cytotoxic effect (6.5-fold) on HeLa cells compared to normal fibroblast cells. The cytotoxic effect of the extract was found to be due to the arresting of the cell cycle in the S phase and increasing apoptosis through decreased MMP. Further studies are needed for the comprehensive effects of the extract on cell signaling.
- MakaleAçık Erişim2019Antioxidant Properties of Primula vulgaris Flower Extract and Its Cytotoxic Effect on Human Cancer Cell Lines
Demir, Selim; Turan, Ibrahim; Aliyazicioglu, Yuksel
Primula vulgaris is a plant belonging to Primula genus, and these species are often used in traditional therapy. Although there are numerous studies on the antioxidant and cytotoxic effects of different Primula species, there are limited studies of these properties of P. vulgaris extracts. The aim of this study was to determine the antioxidant properties and cytotoxic effects of the extract prepared with dimethyl sulfoxide from P. vulgaris flowers. The total phenolic content, total flavonoid content, and reducing antioxidant power of the extract were determined using spectrophotometric methods. The cytotoxic effect of the extract on human colon (WiDr), lung (A549), liver (HepG2), breast (MCF-7), and prostate (PC-3) cancer cells and a normal human fibroblast cell line was assessed using the MTT assay. Total phenolic content and reducing power values of extract were found 33.02 +/- 0.92 mg gallic acid equivalents, and 64.86 +/- 1.18 mg trolox equivalents per g sample, respectively. Extract exhibited selective cytotoxic effect on all studied cancer cells compared to normal fibroblast cells, and the IC50 values of the extract in the cancer cell lines range from 191.8 to 375.3 mu g/mL. This work is one of the pioneering work that reveals the powerful antioxidant properties of P. vulgaris flower extract and its selective cytotoxic effect on cancer cell lines. Further studies are needed to identify the active molecules in the extract and the cytotoxic action mechanisms of these molecules.
- MakaleYalnız künye2019Dimethyl Sulfoxide Extract of Dianthus carmelitarum Induces S Phase Arrest and Apoptosis in Human Colon Cancer Cells
Turan, Ibrahim; Demir, Selim; Aliyazicioglu, Rezzan; Kilinc, Kagan; Ozer Yaman, Serap; Akbulut Cakiroglu, Kubra; Kanbolat, Seyda; Ayazoglu Demir, Elif; Mentese, Ahmet; Aliyazicioglu, Yuksel
Although several studies have investigated the cytotoxic effects of different Dianthus species, there has been only limited research into the cytotoxic effect of Dianthus carmelitarum. The purpose of this research was to evaluate the phenolic characterization and the cytotoxic effect of D. carmelitarum on human colon cancer (WiDr) cells and the possible mechanisms involved. Total polyphenolic contents (TPC) and phenolic characterization of the extract were evaluated using the Folin-Cioceltau method and reversed-phase high performance liquid chromatography (RP-HPLC), respectively. The cytotoxic activity of the extract was determined using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The mechanism involved in the extract’s cytotoxic effect was then evaluated in terms of apoptosis and the cell cycle using flow cytometry, while mitochondrial membrane potential (MMP) was investigated using the fluorometric method. The TPC value of the extract was 784.8 ± 40.3 mg gallic acid equivalent per 100 g sample, and sinapic acid and benzoic acid were detected as major phenolics in the extract. D. carmelitarum extract exhibited a selective cytotoxic effect (3.6-fold) on WiDr cells compared to normal colon cells. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in WiDr cells. Phytomedical and nutraceutical applications of D. carmelitarum may represent promising approaches in the treatment of cancer.
- MakaleAçık Erişim2018Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines
Demir, Selim; Turan, Ibrahim; Aliyazicioglu, Yuksel
Rhododendron luteum belongs to the genus Rhododendron, members of which are frequently used in folk medicine. Various studies have investigated the cytotoxic effect of different Rhododendron species, but there have been limited studies of the cytotoxic effect of R. luteum. The aim of this study was to investigate the antioxidant properties and cytotoxic effect of R. luteum leaf extract on the human cancer cells. The total phenolic content (TPC), total flavonoid content (TFC), and reducing power of the extract were evaluated using spectrophotometric procedures. The cytotoxic effect of the extract on five cancers (human breast, colon, lung, prostate, and liver carcinoma) and human fibroblast cells was determined using the MTT assay. TPC and reducing power values of extract were found 173.2 +/- 3.97 mg gallic acid equivalents, and 629.5 +/- 3.49 mg trolox equivalents per g sample, respectively. R. luteum leaf extract exhibited selective cytotoxicity especially against colon (1.9 fold) and liver (2.2 fold) cancer cells compared to normal fibroblast cells. This is the first study to reveal the cytotoxic effect of R. luteum leaf extract. Further studies are now needed to identify the cytotoxic molecules in the extract and their mechanisms.
