Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Demir, Selim(12)
- Turan, Ibrahim(11)
- Aliyazicioglu, Yuksel(8)
- Topal, Meryem(7)
- Şengül, Mustafa(6)
- Baltaci, Cemalettin(5)
- Topal, Fevzi(5)
- Öz, Mehmet(5)
- Ürkek, Bayram(5)
- Akar, Zeynep(4)
- Alwasel, Saleh H.(4)
- Durmaz, Lokman(4)
- Gulcin, Ilhami(4)
- Gülçin, Ilhami(4)
- Akmeşe, Osman(3)
- Bingol, Zeynebe(3)
- Gören, Ahmet C.(3)
- Karatas, Seyda Merve(3)
- Misir, Sema(3)
- Turan, İbrahim(3)
- Yapıcı, İsmail(3)
- Yildiz, Hilal(3)
- Aliyazicioglu, Yüksel(2)
- Altay, Kadriye(2)
- Baltacı, Cemalettin(2)
- Deger, Orhan(2)
- Fidan, Muhammed Said(2)
- Gocer, Hulya(2)
- Gül, Abdulkadir(2)
- Gültekin, Ergün(2)
- Gülçin, İlhami(2)
- Kalin, Pinar(2)
- Kiziltas, Hatice(2)
- Kose, Leyla Polat(2)
- Küçük, Murat(2)
- Okan, Onur Tolga(2)
- Sarikaya, S. Beyza Öztürk(2)
- Ucuncu, Osman(2)
- Şengül, Memnune(2)
- AKMEŞE, Osman(1)
Yayınlar
- MakaleAçık Erişim2021Investigation the Different Extraction Temperature Effect of Artvin Savsat Region Propolis PBS Extracts on Antioxidant Properties and Eryhrocyte Hemolysis Inhibition
Canbolat, Deniz; Turan, Ibrahim; Küpeli, Yunus Emre; Kilinç, Sedanur; Piliç, Sevim
Propolis, which has been used in traditional medicine for hundreds of years all over the world, is a resinous natural product with a complex content obtained by honey bees by mixing the buds of plants and pollen grains with enzymes they secrete. Propolis may have different contents due to the gathering region, plant source, bee variety and climate of the place where it is collected. Although there are many studies related to different regions, so far the propolis of the Savsat region in Artvin have not been studied yet. The aim of this study is to determine the antioxidant properties (determination of iron reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic and total flavonoid content) of propolis extracts of Artvin Savsat region prepared with phosphate buffer salt solution (PBS) prepared at different temperatures (45 and 60 degrees C) and to determine the erythrocyte hemolysis inhibition properties of the extracts. Total phenolic (11.91 +/- 0.5-24.14 +/- 1.38 mg gallic acid equivalent to g propolis) and total flavonoid (1.79 +/- 0.03-2.13 +/- 0.07 mg quercetin equivalent to g propolis) content of PBS extracts, iron power determination (12.80 +/- 0.36-27.15 +/- 0.12 mg ascorbic acid equivalent to g propolis), 2,2-diphenyl-1-picrilhydrazyl (DPPH) radical scavenging activity (%22.86 +/- 0.5-31.24 +/- 1.33) and erythrocyte hemolysis inhibition (%55.58 +/- 0.57-96.37 +/- 0.02) were determined using spectrophotometric methods. As a result of the experiments, it was determined that the extract with PBS obtained at a temperature of 60 degrees C was higher than the extract obtained at 45 degrees C, in terms of antioxidant properties and erythrocyte hemolysis inhibition ratio. Further studies are required to identify molecules that inhibit erythrocyte hemolysis in extracts and have antioxidant properties.
