SDG 3 · Sağlık ve Kaliteli Yaşam · SDG · Gümüşhane Üniversitesi

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MakaleAçık Erişim2026/r/gumushane/12128
Chemical Profiling and Biological Activities of Methanol Extracts and Essential Oils From Ferulago Platycarpa Boiss. & Balansa (Endemic): Enzyme Inhibition, Antioxidant, and Antimicrobial Properties
Aygül, İmdat; Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman
This study aimed to evaluate the phytochemical composition and biological activities of Ferulago platycarpa methanol extract (ME) and essential oil (EO). The EO was obtained via hydrodistillation and analyzed by GC-MS, revealing sesquiterpene hydrocarbons as the dominant class, with caryophyllene (65.8%) and α-pinene (9.65%) as the major constituents. The methanol extract was subjected to LC-MS/MS analysis, which identified eleven phenolic compounds, with chlorogenic acid as the most abundant. The extract showed significantly higher total phenolic (1196.22±11.64 mg GAE/100g) and antioxidant (1870.00±17.69 mg QEE/100g) contents compared to the ME. In vitro enzyme inhibition assays demonstrated that the methanol extract exhibited potent inhibitory activity against carbonic anhydrase II (CA-II, ICSO= 0.023 pg/mL), acetylcholinesterase (AChE, ICSO= 110 pg/mL), and butyrylcholinesterase (BChE, IC50= 390 pg/mL). In contrast, the EO showed higher inhibition against α-amylase (IC50= 5920±10.45 pg/mL) and BChE (IC50= 1.32±0.65 pg/mL), while its α-glucosidase showed no inhibition. Antioxidant assays indicated superior activity for the methanol extract compared to the EO. Furthermore, antimicrobial testing revealed that the EO demonstrated broader and more effective antimicrobial action, exhibiting lower MIC and MBC values against several bacterial and fungal strains. Collectively, these results highlight F. platycarpa as a valuable source of bioactive compounds with promising applications in antidiabetic, neuroprotective, antioxidant, and antimicrobial therapies.
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Kitap BölümüYalnız künye2026/r/gumushane/12126
Smart Nanocarriers and AI-Driven Release Systems for Targeted Antibiotic Delivery
Kostas, Rabia Yasa; Bozbeyoglu, Pinar; Altuner, Elif Esra; Elmas, Muzaffer
The antimicrobial resistance (AMR) crisis is not merely a consequence of the emergence ofresistant pathogens. Furthermore, the remaining shortcomings oftraditional antibiotic production and development processes have become a profound health threat, exacerbated by their pharmaceutical and pharmacokinetic limitations. This book chapter redefines the AMR crisis as a problem of drug and treatment discovery as well as drug delivery to target tissues, and examines the technological angle emerging at the intersection of nanotechnology and artificial intelligence (AI) in response to this problem. This review begins by examining the problems inherent in traditional antibiotic architecture, such as systemic toxicity, low bioavailability, poor stability, and, most importantly, the inability to penetrate protected sites such as bacterial biofilms or intracellular bunkers. Copyright © 2026 by IGI Global Scientific Publishing. All rights reserved. No part of this publication may be reproduced, stored or distributed in any form or by any means, electronic or mechanical, including photocopying, without written permission from the publisher. Use of this publication to train generative artificial intelligence (AI) technologies is expressly prohibited. The publisher reserves all rights to license its use for generative AI training and machine learning model development.
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MakaleAçık Erişim2026/r/gumushane/12121
The Effect of Teamwork Attitudes on Job Performance: A Study Among Nurses; [Ekip Performansı Tutumunun İş Performansına Etkisi: Hemşireler Örnekleminde Bir Araştırma]
Köse, Aslı
Aim: To evaluate the impact of nurses’ teamwork attitudes on their job performance. Method: A descriptive, correlational quantitative research design was employed. The sample consisted of 350 nurses who worked at a city hospital in Turkey and voluntarily participated. Data were analyzed using SPSS 25 to assess differences in teamwork attitudes and job performance according to nurses’ educational levels and years of professional experience. Results: The analysis revealed no statistically significant differences in teamwork attitudes or job performance based on educational level. The mean total score for teamwork attitudes was (3.27±0.80), while the mean total score for job performance was 3.25 (SD=0.82). A strong positive correlation was found between teamwork attitudes and job performance (r = 0.764; p < 0.001). A strong positive correlation was found between teamwork attitudes and job performance, with the former accounting for 58.4% of the variance in the latter. Conclusion: This study found that nurses working in a city hospital exhibited moderate attitudes toward teamwork and moderate job performance, and that these two measures were strongly positively correlated.
