Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Akar, Zeynep(4)
- Akmeşe, Osman(3)
- Aliyazicioglu, Yüksel(2)
- Aygül, İmdat(2)
- Can, Zehra(2)
- Demir, Selim(2)
- Fidan, Muhammed Said(2)
- Gültekin, Ergün(2)
- Turan, İbrahim(2)
- Öz, Mehmet(2)
- AKMEŞE, Osman(1)
- AYGÜL, İmdat(1)
- Akar, Bulent(1)
- Akşit, Berfin(1)
- Arserim-Uçar, Dılhun Keriman(1)
- Aslı İnan Ergün(1)
- Atalay, Abdurrahman(1)
- Ayna, Adnan(1)
- Ayten Köksal(1)
- BALTACI, Cemalettin(1)
- Bak, Funda Erşen(1)
- Baltaci, Cemalettin(1)
- Baltacı, Cemalettin(1)
- Berktas, Serap(1)
- Budak, Burhan(1)
- Canbolat Gültekin, Deniz(1)
- Cinemre, Sedanur(1)
- Demirel Ozbek, Yagmur(1)
- Duzgun, Azer Ozad(1)
- Erturk, Omer(1)
- FIDAN, Muhammed Said(1)
- Gidik, Betul(1)
- Gültekin, Deniz Canbolat(1)
- Ilyasoglu, Huri(1)
- Karakurt, Aylin(1)
- Kocyigit, Emine(1)
- Koçak, Tevfik(1)
- Mesci Dindar, Esra(1)
- Münevver Darak(1)
- Okumus, Funda(1)
Yayınlar
- MakaleYalnız künye2026Scopus SJRQ3Effects of Dietary Fibers Obtained from Cold-Press By-products on the in vitro Digestion of Spirulina
Berktas, Serap
This study aimed to investigate the effects of dietary fibers obtained from cold-pressing by-products on the bioactivity of Spirulina (SP) during in vitro digestion. For this purpose, insoluble dietary fibers (CCDL and KCDL) were extracted from cold-pressing by-products of flaxseed and black cumin. SP was combined with CCDL and KCDL, and subjected to an in vitro digestion protocol (INFOGEST). The results showed that the total phenolic content of SP decreased after oral and gastric digestion with the addition of dietary fiber. After small intestinal digestion, the total phenolic content increased. Antioxidant activity values (DPPH and ABTS) decreased in the presence of dietary fiber, while chlorophyll values increased after small intestinal digestion. In particular, in the presence of CCDL, the chlorophyll content increased from 1.61 mg g(-1) to 4.48 mg 2 g(-1) after small intestinal digestion. Furthermore, the bioactivity values of SP after in vitro digestion varied depending on the type of dietary fiber. While KCDL was effective in increasing phenolic compound content, CCDL was more effective in increasing chlorophyll content. Considering the bioactivity values of SP after oral, gastric, and small intestinal digestion, it was determined that the values increased especially in the small intestine. This highlights the possibility that the bioaccessibility and therefore the bioavailability, of SP can be increased through the incorporation of dietary fiber. The addition of bioactive compounds to the development of functional foods rich in dietary fiber has advantages for the food industry in terms of designing and improving healthy foods. This study emphasizes that the in vitro digestion of Spirulina is regulated in the presence of CCDL and KCDL, and that the addition of dietary fibers may contribute to the potential health benefits of Spirulina.
- MakaleAçık Erişim2026Scopus SJRQ3Impacts of Phenolic Compounds Alone and in Combination with Natural Substances or Drugs on Cancer Cells
Budak, Burhan; Sarikaya, Sevim Beyza Ozturk
Phenolic compounds (PCs) are secondary metabolites found in the root and flower parts of plants that help protect the plant from pathogens. The amount and content of the plant species and the environment in which they live vary. Because PCs have antioxidant properties, they have a preventive effect against oxidising substances. Therefore, PCs in the human diet contribute to the prevention of cancer, osteoporosis, diabetes, and many other diseases. Cancer is the most dangerous and deadly disease among these diseases today. Although many chemotherapeutic drugs are used against cancer, the tendency to use other drugs or other applications has increased because the effectiveness of chemotherapeutic drugs is low and healthy cells are damaged in the process. PCs boost the effectiveness of chemotherapeutic drugs against cancer cells while, preserving the health of other cells. In this review, in vivo and in vitro studies conducted in cancer lines, applications of PCs alone, combined applications of PCs with natural substances, and the effects of PCs with drugs were investigated and discussed. In conclusion, gallic acid, ellagic acid, caffeic acid, and ferulic acid are frequently used in cancer treatment. These findings highlight the potential of PCs as versatile adjuvants in cancer treatment, which can increase drug efficacy, protect healthy cells, and minimise toxicity. Consequently, they highlight the therapeutic potential of PCs as complementary agents in cancer treatment strategies.