AtıfWoS4OpenAlex9TR Dizin5İncele - MakaleAçık Erişim2021The Effect of Rosa pimpinellifolia Extract on the Proliferation of Human Tumor Cells
Demir, Elif Ayazoglu; Demir, Selim; Türkmen, Nihal; Turan, Ibrahim
The aim of this study was to evaluate the antioxidant properties of R. pimpinellifolia and its cytotoxic effect against human colon (WiDr), liver (HepG2) and lung (A549) cancer cells compared with a normal fibroblast (BJ) cell line. The antioxidant properties of extract was determined using spectrophotometric methods, while the cytotoxic effect of extract was evaluated using MTT assay. The total phenolic content, total flavonoid content and reducing power values of extract were calculated as 16.4 +/- 0.4 mg gallic acid equivalent, 5.2 +/- 0.2 mg quercetin equivalent and 34.3 +/- 2.4 mg trolox equivalent per g sample, respectively. Although the extract exhibits selective cytotoxic effect on three cancer cell lines in a dose-dependent manner compared to BJ cells, the highest selectivity index value (2.7-fold) was obtained for the WiDr cell line. In conclusion, this is the first study to demonstrate the cytotoxic effect of R. pimpinellifolia on cancer cells. To demonstrate the molecular mechanism of this cytotoxic effect and to carry out in vivo studies may be a guide for future clinical studies.
AtıfWoS3OpenAlex5TR Dizin1İncele - MakaleAçık Erişim2021Evaluation of Cytotoxic Effect of Onosma armeniacum Extract on Various Cancer Cells
Demir, Selim; Ayazoglu Demir, Elif; Turan, Ibrahim; Özgen, Ufuk
The aim of this study was to investigate the antioxidant properties of the root extract of Onosma armeniacum and its cytotoxic effect against human liver (HepG2), lung (A549) and colon (WiDr) cancer cell lines compared with a normal fibroblast cell line. Antioxidant properties of the extract was determined using the colorimetric methods. The cytotoxic effect of the extract were investigated using MTT assay. The total phenolic content and reducing power values of extract were 26.1 +/- 0.4 mg gallic acid equivalent and 52.6 +/- 1.1 mg trolox equivalent per g sample, respectively. The extract exhibited selective cytotoxic effect in all studied cancer cell lines, especially HepG2 (3.6-fold). The results of this study reveal that O. armeniacum, which has been found to have acceptable antioxidant and cytotoxic effects, should be examined with more comprehensive studies.
AtıfWoS8OpenAlex12TR Dizin1İncele - MakaleYalnız künye2021Scopus SJRQ2In vitro Antioxidant and Cytotoxic Activities of Extracts of Endemic Tanacetum erzincanense Together with Phenolic Content by LC-ESI-QTOF-MS
Yapıcı, İsmail; Altay, Ahmet; Öztürk Sarıkaya, Beyza; Korkmaz, Mustafa; Atila, Alptuğ; Gülçin, İlhami; Köksal, Ekrem
In this study, phenolic composition, and in vitro biological activities of ethyl acetate (EAE) and methanol (ME) extracts obtained from the aerial parts of endemic Tanacetum erzincanense were investigated. Total phenolic and flavonoid content of the extracts were determined by Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. Antioxidant capacity of the extracts was evaluated over radical scavenging (DPPH and ABTS) and metal ion reducing power (FRAP and CUPRAC) tests. Individual phenolic compounds in ME was analyzed by high-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS). Cell inhibitory potential of the extracts was tested against colorectal adenocarcinoma (HT-29), breast adenocarcinoma (MCF-7), and hepatocarcinoma (HepG2) cells by 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. The results showed that ME contains higher TPC (64.4 mg GAE/g) and TFC (62.2 mg QE/g) than those of EAE (41.5 mg GAE/g and 40.0 mg QE/g). LC-ESI-QTOF/MS analysis revealed that ME is rich in phenolic compounds, namely, chlorogenic acid, apigenin, quercetin, luteolin, and diosmetin. Antioxidant assay results indicated that ME possess stronger activity than EAE and a power that competes with synthetic antioxidants. XTT assay results demonstrated that although both extracts displayed a considerable cytotoxicity against the tested cancer cell lines in a time and dose-dependent manner, ME expressed its selective inhibitory action towards MCF-7 cells with an IC50 value of 20.4 μg/mL for 72 h. These results may serve as a basis for further in vivo studies to examine the potential applications of T. erzincanense in food and pharmaceutical industries.