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MakaleYayıncıda açık erişim2026/r/gumushane/12118
Effects of different conditioning activities performed after soccer-specific warm-up on post-activation performance enhancement
Kartal, Alper; Ilhan, Muhammet Taha; Ergin, Esin; Pancar, Zarife; Karaca, Burak; Yildiz, Yasin; Gulsen, Doğukan Batur Alp; Bayrakdaroğlu, Serdar
The post-activation performance enhancement (PAPE) effect has been widely studied; however, studies conducted under real-world conditions remain limited. Furthermore, investigations into how different conditioning activities (CAs) influence PAPE responses are also scarce. Therefore, this study investigates the acute effects of high-intensity barbell hip thrust (BHT) and back squat (BS) exercises, performed after a soccer-specific warm-up (Wup), on physical performance in soccer players. Seventeen male amateur soccer players participated in the study (mean age: 22.29 ± 3.60 years; height: 1.78 ± 0.06 m; body weight: 73.18 ± 8.44 kg). Participants attended 5 experimental sessions. In the first session, after anthropometric assessments, participants were familiarized with the exercises and tests. In the second session, one-repetition maximum (1RM) was tested for BS and HT exercises. In the following three sessions, two PAPE protocols and one traditional (TRAD) protocol were performed in a randomized order. In the TRAD protocol, players performed physical performance tests including countermovement jump (CMJ), 10-m and 30-m sprint, and zigzag agility 16 minutes after a soccer-specific Wup. In the PAPE protocols, players performed CAs (e.g., hip thrust or back squat) at 85% of 1RM for 2 sets of 3 repetitions following a soccer-specific Wup, and completed the tests 8 minutes later. Results from the linear mixed-effects models revealed no significant effect of protocol on any performance variable, including CMJ (F(2,32) = 1.554, p = 0.227), 10 m sprint (F(2,32) = 0.242, p = 0.786), 30 m sprint (F(2,32) = 0.453, p = 0.640), and change of direction (COD) performance (F(2,32) = 0.946, p = 0.399). Marginal R² values indicated that the protocol explained only a minimal proportion of variance in performance outcomes (0.005–0.030), whereas conditional R² and ICC values suggested that between-participant differences accounted for a substantial proportion of variance, particularly for CMJ and 30 m sprint performance. Bonferroni-adjusted pairwise comparisons further showed no significant differences between PAPEBHT, PAPEBS, and TRAD protocols for CMJ, 10 m sprint, 30 m sprint, or COD performance (all p > 0.05). In conclusion, neither PAPE protocol performed after a soccer-specific warm-up significantly enhanced or impaired subsequent CMJ, sprint, or COD performance compared with the traditional warm-up. These findings suggest that adding PAPEBHT or PAPEBS protocols after a soccer-specific warm-up may maintain subsequent performance without inducing measurable decrements; however, their ergogenic effectiveness may depend on individual factors such as players’ strength levels, recovery capacity, training status, and physical characteristics.
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Derleme MakaleYalnız künye2026/r/gumushane/12115
Effectiveness of ShotBlocker for Pain in Adult Needle-Related Procedures: Systematic Review and Meta-Analysis
Korkmaz, Emine; Uzun, Sevda; Aydın, Esra
Objectives This study was conducted to evaluate the effect of ShotBlocker use on pain levels during needle-related procedures in adults. Design Systematic review and meta-analysis. Data sources Studies were searched in the PubMed, Web of Science, ScienceDirect, Google Scholar, and Higher Education Council (YÖK) Thesis Center databases without any year restrictions. Review/Analysis methods The search and study selection were conducted in accordance with the PRISMA 2020 guidelines, and 18 studies meeting the inclusion criteria were included in the analysis. Data extraction and methodological quality assessment were performed using the Joanna Briggs Institute (JBI) critical appraisal tools. Findings were evaluated using meta-analysis and narrative synthesis methods. Results In this systematic review and meta-analysis, ShotBlocker was found to be effective in reducing pain levels in adults (SMD = −1,011; 95% CI: −1.390 to −0.631; Z = −5,221; p < .001; I² = 91.4%). However, substantial heterogeneity was observed among the included studies (I² = 91.4%). Conclusions This study demonstrates that ShotBlocker may be an effective nonpharmacological method for reducing pain levels during needle-related procedures in adults. However, the results should be interpreted with caution because of the substantial heterogeneity among the included studies.