AtıfOpenAlex1
İncele - MakaleAçık Erişim2026Scopus SJRQ1Effect of sloe berry (Prunus spinosa L.) powder addition on physicochemical, rheological, antioxidant, and sensory properties of stirred yoghurt: a multivariate approach
Yeniçeri, Esra; Ürkek, Bayram; Şengül, Mustafa
In this study, stirred yogurt was produced using sloe berry powder at different ratios (0, 2, 4, 6, and 8%) and stored for 21 days. The effect of sloe berry powder addition on physicochemical properties was limited, whereas the effect of storage period was more pronounced. Lactic cocci and lactic bacilli counts ranged between 7.78–8.93 log CFU/g and 6.19–9.13 log CFU/g, respectively. Yeast and mold were detected in all samples after 14 days of storage. All color parameters were significantly affected by sloe berry powder addition (p <.001), whereas storage period had no significant effect (p >.05). Total phenolic content and antioxidant capacity increased with increasing levels of sloe berry powder. Only the potassium (K) content was affected by the addition of sloe berry powder. Viscosity and consistency values increased in yogurts containing 6% and 8% sloe berry powder on days 14 and 21 of storage. Sensory scores decreased in all samples at the end of storage. Principal component and correlation analyses indicated that the addition of sloe berry powder and storage period exerted distinct effects on the evaluated parameters. Overall, sloe berry powder can be considered a promising ingredient for the development of nutritional and functional stirred yogurt.
- MakaleYalnız künye2026Scopus SJRQ2Phenolic Profiling of Laurus nobilis by LC–ESI–MS/MS: In Vitro Bioactivities and In Silico Enzyme Docking
Bak, Funda Erşen; Canbolat Gültekin, Deniz; Akmeşe, Osman; Atalay, Abdurrahman; Turan, İbrahim; Gültekin, Ergün; Demir, Selim; Aliyazicioglu, Yüksel
This study evaluated the branch, leaf, and fruit extracts of Laurus nobilis collected from Artvin in terms of phenolic composition, antioxidant, antimicrobial, and antidiabetic activities and additionally investigated the binding of selected phenolics to α-amylase/α-glucosidase targets through molecular docking. Using LC–ESI–MS/MS analysis, 31 phenolic compounds were quantitatively identified, with epicatechin, luteolin-7-O-glucoside, and rutin predominating particularly in the branches and leaves. Antioxidant capacity was higher in branches and leaves (total phenolic content [TPC]/FRAP/DPPH IC50: branches and leaves, respectively, 95.27–60.84 mg gallic acid equivalent [GAE]/g, 206.16–162.05 mg Trolox equivalent [TE]/g, and 46.95–113.49 µg/mL). Antimicrobial activity was observed within a minimum inhibitory concentration (MIC) range of 1.25–10 mg/mL; leaves exhibited the strongest effect against Bacillus cereus (1.25 mg/mL), whereas fruits were most effective against Candida albicans (1.25 mg/mL). In antidiabetic evaluation, fruits yielded the lowest IC50 values against α-amylase and α-glucosidase (4.31 and 8.97 mg/mL, respectively), suggesting a “composition-over-quantity” effect despite low total phenolic content. Docking results supported high binding affinities of rutin (α-glucosidase −9.3 kcal/mol; α-amylase −8.1 kcal/mol) and luteolin (−8.6 kcal/mol for both enzymes). Overall, although branches and leaves exhibited strong antioxidant profiles, fruits showed more pronounced inhibitory effects on digestive enzymes; therefore, fractionation studies and in vivo/kinetic validations are recommended.