AtıfScopus27WoS24OpenAlex24İncele - MakaleYalnız künye2021Scopus SJRQ3Determination of the Chemical Composition, Antioxidant and Antimicrobial Activities of Different Parts of Rosa canina L. and Rosa pimpinellifolia L. Essential Oils
Öz, Mehmet; Deniz, Ilhan; Okan, Onur Tolga; Baltaci, Cemalettin; Karatas, Seyda Merve
Today, the use of essential oils is increasing day by day, and it is considered as raw materials in many industries. Therefore, this study aims to identify the essential oil profile, antioxidant and antimicrobial activity of Rosa canina L. and Rosa pimpinellifolia L. reveal their potential use in the industry. Essential oils that were obtained in 2013-2014 from different parts (flower, leaf, stem, and fruit) of Rosa canina L. and Rosa pimpinellifolia L. by the hydro-distillation method were analyzed by GC/MS. The dominant components in Rosa canina L. were found as trans-geraniol in the flowers (42.08 % for 2013), linalool in the leaves (11.55 % for 2014), and stem (14.91 % for 2013) and vitispirane in the fruits (37.19 % for 2014). The dominant components in R. pimpinellifolia L. were found as heneicosane in the flowers (22.07 % for 2014), γ-elemene in the leaves (30.21 % for 2013), linalool in the stem (14.76 % for 2013) and the fruits, (E)-2-hexenal for those collected in 2013 (14.09 %). The IC50 and FRAP values of the samples ranged from 127.54 to 142.22 μg/g and 121.43 to 296.78 μmol TE/g, respectively. According to the results obtained, both R. canina L. and R. pimpinellifolia L. were found to show poor antioxidant values of DPPH and FRAP. In general, it was determined that the essential oils obtained from different plant parts of both species had a moderate antimicrobial effect against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, and Pseudomonas aeruginosa.
AtıfScopus17WoS16İncele - MakaleYalnız künye2021Scopus SJRQ4Chemical composition and biological activities of volatile oils of arabis alpina l. Ssp. alpina
Ucuncu, O.
This work reports volatile constituents, antimicrobial and antioxidant activity of volatile oils from the air-dried flower and aerial parts (APs) of Arabis alpina L. ssp. alpina. The volatile components of flower and aerial parts (stem + leaf) were investigated by means of hydrodistillation in Clevenger type apparatus and GC/MS/FID analysis. Fifty-one and fifty-three compounds in the volatile oils of flower and APs of A. alpina were identified. Terpene derivatives were the major volatiles in both oils. Hexahydrofarnesyl acetone was the main component of flower oil and APs oil in ratios of 16.27% and 26.94%, respectively. Additionally, both flower and APs essential oils were investigated for their antimicrobial activity against twelve bacteria and five fungi, using agar dilution method and antioxidant activities by using DPPH˙, ABTS˙+ and Folin-Ciocalteu assays. Flower oil was effective against B. subtilis and B. cereus even at 100µg/mL. The amount of total phenolic, %DPPH˙ scavenging activity and ABTS˙+ scavenging activity were found as 485.60±7.28 mg/mL GAE, 49.85±1.22% and 166.43±12.05µM Trolox equivalent, respectively. Flower oil exhibited antimicrobial effect against gram positive bacteria especially, and moderate antioxidant activity.
