Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2021Phenolic contents and biological activities of two endemic plants in Turkey
Acet, Tuba; Çorbacı, Cengiz; Özcan, Kadriye
In the paper, the phenolic compounds and biological activity of Corydalis oppositifolia DC. and Senecio cilicius Boiss., the endemic plants growing in Turkey, were investigated for the first time. The ethanol extract of C. oppositifolia exhibited more potent antimicrobial activity while the ethanol extract of S. cilicius exhibited the highest radical scavenging activity on ABTS (81.4±1.1a mg TE g−1 extract) and DPPH (23.4±0.2a mg TE g−1 extract). In C. oppositifolia, the highest total flavonoid and phenolic acid contents were found to be in the ethanol (94.2±3.5a mg QE g−1 extract) and methanol (320.5±3.3c mg GAE g−1 extract) extracts, respectively. In S. cilicius, the highest total flavonoid and phenolic acid contents were found to be in the methanol (56.9±0.9c mg QE g−1 extract) and ethanol (568.0±3.3a mg GAE g−1 extract) extracts, respectively. The ethanol extract of C. oppositifolia has the most prominent activity against α-amylase, AChE and BChE whereas the methanol extract of this plant displayed the highest inhibitory activity against α-glucosidase. HPLC analysis of the ethanol extracts revealed that the major flavonoids were kaempferol and quercetin whereas the major phenolic acids were protocatechuic acid, gallic acid, gentistic acid and 2,4-dihydroxybenzoic acid. The findings suggested that the plants and their extracts have the potential to be used in the treatment of diseases such as diabetes and Alzheimer's diseases.
- MakaleAçık Erişim2019Centaurea hypoleuca DC: Phenolic content, antimicrobial, antioxidant and enzyme inhibitory activities
Özcan, Kadriye; Acet, Tuba; Çorbacı, Cengiz
Centaurea hypoleuca is one of the plants have been traditionally used in treatments of many diseases in folk medicine in Turkey. In the present study, ethanol, methanol, and ethyl acetate extracts of the plant's aerial parts were examined and their antioxidant, antimicrobial, and enzyme inhibition activities were determined for the first time. The antioxidant assays were performed in vitro using ABTS and DPPH methods, whereas the antimicrobial efficiency was determined using disc diffusion and microdilution methods. Moreover, the enzyme inhibition activity was analyzed using calorimetric methods targeting the key enzymes used in the treatment of Alzheimer's disease (cholinesterase) and diabetes (glucosidase and amylase). Also, the phenolic contents were analyzed using HPLC. The biological activity of flower ethyl acetate extract was found to be significantly higher than those of other extracts. This extract was more prominent because of its strong antimicrobial activity with the MIC value of 8 µg mL−1 against MRSA. Moreover, the main components of the extract were determined to be catechin and chlorogenic acid. In conclusion, these results indicate that C. hypoleuca can be pharmaceutically used as herbal remedies for its antioxidant, antimicrobial, and enzyme inhibitor effects.