AtıfScopus3WoS3OpenAlex6
İncele - MakaleAçık Erişim2026Scopus SJRQ3Chemical Profiling of Stachys cretica subsp. anatolica Rech. f. (Endemic) Essential Oils and their Methanol Extracts with Evaluation as Enzyme Inhibitors, Antioxidant, and Antimicrobial Agents
Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman; Aygül, İmdat
Enzyme inhibition activities, phenolic compounds, antioxidant activities, bioactive compounds, antimicrobial activities, and chemical components of essential oil and methanol extracts obtained from the aerial parts of S. cretica subsp. anatolica were investigated. The main phenolic compounds of aerial parts were catechin, oleuropein, and epicatechin. The determined enzyme inhibitor activities highlight the potential of S. cretica subsp. anatolica as a source of bioactive compounds, particularly for carbonic anhydrase and cholinesterase inhibition. The essential oil and methanol extract exhibited remarkable activities against CA-II, AChE, and BChE, although they were less potent than standard inhibitors. The essential oils generally showed stronger antimicrobial activity compared to the 30% methanol extracts across most bacterial and fungal strains, as evidenced by minimum lethal concentration (MLC) and lower minimum inhibitory concentration (MIC) values and larger inhibition zones. Chloramphenicol used alone exhibited the highest antimicrobial efficacy, with the lowest MIC and MLC values and the largest inhibition zones. The essential oils of S. cretica subsp. anatolica were determined as esters, oxygenated sesquiterpenes, and aldehydes in aerial parts. The main components were found to be hexahydrofarnesyl acetone in the aerial parts.
AtıfScopus3İncele - MakaleAçık Erişim2026Scopus SJRQ4Assessment of the effect of conventional and contemporary drying methods on the phenolic profile modulation and antioxidant retention of Trabzon persimmon (Diospyros kaki L.)
Koçak, Tevfik; Demirel Ozbek, Yagmur; Saral, Ozlem; Kocyigit, Emine
This study investigated the effects of conventional and modern drying methods on the total phenolic content, antioxidant activity, and phenolic compound composition of Trabzon persimmons (Diospyros kaki L.) fruits harvested from the same tree and at comparable maturity stages were subjected to sun drying, shade drying, oven drying, air-fryer drying, and microwave drying techniques, with raw samples serving as the control group. The total phenolic content was assessed via the Folin–Ciocalteu method, and the antioxidant capacity was evaluated using the ferric reducing antioxidant power (FRAP) and DPPH radical scavenging activity assays. The identification and quantification of phenolic compounds were conducted using the HPLC technique. The results indicated that the drying process produced statistically significant variations in the phenolic content and antioxidant capacity of Trabzon persimmons. The microwave drying technique resulted in highest total phenolic content and antioxidant activity, whereas both sun and shade drying procedures showed higher values than raw samples. The total phenolic content and antioxidant capacity of samples dried in an air fryer were found to be comparable to those of raw samples. The profile of phenolic compounds varied according to the drying process employed; certain compounds exhibited an increase with specific methods, while others were identified solely under particular conditions. Thus, it has been shown that drying techniques can have a significant impact on preserving and improving the functional properties of persimmons. Furthermore, we believe that microwave drying could be an effective alternative, particularly in terms of preserving bioactive compounds.
AtıfScopus1OpenAlex1
İncele - MakaleYayıncıda açık erişim2026Scopus SJRQ1Chitosan-Based Colloidal Delivery Systems as Natural Carriers for Propolis: Physicochemical Characterization and Application in Walnut Sausages
Mesci Dindar, Esra; Arserim-Uçar, Dılhun Keriman; Ayna, Adnan
In this study, a chitosan-based colloidal delivery systems (CDs) was developed as a natural delivery system for phenolic compounds from propolis extracts obtained via accelerated solvent extraction (ASE) and evaluated for improving the functional properties of walnut sausages. ASE propolis extract was incorporated into a chitosan (C)-based colloidal delivery system formulated with varying concentrations of diacetyl tartaric acid ester of mono- and diglycerides (DATEM) and lecithin (L). The formulation containing 2.5% chitosan, 12% DATEM and propolis, with a particle size of 265.9 nm and a ζ-potential of +40.8 mV, was selected for further characterization and food applications. The phenolic encapsulation of CDs depended on the structural characteristics of individual phenolic compounds, with chrysin (70.51%), vanillin (65.12%), and benzoic acid (51.26%) exhibiting the highest encapsulation; CD-treated walnut sausages decreased the lipid oxidation and improved the storage stability of the walnut sausages during storage. These findings indicate that the chitosan-based colloidal delivery system is a promising carrier for improving the encapsulation of propolis phenolics in walnut sausages.
