Sürdürülebilir Kalkınma Amaçları

Yazarlar

  • MakaleYalnız künye2015Scopus SJRQ2
    Beam dynamics design and electromagnetic analysis of 3 MeV RFQ for TAC proton linac

    Caliskan, A.; Kisoglu, H.F.; Yilmaz, M.

    A beam dynamics design of 352.2 MHz radio-frequency quadrupole (RFQ) of Turkish Accelerator Center (TAC) project which accelerates continuous wave (CW) proton beam with 30 mA current from 50 keV to 3 MeV kinetic energy has been performed in this study. Also, it includes error analysis of the RFQ, in which some fluctuations have been introduced to input beam parameters to see how the output beam parameters are affected, two-dimensional (2-D) and three-dimensional (3-D) electromagnetic structural design of the RFQ to obtain optimum cavity paramaters that agree with the ones of the beam dynamics. The beam dynamics and error analysis of the RFQ have been done by using LIDOS.RFQ. Electromagnetic design parameters have been obtained by using SUPERFISH for 2-D cavity geometry and CST Microwave Studio for 3-D cavity geometry.

    AtıfScopus4WoS3
    ScopusWeb of Scienceİncele
  • MakaleAçık Erişim2015Scopus SJRQ3
    Evaluation of oxidative stress balance in acute deep vein thrombosis; [Akut derin ven trombozunda oksidatif stres dengesinin değerlendirilmesi]

    Menteşe, Ümit; Turan, İbrahim; Doğan, Süheyla; Sümer, Ayşegül; Coşkun, Ceyhun; Alver, Ahmet; Doğan, Orhan Veli; Menteşe, Seda Öztaş; Menteşe, Ahmet

    Objective: The purpose of this study was to show the association between oxidative stress and deep venous thrombosis (DVT) by determining total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) values in patients with DVT and to take precautionary measures to balance oxidative status. Methods: TOS, TAS and OSI levels in serum from 52 individuals with DVT and 45 without DVT were compared. Results: TOS and OSI levels were significantly higher in patients with DVT compared with the control group (p<0.0001), but no difference was observed in TAS levels (p=0.118). The patient group was divided into two subgroups, idiopathic and secondary DVT. TOS and OSI levels were significantly higher patients with both idiopathic DVT and secondary DVT compared to the control group (p<0.0001). TAS levels were significantly higher in patients with idiopathic DVT than in the control group, but no difference was observed in the patients with secondary DVT. Conclusion: Specificity and sensitivity values obtained from the receiver operating characteristic (ROC) curve show that TOS and OSI are both markers that can be used in the diagnosis of DVT. Also, determination of oxidative stress markers may guide clinicians to take necessary precautions to balance oxidative stress in the patients.

    AtıfScopus1WoS1
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  • MakaleYalnız künye2015Scopus SJRQ3
    Determination of the population structure of common bean (Phaseolus vulgaris L.) accessions using lipoxygenase and resistance gene analog markers

    Nemli, Seda; Kutlu, Burcu; Tanyolac, Bahattin

    The common bean (Phaseolus vulgaris L.) is an important food legume throughout the world. Because of the conservation across different plant species, it is possible to evaluate the degree of genetic diversity in the common bean using gene-based marker techniques. The lipoxygenase (LOX) and resistance gene analog (RGA) genes play an important role in the response to biotic and abiotic stresses. Eighty-six common bean accessions were genotyped using gene-based LOX and RGA markers. The total number of polymorphic bands ranged from 193 for LOX to 17 for RGA markers. We detected considerable diversity with a mean of 8.7 alleles per primer for the LOX analysis. For the RGA markers, the number of alleles per polymorphic locus varied from 1 to 4 with an average allele number of 2.8. The genetic similarity between the accessions based on the LOX and RGA markers ranged from 0.12 to 0.55. Using STRUCTURE, 3 groups were revealed among the accessions. The results of this study should provide valuable information for future studies on the genetic diversity of common bean accessions and for association mapping studies examining the relationships between the genotypic and phenotypic traits related to the stress response.

