Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2021Antidiabetic, anticholinergic and antioxidant activities of aerial parts of shaggy bindweed (Convulvulus betonicifolia Miller subsp.) - profiling of phenolic compounds by LC-HRMS
Bingol, Zeynebe; Kiziltas, Hatice; Gören, Ahmet C.; Kose, Leyla Polat; Topal, Meryem; Durmaz, Lokman; Alwasel, Saleh H.; Gulcin, Ilhami
In order to evaluate the antioxidant activity of evaporated ethanolic extract (EESB) and lyophilized water extract (WESB) of Shaggy bindweed (Convulvulus betonicifolia Mill. Subs), some putative antioxidant methods such as DPPH. scavenging activity, ABTS(center dot)(-) scavenging effect, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+) binding activities were separately performed. Also, ascorbic acid, alpha-tocopherol and BHT were used as the standard compounds. Additionally, some phenolic compounds that responsible for antioxidant abilities of EESB and WESB were screened by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the same concentration, EESB and WESB demonstrated effective antioxidant abilities when compared to standards. In addition, EESB demonstrated IC50 values of 1.946 mu g/mL against acetylcholinesterase (AChE), 0.815 mu g/mL against alpha-glycosidase and 0.675 mu g/mL against alpha-amylase enzymes.
- MakaleAçık Erişim2016Antioxidant, antiradical, and anticholinergic properties of cynarin purified from the Illyrian thistle (Onopordum illyricum L.)
Topal, Meryem; Gocer, Hulya; Topal, Fevzi; Kalin, Pinar; Köse, Leyla Polat; Gulçin, Ilhami; Çakmak, Kader C.; Küçük, Murat; Durmaz, Lokman; Gören, Ahmet C.
Cynarin is a derivative of hydroxycinnamic acid and it has biologically active functional groups constituent of some plants and food. We elucidated the antioxidant activity of cynarin by using different in vitro condition bioanalytical antioxidant assays like DMPD•+, ABTS•+, DPPH• and H2O2 scavenging effects, the total antioxidant influence, reducing capabilities, Fe2+ chelating and anticholinergic activities. Cynarin demonstrated 87.72% inhibition of linoleic acid lipid peroxidation at 30 g/mL concentration. Conversely, some standard antioxidants like trolox, tocopherol, butylated hydroxytoluene (BHT), and butylated hydroxyanisole (BHA) exhibited inhibitions of 90.32, 75.26, 97.61, 87.30%, and opponent peroxidation of linoleic acid emulsion at the identical concentration, seriatim. Also, cynarin exhibited effective DMPD•+, ABTS•+, DPPH•, and H2O2 scavenging effects, reducing capabilities and Fe2+ chelating effects. On the contrary, IC50 and Ki parameters of cynarin for acetylcholinesterase enzyme inhibition were determined as 243.67 nM (r2: 0.9444) and 39.34 ± 13.88 nM, respectively. This study clearly showed that cynarin had marked antioxidant, anticholinergic, reducing ability, radical-scavenging, and metal-binding activities.
- MakaleYalnız künye2011Polyphenol contents and antioxidant properties of medlar (Mespilus germanica L.)
Gülçin, Ilhami; Topal, Fevzi; Sarikaya, S. Beyza Öztürk; Bursal, Ercan; Bilsel, Gökhan; Gören, Ahmet C.
Medlar is the fruit of Mespilus germanica L. in the family of Rosaceae. The present study outlines that the native medlar (Mespilus germanica L.) fruits an extremely rich source of antioxidants. In this study, antioxidant and antiradical property of medlar fruits were evaluated. Total phenolics and flavonoids amounts in lyophilized extract of medlar (LEM) fruits were calculated as gallic acid and quercetin equivalents, respectively. Antioxidant and radical scavenging activity of LEM were investigated using different in vitro assays including 1,1-diphenyl-2-picryl-hydrazyl (DPPH·), N,N-dimethyl-p-phenylenediamine (DMPD•+), and superoxide anion radicals (O2•-) scavenging activity, hydrogen peroxide (H2O2), ferric ions (Fe 3+) and cupric ions (Cu 2+) reducing ability, Fe 3+ -TPTZ reducing ability, ferrous ions (Fe2+) chelating activity as trolox equivalent. In addition, quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, α-tocopherol, pyrogallol, p-hydroxybenzoic acid, vanillin, p-coumaric acid, gallic acid and ascorbic acid in LEM were detected by high performance liquid chromatography and tandem mass spectrometry (LC-MS/MS). The presence of these antioxidant compounds can be considered as a quality parameter for edible medlar fruits.