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MakaleAçık Erişim2026/r/gumushane/12105
Investigation of the enzyme-inhibitory, antibacterial, and anticancer properties of metal phthalocyanines
Topal, Meryem; Topal, Fevzi; Yilmaz, Fırat; Bulut Atalay, Esra; Koçyiğit, Ümit Muhammet; Güzel, Emre
In this study, the enzyme inhibitory, antibacterial, and anticancer activities of a phthalonitrile derivative and its metal phthalocyanines (Pcs; indium, zinc, copper, cobalt, and manganese; 1–6) were investigated. The cobalt and manganese Pcs were synthesized for the first time in this study. The inhibitory activities of the symmetric Pcs, which were expected to interact with acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) via their 4-(isopropylbenzyl)oxy substituents through host–guest interactions, were investigated. More selective inhibition of AChE than BChE was observed for these compounds. For AChE, CoPc was identified as the most potent inhibitor (IC50 = 14.81 nM), whereas for BChE, InPc was identified as the most potent inhibitor (IC50 = 56.35 nM). The Ki values indicated that most compounds exhibited competitive inhibition; copper phthalocyanine (CuPc) showed particularly strong inhibition against AChE (Ki = 3.08 nM ± 1.12), whereas the lowest Ki value against BChE was observed for MnPc (Ki = 25.98 ± 1.97 nM). Most compounds exhibited competitive inhibition; however, CuPc showed competitive inhibition toward AChE but a noncompetitive inhibition pattern toward BChE. Dual inhibition of AChE and BChE by these compounds may be promising for addressing cholinergic deficits associated with Alzheimer’s disease. In addition, the acceptability of compounds 1–6 with respect to pharmacological drug-likeness criteria was assessed based on predicted absorption, distribution, metabolism, excretion (ADME) outcomes. In antibacterial tests, varying levels of inhibition were observed against selected bacterial strains. In anticancer assays, all compounds exhibited high cytotoxicity against the MCF-7 breast cancer cell line. Higher antiproliferative activity was observed for CuPc than for the other compounds. Morphological changes were induced in cancer cells by CuPc and MnPc. Overall, these compounds may have potential as enzyme inhibitors and as antibacterial and anticancer agents.
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MakaleAçık Erişim2026/r/gumushane/12099
Evaluation of antioxidant and antiproliferative activities of shogaol and its modulatory effects on key metabolic enzymes
Yapıcı, İsmail; Gülçin, İlhami
Phenolic compounds are versatile bioactive molecules, particularly in food applications. In this study, the antioxidant capacity of Shogaol, a member of the phenolic class, was evaluated using several methods. Additionally, its inhibitory effects on human carbonic anhydrase I and II (hCA I and II), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glycosidase, and α-amylase enzymes, as well as its antiproliferative effects on A-549 lung cancer, HT-29 colon cancer, MCF-7 and MDA-MB-453 breast cancer cell lines, and the 3T3-L1 mouse fibroblast normal cell line, were investigated. To determine the antioxidant capacity of Shogaol, DPPH and ABTS radical scavenging assays, as well as Cu²⁺, Fe³⁺, and FRAP reduction methods, were employed. Its inhibitory effects on hCA I and II, AChE, BChE, α-glycosidase, and α-amylase enzymes were examined. Furthermore, cell counting was performed using the TBE method, and cell viability was measured with the XTT assay to assess the antiproliferative effects of Shogaol on A-549, HT-29, MCF-7, MDA-MB-453, and 3T3-L1 cell lines. Shogaol demonstrated higher antioxidant capacity than BHA, BHT, and Trolox in the FRAP assay (60 µg/mL), higher than BHT in the DPPH scavenging assay, and higher than Trolox in the ABTS, Cu²⁺, and Fe³⁺ reduction assays. Shogaol also exhibited significant inhibitory effects against hCA I, hCA II, AChE, BChE, and α-glycosidase, with IC₅₀ values of 75.91, 47.04, 37.44, 24.11, and 35.72 nM, respectively. Moreover, Shogaol showed a more potent antiproliferative effect than the standard chemotherapeutic drug Carboplatin in A-549 lung cancer and HT-29 colon cancer cell lines, and greater efficacy than Docetaxel in the MCF-7 breast cancer cell line.
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MakaleYalnız künye2026/r/gumushane/10697
Efficacy and safety of doxorubicin-ifosfamide versus gemcitabine-docetaxel as first-line therapy in advanced nonuterine leiomyosarcoma: A multicenter real-world study
Tunbekici, Salih; Sahin, Gokhan; Yuksel, Haydar Cagatay; Acar, Caner; Akin, Imge; Aslanhan, Hasan; Isci, Mert; Kabaoglu, Ceren; Bal, Hamit; Sakin, Abdullah
Nonuterine leiomyosarcoma (NU-LMS) is a rare and biologically distinct subtype of soft tissue sarcoma (STS) with clinical behavior differing from that of uterine leiomyosarcoma. In advanced or metastatic STS, immunotherapy has demonstrated only modest activity, and chemotherapy remains the cornerstone of treatment. However, there is a persistent unmet need for more effective and better-tolerated options in this population. This multicenter, retrospective study included 150 patients with histologically confirmed metastatic NU-LMS who received first-line doxorubicin-ifosfamide (AI) or gemcitabine-docetaxel (GD) at 26 oncology centers in Turkey between 2010 and 2024. The primary endpoint was overall survival (OS). Survival was analyzed using Kaplan-Meier estimates and Cox proportional hazards models. Of 150 patients, 79 received AI and 71 received GD. Median OS for the entire cohort was 21.0 months. Median OS was 22.0 months with AI and 19.1 months with GD (p = .18), median progression-free survival was 7.3 versus 6.1 months (p = .66), and overall response rate was 25.3% versus 29.6% (p = .58), indicating no statistically significant differences in efficacy between the two regimens. Hematologic toxicities were less frequent with GD. AI and GD demonstrated comparable efficacy as first-line regimens for advanced or metastatic NU-LMS, with fewer hematologic toxicities observed for GD. Tumor location may have prognostic implications. These findings support individualized treatment selection and highlight the need for prospective studies in this rare and understudied sarcoma subtype, while the recent LMS-04 trial underscores the importance of future comparisons with doxorubicin-trabectedin to further refine first-line strategies in LMS.