    • SDG 2 · Açlığa Son
    AtıfScopus3WoS3OpenAlex4
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  • MakaleAçık Erişim2015Scopus SJRQ3
    Nutritive value of sunflower silages ensiled with corn or alfalfa at different rate; [Farkli oranlarda yonca veya misir ile kariştirilan ayçiçeği silajinin besin değeri]

    Tan, Mustafa; Yolcu, Halil; Dumlu Gul, Zeynep

    Sunflower (Helianthus annuus L.) having shorter growing season, better cold tolerant and more drought resistant is important alternative silage plant in highlands. But it has poor silage quality. To improve the quality of sunflower silage can be mixed an appropriate amount of corn (Zea mays L.) and alfalfa (Medicago sativa L.). This research was carried out to evaluate quality characteristics of nine silage types: Sunflower, corn and alfalfa silages; plus their some mixtures silages (75% sunflower + 25% corn, 50% sunflower + 50% corn, 25% sunflower + 75% corn, 75% sunflower + 25% alfalfa, 50% sunflower + 50% alfalfa and 25% sunflower + 75% alfalfa); at two harvest stages: Beginning of flowering for sunflower and alfalfa and milk-dough stage for corn (early stage), and end of flowering stage for sunflower and alfalfa, and dough stage for corn (late stage). The experimental design was in a factorial arrangement of randomized complete block with replicated four times. Dry matter content (DM), crude protein content (CP), neutral detergent fiber content (NDF), acid detergent fiber content (ADF), silage pH and physical characteristics (PC) of silages were determined in this study. Mixing of alfalfa in NDF, ADF and CP and corn in DM, pH and PC had positively effect in sunflower silages. Late stage had positively affected DM, PC and pH for silage. Consequently, sunflower silages mixed with corn, with the condition of not being less than 50% at late stage, are recommended for farmers.

    • SDG 2 · Açlığa Son
    AtıfScopus8OpenAlex9
    Scopusİncele
  • MakaleYayıncıda açık erişim2015Scopus SJRQ1
    Inhibition profile of a series of phenolic acids on bovine lactoperoxidase enzyme

    Sarikaya, S. Beyza Ozturk; Sisecioglu, Melda; Cankaya, Murat; Gulcin, Ilhami; Ozdemir, Hasan

    Lactoperoxidase (LPO) catalyzes the oxidation of numerous of organic and inorganic substrates by hydrogen peroxide. It has very vital activity in the innate immune system by decreasing or stopping the activation of the bacteria in milk and mucosal secretions. This study's purpose was to investigate in vitro effect of some phenolic acids (ellagic, gallic, ferulic, caffeic, quercetin, p-coumaric, syringic, catechol and epicatechin) on the purified LPO. This enzyme was purified from milk by using different methods such as Amberlite CG-50 resin, CM-Sephadex C-50 ion-exchange and Sephadex G-100 gel filtration chromatography. LPO was purified 28.7-fold with a yield of 20.03%. We found phenolic acids have inhibition effects on bovine LPO enzyme to different concentrations. Our study showed lower concentrations of caffeic acid, ferulic acid and quercetin exhibited much higher inhibitory effect on enzyme, so these three of them were clearly a more potent inhibitor than the others were. All of compounds were non-competitive inhibitors.

    AtıfScopus42WoS41OpenAlex48
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  • MakaleAçık Erişim2015Scopus SJRQ2
    Cytotoxic effect of Turkish propolis on liver, colon, breast, cervix and prostate cancer cell lines

    Turan, Ibrahim; Demir, Selim; Misir, Sema; Kilinc, Kagan; Mentese, Ahmet; Aliyazicioglu, Yuksel; Deger, Orhan

    Purpose: To investigate the total polyphenolic and flavonoid contents, antioxidant power and cytotoxic activity of ethanol extracts of Turkish propolis (EEP). Methods: The total polyphenolic and flavonoid contents of EEP were determined by spectrometric methods. Antioxidant power and cytotoxic activity of EEP were evaluated using ferric reducing antioxidant power (FRAP) and MTT assays, respectively. Results: The total polyphenolic and flavonoid contents, and FRAP value of EEP were 124.6 ± 1.5 mg gallic acid/g sample dry weight, 42.0 ± 0.8 mg quercetin/g sample dry weight and 311.0 ± 2.5 mg trolox/g sample dry weight, respectively. EEP exhibited powerful cytotoxic effects against the five human cancer cell lines. The highest cytotoxic activity of Turkish EEP was demonstrated on PC-3 cell line (IC50 = 20.7±3.4 μg/mL). Conclusion: The results demonstrate that EEP is a good source of antioxidant and a natural antitumor agent capable of reducing cancer cell proliferation.

