Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Acet, Tuba(7)
- Özcan, Kadriye(5)
- Çorbacı, Cengiz(2)
- Akar, Bülent(1)
- Akay, Seref(1)
- Baltacı, Cemalettin(1)
- Birinci, Emrah(1)
- Bozkir, Buğra(1)
- Burnaz, Nesibe Arslan(1)
- Capar, Tugba Dursun(1)
- Dedebas, Tugba(1)
- Düzgün, Azer Özad(1)
- Ekici, Lutfiye(1)
- Erdoğdu, Esma Gülbahar(1)
- Karakullukçu, Volkan(1)
- Karatas, Seyda Merve(1)
- Karpuz, Omer(1)
- Kavuncuoglu, Hatice(1)
- Kotiloğlu, Deniz(1)
- Küçük, Murat(1)
- Ozcan, Kadriye(1)
- Sancak, Kemal(1)
- Sefalı, Abdurrahman(1)
- Soylu, Serkan(1)
- Yalcin, Hasan(1)
- Yiğittürk, Gürkan(1)
- Çelik, Fatih(1)
- Özdilek, Hasret(1)
- Ünver, Yasemin(1)
Yayınlar
- MakaleAçık Erişim2025Scopus SJRQ4DYE REMOVAL PERFORMANCE AND BIOLOGICAL ACTIVITIES OF IRON OXIDE NANOPARTICLES SYNTHESIZED FROM Cotoneaster sp. FRUIT EXTRACT
Akar, Bülent; Baltacı, Cemalettin; Düzgün, Azer Özad; Karpuz, Omer; Karakullukçu, Volkan; Erdoğdu, Esma Gülbahar
This work aimed to study the antioxidant, antibacterial, and dye adsorption capabilities of the aqueous extract of Cotoneaster sp. fruits (CFE) and iron oxide nanoparticles (CF-FeONP) green-synthesized from the CFE. CF-FeONPs were characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) analyses. The antioxidant activity of CFE and CF-FeONPs was assessed through various tests, including ABTS radical scavenging activity, DPPH radical scavenging activity, iron-reducing antioxidant power (FRAP), total phenolic content (TPC), total flavonoid content (TFC), and total antioxidant content (TAC). The CF-FeONPs had superior antibacterial and antioxidant activity compared to the CFEs. Furthermore, CF-FeONPs suppressed the growth of Gram-negative bacteria such as Yersinia pseudotuberculosis ATCC 911, Escherichia coli ATCC 25922, Proteus vulgaris ATCC 13315, and Pseudomonas aeruginosa ATCC 43288, as well as by Gram-positive bacteria like Streptococcus pyogenes ATCC 19615, Staphylococcus aureus ATCC 25923. and Bacillus subtilis ATCC 6633. The microdilution medium technique was used to determine the minimum inhibitory concentration (MIC) of CFE and CF-FeONPs against these bacteria strains. The adsorption rates of CF-FeONPs exhibited variability based on the quantity of adsorbent used and the specific dye type, whereas the lowest adsorption rate was 250 mg/L. CF-FeONPs demonstrated higher adsorption capacities for malachite green (MG) dye compared to methylene blue (MB) and phenol red (PR).
AtıfScopus2WoS2OpenAlex1İncele - MakaleAçık Erişim2025Scopus SJRQ2The biological activity and phenolic content of endemic Astragalus baibutensis
Ozcan, Kadriye; Acet, Tuba; Yiğittürk, Gürkan
Astragalus baibutensis, is a plant endemic to Turkey, belonging to Fabaceae family, that is used as a folk medicine and food in this country. The purpose of this study is to find out the biological activities such as antioxidant, antimicrobial, enzyme inhibitory and DNA protective activity and also analyse the phenolic contents of the plant. To determine of antioxidant activity ABTS and DPPH assays were performed. Disc diffusion and microdilution methods were used to evaluate the antimicrobial activity. Additionally, enzyme inhibitory activities such as α-amylase, α-glucosidase, and tyrosinase were determined by using spectrophotometric methods. DNA protective activity was detected using pUC19 Plazmid DNA. The antiproliferative effect on SKOV-3 human ovarian cancer cells was examined by WST-8 assay. In addition, the phenolic contents were analysed by using HPLC. The biological activity of ethyl acetate extract of the root was found to be higher than those of other extracts. This extract had the highest amount of apigenin as phenolic compound. On the other hand, extracts showed moderate inhibition against the tested microorganism and antiproliferative properties in a concentration-dependent manner toward ovarian cancer cells. According to the results, Astragalus baibutensis has the potential for pharmaceutical and food additive usage.