- MakaleYalnız künye2026Scopus SJRQ2LC–MS/MS Phenolic Profiling and Antioxidant, Antimicrobial, and Antidiabetic Activities of Caucasian Propolis
Gültekin, Deniz Canbolat; Turan, İbrahim; Akmeşe, Osman; Gültekin, Ergün; Demir, Selim; Aliyazicioglu, Yüksel
Propolis is a natural bee product rich in phenolic and flavonoid compounds and is well known for its remarkable biological properties. In this study, the phenolic compositions of propolis samples collected from various localities in the Caucasus region of Türkiye were characterized using LC–MS/MS, and their antioxidant, antidiabetic, and antibacterial activities were also evaluated. LC–MS/MS analyses identified caffeic acid, ferulic acid, and p-coumaric acid as the major phenolic acid compounds, while pinocembrin, chrysin, and galangin were determined as the major flavonoid constituents. Overall, these findings indicate that the antioxidant capacity of propolis samples obtained from different regions may be particularly associated with these key phenolic and flavonoid compounds. Consistent with this composition, total phenolic and flavonoid contents ranged from 34.72 to 120.54 mg GAE/g and 6.88 to 47.19 mg QE/g, respectively. The extracts exhibited a relatively high antioxidant capacity, with FRAP values between 60.47 and 311.87 mg TE/g and DPPH IC50 values ranging from 215.80 to 37.96 µg/mL. Regarding antidiabetic activity, the IC50 values for α-amylase and α-glucosidase inhibition were determined as 5.86–3.47 and 12.22–5.02 mg/mL, respectively, indicating a pronounced antidiabetic potential. Antimicrobial analyses revealed activity against E. faecalis, B. cereus, E. coli, K. pneumoniae, and C. albicans. Overall, this study provides a comprehensive LC–MS/MS-based phenolic profile of Caucasian propolis and demonstrates its antioxidant properties together with its antidiabetic potential.
AtıfScopus2WoS2OpenAlex4İncele - MakaleAçık Erişim2026Scopus SJRQ3Chemical Profiling of Essential Oils and Evaluation of Enzyme Inhibition, Antioxidant, and Antimicrobial Activities in Both Methanol Extract and Essential Oils of Centaurea oltensis
Aygül, İmdat; Akşit, Berfin
The phytochemical composition and biological activities of Centaurea oltensis, an endemic species in Türkiye, were studied. GC–MS/FID analysis identified 128 compounds representing 99.2% of the EO, with decanoic acid (15.1%), α-pinene (7.9%), and sabinyl acetate (5.4%) as the principal constituents. GC–MS/FID analysis identified 128 compounds representing 99.2% of the essential oil (EO), with decanoic acid (15.1%), α-pinene (7.9%), and sabinyl acetate (5.4%) as the principal constituents. LC–MS/MS analysis of the methanol extract (ME) revealed high concentrations of ferulic acid (2410 µg/g dw) and chlorogenic acid (1170 µg/g dw), together with caffeic, salicylic, and 4-hydroxybenzoic acids. The ME exhibited potent carbonic anhydrase-II inhibition (IC₅₀ = 0.028 µg/mL) and significant α-glucosidase inhibition (IC₅₀ = 0.255 mg/mL). The EO showed strong acetylcholinesterase and butyrylcholinesterase inhibitory activities, with IC₅₀ values of 0.0145 µg/mL and 0.204 µg/mL, respectively. Antioxidant assays, including DPPH, ABTS, and FRAP, demonstrated superior activity of the ME, correlating with its higher phenolic and flavonoid contents. The EO displayed remarkable antibacterial activity against Staphylococcus aureus (MIC = 0.02 mg/mL) and moderate antifungal activity against Candida albicans. Overall, C. oltensis demonstrated considerable chemical diversity and promising antioxidant, antimicrobial, neuroprotective, and enzyme inhibitory potential for future pharmacological and therapeutic applications.
- MakaleAçık Erişim2025Scopus SJRQ2Enzyme inhibitory, antioxidant, antimicrobial activities, and phenolic profiles of the methanol extract of Gelasia sericea an endemic species
AYGÜL, İmdat; AKMEŞE, Osman; SEFALI, Abdurrahman; BALTACI, Cemalettin; ÖZ, Mehmet; FIDAN, Muhammed Said
This study aimed to investigate, for the first time, the phytochemical composition, antioxidant, antimicrobial, and enzyme inhibitory properties of the methanolic extract of Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N.Kilian. The extract demonstrated a moderate yield (6.55 g/100 g dry plant material) and was found to be rich in phenolic acids, particularly ferulic acid (17182.97 μg g-1), followed by caffeic acid, protocatechuic acid, and p-coumaric acid. Antioxidant analyses revealed high total phenolic (388.86 mg GA eq./100 g), total flavonoid (74.03 mg QE eq./100 g), and total antioxidant capacity (503.33 mg AA eq./100 g). The extract exhibited remarkable ABTS radical scavenging activity (95.99%, IC50 = 37.59 mg ml-1) and considerable ferric-ion reducing power (974.78 mg FeSO4 eq./100 g). Antimicrobial assays indicated moderate inhibitory effects, with stronger activity against Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis) and the yeast Candida albicans, whereas Gram-negative bacteria were less susceptible. The extract’s MIC values ranged between 0.20-3.25 mg ml-1, and MLC values between 0.41-6.50 mg ml-1, which were higher than those of standard antibiotics. Enzyme inhibition assays revealed moderate activity against carbonic anhydrase II (IC50 = 0.0136 µg ml-1) and weak inhibition of cholinesterases and α-glucosidase, while α-amylase inhibition was relatively more pronounced (IC50 = 13350.00 µg ml-1). Overall, these findings highlight G. sericea as a valuable natural source of phenolic acids and antioxidants, with promising potential for applications in functional foods, nutraceuticals, and phytopharmaceuticals. However, the relatively limited antimicrobial and enzyme inhibitory activities suggest the need for further studies on bioavailability, in vivo efficacy, and compound isolation to optimize its practical applications.