AtıfScopus3WoS2İncele - MakaleAçık Erişim2021Scopus SJRQ3Determination of Antimicrobial and Antioxidant Activities and Chemical Components of Volatile Oils of Atropa belladonna L. Growing in Turkey
Öz, Mehmet; Fidan, Muhammed Said; Baltaci, Cemalettin; Ücüncü, Osman; Karatas, Seyda Merve
In this study; the essential oils were obtained from the flowers and leaves of Atropa belladonna L. plants through the hydrodistillation method in a Clevenger type device. The chemical composition of volatile oils was determined by analyzing with the GC-MS/MS instrument. The antioxidant activity of the obtained volatile oils was determined according to total antioxidant content (TAC), free radical scavenging (ABTS) capacity, free radical scavenging (DPPH) amounts, total flavonoid content (TFC), total phenolic content (TPC), and ferric reducing antioxidant power (FRAP) capacity methods. Antimicrobial activities of essential oils were determined through the agar-disc diffusion method. As for the results; volatile oil yields of the A. belladonna were determined as 0.37 % in leaves and 1.57 % in flowers. The highest percentage of chemical compounds in essential oils of the A. belladonna plant were determined as (43.75 %) aldehydes in leaves and as (39.96 %) fatty acids in flowers. In addition, the main constituents found in volatile oils were eicosane (35.92 %) in the leaves and hexadecenoic acid (18.84 %) in the flowers of the A. belladonna. The highest amounts of antioxidant for the test specimens were found as 1.37-1.48 mg QE/100 mL in TFC, as 13.25-21.91 mg AA/100 mL in ABTS, as 51.49-62.87 mg GA/100 mL in TPC, and as 4.71-7.97 mg FeSO4/100 mL in FRAP capacity. As the results of the antimicrobial activities, it was determined that only the leaves of A. belladonna show antimicrobial activity against the mold-yeast and gram-positive and gram-negative bacteria microorganisms among the 17 bacteria and 6 yeast-mold types used in the study.
AtıfScopus12WoS8OpenAlex11İncele - MakaleYalnız künye2021Scopus SJRQ2Effects of the addition of rose hip on various nutritional and quality properties of ice cream
Ürkek, Bayram
In this study, the effects of rose hip (Rosa L.) on the physical, chemical and sensory characteristics, antioxidant activity and mineral contents of ice cream were investigated. Four different proportions (0, 5%, 10% and 15%) of rose hip were used in ice cream production. The sample containing 15% rose hip had the highest antioxidant activity (DPPH and total phenolic content, 28.71 mg sample mg-1 DPPH and 1555 mg GAE g-1 extract, respectively) and vitamin C (246.50 mg 100 g-1) among all ice cream samples. The L* and White Index values of the samples decreased significantly with the increment of rose hip concentration while the a* and b* values increased. The calcium, magnesium, sulfur, potassium, manganese, iron and zinc values increased with the addition of rose hip. Sensory results showed that the ice cream sample containing 15% rose hip had lower score than the control sample in terms of color, appearance, gumming structure, flavor and general acceptability.
AtıfScopus6WoS3İncele - MakaleAçık Erişim2021Scopus SJRQ1LC-HRMS Profiling and Antidiabetic, Anticholinergic, and Antioxidant Activities of Aerial Parts of Kinkor (Ferulago stellata)
Kiziltas, Hatice; Bingol, Zeynebe; Gören, Ahmet Ceyhan; Kose, Leyla Polat; Durmaz, Lokman; Topal, Fevzi; Alwasel, Saleh H.; Gulcin, Ilhami
Kinkor (Ferulago stellata) is Turkish medicinal plant species and used in folk medicine against some diseases. As far as we know, the data are not available on the biological activities and chemical composition of this medicinal plant. In this study, the phytochemical composition; some metabolic enzyme inhibition; and antidiabetic, anticholinergic, and antioxidant activities of this plant were assessed. In order to evaluate the antioxidant activity of evaporated ethanolic extract (EEFS) and lyophilized water extract (WEFS) of kinkor (Ferulago stellata), some putative antioxidant methods such as DPPHGreek ano teleia scavenging activity, ABTS(center dot+) scavenging activity, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+)-binding activities were separately performed. Furthermore, ascorbic acid, BHT, and alpha-tocopherol were used as the standard compounds. Additionally, the main phenolic compounds that are responsible for antioxidant abilities of ethanol and water extracts of kinkor (Ferulago stellata) were determined by liquid chromatography-high-resolution mass spectrometry (LC-HRMS). Ethanol and water extracts of kinkor (Ferulago stellata) demonstrated effective antioxidant abilities when compared to standards. Moreover, ethanol extract of kinkor (Ferulago stellata) demonstrated IC50 values of 1.772 mu g/mL against acetylcholinesterase (AChE), 33.56 +/- 2.96 mu g/mL against alpha-glycosidase, and 0.639 mu g/mL against alpha-amylase enzyme respectively.