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MakaleAçık Erişim2026/r/gumushane/10693
Preferences for mechanical ventilation modes among intensivists in Türkiye: a nationwide point-prevalence study
Yildirim, Suleyman; Kilinçözgün, Nurhayat; Alpdogan, Ozcan; Uçar, Huseyin; Taskiran, Imren; Ata, Adnan; Özcan, Ozhan; Öncü, Kivanc; Acarbay, Saba Mukaddes; Güner, Temel
Background/aim: Invasive mechanical ventilation (IMV) is a fundamental intervention for patients with respiratory failure in intensive care units (ICUs). This nationwide, multicenter point-prevalence study aimed to describe current mechanical ventilation mode preferences (conventional, adaptive, and biphasic) in Turkish ICUs and to report associated clinical outcomes descriptively, without assessing causal relationships. Materials and methods: A nationwide, multicenter point-prevalence study was conducted on 17 April 2024 and included adult patients (>= 18 years) who had been receiving IMV for more than 24 h. Data on patient demographics, ventilation mode distribution, ventilatory parameters, and descriptive clinical outcomes on day 28 (weaning status, tracheostomy, and mortality) were recorded without comparative outcome analysis. Results: A total of 426 patients were included. Conventional modes were used in 84.5% of patients, adaptive modes in 10.6%, and biphasic modes in 4.9%. Synchronized intermittent mandatory ventilation (SIMV) was the most commonly used conventional mode. The primary indication for IMV was acute respiratory failure (61%), with pneumonia being the leading cause. Among the 350 orotracheally intubated patients, 25.6% were in the weaning phase on the study day. A total of 59 (16.9%) patients were extubated, 150 (42.9%) underwent tracheostomy, and 64 (18.2%) remained intubated on day 28. Overall, 185 (43.4%) patients died during their ICU stay, 152 (35.7%) remained in the ICU, and 89 (20.9%) were successfully discharged from the ICU. Conclusion: Conventional ventilation modes, particularly SIMV, were more commonly used in Turkish intensive care units (ICUs), whereas adaptive modes were less frequently applied. These patterns may reflect factors such as clinician familiarity, institutional practices, and equipment availability rather than definitive preferences. Although the impact of ventilation modes on clinical outcomes was not comparatively evaluated in this study, the choice of ventilation mode may still influence patient outcomes. Therefore, further prospective and comparative studies are warranted to better elucidate this relationship.
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MakaleAçık Erişim2026/r/gumushane/10686
Ethical Principles and Frameworks in Dementia Care: A Scoping Review
Serrat, Rodrigo; Lauridsen, Sigurd; Bobrowicz-Campos, Elzbieta; Brandao, Tania; Brites, Rute; Cannella, Valentina; Celdrán, Montserrat; Chacur-Kiss, Karima; Cin, Aynur; Fabà, Josep
Providing ethical care for people living with dementia is essential for safeguarding their rights, dignity, and well-being. However, ethical decision-making in dementia care is complex, often involving tensions between competing moral claims such as autonomy, beneficence, nonmaleficence, justice, and non-discrimination. This scoping review aimed to map and describe the ethical principles, related normative considerations, and ethical frameworks discussed in the scientific literature on dementia care, and to identify how these have been conceptualized. Following the methodological guidance of Arksey and O'Malley (2005), Levac et al. (2010), and Peters et al. (2021, 2022), a comprehensive search across five databases (Web of Science, Scopus, CINAHL, Medline/PubMed, and PsycInfo) identified peer-reviewed papers published in English. In total, 388 papers published between 1981 and 2024 were included. The results revealed a wide range of ethical principles and related normative considerations frequently cited in the literature, although they were typically discussed in an isolated way without reference to other principles or broader ethical frameworks. In contrast, ethical frameworks were mentioned far less often, and when they were, they mostly reflected traditional approaches such as principlism. Four gaps were identified: the plurality of ethical frameworks and limited conceptual integration underpinning dementia care research and practice; insufficient attention to ethnic and socioeconomic diversity among people living with dementia; limited consideration of diverse care settings and geographical regions; and the limited inclusion of people living with dementia themselves in the studies reviewed. These findings underscore the need for conceptually integrative, inclusive, and context-sensitive approaches to dementia care ethics that can better inform both ethical reflection and care practices.