AtıfScopus1WoS1OpenAlex1İncele - MakaleAçık Erişim2024Scopus SJRQ2Design and fabrication of low-cost microsystems for production of chitosan particles; [Kitosan parçacıklarının üretimi için düşük maliyetli mikrosistemlerin tasarımı ve fabrikasyonu]
Akay, Seref; Özdilek, Hasret
Recent research has focused on the methods for administration of medications in a controlled manner while minimizing side effects. Cross-linked hydrogels have wide application area as nanocarriers. There are many approaches for developing suitable methods to produce ideal particles. Advances in microtechnology have been considered as a solution to produce controlled drug delivery systems, however, it is limited due to the necessity for specialized laboratories. In this study, a simple method to produce antibiotic-loaded chitosan particles is proposed. Production was carried out with two different designs, a syringe-based flow focusing system (outer diameter of 1 mm, inner diameter of 120 µm) and microchannel with 250 µm width and 100 µm height fabricated with the computer numerical control (CNC). Sodium Triphosphate (TPP) was used as cross-linking agent for the production of particles and the effects of the concentration and flow rates on the particle formation was investigated. It has been observed that particles can be produced in a syringe-based system, but the resulting particles are not homogeneous. In the studies carried out with CNC microchannel, it was determined by SEM analysis that the obtained particles ranged in size from 39 to 771 nm, and it was seen that the CNC microchannel was more effective at low and equal flow rates (25 µl/min). It has been found that increasing the flow rate and concentration of chitosan increases the particle size. It was determined that Ampicillin loaded particles synthesized with the CNC microchannel exhibited high inhibition against Gram positive and Gram negative bacteria. These results show that the proposed approaches for the design of microsystems can be used in the formation of drug carriers without the need for specialized laboratories and expertise.
AtıfScopus1İncele - MakaleYalnız künye2023Scopus SJRQ2Phenolic Components, Mineral Composition, Physicochemical, and Bioactive Properties of Opuntia ficus-indica with Different Drying Methods
Capar, Tugba Dursun; Dedebas, Tugba; Kavuncuoglu, Hatice; Karatas, Seyda Merve; Ekici, Lutfiye; Yalcin, Hasan
Cactus pear fruit (Opuntia ficus-indica) have nutritional and antioxidant properties due to the presence of vitamins, fibers, minerals, and polyphenols. The present paper aims to examine the effects of drying methods (sun, oven, vacuum, and freeze) on the bioactivity and antimicrobial quality of prickly pear fruit. Total phenolic content (TPC), antioxidant (DPPH) and antimicrobial activity, mineral content, phenolic compounds, and color values were investigated. The highest antioxidant activity (DPPH) and color values were observed in the freeze-dried samples, which were very close to the values for fresh samples. The total phenolic content (TPC) of the freeze-dried sample was 503.53 mg GAE/100 g. The most abundant phenolic compound was p-hydroxybenzoic acid (210 ppm) identified in fresh and freeze-dried fruit extract, which may be more effective antimicrobials than the rest. The mineral content of freeze- and vacuum-dried samples was very close to that of fresh fruit. This study indicates that freeze-drying of prickly pear fruit improved the bioactive and antimicrobial properties without leading to nutritional loss.