    • SDG 3 · Sağlık ve Kaliteli Yaşam
    AtıfScopus64WoS60OpenAlex71
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  • MakaleYalnız künye2015Scopus SJRQ1
    Preliminary assessment of a yoghurt-like product manufactured from hazelnut slurry: Study using response surface methodology

    Ilyasoğlu, Huri; Yilmaz, Firat; Burnaz, Nesibe Arslan; Baltaci, Cemalettin

    The aim of this study was to evaluate the possibility of using hazelnut slurry in manufacture of yoghurt. A yoghurt-like product was prepared from hazelnut slurry fortified with skimmed milk powder. The effects of the total solids content of the hazelnut slurry (TSCHS, 8-16g100g-1) and the content of milk powder (CMP, 6-9g100g-1) on the proximate composition, physicochemical and sensorial properties, fatty acid composition, total phenolic content (TPC) and antioxidant activity of the product were evaluated using response surface methodology. Both the TSCHS and the CMP had a significant effect on the total solids content, b value, syneresis, palmitic and oleic acid content, and FRAP value. Only the TSCHS showed a significant effect on the protein and fat content, a value, water-holding capacity, TPC and ABTS values. Only the CMP showed a significant effect on the carbohydrate and ash content and the acidity. The characteristics of the product generally appeared to be compatible with those of yoghurt. The product was rich in unsaturated fatty acids. Therefore, using hazelnut slurry in manufacture of yoghurt may be proposed to enhance the health benefits of yoghurt.

    • SDG 2 · Açlığa Son
    • SDG 12 · Sorumlu Üretim ve Tüketim
    AtıfScopus24WoS22OpenAlex26
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  • MakaleAçık Erişim2015Scopus SJRQ2
    Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers

    Nemli, Seda; Kianoosh, Tala; Tanyolaç, Muhammed Bahattin

    The common bean (Phaseolus vulgaris L.) is an important crop throughout the world and is highly recommended for many developing countries due to its nutritional components. A retrotransposon-based DNA marker system, interprimer binding sites (iPBSs), developed from reverse transcriptase primer binding sites, was used to determine genetic diversity in common bean accessions. The genetic polymorphism and effectiveness of the marker system were evaluated. A total of 180 polymorphic bands were detected using 47 iPBS primers among 67 common bean accessions, with an average of 4 polymorphic fragments per primer. The genetic similarity between accessions was calculated using the software NTSYS-pc and ranged from 0.09 to 0.99. The average polymorphism information content value for the iPBS markers was 0.73. A model-based clustering method classified the common bean accessions into 4 populations using STRUCTURE software. The results indicated that the iPBS marker method can successfully determine the genetic diversity level in common bean accessions.

    • SDG 2 · Açlığa Son
    AtıfScopus63WoS60OpenAlex56TR Dizin12
    ScopusWeb of ScienceTR Dizinİncele
  • MakaleAçık Erişim2015Scopus SJRQ2
    A comparison of support systems obtained from the RMR89 and RMR14 by numerical analyses: Macka Tunnel project, NE Turkey

    Kanik, Mustafa; Gurocak, Zulfu; Alemdag, Selcuk

    The aim of this study is to highlight the importance of the numerical approach in realistic support design for tunneling. The Macka Tunnel, which is planned to be constructed between the Trabzon-Gumushane highways, located in the Northeastern Turkey, was selected for a field study. The lithology of the study area consists of Late Cretaceous-aged sedimentary rocks, known as the Catak and Esiroglu formations, and the Late Cretaceous-aged Kackar Rhyodacite. In order to determine the geotechnical properties of rock masses and intact rocks, field and laboratory studies were carried out.The rock masses were classified in terms of the Rock Mass Rating (RMR) using the versions of RMR89 and RMR14 and then the preliminary support systems for the tunnel were determined, accordingly. The support systems obtained from the RMR versions were analyzed using the finite element method (FEM). The maximum total displacement and the maximum thickness of plastic zone occurred around the Macka Tunnel after installation the support systems suggested by the RMR89 and RMR14 versions were compared to determine which the RMR version suggests more realistic support system. The results showed that the support systems obtained from the RMR14 version suggests more realistic support element for fair rock masses and great horizontal stress values.

