Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Kaygusuz, Abdullah(35)
- Sipahi, Ferkan(35)
- Karsli, Orhan(34)
- Dokuz, Abdurrahman(30)
- Duran, Celal(30)
- Kandemir, Raif(29)
- Ozdes, Duygu(28)
- Maden, Nafiz(27)
- Uzun, Sevda(27)
- Merdan, Mehmet(26)
- Vural, Alaaddin(26)
- Öztürk, Serkan(25)
- Araz, Onur(24)
- Kulakaç, Nurşen(24)
- Ashyralyyev, Charyyar(23)
- Yildiz, Salih(23)
- Yilmaz, Ozgur(23)
- Doğan, Kadir Caner(21)
- Arslan, Mehmet(20)
- Aydin, Faruk(20)
- Doğru, Bülent(20)
- Bayraktar, Alemdar(19)
- Kankal, Murat(19)
- Ayaydin, Hasan(18)
- Baltaci, Cemalettin(18)
- Yolcu, Halil(18)
- Cakir, Tufan(17)
- Cengiz, Ekrem(17)
- Demir, Selim(17)
- Gücer, Mehmet Ali(17)
- Akaryali, Enver(16)
- Artan, Seyfettin(16)
- Hayaloğlu, Pınar(16)
- Kaya, Afşin Ahmet(16)
- Tosunoğlu, Büşra(16)
- Uysal, Ibrahim(16)
- Kurt, Kemal(15)
- Ozturk, Samet(15)
- Reis, Hatice Catal(15)
- Aksakal, Vecihi(14)
Yayınlar
- MakaleYalnız künye2021Scopus SJRQ3Long and short-term assessment of surface area changes in saline and freshwater lakes via remote sensing
Yagmur, Nur; Bilgilioglu, Burhan Baha; Dervisoglu, Adalet; Musaoglu, Nebiye; Tanik, Aysegul
Advances in remote sensing technology enable monitoring and detection of these vulnerable water bodies that bear numerous functions and ecological services beyond their intended use. As such, valuable data and information may be provided for long and short-term analyses and stored in a database for future projections. This paper initially grouped 18 natural lakes of the Konya Closed Basin of Turkey according to their salinity level. Freshwater, saline and brackish lakes have then undergone temporal analysis for every 5-year intervals via Landsat satellite images. Freshwater lake surfaces have not changed noticeably during the inspection period; however, saline waters have decreased in surface area by 32%. Beysehir Lake constituting 94% of the freshwater category and Tuz Lake representing 97% of the saline group were selected for the short-term analysis that was monthly conducted for years 2017 and 2018 with optical and SAR images to better verify cause and effect relationship.
AtıfScopus13WoS11OpenAlex16
İncele - MakaleYalnız künye2021Scopus SJRQ3The performance of wong-zakai approximations for the investigation of stochastic differential equation models with nonlinear multiplicative noise
Sengul, S.; Bekiryazici, Z.; Merdan, M.
In this study, Wong-Zakai approximation method has been applied for the analysis of stochastic differential equations appearing in engineering sciences. Wong-Zakai approximation has been used with similar stochastic approximation methods to compare the approximate solutions of the problems and comment on the performance of the method. Models for lake pollution and computer virus spread under antivirus protection have been used with nonlinear stochastic noise as numerical examples to demonstrate the efficiency of Wong-Zakai method.
- MakaleYalnız künye2021Scopus SJRQ1Effects of puzzolanic materials in surface paste disposal by pilot-scale tests: observation of physical changes
Bascetin, A.; Adiguzel, D.; Eker, H.; Odabas, E.; Tuylu, S.
