Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2016Antioxidant activity of taxifolin: An activity-structure relationship
Topal, Fevzi; Nar, Meryem; Gocer, Hulya; Kalin, Pinar; Kocyigit, Umit M.; Gülçin, Ilhami; Alwasel, Saleh H.
Taxifolin is a kind of flavanonol, whose biological ability. The objectives of this study were to investigate the antioxidants and antiradical activities of taxifolin by using different in vitro bioanalytical antioxidant methods including DMPD•+, ABTS•+, and DPPH•-scavenging effects, the total antioxidant influence, reducing capabilities, and Fe2+-chelating activities. Taxifolin demonstrated 81.02% inhibition of linoleic acid emulsion peroxidation at 30 μg/mL concentration. At the same concentration, standard antioxidants including trolox, α-tocopherol, BHT, and BHA exhibited inhibitions of linoleic acid emulsion as 88.57, 73.88, 94.29, and 90.12%, respectively. Also, taxifolin exhibited effective DMPD•+, ABTS•+, and DPPH•-scavenging effects, reducing capabilities, and Fe2+-chelating effects. The results obtained from this study clearly showed that taxifolin had marked antioxidant, reducing ability, radical scavenging and metal-chelating activities. Also, this study exhibits a scientific shore for the significant antioxidant activity of taxifolin and its structure-activity insight.
- MakaleAçık Erişim2016Antioxidant, antiradical, and anticholinergic properties of cynarin purified from the Illyrian thistle (Onopordum illyricum L.)
Topal, Meryem; Gocer, Hulya; Topal, Fevzi; Kalin, Pinar; Köse, Leyla Polat; Gulçin, Ilhami; Çakmak, Kader C.; Küçük, Murat; Durmaz, Lokman; Gören, Ahmet C.
Cynarin is a derivative of hydroxycinnamic acid and it has biologically active functional groups constituent of some plants and food. We elucidated the antioxidant activity of cynarin by using different in vitro condition bioanalytical antioxidant assays like DMPD•+, ABTS•+, DPPH• and H2O2 scavenging effects, the total antioxidant influence, reducing capabilities, Fe2+ chelating and anticholinergic activities. Cynarin demonstrated 87.72% inhibition of linoleic acid lipid peroxidation at 30 g/mL concentration. Conversely, some standard antioxidants like trolox, tocopherol, butylated hydroxytoluene (BHT), and butylated hydroxyanisole (BHA) exhibited inhibitions of 90.32, 75.26, 97.61, 87.30%, and opponent peroxidation of linoleic acid emulsion at the identical concentration, seriatim. Also, cynarin exhibited effective DMPD•+, ABTS•+, DPPH•, and H2O2 scavenging effects, reducing capabilities and Fe2+ chelating effects. On the contrary, IC50 and Ki parameters of cynarin for acetylcholinesterase enzyme inhibition were determined as 243.67 nM (r2: 0.9444) and 39.34 ± 13.88 nM, respectively. This study clearly showed that cynarin had marked antioxidant, anticholinergic, reducing ability, radical-scavenging, and metal-binding activities.