- MakaleAçık Erişim2023Scopus SJRQ3Chemical Composition of Pistacia terebinthus L. and its Phytochemical and Biological Properties
Fidan, Muhammed Said; Baltaci, Cemalettin; Öz, Mehmet; Akar, Zeynep
Vitamin C, enzyme activities, phenolic compounds, antioxidant capacity, antimicrobial activity, and essential oil analyses of ripe and unripe fruits of P. terebinthus were investigated. Vitamin C amounts of ripe and unripe fruits were 63.2 and 15.4 mg/100g, respectively. The main phenolic compounds of unripe and ripe fruits are rutin, syringic acid, and gallic acid. It was determined that the enzyme inhibitor activities in the ripe and unripe fruits were 0.136 mg/mL and 2.14 mg/mL. In all of the free radical scavenging (DPPH and ABTS) activity, ferric (III) ion reducing antioxidant power (FRAP) capacity, total phenolic substance amounts (TPC), total flavonoid substance amounts (TFC), and total antioxidant activity (TAC) antioxidant methods analyzed with plant parts, the methanol extracts obtained from the ripe fruits of the P. terebinthus showed higher antioxidant properties than the methanol extracts obtained from the unripe fruits. Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 showed antimicrobial activity against microorganisms, while methanol extracts obtained from unripe fruit samples did not show antimicrobial activity against the microorganisms used. The chemical grade with the most compounds in the essential oils of P. terebinthus were monoterpenoids, sesquiterpenes, and monoterpenes in unripe and ripe fruits, respectively. The main components were a-pinene with 22.8% and 27.3% ratios in unripe and ripe fruits, respectively.
AtıfWoS9OpenAlex11İncele - MakaleYalnız künye2023Scopus SJRQ2LC-HRMS Profiling and Phenolic Content, Cholinesterase, and Antioxidant Activities of Terminalia citrina
Özler, Ebubekir; Topal, Fevzi; Topal, Meryem; Öztürk Sarıkaya, Sevim Beyza
Terminalia citrina (T. citrina) belongs to the Combretaceae family and is included in the class of medicinal plants in tropical countries such as Bangladesh, Myanmar, and India. The antioxidant activities of lyophilized water (WTE) and alcohol extracts (ETE) of T. citrina fruits, their phenolic content by LC-HRMS, and their effects on cholinesterases (ChEs; AChE, acetylcholinesterase, and BChE, butyrylcholinesterase) were investigated. Especially ten different analytical methods were applied to determine the antioxidant capacity. Compared with similar studies for natural products in the literature, it was determined that both WTE and ETE exhibited strong antioxidant capacity. Syringe and ellagic acids were higher than other acids in ETE and WTE. IC50 values for ETE and WTE in DPPH radical and ABTS⋅+ scavenging activities were calculated as 1.69–1.68 μg mL−1 and 6.79–5.78 μg mL−1, respectively. The results of the biological investigations showed that ETE and WTE had an inhibition effect against ChEs, with IC50 values of 94.87 and 130.90 mg mL−1 for AChE and 262.55 and 279.70 mg mL−1 for BChE, respectively. These findings indicate that with the prominence of herbal treatments, T. citrina plant may guide the literature in treating Alzheimer's Disease, preventing oxidative damage, and mitochondrial dysfunction.