There are only a few pilot schemes, especially among scientific studies carried out to solve numerous environmental and ecological problems created by water during tailing storage on the surface. It is extremely important to establish precautions that can be taken, especially in the tailing storage process by examining the physical, chemical, mineralogical, and geotechnical changes that develop due to the effect of moisture in the tailing and surface water. In this study, 2 different pilot-scale designs of storage with no additions (reference) and with binder additions (cement and fly ash) to some layers were observed in field conditions for about 1 year to determine the effects of seasonal changes on paste material. The results of the measurements revealed that the water content of the reference design was ~ 10% higher than that of the design with cement addition at the substrates that provide stability and sudden temperature changes at the top layer of the reference design was found to be ~ 35 °C. Maximum sudden temperature changes in layers with cement addition and layers with cement and fly ash addition were observed to be ~ 15 °C. As a result, the use of cement has made the material more resistant to external factors. However, the study also revealed that fly ash could be used instead of cement with a substitute rate of 50% to reduce the cost for long-term storage in terms of industrial applications.
AtıfScopus22WoS17OpenAlex38İncele - MakaleYalnız künye2021Scopus SJRQ3The effect of homocysteine on the expression of CD36, PPARγ, and C/EBPα in adipose tissue of normal and obese mice
Mentese, Ahmet; Dogramaci, Seniz; Demir, Selim; Yaman, Serap Ozer; Ince, Imran; Altay, Diler Us; Erdem, Mehmet; Turan, Ibrahim; Alver, Ahmet
The purpose of this study was to investigate the effect of homocysteine (Hcy) on CD36, PPARγ, and C/EBPα gene and protein expression in adipose tissue obtained from normal and high-calorie diet obesity models. CD36, PPARγ, and C/EBPα gene expression and protein levels in adipose tissue specimens were determined using the RT-PCR and ELISA methods, respectively. Significantly increased CD36 gene expression was observed in adipose tissue from obese mice, while Hcy significantly reduced CD36 gene expression in adipose tissue from normal and obese mice. PPARγ and C/EBPα gene expression levels decreased significantly in all groups compared to the normal group. In addition, levels of both PPARγ and C/EBPα gene expression were lower with Hcy supplementation compared to their own controls. In conclusion, Hcy’s reduction of CD36 gene expression in adipose tissue may be one probable factor in hyperhomocysteinemia representing an independent risk factor for cardiovascular diseases.
AtıfScopus2WoS2OpenAlex2
İncele - MakaleYalnız künye2021Scopus SJRQ1Sodium silicate effect on setting properties, strength behavior and microstructure of cemented coal fly ash backfill
Cavusoglu, Ibrahim; Yilmaz, Erol; Yilmaz, Ali Osman
Representing an engineered mixture of coal fly ash, cement and water, cemented coal fly ash backfill (CCB) is typically employed for filling the openings created after coal excavation in the earth. A high rate of productivity for coal mines requires a high early-age strength backfill design. Therefore, the use of admixtures in CCB is key for accelerating the backfill setting time and augmenting short-and long-term strength. This study offers the effect of sodium silicate (SS) as an accelerator on early age mechanical and microstructure properties of CCB. SS-based admixture was used for a dosage of 0.5, 1 and 1.5 wt% in CCB that contains 25 wt% ordinary Portland cement OPC and 75 wt% coal fly ash. Backfill setting time was done by using the Vicat system while its strength and pore structure were measured by using the uniaxial compressive strength test, and scanning electron microscopy and Hg intrusion porosimetry. Results display that use of SS in CCB increases its short-term strength gain up to 14% and shortens the mining cycle by cutting its setting time up to 58%. The microstructure analyses also indicate that CCB formulated with SS has a finer pore structure than that without admixture. The outcomes of the present study will be advantageous to evaluate both setting time and strength behavior of CCB used in coal mines, thereby causing the effective backfill plan in terms of money saving and easy operation.