- MakaleAçık Erişim2018Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression
Turan, Ibrahim; Demir, Selim; Kilinc, Kagan; Yaman, Serap Ozer; Misir, Sema; Kara, Hanife; Genc, Berna; Mentese, Ahmet; Aliyazicioglu, Yuksel; Deger, Orhan
Rosa canina is a member of the genus Rosa that has long been used for medical objectives. Several studies have reported cytotoxic effects of different Rosa species, but there has been only limited investigation of the cytotoxic effect of R. canina. The purpose of the current study was to examine the potential effect of R. canina extract on cell viability, the cell cycle, apoptosis, and the expression of telomerase in human colon cancer (WiDr) cells. The cytotoxic effect of the extract was determined using MTT assay. The mechanism involved in the cytotoxic effect of the extract was then evaluated in terms of apoptosis and the cell cycle using flow cytometry. Mitochondrial membrane potential (MMP) was investigated using the fluorometric method, and expression levels of telomerase were studied using RT-PCR. R. canina extract exhibited a selective cytotoxic effect on WiDr cells compared with normal colon cells. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in WiDr cells. R. canina extract significantly repressed telomerase expressions at treatment times of 48 and 72 h in WiDr cells. Our results suggest that R. canina may have considerable potential for development as a novel natural product-based anticancer agent.
- MakaleAçık Erişim2018Antioxidant properties and protective effect of Turkish propolis on t-BHP-induced oxidative stress in foreskin fibroblast cells
Misir, Sema; Aliyazicioglu, Yuksel; Demir, Selim; Turan, Ibrahim; Yaman, Serap Ozer; Deger, Orhan
Objective: Propolis is an important bee product, rich in polyphenolic compounds. It has antitumoral, antioxidant, antimutagenic, and other useful activities. Biological activities of propolis are generally attributed to its substance of polyphenolic compounds. The aim of this study was to investigate the in vitro antioxidant properties and reduction amount of intracellular reactive oxygen species (ROS) in human normal foreskin fibroblast cells by Turkish propolis ethanolic extract (EEP). Method: Total phenolic and flavonoid contents, reducing power, radical scavenging capacity, and iron chelating activity of EEP were determined using spectrophotometric methods. Intracellular ROS levels were determined by spectrofluorometric analysis with CM-H2 DCFDA. Results: Our results indicated that antioxidant activity was correlated with the higher total phenolic and flavonoid contents of EEP. The generated ROS levels were significantly decreased by EEP compared to only t-BHP group. Conclusion: Ethanolic propolis extract may be evaluated as a potency antioxidant resource and/or a novel natural agent in food and drug industries.
- MakaleAçık Erişim2018Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells
Demir, Selim; Turan, Ibrahim; Aliyazicioglu, Rezzan; Yaman, Serap Ozer; Aliyazicioglu, Yuksel
Primula vulgaris belongs to the genus Primula, members of which are frequently used in folk medicine. Various studies have investigated the cytotoxic effect of different Primula species, but there have been limited studies on the cytotoxic effect of P. vulgaris. The aim of this study was to investigate the cytotoxic effects, and possible mechanisms involved, of P. vulgaris flower extract on human cervical cancer (HeLa) cells. The cytotoxic effect of the extract on HeLa cells was revealed using the MTT assay. Mechanisms involved in the extract's cytotoxic effect were then investigated in terms of apoptosis, mitochondrial membrane potential, and the cell cycle, using fluorometric methods. P. vulgaris flower extract exhibited selective cytotoxic effects against HeLa cells by arresting their cell cycle at the S phase, and inducing the number of apoptotic cells compared to normal fibroblast cells by reducing mitochondrial membrane potential in a concentration-dependent manner. This is the first study to reveal the antiproliferative effect of P. vulgaris flower extract. Further studies are now needed to identify the cytotoxic molecules in the extract and their mechanisms.