AtıfScopus13WoS8OpenAlex14İncele - Yüksek Lisans TeziAçık Erişim2022Geleneksel tıpta kullanılan kuşburnu (Rosa canina L.) meyvelerinin farklı saklama koşulları ve demleme sürelerinde antioksidan antimikrobiyal aktivitelerinin ve fenolik içeriğinin incelenmesi
Münevver Darak
Rosa canina L (kuşburnu) geleneksel tedavide yaygın kullanılan tıbbi aromatik bir bitkidir. Antioksidan, antimikrobiyal ve anti-inflamatuar özellikleri çeşitli çalışmalarla ortaya konmuştur. Fenolik bileşiklerin çeşitli saklama sıcaklığı ve süresine bağlı olarak miktarlarında değişiklik meydana geldiği bilinmektedir. Bu çalışmada Gümüşhane İli'nden toplanmış kuşburnu meyvelerin farklı demleme süreleri ve saklama koşullarına göre antioksidan, antimikrobiyal aktivite ve C vit miktarı değişimlerinin izlenmesi amaçlandı. Buna göre taze, kurutulmuş ve dondurulmuş kuşburnu örnekleri üç ay ara ile 90 derece sıcaklıkta demlenerek 30, 60, 90 ve 120 dakika 5 ve 6. saatlerde alınan örneklerde ABTS, DPPH FRAP metotları ile antioksidan aktivite, toplam fenolik içerik ve toplam flavonoid madde miktarları ve antimikrobiyal aktivite analizleri, HPLC ile fenolik içerik ve C vitamini analizi yapılmıştır. Kurutulmuş meyvelere ait sulu ve etanolik ekstraklar yapılarak bu örneklerde de yukarıda belirtilen analizler gerçekleştirilmiştir. Demleme ürünlerinde en yüksek antioksidan aktivitenin toplam fenolik içerik ve flavonoid madde miktarının kurutulmuş kuşburnu örneklerinde özellikle 6. Saatte olduğu görüldü (p<0.05). Saklama süresine bağlı olarak bu parametrelerde belirgin azalma izlendi. Demlemeler içinde en yüksek C vitamini miktarı kurutulmuş kuşburnunda olduğu görüldü (83.56 µg/mL demleme). HPLC fenolik içerik analizinde ekstraktlarda toplam 8 adet fenolik bileşik tespit edildi. En fazla Fenolik bileşik olarak kateşin (661.42 µg/g ekstrakt) bulundu. Etanolik ve sulu ekstrakt C vitamini değerlerinde 3 ay sonunda sırası ile %26 ve %14'lük düşüşler tespit edildi. Antimikrobiyal aktivite analizinde 7 suştan beşine karşı (Bacillus subtilis, Staphylus aureus, Streptococcus pyogenes E. Coli, Pseudomonas aeruginosa) aktivite gözlendi. Bu sonuçlara göre kuşburnu meyvelerinin uygun demleme süre ve saklama biçimlerinin verimin arttırılmasına katkı yapacağı düşünülmektedir.
- Yüksek Lisans TeziAçık Erişim2022Puerarin bileşiğinin in vitro şartlarda antioksidan kapasitesinin ve bazı metabolik enzimler üzerine aktivitesinin belirlenmesi
Aslı İnan Ergün
Tez çalışmasında fenolik mokekül yapısına sahip puerarin bileşiğinin asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) enzimleri üzerine inhibisyon etkileri incelendi. AChE enzimi için elde edilen IC50 değeri 177.73 nM iken BChE için 315.07 nM olarak hesaplandı. Standart molekül olarak takrin kullanıldı. Takrin molekülünden daha etkili inhibisyon gösterdiği belirlendi. Tezin diğer kısmında ise aynı molekül için antioksidan aktivitesini değerlendirildi. Bu amaçla, Fe3+-Fe2+ indirgeme kapasitesi tayini, Azino-bis (3-etilbenzo-tiyazolin-6-sülfonik asit) (ABTS) radikali giderme aktivitesi, Cu2+-Cu+ indirgeme kapasitesi (CUPRAC metodu), 2,2- 1,1-Difenil 2-pikril hidrazil (DPPH) serbest radikalleri giderme aktivitesi, DMPD radikali giderme aktivitesi yöntemleri kullanıldı. Referans antioksidanlar olarak BHT, BHA, askorbik asit (C vitamini), troloks ve α-tokoferol (E vitamini) kullanılarak karşılaştırmaları yapıldı. ABTS radikal giderme aktivitesi için Puerarin molekülünün 20 mg/mL konsantrasyonunda % inhibisyon miktarı %99.33 olarak hesaplandı. İndirgeme kapasitelerininde standartlarla paralel olduğu belirlendi. Elde edilen sonuçların kozmetik, ilaç ve biyokimyasal yeni çalışmalara ışık tutacağı düşünülmektedir.