AtıfScopus149WoS141OpenAlex146İncele - Kitap BölümüYalnız künye2021Integrated Reporting and Combined Assurance: A Qualitative Research on the Awareness in Turkey*
Selimoglu, Seval Kardes; Yesilcelebi, Gul
Purpose: The aim of this study is to reveal the opinions of the auditors, academicians, and institutions that published integrated reports regarding the development and execution of the assurance process of integrated reports. Design/methodology/approach: For this purpose, interviews were conducted using qualitative research technique to determine awareness about integrated reporting and combined assurance. Within the scope of the research, semi-structured interviews were conducted with six auditors, five academicians, and five workers in institutions that published integrated reports. Qualitative data analysis method was used to analyze the data. Findings: As a result of the research, combined assurance process criteria were proposed in the integrated reports which in line with the opinions of the participants. Originality/value: Institutions around the world are increasingly publishing integrated reports. However, when institutions publish integrated reports, there is no clear standard or any guidance on how to ensure the reliability of these reports. It is seen that AA1000, ISAE3000, GRI Standards, and some local standards are used to provide assurance. At this point, the combined assurance model can be used for the reliability of the information in the integrated reports. Integrated reporting and combined assurance are still relatively new concepts in Turkey. Furthermore, this study is important in terms of the lack of studies on how to provide combined assurance for integrated report when scanned related literature in Turkey. Although readily integrated reporting continued in Turkey, it continues to be an area of application is still under development. In particular, the research reflects the level of integrated reporting awareness and how to ensure assurance of these reports.
AtıfScopus1OpenAlex3İncele - MakaleYalnız künye2021Scopus SJRQ1A reduced variance unsupervised ensemble learning algorithm based on modern portfolio theory
Ünlü, Ramazan; Xanthopoulos, Petros
Unsupervised ensemble learning or consensus clustering has gained popularity due to its ability to combine multiple clustering solutions into a single solution that is robust and often performs better than the individual ones. There have been several approaches to consensus clustering including voting and weighted voting algorithmic schemes. Although there have been several algorithms for adjusting the weights of a consensus clustering all of them are tuned based on some performance characteristic associated with clustering accuracy. In this paper, we propose a method for incorporating weights by taking into consideration the intra algorithmic variability i.e. algorithms that provide solutions with very different performance upon multiple runs. The methodology is inspired by modern portfolio theory and more specifically from Markowitz model for asset allocation where one is trying to identify the most efficient portfolio through the solution of a convex optimization problem. Here, efficiency is defined as the minimum amount of risk for an expected return. We apply this method to different datasets and compare with respect to performance and robustness. The proposed scheme appears to achieve competitive average performance with very low variability.
AtıfScopus13WoS7OpenAlex15İncele - MakaleYalnız künye2021The Relationship between Body Mass Index and Eating Disorder Risk and Intuitive Eating among Young Adults
Yılmaz, Hacı Ömer; Zemzemoglu, Tuba Eda Arpa
Background: Obesity is a serious health problem that threatens public health. There are limited researches exploring the relationship between obesity and eating disorder risk and intuitive eating. The purpose of this study was to determine the rate of eating disorder risk and obesity, and identify the relationship between body mass index (BMI) and eating disorder risk and intuitive eating among young adults. Methods: This cross-sectional study enrolled 1216 young adults. The data were obtained through a questionnaire consisted of three sections of socio-demographic information in the first part, Intuitive Eating Scale-2 (IES-2) in second part and Eating Attitudes Test-26 (EAT-26) in third part. Weight, height, and BMI of participants were also measured by researchers. Results: The overall overweight/obesity rate of the participants was 16% (male=25.9%; female=10.9%). The rate of eating disorders risk was 24.3% in general (male=21.5%; female=25.4%). Overweight/obese had higher eating disorders risk than normal and underweight (26.7%, 23.8%, 21.6%, respectively). IES-2 score of underweight was significantly higher than overweight/obese (3.33±0.35, 3.20±0.37, respectively). Conclusion: This is the first study to examine the relationship between BMI and EAT-26 subscale showing that BMI was positively correlated with EAT-26 and negatively was correlated with IES-2 score. Therefore, BMI may be affected by eating behaviors and intuitive eating. Also, intuitive eating can be a new method of eating, and weight control and management.