AtıfScopus66WoS62OpenAlex64İncele - MakaleAçık Erişim2021Scopus SJRQ1Antidiabetic, anticholinergic and antioxidant activities of aerial parts of shaggy bindweed (Convulvulus betonicifolia Miller subsp.) - profiling of phenolic compounds by LC-HRMS
Bingol, Zeynebe; Kiziltas, Hatice; Gören, Ahmet C.; Kose, Leyla Polat; Topal, Meryem; Durmaz, Lokman; Alwasel, Saleh H.; Gulcin, Ilhami
In order to evaluate the antioxidant activity of evaporated ethanolic extract (EESB) and lyophilized water extract (WESB) of Shaggy bindweed (Convulvulus betonicifolia Mill. Subs), some putative antioxidant methods such as DPPH. scavenging activity, ABTS(center dot)(-) scavenging effect, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+) binding activities were separately performed. Also, ascorbic acid, alpha-tocopherol and BHT were used as the standard compounds. Additionally, some phenolic compounds that responsible for antioxidant abilities of EESB and WESB were screened by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the same concentration, EESB and WESB demonstrated effective antioxidant abilities when compared to standards. In addition, EESB demonstrated IC50 values of 1.946 mu g/mL against acetylcholinesterase (AChE), 0.815 mu g/mL against alpha-glycosidase and 0.675 mu g/mL against alpha-amylase enzymes.
AtıfScopus84WoS83OpenAlex88İncele - MakaleAçık Erişim2020Scopus SJRQ2Biological activities and phytochemical screening of ethanol extracts from adonis paryadrica (Ranunculaceae)
Ucuncu, Osman; Baltaci, Cemalettin; Akar, Zeynep; Duzgun, Azer Ozad; Cuce, Mustafa; Kandemir, Ali
In this study, secondary metabolite profiles of flower, leaf and root of Adonis paryadrica ethanolic extracts were performed by using a newly developed and validated LC-MS/MS method. For this purpose, the LC-MS/MS system was used for the quantitative and qualitative analysis of 37 phytochemicals. The main component of the flower, leaf and root extracts was the quinic acid (160, 111 and 64 mg/kg, respectively). Antioxidant activities were determined using methods such as DPPH (SC50, mg/mL), FRAP (µM TEAC), CUPRAC (µM TEAC), ABTS (SC50, mg/mL) and TPC (GAE, µg/mL). While the highest antioxidant activity value for FRAP, CUPRAC, ABTS and TPC methods was determined in the leaf part of the plant, this value was determined in the flower part for DPPH. The antibacterial effects of the plant extracts were determined by liquid microdilution and agar diffusion methods. As a result, antimicrobial effect of flowers, leaf and all sections of plant extracts were determined except plant root extracts which did not display any activity against bacteria. Antimicrobial activity of the plant flower extract was found to be higher especially against to P. vulgaris with 3.5 mg/mL MIC.
AtıfScopus11WoS8OpenAlex17İncele - MakaleYalnız künye2020Scopus SJRQ2Degradation kinetics of bioactive compounds and antioxidant activity in strawberry juice concentrate stored at high and low temperatures
Menevseoglu, Ahmed; Dıblan, Sevgin; Türkyılmaz, Meltem; Özkan, Mehmet
In this study, changes in ascorbic acid (AA), phenolics, anthocyanins (ACNs) and antioxidant activity (AOA) were monitored during storage at − 25, 4, 10 and 20 °C to observe the stability of bioactive compound in strawberry juice concentrates (SJCs). Half-life periods (t1/2) for ACN and AA degradations were 7.9 and 50 days at 20 °C, respectively. On the contrary, much higher t1/2 values were found for the reduction in AOA and phenolics, i.e. 251 and 430 days at 20 °C, respectively. AOA and phenolics were more stable during storage than AA and ACNs. p-Coumaric acid was the major phenolic identified in SJC. p-coumaric acid (33.8%), catechin (23.1%), vanilic acid (18.6%) and epicatechin (16.1%), which constituted 91.6% of phenolics in SJC, followed by chlorogenic acid (4.9%) and caffeic acid (3.2%). Chlorogenic acid was the least stable phenolic in SJC stored at 20 °C. Strong correlations were found between AOA and ACNs (r = 0.99), phenolics (r = 0.94) and AA (r = 0.92) in SJC stored at 20 °C. Significant ACN and AA degradations occurred at storage temperature as low as 4 °C. Therefore, storage at − 25 ℃ was suggested for SJC to maximize the stabilities of especially ACNs and AA in SJC.