    AtıfScopus29WoS27
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  • MakaleYalnız künye2015Scopus SJRQ1
    Dynamic responses of industrial reinforced concrete chimneys strengthened with fiber-reinforced polymers

    Karaca, Zeki; Türkeli, Erdem; Günaydin, Murat; Adanur, Süleyman

    Industrial reinforced concrete (RC) chimneys are tall and slender structures that need repair and strengthening in their service lives. Fiber-reinforced polymers (FRPs) are continuously becoming a popular material that is used for strengthening purposes of RC structures. The purpose of this study is to determine the effect of FRP strengthening on dynamic responses of industrial RC chimneys. A 75-m-high industrial RC chimney with an opening on the body was selected for FRP strengthening. First, three-dimensional finite element model of the chimney and its modal analyses were performed in ANSYS software. Then, linear transient analyses of the chimney were carried out by using 1992 Erzincan Earthquake ground motion record. After that, body of chimney was strengthened with FRP composite, and dynamic analyses were performed. Finally, dynamic responses of chimney before and after FRP strengthening, such as displacements and maximum-minimum principal stresses, were compared. At the end of the study, it was seen that displacements increased along the height of the chimney, maximum and minimum principal stresses occurred at the region of opening and base for all analysis, respectively. Also, it was seen from this study that FRP strengthening is effective on the dynamic response of industrial RC chimneys.

    • SDG 9 · Sanayi, Yenilikçilik ve Altyapı
    AtıfScopus10WoS9OpenAlex13
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  • MakaleYalnız künye2015Scopus SJRQ2
    Harvestmen fauna of Gümüşhane and Bayburt in Turkey (Arachnida, Opiliones)

    Kurt, Kemal; Erman, Ömer Köksal

    In this study, all samples were collected from Turkey (Gümüşhane and Bayburt provinces) between June 2010 and October 2012. They were evaluated systematically. As a result of these evaluations, 20 species in 14 genera belonging to 4 families were determined. One of them, Rilaena triangularis (Herbst, 1799), is recorded for the first time from Turkey. GPS data of each collecting site, and distribution in Turkey and all over the world of these species are presented in the text.

    AtıfScopus2
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  • MakaleAçık Erişim2015Scopus SJRQ1
    Increased expressions of ADAMTS-13, neuronal nitric oxide synthase, and neurofilament correlate with severity of neuropathology in border disease virus-infected small ruminants

    Dincel, Gungor Cagdas; Kul, Oguz

    Border Disease (BD), caused by Pestivirus from the family Flaviviridae, leads to serious reproductive losses and brain anomalies such as hydranencephaly and cerebellar hypoplasia in aborted fetuses and neonatal lambs. In this report it is aimed to investigate the expression of neuronal nitric oxide synthase (nNOS), A Disintegrin And Metalloprotease with Throm-bospondin type I repeats-13 (ADAMTS-13), and neurofilament (NF) in the brain tissue in small ruminants infected with Border Disease Virus (BDV) and to identify any correlation between hypomyelinogenesis and BD neuropathology. Results of the study revealed that the levels of ADAMTS-13 (p<0.05), nNOS (p<0.05), and NF (p<0.05) were remarkably higher in BDV-infected brain tissue than in the uninfected control. It was suggested that L-arginine-NO synthase pathway is activated after infection by BDV and that the expression of NF and nNOS is associated with the severity of BD. A few studies have focused on ADAMTS-13 expression in the central nervous system, and its function continues to remain unclear. The most prominent finding from our study was that ADAMTS-13, which contain two CUB domains, has two CUB domains and its high expression levels are probably associated with the development of the central nervous system (CNS). The results also clearly indicate that the interaction of ADAMTS-13 and NO may play an important role in the regulation and protection of the CNS microenvironment in neurodegenerative diseases. In addition, NF expression might indicate the progress of the disease. To the best of the authors' knowledge, this is the first report on ADAMTS-13 expression in the CNS of BDV-infected small ruminants.

    • SDG 3 · Sağlık ve Kaliteli Yaşam
    AtıfScopus15WoS14OpenAlex15
    ScopusWeb of SciencePubMedİncele
  • MakaleAçık Erişim2015Scopus SJRQ2
    Utilization of the limit equilibrium and finite element methods for the stability analysis of the slope debris: An example of the Kalebasi District (NE Turkey)