AtıfScopus11OpenAlex7İncele - Kitap BölümüYalnız künye2021Pandemic transaction cost and impact on the economy
Çiçen, Yildirim Beyazit
- MakaleYalnız künye2021Scopus SJRQ1A rapid method to detect green pea and peanut adulteration in pistachio by using portable FT-MIR and FT-NIR spectroscopy combined with chemometrics
Aykas, Didem Peren; Menevseoglu, Ahmed
In this research, the determination of green pea and peanut adulteration in pistachio by portable Fourier transform mid-infrared (FT-MIR) and Fourier transform near-infrared (FT-NIR) spectroscopy was studied. A rapid method to detect adulteration in pistachio is a necessity in the food industry because of its high commodity value and being one of the most consumed tree nuts. Pistachio nut is a target for economically motivated adulteration, and green pea, spinach, and peanut are the most seen adulterants in pistachio. Portable FT-MIR and FT-NIR spectrometers are simple, non-invasive, rapid, easy to transport, and cheaper alternatives to traditional methods, including chromatography (GC, HPLC), mass-spectroscopy (GC-MS, LC-MS) for the detection of pistachio adulteration. Powdered pistachio samples were adulterated with powdered green pea and peanut samples at different concentrations (5–40%). Spectra were collected with a portable FT-MIR and FT-NIR spectrometers and analyzed by Soft Independent Modeling of Class Analogy (SIMCA) to generate a classification model to authenticate pure pistachio, and Partial Least Square Regression (PLSR) to predict the levels of adulterants in pistachio. SIMCA provided, in both units, very distinct clusters for pure and adulterated samples with inter-class distance (ICD) over 3. Both units also showed superior performance in predicting adulterant levels with rVal>0.99, standard error of prediction (SEP) < 2.5%. Based on SIMCA and PLSR models, portable FT-NIR spectrometer provided more advantages over portable FT-MIR unit because the prediction with the FT-NIR spectrometer was more precise than the FT-MIR unit. Both portable FT-MIR and FT-NIR units can be used as alternative methods to traditional methods and showed great potential for real-time surveillance to detect green pea and peanut adulteration in pistachio.
AtıfScopus70WoS60OpenAlex71İncele - MakaleYalnız künye2021Scopus SJRQ2Determining the phenolic components by using HPLC and biological activity of Centaurea triumfetti
Acet, Tuba
Centaurea species belonging to Asteraceae family are traditionally used herbs in treating many diseases in Anatolia. In the present study, I aimed to determine the biological activities and phenolic contents of the extracts of the aerial parts of Centaurea triumfetti collected from areas with extreme climatic conditions. The determination of antioxidant activity was performed using in vitro assays such as ABTS (2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (1,1-diphenyl-2-picrylhydrazyl). Disc diffusion and microdilution methods were used for determine of antimicrobial activity. Besides that, the diabetes-related inhibition activities of enzymes such as alpha-glucosidase and alpha-amylase were determined using colorimetric methods. The phenolic components of the extracts were analysed by using HPLC. The biological activity of ethyl acetate extracts of the stem was found to be higher those than that of other extracts. This extract was also found to have high antimicrobial and enzyme inhibition activity. Moreover, the major component of the extract was found to be chlorogenic acid. In conclusion, these results suggest that C. triumfetti has the potential for pharmaceutical usage.