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MakaleAçık Erişim2026/r/gumushane/10661
Inspiratory muscle training enhances jumping power and shooting performance in elite air pistol athletes
Aydin, Ahmet Serhat; Yilmaz, Coskun; Söyler, Mehmet; Güney, Gamze; Eyüboglu, Ender; Karakulak, Izzet; Ceylan, Tulay; Altug, Tolga; Burac, Daniela Giconda; Sahinler, Yunus
Background Inspiratory muscle training (IMT) has been shown to enhance respiratory efficiency, postural stabilization, and neuromuscular coordination in athletic populations. However, its effects in precision sports such as air pistol shooting remain insufficiently explored. This study investigated the impact of a 4-week IMT program on respiratory muscle strength, explosive lower-limb performance, reaction time, and shooting accuracy in competitive air pistol athletes. Methods Twenty trained male air pistol athletes (age 18-35 years) were randomly assigned to an IMT group (n = 10) or a control group (n = 10). The IMT group performed supervised inspiratory muscle training twice daily (30 breaths/session), six days per week, for four weeks, using a threshold-loading device set initially at 40% of maximal inspiratory pressure (MIP), with progressive overload applied weekly. Both groups maintained their regular shooting training routines. Pre- and post-intervention assessments included countermovement jump (CMJ) and squat jump (SJ) performance (OptoJump), visual and auditory reaction time (Cognitech), and shooting performance evaluated via the SCATT system. A two-way repeated-measures ANOVA (group & times; time) was used for statistical analysis. Results Significant group & times; time interactions were observed for CMJ height (p < 0.001, eta & sup2;p = 0.618), CMJ power (p = 0.007, eta & sup2;p = 0.345), SJ height (p = 0.050, eta & sup2;p = 0.184), and SJ power (p = 0.050, eta & sup2;p = 0.181), indicating meaningful improvements in explosive performance in the IMT group. Shooting accuracy demonstrated a large and statistically significant interaction effect (p < 0.001, eta & sup2;p = 0.532), with substantial improvement observed only in the IMT group. Reaction time variables showed favourable but non-significant trends (p > 0.05). Conclusion A short-term IMT intervention significantly enhanced lower-limb explosive performance and shooting accuracy in competitive air pistol athletes without altering overall training load. These findings suggest that IMT may represent an effective, low-cost adjunct strategy to improve neuromuscular stability and fine-motor precision in precision-sport athletes.
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MakaleAçık Erişim2026/r/gumushane/10656
Comparison of plyometric repeated sprint and plyometric aerobic training on physical performance in youth soccer players
Selmi, Mohamed Amin; Hammami, Raouf; Ceylan, Halil Ibrahim; Duncan, Michael; Sassi, Radhouane Haj; Morgans, Ryland; Bayrakdaroglu, Serdar; Bragazzi, Nicola Luigi
High-intensity interval training (HIIT) and repeated-sprint sets (RSS) are established conditioning methods in soccer, yet their integration with plyometric training among highly trained youth soccer players remains underexplored. This study investigated the effects of an 8-week program combining plyometric training with either RSS (PLYO-RSS) or HIIT (PLYO-HIIT) on physical fitness in young soccer players. Twenty-nine male players (14-15 years) were randomly assigned to PLYO-RSS (n = 14) or PLYO-HIIT (n = 15). Both interventions lasted 20 minutes per session and replaced part of regular soccer training. Physical fitness assessments included countermovement jump (CMJ), 15-s repeated vertical jumps (15''RJ), five-jump test (5JT), 10 m and 30 m sprint, maximal aerobic speed (MAS), VO2max, and repeated sprint performance (total sprint time, best sprint, fatigue index). Physiological markers (lactate, rating of perceived exertion, and HRmax) were also monitored. Significant improvements over time were observed in muscle power, sprint speed, aerobic capacity, and repeated-sprint ability (p <= 0.001; eta & sup2;p = 0.339-0.489). However, no main effects or group & times; time interaction effects were observed, indicating no evidence of differential responses between the two training modes. In conclusion, integrating plyometric training with either RSS or HIIT effectively enhances physical fitness in highly trained youth soccer players, offering flexible options for training prescription.
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MakaleAçık Erişim2026/r/gumushane/10638
Chemical profiling, biological evaluation, and QSAR-informed metabolite prioritization of Hypericum ekerii: a first report on antioxidant, enzyme inhibitory, and cytotoxic activities
Zengin, Gokhan; Yagi, Sakina; Cetiz, Mehmet Veysi; Uba, Abdullahi Ibrahim; Nikolic, Filip; Gasic, Uros; Balos, Mehmet Maruf; Erharman, Ahmet; Dogan, Nurettin; Koyuncu, Ismail
Hypericum ekerii Y & uuml;ce and Ayta & ccedil;, first described in 2017, remains poorly characterized in terms of its phytochemical composition and biological properties. This study investigated aerial-part extracts of H. ekerii prepared with ethyl acetate, acetone, 70% acetone-water, and water. The hydroacetone extract showed the highest total phenolic and flavonoid contents. LC/MS analysis enabled the annotation of 99 constituents, including phenolic acids, flavonoids, xanthones, anthraquinones, and acylphloroglucinols. The hydroacetone extract exhibited the strongest radical-scavenging and reducing activities, whereas acetone and ethyl acetate extracts showed the highest metal-chelating capacity. In enzyme assays, hydroacetone was the most active extract against acetylcholinesterase, tyrosinase, and hCA II, while ethyl acetate showed greater inhibition of butyrylcholinesterase and hCA I. The aqueous extract was the most active against alpha-glucosidase, and hydroacetone and ethyl acetate showed comparable alpha-amylase inhibition. Cytotoxicity remained limited overall; the lowest IC50 value was observed for the ethyl acetate extract against HCT-116 cells (95.09 & micro;g mL-1), with weak selectivity (SI = 1.2). A QSAR-informed prioritization framework identified myricetin as the leading cross-layer candidate, p-coumaric acid as a target-specific priority metabolite, and ellagic acid, quercetin, and caffeic acid as antioxidant-priority candidates. Molecular docking supported favorable binding profiles for selected metabolites, while molecular dynamics simulations were used to assess the stability of representative complexes. Overall, H. ekerii appears to be a chemically diverse species with notable antioxidant and enzyme-inhibitory potential, providing a focused basis for future compound-level validation.