AtıfScopus19WoS17OpenAlex17İncele - MakaleAçık Erişim2019Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells
Demir, Selim; Turan, Ibrahim; Misir, Sema; Aliyazicioglu, Yuksel
Although some studies have evaluated the cytotoxic activity of different Fabaceae species, there has been only limited research into the cytotoxic effect of Dorycnium pentaphyllum. The purpose of this study was to determine the antioxidant properties and cytotoxic effect of D. pentaphyllum extract on various human cancer cells. The total phenolic content (TPC) of the extract was determined using colorimetric method. The cytotoxic effect of the extract on human lung (A549), liver (HepG2), and breast (MCF-7) cancer cells and a normal human fibroblast cells was assessed using the MTT assay. TPC value of extract was found as 140.3 +/- 1.1 mg gallic acid equivalent per g sample. Extract showed selective cytotoxicity on all studied cancer cells compared to normal fibroblast cells, and the IC50 values of the extract in the cancer cells range from 100.4 to 298.5 mu g/mL. This is the first study to reveal the cytotoxic effect of D. pentaphyllum extract on cancer cell lines. Phytomedical applications of D. pentaphyllum may represent promising approaches in the treatment of cancer.
AtıfWoS7OpenAlex7TR Dizin5İncele - MakaleYalnız künye2019Scopus SJRQ4DETERMINATION OF ANTIOXIDANT, ANTIMICROBIAL ACTIVITIES, PHENOLIC COMPOUNDS OF WILD ROSA L. SPECIES BAYBURT, TURKEY
Gidik, Betul; Akar, Zeynep; Can, Zehra; Sefali, Abdurrahman; Erturk, Omer
Five different rosehip (Rosa L.) species including, Rosa canina, Rosa iberica Stev, Rosa villosa, Rosa beggeriana Schrenk and same kind of but different location Rosa spinosissima grown in Bayburt from Turkey are studied. The wild species of Rosa L. were collected from Rocks and Steps, Field and garden borders. Morphological parameters were measured as fruit and seed weights and fruit and seed size. Ten fruits were chosen randomly for the measurement and take the average of them to determine the weights and size. In our study was determined antioxidant activity, antimicrobial activity and phenolic compounds. As antioxidant activity; total phenolic total flavonoid, condense tannin contents, CUPRAC, FRAP, ABTS. Nineteen standard of phenolic compositions of fruits and seeds were determined. Antioxidant, antimicrobial activities of the extracts were found high levels in rose hip species fruit than seed. Catechin, rutin protocatequic acid and CAPE were principal for all rose hip species fruit but, was not deteceted CAPE Kop Mountain, Yukari Kop Village Rosa spinosissima species. This suggests that the difference in location affects the phenolic content. Pearson Correlation analyze has done with the morphological parameters, GPS data, antioxidant activity and phenolic compounds by using the SPSS software programme version 25.0.
AtıfWoS3İncele - MakaleAçık Erişim2019Antioxidant Properties of Primula vulgaris Flower Extract and Its Cytotoxic Effect on Human Cancer Cell Lines
Demir, Selim; Turan, Ibrahim; Aliyazicioglu, Yuksel
Primula vulgaris is a plant belonging to Primula genus, and these species are often used in traditional therapy. Although there are numerous studies on the antioxidant and cytotoxic effects of different Primula species, there are limited studies of these properties of P. vulgaris extracts. The aim of this study was to determine the antioxidant properties and cytotoxic effects of the extract prepared with dimethyl sulfoxide from P. vulgaris flowers. The total phenolic content, total flavonoid content, and reducing antioxidant power of the extract were determined using spectrophotometric methods. The cytotoxic effect of the extract on human colon (WiDr), lung (A549), liver (HepG2), breast (MCF-7), and prostate (PC-3) cancer cells and a normal human fibroblast cell line was assessed using the MTT assay. Total phenolic content and reducing power values of extract were found 33.02 +/- 0.92 mg gallic acid equivalents, and 64.86 +/- 1.18 mg trolox equivalents per g sample, respectively. Extract exhibited selective cytotoxic effect on all studied cancer cells compared to normal fibroblast cells, and the IC50 values of the extract in the cancer cell lines range from 191.8 to 375.3 mu g/mL. This work is one of the pioneering work that reveals the powerful antioxidant properties of P. vulgaris flower extract and its selective cytotoxic effect on cancer cell lines. Further studies are needed to identify the active molecules in the extract and the cytotoxic action mechanisms of these molecules.