AtıfScopus18WoS21OpenAlex17İncele - MakaleYalnız künye2021Scopus SJRQ1Additivity effect on properties of cemented coal fly ash backfill containing water-reducing admixtures
Cavusoglu, Ibrahim; Yilmaz, Erol; Yilmaz, Ali Osman
Cemented coal fly ash backfill (CCB), a flowable structure of coal fly ash, binding agent and mixing water, has been often employed in mining operations to offer ground support as well as waste disposal solutions. An appropriate CCB mass should have sufficient rheological (e.g., workability) and mechanical (e.g., strength and stability) characteristics. Chemical admixtures are well known to greatly enhance the pumpability and strength properties of cementitious materials. Thus, this work provides the effect of water-reducing admixtures (namely, Sikament MR 50 W and Sikament FFN) on characteristics of CCB materials manufactured at a constant slump value. The role of admixtures in CCB mix is to reduce the cement-related costs, to modify the properties of the hardened backfills, and to ensure the quality of the backfill during mixing, transporting, placing, and curing. These admixtures were used at a dosage of 1% and 2% by weight of anhydrous material on cement. Several laboratory tests including slump, setting time, uniaxial compressive strength, scanning electron microscopy and mercury intrusion porosimetry were undertaken on CCB samples to understand their fresh and hardened performance. Experimental results show that the admixtures used ensure the slump required to deliver CCB at low water/cement ratios, improve short- and long-term backfill strengths up to 33%, and decrease the total porosity of the tested CCB. The findings of this study also show that the admixture use in CCB leads to a significant decrease in the considered cement content and a remarkable increase in the mechanical strength. As a result, understanding the actual fresh and hardened properties of CCB remains crucial for the choice of the greatest acting backfill admixture and for a fruitful change from capable experimental results to the in-situ conditions.
AtıfScopus76WoS69OpenAlex78İncele - MakaleYalnız künye2021Scopus SJRQ1Particle Erosion Performance of Additive Manufactured 316L Stainless Steel Materials
Azakli, Zeki; Gümrük, Recep
Many critical parts such as surface parts of high-speed trains, bins and hoppers, and pipes, bends and valves in material transportation applications as well as a helicopter, mixer, and turbine blades, which can be exposed to particle wear due to the application environment, can be easily manufactured today due to the advances in 3D printing technology. For this reason, an experimental study was carried out to determine the particle erosion behavior of 316L stainless steel parts built by the laser melting method, which is one of the 3D printing technologies. The rectangular plate samples produced with various laser manufacturing parameters were subjected to particle erosion tests at different impingement angles in accordance with the ASTM G76 standard at 140 m/s impact speed. The results showed that the erosion behavior develops in a characteristic similar to the wrought sample, and laser parameters play an efficient role. For example, the porosity ratio decreases at low scanning speeds, resulting in significant improvements in erosion behavior. Also, it was found that the particle erosion behavior of the 3D printed parts could be improved in a serious amount by applying a heat treatment process. Consequently, the obtained results in this study promotes the more comprehensive works regarding the optimization of several laser manufacturing parameters such as scan speed, build direction, laser power, hatch spacing, layer thickness, scan strategy and laser exposure time to obtain the best particle erosion behavior and determination of the most suitable materials among the several others which can be used in 3D printing.
AtıfScopus19WoS17OpenAlex17İncele - MakaleYalnız künye2021Scopus SJRQ3Determination of the Chemical Composition, Antioxidant and Antimicrobial Activities of Different Parts of Rosa canina L. and Rosa pimpinellifolia L. Essential Oils
Öz, Mehmet; Deniz, Ilhan; Okan, Onur Tolga; Baltaci, Cemalettin; Karatas, Seyda Merve
Today, the use of essential oils is increasing day by day, and it is considered as raw materials in many industries. Therefore, this study aims to identify the essential oil profile, antioxidant and antimicrobial activity of Rosa canina L. and Rosa pimpinellifolia L. reveal their potential use in the industry. Essential oils that were obtained in 2013-2014 from different parts (flower, leaf, stem, and fruit) of Rosa canina L. and Rosa pimpinellifolia L. by the hydro-distillation method were analyzed by GC/MS. The dominant components in Rosa canina L. were found as trans-geraniol in the flowers (42.08 % for 2013), linalool in the leaves (11.55 % for 2014), and stem (14.91 % for 2013) and vitispirane in the fruits (37.19 % for 2014). The dominant components in R. pimpinellifolia L. were found as heneicosane in the flowers (22.07 % for 2014), γ-elemene in the leaves (30.21 % for 2013), linalool in the stem (14.76 % for 2013) and the fruits, (E)-2-hexenal for those collected in 2013 (14.09 %). The IC50 and FRAP values of the samples ranged from 127.54 to 142.22 μg/g and 121.43 to 296.78 μmol TE/g, respectively. According to the results obtained, both R. canina L. and R. pimpinellifolia L. were found to show poor antioxidant values of DPPH and FRAP. In general, it was determined that the essential oils obtained from different plant parts of both species had a moderate antimicrobial effect against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, and Pseudomonas aeruginosa.