- MakaleYalnız künye2022EFFECT OF REPLACEMENT OF WHEAT FLOUR WITH BLACK LENTIL FLOUR ON BREAD QUALITY
Çelik, Meryem; Ilyasoglu, Huri
The aim of this study was to investigate the effect of black lentil flour (BLF) on the quality of wheat bread. The control bread with wheat flour and BLF-supplemented breads (2, 6 and 10 %, respectively) were prepared and analyzed in terms of proximate composition, physicochemical and sensorial properties, total phenolic content (TPC), and antioxidant activity. The BLF-supplemented breads provided significantly higher protein compared to the control bread (p < 0.05). Moreover, it improved significantly TPC and antioxidant capacity (p < 0.05). However, the addition of BLF reduced significantly the specific volume of the bread, L* and b* values of both crumb and crust (p < 0.05). The BLF had no influence overall acceptability. Our findings revealed that the BLF may a have potential use to enhance the quality of wheat bread.
AtıfWoS1İncele - Yüksek Lisans TeziAçık Erişim2021Öğrekotu (Heracleum platytaenium boiss.) ekstraktlarının antioksidan kapasitesinin belirlenmesi
Ayten Köksal
Bitkiler, bitkilerden elde edilen ürünler ve saflaştırılmış maddeler birçok çalışmaya konu olmakta ve olmaya devam etmektedir. Bitkilerin öncelikle kendilerini korumak için ürettikleri sekonder metabolitleri olan antioksidanlar gerek gıdayla gerek saflaştırılmış halleriyle tüketildiklerinde insanlığa birçok yönden fayda sağlamaktadırlar. Bu nedenlerle Apiaceae (Maydanozgiller) ailesinden olan öğrek otu (Heracleum platytaenium Boiss.) bitkisinin yaprak ve çiçek kısımlarının su ve etil alkol ekstraktlarının antioksidan kapasitesi araştırılmıştır. Antioksidan aktivitesini değerlendirmek amacıyla; 1,1-Difenil 2-pikril hidrazil (DPPH) serbest radikalleri giderme aktivitesi, 2,2-Azino-bis (3-etilbenzo-tiyazolin-6-sülfonik asit) (ABTS) radikali giderme aktivitesi, Cu2+-Cu+ indirgeme kapasitesi (CUPRAC metodu), Fe3+-Fe2+ indirgeme kapasitesi tayini, total fenolik bileşik ve total flavonoid miktar tayinleri yapılmış olup, BHA, BHT, α-Tokoferol, ve Troloks referans antioksidan olarak kullanılarak karşılaştırma yapılmıştır. Çalışma kapsamında indirgeme kapasitesi metotlarında sadece CUPRAC metodunda Troloks standardına yakın değerler gözlemlendi. Fe3+-Fe2+ indirgeme kapasitesi yönteminde ise ekstraktların hepsinde standartlara göre düşük radikal giderme aktivitesi gözlemlendi. DPPH serbest radikalleri giderme aktivitesine bakıldığında sadece yaprak-su (63 μg mL-1) ekstraktının BHT (63 μg mL-1) gibi davrandığı, diğer ekstraktların yaprak-alkol (86.63 μg mL-1), çiçek-alkol (99.00 μg mL-1), çiçek-su (115.5 μg mL-1) ise düşük kapasiteye sahip olduğu belirlendi. ABTS radikallerini giderme kapasitesine ait IC50 değerleri; çiçek-su (3.73 μg mL-1) > yaprak-su (3.69 μg mL-1) > α-Tokoferol (3.32 μg mL-1) > çiçek-alkol (3.12 μg mL-1) > Troloks (2.90 μg mL-1) > BHA (2.83 μg mL-1) > yaprak-alkol (2.72 μg mL-1) > BHT (2.60 μg mL-1) sıralamasında aktivite göstermiştir. Yüksek IC50 değeri düşük antioksidan aktivite anlamına geldiğinden yaprak alkol ekstraktının bu yöntemde BHA, Troloks ve α-Tokoferol standartlarına göre daha iyi aktivite gösterdiği tespit edilmiştir.
- MakaleAçık Erişim2021Scopus SJRQ1Chemical compositions by using LC-MS/MS and GC-MS and antioxidant activities of methanolic extracts from leaf and flower parts of Scabiosa columbaria subsp. columbaria var. columbaria L.