AtıfWoS20OpenAlex22TR Dizin9İncele - MakaleAçık Erişim2019Scopus SJRQ3Secondary metabolites of ethanol extracts of pinus sylvestris cones from eastern anatolia and their antioxidant, cholinesterase and α-glucosidase activities
Topal, Meryem
Scots pine (Pinus sylvestris) is the most widely distributed tree species among pine species. Antiradical, antioxidant properties and inhibition properties on butyrylcholinesterase (BChE), acetylcholinesterase (AChE) and α-glycosidase activities of ethanol extracts of P. sylvestris were reported from Sarikamis (Kars), Gumushane and Erzurum provinces of Turkey. The cones of P. Sylvestris from Gumushane showed the highest IC50 values in both DPPH. (14.75 µg/mL) and ABTS.+ (12.56 µg/mL) radical scavenging activities. An LC-HRMS method developed and the secondary metabolite composition of extracts were identified. The major compounds are determined as (+)-trans taxifolin, quercitrin, fumaric acid, (-) epicatechin, and nepetin-7-O-glucoside, apigenin-7-O-glucoside in P. sylvestris cones.
AtıfScopus65WoS60OpenAlex47TR Dizin21İncele - MakaleYalnız künye2019Scopus SJRQ2Antioxidant Activity, Physiochemical and Sensory Characteristics of Ice Cream Incorporated with Sloe Berry (Prunus spinosa L.)
Ürkek, Bayram; Şengül, Mustafa; Akgül, Halil İbrahim; Kotan, Tuba Erkaya
The aim of this study was to determine the influence of sloe berry (SB) addition at different ratios on the physical, chemical, antioxidant, colour and sensory properties and mineral contents of ice creams. The increment of SB led to the an increase of titratable acidity, overrun, viscosity and ash values, first dripping and complete melting times; in contrast, it decreased the total solid, pH and fat values. Mn and Zn values were affected significantly (P < 0.05) by the addition of SB but other element levels were not affected. L* and b* values decreased significantly in all samples as compared with control sample, while a*, H°, C* and ∆E* increased significantly with addition of SB. Antioxidant activity was the highest in the sample containing 15 % SB (w/w) among ice cream samples. The ice cream samples containing 15 % SB had higher score than the control sample in terms of colour and appearance, gumming structure and general acceptability.