AtıfScopus17WoS16İncele - MakaleYalnız künye2021Scopus SJRQ4Chemical composition and biological activities of volatile oils of arabis alpina l. Ssp. alpina
Ucuncu, O.
This work reports volatile constituents, antimicrobial and antioxidant activity of volatile oils from the air-dried flower and aerial parts (APs) of Arabis alpina L. ssp. alpina. The volatile components of flower and aerial parts (stem + leaf) were investigated by means of hydrodistillation in Clevenger type apparatus and GC/MS/FID analysis. Fifty-one and fifty-three compounds in the volatile oils of flower and APs of A. alpina were identified. Terpene derivatives were the major volatiles in both oils. Hexahydrofarnesyl acetone was the main component of flower oil and APs oil in ratios of 16.27% and 26.94%, respectively. Additionally, both flower and APs essential oils were investigated for their antimicrobial activity against twelve bacteria and five fungi, using agar dilution method and antioxidant activities by using DPPH˙, ABTS˙+ and Folin-Ciocalteu assays. Flower oil was effective against B. subtilis and B. cereus even at 100µg/mL. The amount of total phenolic, %DPPH˙ scavenging activity and ABTS˙+ scavenging activity were found as 485.60±7.28 mg/mL GAE, 49.85±1.22% and 166.43±12.05µM Trolox equivalent, respectively. Flower oil exhibited antimicrobial effect against gram positive bacteria especially, and moderate antioxidant activity.
AtıfScopus3WoS2İncele - Kitap BölümüYalnız künye2021Environmental Policies, Climate Change Perception and Trust
Ugur, Candan Yılmaz
- MakaleYalnız künye2021Scopus SJRQ1The Effect of Progressive Muscle Relaxation Exercises on Pain on Patients with Sickle Cell Disease: Randomized Controlled Study
Kazak, Aysun; Ozkaraman, Ayse
Purpose: The study aimed to evaluate the effect of progressive muscle relaxation exercises on pain in patients with sickle cell disease. Materials-Method: This randomized controlled interventional study was conducted in a hospital in Mersin, Turkey between October 2017 and July 2018. The study sample comprised 58 patients who were aged >18 years, conscious, had sickle cell disease, reported pain, and were treated with non-opioid or weak opioid analgesic based on the physician's recommendation (treatment group = 29, control group = 29). Data were collected by the individual presentation form and visual analog scale. During the study, both groups were treated with analgesics prescribed by the physician for three days. In addition to the analgesics, the treatment group was performed progressive muscle relaxation exercises for 30 minutes whereas the control group was rested. Pain level of both groups was evaluated at three time points every day for three days. In the study, frequency distributions and descriptive statistics were presented for categorical and numerical variables, respectively. Chi-square analysis, independent samples t-test, Mann–Whitney U test, three-way ANOVA, and Sidak test were used to analyze the difference between the variables. Results: The mean age of the patients was 29.59 ± 6.94 years, and 53.4% of the patients were female and 69% were single. The mean pain score of the treatment group at the third time point on days 1, 2, and 3 was significantly lower than the control group (p <.05). There was no statistically significant difference between the two groups in terms of coping methods for pain, pain location, and complaints accompanying pain (p >.05). Conclusion: Progressive muscle relaxation exercises were found to be effective in the pain management of patients with sickle cell anemia.