Akar, Zeynep
The members of the Scabiosa genus are one of the traditional medicinal plants used in the treatment of many diseases, in particular the treatment of scabies. In this study, it was aimed to determine antioxidant activities and chemical composition of methanolic extracts of leaves and flowers of Scabiosa columbaria subsp. columbaria var. columbaria. The phenolic contents of both parts of the plant were analyzed by LC-MS/MS. A total of 6 phenolic compounds were determined and chlorogenic acid was the major compound in both flower and leaf parts of the plants, with 5936.052 mg/g and 8021.666 mg/g, respectively. 6 different methods were used to determine the antioxidant activity of the plant parts. Both leaf and flower parts of the plant showed high antioxidant activity in all tested methods and the antioxidant activity values of the leaf part were measured higher than those of the flower part for four tests. The methanol extracts of the plant parts was analyzed with GC-MS and number of the essential oil compounds in the leaf and flower parts were determined as 17 and 13, respectively. Linalool compound was also found to be common in both parts of the plant. The major compounds of the essential oils were identified as 4Octadecenal (30.01%) in the flower and carvone (35.44%) in the leaf. In addition, terpene derivatives was determined as 90.32% of the highest essential oil group in the leaf, while this value was determined as 1.42% in the flower. For the flower, aromatics were determined as the main component group with 21.31%. (c) 2021 The Author. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
AtıfWoS22OpenAlex29İncele - MakaleAçık Erişim2020RP-HPLC-UV Analysis of the Phenolic Compounds, Antimicrobial Activity Against Multi-Drug Resistant Bacteria and Antioxidant Activity of Fruit and Seed of Diospyros lotus L.
Akar, Zeynep; Karakurt, Aylin; Okumus, Funda; Cinemre, Sedanur; Duzgun, Azer Ozad; Akar, Bulent; Can, Zehra
The object of the present study was to investigate the antioxidant, antimicrobial activity and phenolic compounds of fruit and seeds of Diospyros lotus L. The fruits consumed by humans as nutrients were harvested from the plants that grow naturally in the province of Trabzon-Akçaabat, Eastern Black Sea Region of Turkey. The antioxidant activities of fruit and seeds of Diospyros lotus were determined by using four methods (% DPPH radical scavenging activity, FRAP antioxidant power determination, CUPRAC reducing antioxidant activity and total phenolic content (TPC) in five different proportion of methanol-water extracts. All extracts of fruit and seeds of D. lotus analyzed through whole antioxidant analysis methods showed significant antioxidant activity. In addition, antimicrobial activity of fruit and seeds extracted with DMSO was determined against seven standard bacteria and three multi-drug resistance clinical strains. Although fruit extracts did not have the antimicrobial activity against bacteria, seeds showed antimicrobial effect to both standard strains (Gram-negative and Gram-positive) and antibiotic resistance clinical isolates (Klebsiella pneumoniae carrying blaKPC, Acinetobacter baumannii, harboring blaOXA-23 gene and resistance to colistin, and multi-drug resistant Pseudomonas aeruginosa). MICs’ value of plant seed extracts for standard strains was 0.75-25 mg/mL and antibiotic resistant clinical bacteria were 12.5 and 25 mg/mL. Additionally, phenolic compounds in methanol extracts of fruit and seeds were also determined in by HPLC using 19 standards. Gallic acid and chrysin phenolic compounds were the major phenolic compounds in fruit and seeds, respectively.
AtıfScopus8OpenAlex9TR Dizin3İncele - MakaleYalnız künye2019Scopus SJRQ4DETERMINATION OF ANTIOXIDANT, ANTIMICROBIAL ACTIVITIES, PHENOLIC COMPOUNDS OF WILD ROSA L. SPECIES BAYBURT, TURKEY
Gidik, Betul; Akar, Zeynep; Can, Zehra; Sefali, Abdurrahman; Erturk, Omer
Five different rosehip (Rosa L.) species including, Rosa canina, Rosa iberica Stev, Rosa villosa, Rosa beggeriana Schrenk and same kind of but different location Rosa spinosissima grown in Bayburt from Turkey are studied. The wild species of Rosa L. were collected from Rocks and Steps, Field and garden borders. Morphological parameters were measured as fruit and seed weights and fruit and seed size. Ten fruits were chosen randomly for the measurement and take the average of them to determine the weights and size. In our study was determined antioxidant activity, antimicrobial activity and phenolic compounds. As antioxidant activity; total phenolic total flavonoid, condense tannin contents, CUPRAC, FRAP, ABTS. Nineteen standard of phenolic compositions of fruits and seeds were determined. Antioxidant, antimicrobial activities of the extracts were found high levels in rose hip species fruit than seed. Catechin, rutin protocatequic acid and CAPE were principal for all rose hip species fruit but, was not deteceted CAPE Kop Mountain, Yukari Kop Village Rosa spinosissima species. This suggests that the difference in location affects the phenolic content. Pearson Correlation analyze has done with the morphological parameters, GPS data, antioxidant activity and phenolic compounds by using the SPSS software programme version 25.0.
AtıfWoS3İncele