AtıfScopus5WoS4OpenAlex3İncele - MakaleYalnız künye2022Scopus SJRQ2Chemical profile, antioxidant, antimicrobial, and enzyme inhibition activities of Pilosella hoppeana subsp. cilicica
Acet, Tuba
Pilosella hoppeana subsp. cilicica has been traditionally used for healing many diseases in folk medicine in Anatolia. However, there are, to date, no studies aimed to verify this local usage with scientific data. In the present study, phenolic content and biological activities such as the antioxidant, antimicrobial and enzyme inhibition activities of different extracts of this plant’s aerial part were investigated for the first time. According to the obtained data, the biological activity of methanol extract was found to be significantly higher than the others. Moreover, the major components detected were chlorogenic acid (61.79 mg g−1 extract), apigenin (2.727 mg g−1 extract), and luteolin (1.110 mg g−1 extract) in this extract. This extract showed a strong enzyme inhibitory activity including α-amylase (96.66 mmol ACAE g−1 extract), α-glucosidase (17.27 mmol ACAE g−1 extract), AChE (10.42 mg GALE g−1 extract), and BChE (102.35 mg GALE g−1 extract). On the other hand, the extract exhibited remarkable antimicrobial effect on Yersinia pseudotuberculosis, and Staphylococcus epidermidis (64 µg/mL MIC value); intermediate (128–256 µg/mL MIC value) antimicrobial activity on Proteus vulgaris, Listeria innocua, and vancomycin-resistant Enterococcus faecium. To sum up, it is clearly seen that Pilosella hoppeana subsp. cilicica has significant potential as a source of bioactive compounds in pharmaceutical industries.
AtıfScopus3WoS3OpenAlex5İncele - MakaleAçık Erişim2022Scopus SJRQ2Investigation of the effects of different extraction methods on some biological activities of Dactylorhiza romana subsp. georgica (Klinge) Soó ex Renz & Taubenheim
Bozkir, Buğra; Acet, Tuba; Özcan, Kadriye
Dactylorhiza romana subsp. georgica is found in Anatolia and is traditionally used by local people both as food and to alleviate various ailments. Although various biological activities of the plant have been partially investigated, these properties have not been comprehensively revealed in the light of scientific data. In this study, it was aimed to elucidate the antioxidant, antimicrobial, enzyme inhibition (alpha amylase, alpha glucosidase, and tyrosinase) and DNA protective activity and phenolic content of the extracts obtained from orchid tubers using different extraction methods. For this, the fresh and dry tubers of the plant were extracted with ethanol at different times in an ultrasonic water bath, shaker and their combinations with each other. ABTS, DPPH, and FRAP methods were used to determine the antioxidant properties of plant tubers. In addition, the phosphomolybdate method was used to determine the total antioxidant capacity. The spectrophotometric method was preferred to reveal the enzyme inhibition activity. The antimicrobial activity of the extracts was investigated by microdilution method. In addition, the phenolic content of the plant was analysed by HPLC. The DNA protective activity of the extracts was determined by using the pUC 19 plasmid. According to the results obtained, it was revealed that each extract exhibited various biological activities. According to the HPLC results, the highest phenolic component in the extracts was determined as p-hydroxybenzoic acid. As a result, it was observed that the biological activities of orchid tubers changed according to different extraction methods. Accordingly, due to the high biological activities of orchid tubers, they have a high potential for use in the food and pharmaceutical industries.
