Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2021Investigation of class 1 integrons with antibiotic resistance genes in multidrug-resistant acinetobacter baumannii strains and determination of plant extract effects on multidrug-resistant isolates
Saral, Ayşegül; Cinemre, Sedanur; Okumuş, Funda; Akar, Zeynep; Çiçek, Ayşegül Çopur; Düzgün, Azer Özad
This study aimed to investigate the presence of resistance genes in multidrug-resistant A. baumannii isolates as well as to determine the antibacterial activity of selected plant extracts against isolates. 41 strains were isolated from various clinical samples. PCR tests were performed using the primers. Methanol was used as solvent for the preparation of the plant extracts. MIC values of the plant extracts were determined by the broth microdilution method. The blaOXA-23, blaCTX-M-1, blaCTX-M-2, blaGES genes and Class 1 integrons were detected in five isolated strains. The lowest MIC value (2.25 mg/mL) was determined for the Echinacea purpurea extract, while the highest MIC value (50 mg/mL) was determined for the Morus alba extract. Determination of the antibacterial effect of plants extracts used in the study against A. baumannii isolates shows the importance of screening the antibacterial activity of plants in the fight against antibiotic resistance.
- MakaleAçık Erişim2020RP-HPLC-UV Analysis of the Phenolic Compounds, Antimicrobial Activity Against Multi-Drug Resistant Bacteria and Antioxidant Activity of Fruit and Seed of Diospyros lotus L.
Akar, Zeynep; Karakurt, Aylin; Okumus, Funda; Cinemre, Sedanur; Duzgun, Azer Ozad; Akar, Bulent; Can, Zehra
The object of the present study was to investigate the antioxidant, antimicrobial activity and phenolic compounds of fruit and seeds of Diospyros lotus L. The fruits consumed by humans as nutrients were harvested from the plants that grow naturally in the province of Trabzon-Akçaabat, Eastern Black Sea Region of Turkey. The antioxidant activities of fruit and seeds of Diospyros lotus were determined by using four methods (% DPPH radical scavenging activity, FRAP antioxidant power determination, CUPRAC reducing antioxidant activity and total phenolic content (TPC) in five different proportion of methanol-water extracts. All extracts of fruit and seeds of D. lotus analyzed through whole antioxidant analysis methods showed significant antioxidant activity. In addition, antimicrobial activity of fruit and seeds extracted with DMSO was determined against seven standard bacteria and three multi-drug resistance clinical strains. Although fruit extracts did not have the antimicrobial activity against bacteria, seeds showed antimicrobial effect to both standard strains (Gram-negative and Gram-positive) and antibiotic resistance clinical isolates (Klebsiella pneumoniae carrying blaKPC, Acinetobacter baumannii, harboring blaOXA-23 gene and resistance to colistin, and multi-drug resistant Pseudomonas aeruginosa). MICs’ value of plant seed extracts for standard strains was 0.75-25 mg/mL and antibiotic resistant clinical bacteria were 12.5 and 25 mg/mL. Additionally, phenolic compounds in methanol extracts of fruit and seeds were also determined in by HPLC using 19 standards. Gallic acid and chrysin phenolic compounds were the major phenolic compounds in fruit and seeds, respectively.
- MakaleAçık Erişim2019Determination of antibiotic resistance genes and virulence factors in Escherichia coli isolated from Turkish patients with urinary tract infection
Özad Düzgün, Azer; Okumuş, Funda; Saral, Ayşegül; Çopur Çiçek, Ayşegül; Cinemre, Sedanur
Introduction: Escherichia coli ranks among the most common sources of urinary tract infections (UTI). Methods: Between November 2015 and August 2016, 90 isolates of E. coli were isolated from patients at Rize Education and Research Hospital in Turkey. Antibiotic susceptibility was determined for all isolates using the Kirby-Bauer disk diffusion method. These E. coli isolates were also screened for virulence genes, β-lactamase coding genes, quinolone resistance genes, and class 1 integrons by PCR. Results: With respect to the antibiotic resistance profile, imipenem and meropenem were effective against 98% and 90% of isolates, respectively. A high percentage of the isolates showed resistance against β lactam/β lactamase inhibitor combinations, quinolones, and cephalosporins. PCR results revealed that 63% (57/90) of the strains carried class 1 integrons. In addition, a high predominance of extended-spectrum β-lactamases (ESBLs) was observed. The qnrA, qnrB, and qnrS genes were found in 24 (26.6%), 6 (6.6%), and 3 (3.3%), isolates, respectively. The most common virulence gene was fim (82.2%).The afa, hly, and cnf1 genes were detected in 16.6%, 16.6%, and 3.3% of isolates, respectively. Moreover, we observed eleven different virulence patterns in the 90 E. coli isolates. The most prevalent pattern was fim, while hly-fim, afa-aer-cnf-fim, aer-cnf, afa-aer, and afacnf- fim patterns were less common. Conclusions: Most of the E. coli virulence genes investigated in this study were observed in E. coli isolates from UTI patients. Virulence genes are very important for the establishment and maintenance of infection.