AtıfScopus7WoS5OpenAlex16İncele - Kitap BölümüYalnız künye2021Chingiz Aitmatov's grand narrative
Çakin, Ömer; Günay, Mehmet Akif
Beyond political and cultural criticisms, grand narratives have the authority to disclose real facts. This authority is most effective in the field of culture. Grand narratives are the universal presentation of local culture. In a culture dominated by grand narratives, the dominant ideology of the regime is to control knowledge. Grand narratives play a role in generalizing and transferring information skillfully while describing the contemporary situation. A novelist was defined as a mirror reflecting the period and society in which he lived. Likewise, Chingiz Aitmatov tried to depict the political, social, and cultural structure of his period, and accordingly, formed his own grand narrative. The author skillfully revealed the facts of the period regarding the Marxist tradition and described the social, cultural, and political structure of the period using a tentative language in parallel with the facts such as analogy, myth, and legend.
AtıfScopus1İncele - MakaleYalnız künye2021Contribution to the taxonomic knowledge of acanthodactylus (Squamata, lacertidae): Description of a new lacertid lizard species from eastern anatolia, turkey
Kurnaz, Muammer; Şahin, Mehmet Kürşat
Acanthodactylus Wiegmann, 1834 is one of the most diverse and widespread lizard genus in the Palearctic realm. Here, we describe a new species, - Acanthodactylus ilgazi sp. nov. - from the Anatolian Peninsula. This new species ranges approximately 250 km north from the closest population of this genus in Turkey. Compared to other fringe-fingered lizards, the new species is phylogenetically close to A. robustus, A. tristrami and A. orientalis but it has some distinct morphological characteristics: Reddish coloration under the tail, a sharp white or grayish stripe in the middle of the dorsum, and four plates in a row on the 4th finger. Moreover, phylogenetic molecular data, based on cyt b gene fragment, verifies that the new species is phylogenetically a member of the tristrami species group with 13.03%, 17.35% and 20.56 genetic distance respectively from A. orientalis, A. tristrami and A. robustus. Lastly, the known range of this species, located in Yazıhan, Malatya in Eastern Anatolia, is restricted by a dam, thus habitat loss endangers its continuity. Therefore, the conservation status of this species should be assessed immediately.
AtıfScopus6İncele - MakaleYalnız künye2021Scopus SJRQ2From Turkey: First Report of KPC-3-and CTX-M-27-Producing Multidrug-Resistant Klebsiella pneumoniae ST147 Clone Carrying OmpK36 and Ompk37 Porin Mutations
Özad Düzgün, Azer
Aim: The aim of this study was to identify antimicrobial resistance genes, virulence factor genes, and porin loss or mutations exhibited by the multidrug-resistant Klebsiella pneumoniae strain. Materials and Methods: Whole-genome sequencing was done via the Illumina NovaSeq 6000 platform. Strain identification and antibiotic susceptibility testing of strains were performed by the Vitek 2 automated system. Multilocus sequence typing analysis was carried out using seven conserved housekeeping genes. Results: The strain was resistant to penicillins, cephalosporins, carbapenems, aminoglycosides, fluoroquinolones, fosfomycin, and trimethoprim/sulfamethoxazole. The isolate was found to carry KPC-3, CTX-M-27, SHV-11, SHV-67, and TEM-1 β-lactamases. The clonal subtype of the isolate was ST147, and it possessed wzi64 and wzc38 alleles. Fifteen different point mutations (N49S, L59V, R146H, V178P, G189T, F198Y, V202L, F207Y, A217S, T222L, D223G, H235N, A280V, N304E, and S346N) were detected in the OmpK36 porin. A frame shift was observed in OmpK35 and two different point mutations (I70M and I128M) were found in the OmpK37 porin, in addition to seven mutations observed on the AcrR. Conclusions: This study demonstrated for the first time that the ST147 clone produced CTX-M-27 as well as KPC-3. In addition, new mutations were detected in the outer membrane proteins. These mutations together with the production of extended-spectrum β-lactamase and carbapenemase were found to contribute to the resistance of the ST147 clone to carbapenem and other antibiotics.
AtıfScopus11WoS11OpenAlex9İncele