AtıfScopus4WoS3OpenAlex5İncele - MakaleYalnız künye2021Scopus SJRQ2Biological activity of Psephellus appendicigerus as a natural agent
Acet, Tuba; Özcan, Kadriye; Sefalı, Abdurrahman
Psephellus appendicigerus, is a plant endemic to Turkey, belonging to the Asteraceae family, which is traditionally used in folk medicine in Turkey. The aim of this study is to reveal for the first time the biological activities of this plant and evaluate its potential use as a natural product. For this reason, different extracts (ethanol, methanol, ethyl acetate, and hexane) of the plant’s aerial parts were used in determining the antioxidant (2,20-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid and 1,1-diphenyl-2-picrylhydrazyl assays), antimicrobial (disc diffusion and microdilution methods), and enzyme inhibition activity (calorimetric methods) related to diabetes (α-amylase and α-glucosidase). Moreover, the phenolic constituents were analysed by high performance liquid chromatography. Methanol extract of the plant exhibited significant biological activity compared to other extracts. This extract had the highest amount of chlorogenic acid as phenolic compound. In addition, all extracts showed inhibition against at least one test microorganism. According to the results, P. appendicigerus has the potential to be used as a natural antioxidant, antimicrobial and enzyme inhibitor.
AtıfScopus2WoS2OpenAlex3İncele - MakaleAçık Erişim2021Scopus SJRQ2Phenolic contents and biological activities of two endemic plants in Turkey
Acet, Tuba; Çorbacı, Cengiz; Özcan, Kadriye
In the paper, the phenolic compounds and biological activity of Corydalis oppositifolia DC. and Senecio cilicius Boiss., the endemic plants growing in Turkey, were investigated for the first time. The ethanol extract of C. oppositifolia exhibited more potent antimicrobial activity while the ethanol extract of S. cilicius exhibited the highest radical scavenging activity on ABTS (81.4±1.1a mg TE g−1 extract) and DPPH (23.4±0.2a mg TE g−1 extract). In C. oppositifolia, the highest total flavonoid and phenolic acid contents were found to be in the ethanol (94.2±3.5a mg QE g−1 extract) and methanol (320.5±3.3c mg GAE g−1 extract) extracts, respectively. In S. cilicius, the highest total flavonoid and phenolic acid contents were found to be in the methanol (56.9±0.9c mg QE g−1 extract) and ethanol (568.0±3.3a mg GAE g−1 extract) extracts, respectively. The ethanol extract of C. oppositifolia has the most prominent activity against α-amylase, AChE and BChE whereas the methanol extract of this plant displayed the highest inhibitory activity against α-glucosidase. HPLC analysis of the ethanol extracts revealed that the major flavonoids were kaempferol and quercetin whereas the major phenolic acids were protocatechuic acid, gallic acid, gentistic acid and 2,4-dihydroxybenzoic acid. The findings suggested that the plants and their extracts have the potential to be used in the treatment of diseases such as diabetes and Alzheimer's diseases.
AtıfScopus6WoS6OpenAlex7İncele - MakaleYalnız künye2020Scopus SJRQ2Phytochemical profile and biological activity of a therapeutic orchid from Anatolia: Dactylorhiza romana subsp. georgica
Kotiloğlu, Deniz; Acet, Tuba; Özcan, Kadriye
In Anatolia, the tubers of Dactylorhiza romana subsp. georgica orchid are consumed as food and frequently used in folk medicine to alleviate the symptoms of some disorders. However, these properties have not been scientifically studied until the present day. In this research, we aimed to identify phenolic profile of the orchid tubers and determine their several features and functions. Among these, there are the antioxidant and antimicrobial effects and the inhibitory effect against diabetes related enzymes (α-amylase and α-glucosidase). In this sense, total phenolic content (Folin–Cicalteu assay), total antioxidant capacity (Phosphomolybdate method), antioxidant (ABTS and DPPH assays) and enzyme inhibition effects of tuber extracts obtained with different solvents (chloroform, ethyl acetate, methanol, ethanol, or hexane) were determined by using spectrophotometric methods, and the phenolic profile was also analysed by HPLC. In addition, antimicrobial activity of the extracts against 3 g (+) and 3 g (−) bacteria and 2 forms of yeasts was determined by disc diffusion and micro dilution methods. Based on our findings, the total phenolic content and antioxidant properties of ethyl acetate extract were considerably higher than the others. The most abundant among the screened phenolic standards was found to be benzoic acid. The highest α-amylase and α-glucosidase enzyme inhibition was measured in methanol and chloroform extracts, respectively. The highest antibacterial (B. cereus) and anticandidal (C. tropicalis and C. albicans) activities were detected in the chloroform and ethyl acetate extracts, respectively. In short, it was clearly seen that the D. romana subsp. georgica tuber had a great potential for being used in food and drug industries as a source of bioactive compounds.