    Alemdag, Selcuk; Kaya, Ayberk; Karadag, Mustafa; Gurocak, Zulfu; Bulut, Fikri

    The stability of the slope debris in residential area of the Kalebasi District (Ozkurtun-Gumushane) was investigated using the Limit Equilibrium (LE) and Finite Element Shear-Strength Reduction (FE-SSR) methods. Along the survey lines, four trial pits were dug and fourteen boreholes having a total length of 345m were drilled. Also, seismic refraction studies were conducted along the five lines. According to the field studies, thickness of the slope debris covering the 98ha of the study area varies between 1 and 36m. To determine the physical and shear strength properties of the slope debris, undisturbed samples were taken from the trial pits. As a result of the laboratory tests, soil categories of the debris were found to be as Clayey Sand (SC), Silty Sand (SM) and Low Plasticity Clay (CL). The deformation-controlled shear box tests were carried out to determine the shear strength parameters of the slope debris. According to these tests it was found that the peak cohesion and peak friction angle varies between 2.63-16.35kN/m2 and 20-27°, respectively. Stability analyses were performed using the obtained data from field and laboratory investigations in the Slide v5.0 and Phase2 v6.0 software programs and results were compared. In LE stability analyses, the factor of safety (FOS) of survey lines were found to be as 1.44, 1.80, 1.96, and 1.72; however for the FE-SSR method they were determined as 1.39, 1.72, 1.59, and 1.58, respectively.

    AtıfScopus42WoS36
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  • MakaleAçık Erişim2015Scopus SJRQ1
    N-Acylsulfonamides strongly inhibit human carbonic anhydrase isoenzymes i and II

    Yildirim, Alper; Atmaca, Ufuk; Keskin, Ali; Topal, Meryem; Çelik, Murat; Gülçin, Ilhami; Supuran, Claudiu T.

    Sulfonamides represent a significant class of biologically active compounds that inhibit carbonic anhydrase (CA, EC.: 4.2.1.1) isoenzymes involved in different pathological and physiological events. Sulfonamide CA inhibitors are used therapeutically as diuretic, antiglaucoma, antiobesity and anticancer agents. A series of new sulfonamides were synthesized using imides and tosyl chloride as starting materials. These N-acylsulfonamides efficiently inhibited the cytosolic human carbonic anhydrase isoenzymes I, and II (hCA I, and II), with nanomolar range inhibition constants ranging between 36.4 ± 6.0-254.6 ± 18.0 and 58.3 ± 0.6-273.3 ± 2.5 nM, respectively.

    AtıfScopus158WoS155
    ScopusWeb of SciencePubMedİncele
  • MakaleAçık Erişim2015Scopus SJRQ2
    An Experimental Study on Heat Transfer and Pressure Drop of CuO-Water Nanofluid

    Sahin, Bayram; Manay, Eyuphan; Akyurek, Eda Feyza

    Heat transfer and pressure drop characteristics of water based CuO nanofluid inside a horizontal tube were investigated experimentally. The upper limitation of the particle volume fraction with respect to heat transfer performance was also found. CuO-water nanofluids with volume fractions of 0.5%, 1%, 2%, and 4% were prepared by dispersing the CuO nanoparticles with an average diameter of 33 nm into deionised water. Experiments were carried out under the steady-state, constant heat flux, and turbulent flow regime conditions. The variations of the average Nusselt number and the friction factor with the Reynolds number were presented. For all given particle volume concentrations, heat transfer enhancements were calculated. It was concluded that the particle volume concentrations higher than 1% vol. were not appropriate with respect to the heat transfer performance of the CuO-water nanofluid. No heat transfer enhancement was observed at Re = 4.000. The highest heat transfer enhancement was achieved at Re = 16.000 and = 0.005.

    AtıfScopus35WoS29OpenAlex35
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  • MakaleAçık Erişim2015Scopus SJRQ1
    Changes in sterol composition of hazelnut during fruit development

    Ilyasoglu, Huri

    Hazelnut is a valuable source of fat-soluble bioactive components. Among the fat-soluble bioactive components, phytosterols have gained much interest due to their cholesterol reducing properties. This study presents the evaluation of changes in the sterol composition of Turkish hazelnut cultivars (Tombul, Palaz, and Sivri) during fruit development. Three major sterols (β-sitosterol, campesterol, and Δ5-avenasterol), and five minor sterols (clerosterol, brassicasterol, stigmasterol, Δ7-stigmastenol, and Δ7-avenasterol) were detected in all hazelnut cultivars during all stages. The total sterol contents of Turkish hazelnut cultivars reached the maximum values at the early stage and a significant reduction in the total sterol content occurred at the harvest stage.