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MakaleAçık Erişim2026/r/gumushane/10625
Mono- and Bis-Ureido Sulfapyridines as Nanomolar Dual Cholinesterase Inhibitors: Enzyme Kinetics, Molecular Docking, and ADME/Tox Insights
Yapar, Gonul; Akocak, Suleyman; Lolak, Nebih; Topal, Meryem; Türkes, Cuneyt; Topal, Fevzi; Durgun, Mustafa; Koçyigit, Umit Muhammet; Isik, Mesut; Beydemir, Sukru
A series of mono- and bis-ureido sulfapyridine derivatives (SP-1-SP-10) were synthesized and evaluated as dual cholinesterase inhibitors targeting acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). All compounds exhibited nanomolar inhibition. The most potent AChE inhibitors were SP-8 (K-i = 5.25 +/- 0.73 nM), SP-9 (5.09 +/- 0.44 nM), and SP-10 (5.74 +/- 0.55 nM), significantly outperforming tacrine (K-i = 105.03 +/- 8.85 nM) under identical conditions. For BChE, SP-9 showed the strongest inhibition (K-i = 26.38 +/- 2.07 nM) compared with tacrine (172.03 +/- 32.42 nM). Kinetic analyses revealed competitive inhibition toward AChE for all derivatives, whereas BChE inhibition displayed both competitive and noncompetitive patterns depending on structural features. Glide XP docking using AChE (PDB: 7XN1) and BChE (PDB: 4BDS), complemented by MM-GBSA binding free energy calculations, supported favorable ligand accommodation within the catalytic pockets and highlighted the importance of aromatic and hydrophobic interactions. In silico ADME/Tox analysis revealed a potency-drug-likeness trade-off: mono-ureido derivatives complied with Lipinski's rule of five and showed high predicted oral absorption, whereas bis-ureido derivatives, despite superior enzymatic potency, exhibited multiple drug-likeness violations. These findings identify the ureido-sulfapyridine scaffold as a potent enzymatic dual cholinesterase inhibitory framework, warranting further structural optimization to improve pharmacokinetic properties.
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MakaleAçık Erişim2026/r/gumushane/10617
Short-Term Inspiratory Muscle Training Enhances Functional and Metabolic Health in Older Adults
Konca, Erkan; Yilmaz, Coskun; Bayrakdaroglu, Serdar; Ceylan, Halil Ibrahim; Arslan, Ayla; Ocak, Hakan; Karakulak, Izzet; Sari, Rifat; Uzun, Recep Nur; Soylu, Hakan Huseyin
Background: Age-related declines in respiratory muscle strength and ventilatory efficiency can impair functional capacity and metabolic health in older adults. Inspiratory muscle training (IMT) has been proposed as a practical intervention to counteract these changes, yet its systemic effects remain unclear. This study aimed to examine the effects of short-term IMT on functional capacity, diaphragm thickness, and liver tissue characteristics in healthy elderly men. Methods: Thirty community-dwelling men aged 60-80 years were randomly assigned to an IMT or control group. The IMT group performed four weeks of breathing exercises using a POWERbreathe (R) device at 40% of maximal inspiratory pressure, with a weekly 10% increase in pressure. Pre- and post-intervention assessments included the six-minute walk test (6MWT), diaphragm thickness and liver density via computed tomography, and quality of life (QoL; SF-12). Results: Four weeks of inspiratory muscle training significantly improved diaphragm thickness (11.7%), fatty liver density (FLD) (+16.7%), and six-minute walk performance (+5.3%), with large time x group effects favoring the IMT group. While the physical quality of life showed modest, comparable improvements, mental health outcomes demonstrated a moderate, time-dependent improvement without a significant group-by-time interaction. Conclusions: Short-term IMT improved diaphragmatic function and functional capacity in older men and was associated with favorable changes in a liver-related biomarker; however, given that only a single liver-related metric was assessed, these findings should not be interpreted as evidence of overall improvements in liver health.