- MakaleAçık Erişim2017Evaluation of Antioxidant and Cytotoxic Properties of Primula vulgaris Leaf Extract
Turan, Ibrahim; Demir, Selim; Aliyazicioglu, Rezzan; Aliyazicioglu, Yuksel
Primula vulgaris belongs to the genus Primula and this genus is frequently used in traditional medicine. Numerous studies reported the antioxidant and cytotoxic effects of various extracts of different Primula species but, there were limited studies of the antioxidant and cytotoxic effect of P. vulgaris. The aim of this study was to determine the antioxidant properties and cytotoxic effects of dimethyl sulfoxide extract of leaves of P. vulgaris. The total polyphenolic content (TPC), total flavonoid content (TFC) and reducing antioxidant power of extract were evaluated using spectrophotometric procedures. The cytotoxic effects of extract were revealed on human lung (A549), liver (HepG2), breast (MCF-7), prostate (PC-3), and colon (WiDr) cancer cell lines and normal fibroblast cells using thiazolyl blue tetrazolium bromide (MTT) assay. TPC and FRAP values were 1890.1 +/- 11.2 mg gallic acid equivalents and 3233.1 +/- 176.2 mg trolox equivalents per 100 g sample, respectively. The extract showed selective cytotoxic effects on all studied cancer cell lines compared to normal fibroblast cells and IC50 values ranged from 133.3-253.8 mu g/mL. This is the first study to reveal the antioxidant properties of P. vulgaris leaf extract and its cytotoxic effect on cancer cell lines. Further studies are now needed to identify the antioxidant and cytotoxic molecules in the extract and their mechanisms.
- MakaleAçık Erişim2017Antiproliferative and apoptotic effect of Morus nigra extract on human prostate cancer cells
Turan, Ibrahim; Demir, Selim; Kilinc, Kagan; Burnaz, Nesibe Arslan; Yaman, Serap Ozer; Akbulut, Kubra; Mentese, Ahmet; Aliyazicioglu, Yuksel; Deger, Orhan
Background: Morus nigra L. belongs to the family Moraceae and is frequently used in traditional medicine. Numerous studies have investigated the antiproliferative effects of various extracts of different Morus species, but studies involving the in vitro cytotoxic effect of M. nigra extract are very limited. The purpose of this study was to evaluate the phenolic composition and antioxidant activity of dimethyl sulfoxide extract of M. nigra (DEM) and to investigate, for the first time, the probable cytotoxic effect in human prostate adenocarcinoma (PC-3) cells together with the mechanism involved. Methods: Total polyphenolic contents (TPC), ferric reducing antioxidant power (FRAP) and phenolic compounds of DEM were evaluated using spectrophotometric procedures and HPLC. The cytotoxic effect of DEM on PC-3 cells was revealed using the MTT assay. Mechanisms involved in the cytotoxic effect of DEM on PC-3 cells were then investigated in terms of apoptosis, mitochondrial membrane potential and cell cycle using flow cytometry, while caspase activity was investigated using luminometric analysis. Results: TPC and FRAP values were 20.7 ± 0.3 mg gallic acid equivalents and 48.8 ± 1.6 mg trolox equivalents per g sample, respectively. Ascorbic acid and chlorogenic acid were the major phenolic compounds detected at HPLC analysis. DEM arrested the cell cycle of PC-3 cells at the G1 phase, induced apoptosis via increased caspase activity and reduced mitochondrial membrane potential. Conclusions: Our results indicate that M. nigra may be a novel candidate for the development of new natural product based therapeutic agents against prostate cancer.
- MakaleAçık Erişim2017Phenolic characterisation and antioxidant activity of Primula vulgaris and its antigenotoxic effect on fibroblast cells
Ozkan, Mehtap Tugce; Aliyazicioglu, Rezzan; Demir, Selim; Misir, Sema; Turan, Ibrahim; Yildirmis, Sermet; Aliyazicioglu, Yuksel
Background: Primula vulgaris has been used in traditional treatment, and its biological functions are attributed to its polyphenolics content. Objectives: The aim of this study was to determine the phenolics composition and the antioxidant activity of water extract of P. vulgaris (WEP) and to determine its probable preventive effects against H2O2-induced DNA damage in human fibroblast cells. Methods: The total polyphenolic content (TPC), ferric reducing antioxidant power (FRAP), and radical scavenging activity of WEP were determined using spectrophotometric methods. Phenolic compounds and antigenotoxic effects of WEP were evaluated using HPLC and comet assay, respectively. Results: The TPC and FRAP values of WEP were 15.023 ± 0.84 mg gallic acid and 82.63 ± 0.31 μM trolox per g sample, respectively. ρ-coumaric acid and rutin were detected as major phenolics. Moreover, WEP reducedH2O2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to the positive controls (only 20 μMH2O2 treatment). Conclusions: Primula vulgaris can be used in food, cosmetics, and drug industries because of its antioxidant and antigenotoxic activities.