AtıfScopus26OpenAlex26İncele - MakaleAçık Erişim2019Scopus SJRQ2Anticholinergic, antidiabetic and antioxidant activities of cinnamon (cinnamomum verum) bark extracts: polyphenol contents analysis by LC-MS/MS
Gulcin, Ilhami; Kaya, Ruya; Goren, Ahmet C.; Akincioglu, Hulya; Topal, Meryem; Bingol, Zeynebe; Cakmak, Kader Cetin; Sarikaya, Sevim B. Ozturk; Durmaz, Lokman; Alwasel, Saleh
Ethanolic (EEC) and aqueous (WEC) extracts of cinnamon (Cinnamomum verum) were evaluated for their antioxidant profiles by eight distinguished bioanalytical antioxidant methods. Their inhibitory effects were tested against some enzymes including acetylcholinesterase, butyrylcholinesterase, alpha-glycosidase and alpha-amylase, which linked to different diseases. Additionally, the antioxidant properties were determined and polyphenolic compositions of the both extracts were evaluated by LC-MS/MS analysis. According to the LC-MS/MS experiments, thirteen compounds were found in WEC and EEC. Also, p-hydroxybenzoic acid (321.1 mg/kg extract), p-coumaric acid (291.4 mg/kg extract), and pyrogallol (142.4 mg/kg extract) were found to be the most abundant ingredients in the WEC. On the other hand, pyrogallol (264.3 mg/kg extract), ferulic acid (224.7 mg/kg extract) and p-coumaric acid (170.2 mg/kg extract) were found as the most plentiful chemicals in the EEC. For the estimation of the antioxidant capacities of the both extracts (WEC and EEC), DPPH center dot and ABTS(center dot+) scavenging activities, as well as Fe3+-Fe2+, and Cu2+-Cu+ reducing assays were studied. The IC50 values of the WEC and EEC indicated that they were potent effective DPPH center dot (21. 25 and 15.71 mu g/mL) and ABTS(center dot+) (6.52 and 5.79 mu g/mL) scavengers, as well as AChE (221.33 and 110.26 mu g/mL), BChE (461.69 and 94.93 mu g/mL), alpha-glycosidase (206.86 and 220.00 mu g/mL) and alpha-amylase (189.86 and 200.86 mu g/mL) inhibitors. As a conclusion, both EEC and WEC had rich phenolic contents and demonstrated effective anticholinergic, antidiabetic and antioxidant effects.
AtıfScopus202WoS172OpenAlex178İncele - MakaleAçık Erişim2018Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines
Demir, Selim; Turan, Ibrahim; Aliyazicioglu, Yuksel
Rhododendron luteum belongs to the genus Rhododendron, members of which are frequently used in folk medicine. Various studies have investigated the cytotoxic effect of different Rhododendron species, but there have been limited studies of the cytotoxic effect of R. luteum. The aim of this study was to investigate the antioxidant properties and cytotoxic effect of R. luteum leaf extract on the human cancer cells. The total phenolic content (TPC), total flavonoid content (TFC), and reducing power of the extract were evaluated using spectrophotometric procedures. The cytotoxic effect of the extract on five cancers (human breast, colon, lung, prostate, and liver carcinoma) and human fibroblast cells was determined using the MTT assay. TPC and reducing power values of extract were found 173.2 +/- 3.97 mg gallic acid equivalents, and 629.5 +/- 3.49 mg trolox equivalents per g sample, respectively. R. luteum leaf extract exhibited selective cytotoxicity especially against colon (1.9 fold) and liver (2.2 fold) cancer cells compared to normal fibroblast cells. This is the first study to reveal the cytotoxic effect of R. luteum leaf extract. Further studies are now needed to identify the cytotoxic molecules in the extract and their mechanisms.
AtıfWoS15İncele - MakaleAçık Erişim2018Scopus SJRQ3Antioxidant properties and protective effect of Turkish propolis on t-BHP-induced oxidative stress in foreskin fibroblast cells
Misir, Sema; Aliyazicioglu, Yuksel; Demir, Selim; Turan, Ibrahim; Yaman, Serap Ozer; Deger, Orhan
Objective: Propolis is an important bee product, rich in polyphenolic compounds. It has antitumoral, antioxidant, antimutagenic, and other useful activities. Biological activities of propolis are generally attributed to its substance of polyphenolic compounds. The aim of this study was to investigate the in vitro antioxidant properties and reduction amount of intracellular reactive oxygen species (ROS) in human normal foreskin fibroblast cells by Turkish propolis ethanolic extract (EEP). Method: Total phenolic and flavonoid contents, reducing power, radical scavenging capacity, and iron chelating activity of EEP were determined using spectrophotometric methods. Intracellular ROS levels were determined by spectrofluorometric analysis with CM-H2 DCFDA. Results: Our results indicated that antioxidant activity was correlated with the higher total phenolic and flavonoid contents of EEP. The generated ROS levels were significantly decreased by EEP compared to only t-BHP group. Conclusion: Ethanolic propolis extract may be evaluated as a potency antioxidant resource and/or a novel natural agent in food and drug industries.
AtıfScopus11WoS15OpenAlex16İncele