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MakaleYayıncıda açık erişim2026/r/gumushane/10605
The mediating role of mental fatigue awareness in the relationship between perceived stress and psychological well-being among combat athletes
Yilmaz, Taner; Öz, Faik; Gül, Oguzhan; Dal, Suzan; Kara, Tayfun; Akkoç, Orkun; Küçük, Hamza; Fort, Patricia Roxana; Çitozi, Robert; Kadi, Mustafa Nurullah
Background Combat athletes face unique psychological demands, and stress may influence both their cognitive experiences and psychological well-being. This study aimed to examine the mediating role of mental fatigue awareness in the relationship between perceived stress and psychological well-being among combat athletes, thereby contributing to a clearer understanding of a potential cognitive mechanism linking stress to well-being in this population.Methods A total of 303 combat athletes voluntarily participated. Participants completed the Perceived Stress Scale, Mental Fatigue Awareness Scale, and Psychological Well-being Scale. Statistical analyses assessed the direct effects of perceived stress on psychological well-being, the effect of stress on mental fatigue awareness, and the potential mediating role of mental fatigue awareness.Results Perceived stress significantly increased mental fatigue awareness (b = 0.732, SE = 0.064, beta = 0.549, p = 0.001) and directly decreased psychological well-being (b = - 0.488, SE = 0.166, beta = - 0.191, p = 0.004). Mental fatigue awareness negatively affected psychological well-being (b = - 0.374, SE = 0.124, beta = - 0.195, p = 0.003) and partially mediated the relationship between stress and well-being (indirect effect: b = - 0.274, SE = 0.115, beta = - 0.107, p = 0.003; 95% CI = - 0.504 to - 0.049).Conclusion Perceived stress was associated with psychological well-being both directly and indirectly through increased mental fatigue awareness. These findings suggest that mental fatigue awareness may represent a relevant cognitive pathway linking stress perceptions to well-being. Accordingly, stress management and strategies aimed at regulating mental fatigue may be considered as potentially beneficial components in efforts to support psychological well-being among combat athletes.
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MakaleYalnız künye2026/r/gumushane/10603
Chemical characterization and bioactivity assessment of Erodium cedrorum subsp. salmoneum extracts supported by in silico analysis
Yagi, Sakina; Zengin, Gokhan; Zoltan, Cziaky; Jeko, Josef; Ivashchanka, Yahor; Turecka, Katarzyna; Halasa, Rafal; Gucwa, Magdalena; Kochan-Jamrozy, Kinga; Stefanowicz-Hajduk, Justyna
The growing interest in natural products, particularly as food additives and pharmaceutical raw materials, is making research into discovering new sources especially important. Erodium cedrorum subsp. salmoneum (family Geraniaceae) is used traditionally to cure many ailments like diabetes and gastro-intestinal diseases. Within this background, the current study was designed to investigate, for the first time, the chemical composition, antioxidant and enzyme inhibitory poroperties of different extracts from the aerial parts and roots of E. cedrorum. Results revealed that the aqueous extract of the aerial parts accumulated the highest total phenolic (85.35 mg GAE/g) and flavonoids (28.65 mg RE/g) contents. A total of 61 and 45 compounds, belonging mainly to phenolic acids and flavonoids, were tentatively identified in the aerial parts and roots extracts respectively. The aqueous extract from the aerial parts exerted the highest antiradical, ions reducing and chelation properties. The EtOH and 70% EtOH extracts of the two organs recorded the best anti-acetylcholinesterase activity (2.18 -2.41 mg GALAE/g; p >= 0.05) while those of the roots recorded respectively the best anti-butyrylcholinesterase and antityrosinase activities. The most potent antibacterial activity was exhibited by the 70% EtOH extracts from aerial parts and the pure EtOH extracts from roots. In contrast, ethyl acetate extracts from both plant parts showed only marginal activity. Among the tested strains, the highest susceptibility was observed against C. sporogenes ATCC 19404, C. bifermentans ATCC 638, S. aureus ATCC 6538, S. aureus ATCC 43300 (MRSA), C. diphtheriae PCM 506, and H. pylori ATCC 43504. The extracts were also examined for their anti-cancer potential on the HeLa, AGS and SKOV-3 cell lines, as well as a non-cancerous cell line. The ethyl acetate extract of the roots exhibited the most potent anti-cancer activity, with the lowest IC50 values. In addition, selected top-ranked complexes against AChE, alpha-amylase, representative bacterial and oncogenic targets were further evaluated by molecular docking and 100 ns molecular dynamics (MD) simulations, indicating overall conformational stability and persistent binding. In conclusion, these results position E. cedrorum as a valuable natural source of antioxidant and enzyme-inhibitory agents, meriting further exploration for drug development purposes.