AtıfScopus16WoS10OpenAlex16İncele - MakaleAçık Erişim2019Scopus SJRQ2Centaurea hypoleuca DC: Phenolic content, antimicrobial, antioxidant and enzyme inhibitory activities
Özcan, Kadriye; Acet, Tuba; Çorbacı, Cengiz
Centaurea hypoleuca is one of the plants have been traditionally used in treatments of many diseases in folk medicine in Turkey. In the present study, ethanol, methanol, and ethyl acetate extracts of the plant's aerial parts were examined and their antioxidant, antimicrobial, and enzyme inhibition activities were determined for the first time. The antioxidant assays were performed in vitro using ABTS and DPPH methods, whereas the antimicrobial efficiency was determined using disc diffusion and microdilution methods. Moreover, the enzyme inhibition activity was analyzed using calorimetric methods targeting the key enzymes used in the treatment of Alzheimer's disease (cholinesterase) and diabetes (glucosidase and amylase). Also, the phenolic contents were analyzed using HPLC. The biological activity of flower ethyl acetate extract was found to be significantly higher than those of other extracts. This extract was more prominent because of its strong antimicrobial activity with the MIC value of 8 µg mL−1 against MRSA. Moreover, the main components of the extract were determined to be catechin and chlorogenic acid. In conclusion, these results indicate that C. hypoleuca can be pharmaceutically used as herbal remedies for its antioxidant, antimicrobial, and enzyme inhibitor effects.
AtıfScopus35WoS31OpenAlex39İncele - MakaleYalnız künye2014Scopus SJRQ1New thiophene-1,2,4-triazole-5(3)-ones: Highly bioactive thiosemicarbazides, structures of Schiff bases and triazole-thiols
Ünver, Yasemin; Sancak, Kemal; Çelik, Fatih; Birinci, Emrah; Küçük, Murat; Soylu, Serkan; Burnaz, Nesibe Arslan
Key compound 2-(4-amino-5-oxo-3-(thiophene-2-ylmethyl)-4,5-dihydro-1,2,4- tiazole-1-yl) acetohydrazide (3) was synthesized by reacting hydrazine hydrate with ethyl-2-(4-amino-5-oxo-3-(thiophene-2-ylmethyl)-4,5-dihydro-1,2,4-tiazole- 1yl)acetate (2), obtained in basic media from 4-amino-5-(thiophene-2-ylmethyl)- 2H-1,2,4-triazole-3(4H)-one (1). Compound 3 was converted to thiosemicarbazide derivatives (4a-d) and Schiff base derivatives 6a-e and 7a-e. The treatment of compound 4 with NaOH gave 4-amino-2-((4-(4-aryl)-5-mercapto-4H-1,2,4-triazole-3- yl)methyl)-5-(thiophene-2-ylmethyl)-2H-1,2,4-triazole-3(4H)-ones (5a-d). All newly compounds, well characterized by elemental analyses, IR, 1H NMR, 13C NMR and mass spectral studies were tested for their antioxidant and antimicrobial activities. Thiosemicarbazide derivatives (4a-d) were highly active in two antioxidant tests with 69.0-88.2% DPPH scavenging and 503-1257 μM TEAC values, while the others showed lower or no activity. The results of the two antioxidant tests correlated well. Moreover, Thiosemicarbazide derivatives (4a-d) also showed antibacterial activity against Staphylococcus aureus, Bacillus cereus, and Mycobacterium smegmatis. Thiosemicarbazide group deserves attention in the synthesis of bioactive compounds.
AtıfScopus109WoS110OpenAlex99İncele