    • SDG 2 · Açlığa Son
    AtıfScopus6WoS6OpenAlex4
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  • MakaleYalnız künye2015Scopus SJRQ2
    Biogeochemical characteristics of Rosa canina grown in hydrothermally contaminated soils of the Gümüşhane Province, Northeast Turkey

    Vural, Alaaddin

    Kırkpavli alteration area (Gümüşhane, Northeast Turkey) is contaminated by heavy metals such as Cd, Pb, As, Cu and Zn. The quantity of accumulation of heavy metal trace elements and macroelements in 32 leaves of Rosa canina of the Kırkpavli alteration area has been studied within the scope of geochemical studies. Element contents of samples were assessed using various parameters including descriptive statistics, factor analysis, correlation coefficients and bioaccumulation factor. Concentrations were detected in the acceptable range for Mo, Cu, Pb, Ni, As, Cd, Sb, P, Ti, Na, Se and Sn. Concentrations of Co, Mn, Ba and Hg were detected close to the acceptable values, whereas Zn, Fe, Sr, V, Ca, Cr, Mg, B, Al, K, W, Sc, Cs and Rb concentrations were detected above the acceptable values. Principal component analysis was used to identify the elements that have a close relationship with each other and/or similar origins. It has been concluded that Zn, Cu, As and Mo content of the plant were related to hydrothermal alteration process and they behaved together, whereas Mn and Fe were especially products of weathering conditions, also behaved together. In terms of macroelements, Ca, Mg and Na had similar behaviour, while P and K had the same correlation.

    • SDG 2 · Açlığa Son
    • SDG 15 · Karasal Yaşam
    AtıfScopus16WoS16OpenAlex21
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  • MakaleYalnız künye2015Scopus SJRQ4
    Synthesis, antifungal activity and carbonic anhydrase inhibitory properties of Cu (II) Bis (3, 4 dimethoxybenzoate) bis (nicotinamide) dihydrate

    Afsin Kaya, A.; Kaya, Elif Celenk; Sarikaya, S. Beyza Ozturk; Onaran, Abdurrahman

    The new 3,4-dimethoxybenzoate complex of Cu(II) with nicotinamide was synthesized and characterized by elemental analysis, FT-IR spectroscopic study, UV-Vis spectrophotometry and mass spectrometry. The thermal behavior of the complex was studied by simultaneous TG, DTG and DTA methods in static air atmosphere. The infrared spectral characteristics of the complex is also discussed. In this complex, all ligands are coordinated to the metal ion as monodendate ligands. The final decomposition products were found to be the respective metal oxide. As it is well known, metal complexes of biologically important ligands are sometimes more effective than the free ligands. In this study the new compound was synthesized due to its functional groups such as veratric acid and nikotin amide, which have known biological characteristics and effective in terms of both enzyme and antifungal properties from recent studies. Therefore, antifulgal activity and enzyme inhibition properties of this complex have been examined.

    AtıfScopus3OpenAlex3
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  • MakaleYalnız künye2015Scopus SJRQ3
    A note on weighted Sobolev algebras L1ω(ℝd) ∩ Wpk (ℝd)

    Güngör, Nihan; Səgir, Birsen

    Let 1 ≤ p < ∞ and ω be Beurling's weight function on ℝd. In this article, we demonstrate some harmonic properties of Sobolev space Wpk (ℝd) which consists of all functions on ℝd whose weak derivatives up to and including order k belong to Lp(ℝd). Also, we deal with some harmonic properties of intersection space Apk,ω(ℝd) = L1ω(ℝd) ∩ Wpk (ℝd) by aid of Beurling algebra L1ω(ℝd) and Sobolev space Wpk (ℝd). Finally, we research the inclusions and continuous embeddings between the spaces Apk,ω(Ω) where Ω ⊂ ℝd be open set.

  • MakaleYalnız künye2015Scopus SJRQ3
    Purification and characterization of an extracellular cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey

    Genc, Berna; Nadaroglu, Hayrunnisa; Adiguzel, Ahmet; Baltaci, Ozkan

    In the present study, cellulase was purified and characterized from Anoxybacillus gonensis (Gen bank Number: KM596794) which was isolated and characterized from Agri Diyadin Hot spring. It was found to synthesize cellulase which had a wide range of industrial applications. Twenty four-hour-cultured bacteria induced cellulase production and specific activities during the purification steps were 1.47,81.06 and 109.4 EU mg-1 protein at crude extract, ammonium sulphate precipitated and DEAE-Sephadex purification steps. The highest enzyme activity was observed at 50°C and the optimum range of pH was 3-10. Molecular weight of enzyme was determined approximately 40kDa. The kinetic parameters of cellulase against carboxymethylcellulose (CMC) were 153.4 μmol min-1 mg for Vmax and 0.46mM for Km. Among effectors of the enzyme, Zn2+, Ca2+, Co2+ and EDTA decreased enzyme activity.

    AtıfScopus18WoS14
    ScopusWeb of SciencePubMedİncele