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MakaleAçık Erişim2026/r/gumushane/10594
Effects of wrestling training on psychological well-being, anxiety, and resilience in adolescent boys
Özkan, Ridvan; Yilmaz, Coskun; Uzun, Recep Nur; Bayrakdaroglu, Serdar; Akar, Ozge Orhan; Öz, Faik; Çiçekli, Atif; Çiçekli, Aylin; Dal, Suzan; Ursu, Vasile Emil
Background Wrestling is a physically and mentally demanding combat sport that requires discipline, focus, and emotional control. Although its physiological effects are well documented, its psychological benefits in adolescents remain underexplored. This study aimed to examine whether a short-term, non-competitive wrestling program could enhance psychological well-being, reduce sport anxiety, and strengthen resilience in adolescent boys. Methods Thirty sedentary male adolescents (aged 12-15 years) were randomly assigned to a wrestling training group (n = 15) or a control group (n = 15). The intervention consisted of six weeks of supervised wrestling sessions performed three times per week. Psychological well-being (WHO-5), sport anxiety (SAS-2), and resilience (BPRS) were assessed before and after the intervention using the validated Turkish versions of the scales. Two-way repeated-measures ANOVA was used to analyze group & times; time interactions. Results Participants in the wrestling group demonstrated significant improvements in well-being (p < 0.001) and resilience (p < 0.001), alongside marked reductions in total sport anxiety and its subdimensions (p < 0.001), compared with the control group. All effects were large (eta p & sup2; = 0.52-0.94). Conclusion A structured six-week wrestling program produced meaningful psychological benefits in adolescent boys. Regular participation in non-competitive wrestling may serve as an effective approach to promote well-being, emotional stability, and adaptive coping skills during early adolescence, supporting the preventive and developmental role of combat sports in youth mental health.
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MakaleYalnız künye2026/r/gumushane/10587
Comparison of automated subcortical segmentation methods: VolBrain vs. 3D Slicer in brain volumetry
Acer, Niyazi; Palanci, Ozgur; Ipekten, Funda; Erdogan, Furkan Mert; Yarar, Burhan; Ozyasar, Ali Faruk
Objective:Accurate volume measurements of subcortical brain structures are essential in neuroimaging studies. This study aimed to compare two automated segmentation algorithms, 3D Slicer and VolBrain, for subcortical brain volumetry. Method:Subcortical volume measurements obtained using 3D Slicer and VolBrain were compared using the Neurofeedback Skull-stripped (NFBS) dataset. Statistical analyses included the coefficient of determination (R2) and Bland-Altman (B-A) plots. Volumetric measurements from both tools were evaluated across multiple subcortical brain regions. Results:Strong agreement was observed for the caudate nucleus, thalamus, and hippocampus, while notable discrepancies were identified in the amygdala, globus pallidus, and nucleus accumbens. Both constant and proportional systematic errors were detected in certain regions, with greater variability observed in 3D Slicer measurements. Intraclass Correlation Coefficient (ICC) analysis demonstrated weak agreement for the accumbens and amygdala, and moderate agreement for the hippocampus and thalamus, highlighting region-specific differences in performance Conclusion:Both VolBrain and 3D Slicer are effective tools for subcortical brain volumetry. VolBrain provides a fully automated workflow, whereas 3D Slicer allows greater flexibility through detailed corrections. Method selection should be guided by research objectives, regions of interest, and image quality. Further validation across diverse datasets is warranted to improve clinical applicability, particularly in regions showing lower agreement.
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MakaleAçık Erişim2026/r/gumushane/10552
In Vitro Antioxidant, Antibacterial, and Cholinesterase-Inhibitory Properties of an AqueousEuphorbia macrocladaRoot Extract Obtained by Ultrasound-Assisted Extraction
Gül, Abdulkadir; Yilmaz, Firat; Topal, Fevzi; Özgen, Inanc; Aydogmus, Ercan; Arslanoglu, Hasan
In the present study, the aqueous extract obtained from the roots of Euphorbia macroclada via ultrasound-assisted extraction (Ult-ER) was evaluated for its anticholinesterase (AChE and BChE), antioxidant, and antibacterial activities. While the extract exhibited weak inhibition against AChE (IC50: 507.69 +/- 17.95 mg/mL), it showed relatively greater inhibitory activity against BChE (IC50: 22.41 +/- 0.49 mg/mL). In antioxidant assays, Ult-ER exhibited measurable antioxidant responses, with DPPH and ABTS(+) radical-scavenging capacities of 18.33 +/- 0.49 and 119.59 +/- 1.86 mg TE/L, respectively. Its Fe-3(+) and Cu-2(+) reducing capacities were 128.51 +/- 12.57 and 670.33 +/- 11.59 mg TE/L, respectively. The total phenolic and flavonoid contents were 84.80 +/- 3.31 mg GAE/g and 60.67 +/- 2.29 mg QE/g, while the total antioxidant capacity was 9.78 +/- 0.44 mg TE/g and 15.62 +/- 1.85 mg BHAE/g. In the antibacterial evaluation, Ult-ER at the highest tested concentration of 8 mg/mL exhibited inhibitory activity against all tested strains, including Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853, whereas no detectable inhibition was observed at lower concentrations.
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