Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Comparative evaluation of bioactive properties of Philadelphus coronarius leaf and flower extracts: Antioxidant and enzyme inhibitory activities
Burnaz, Nesibe Arslan; Zemzemoglu, Tuba Eda Arpa
This study comparatively evaluated the bioactive properties of Philadelphus coronarius leaf and flower extracts, focusing on phenolic composition, antioxidant capacity, and enzyme inhibitory activities. Plant materials were collected, dried, powdered, and extracted using 80% ethanol via ultrasound-assisted extraction. Total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities (ABTS, DPPH, and FRAP assays) were determined, along with α-glucosidase and acetylcholinesterase inhibitory activities. The leaf extract exhibited significantly higher phenolic and flavonoid contents (4082.81 mg GAE/100 g DW and 3444.17 mg CE/100 g DW, respectively) compared to the flower extract (1864.06 mg GAE/100 g DW and 869.17 mg CE/100 g DW). Similarly, the antioxidant capacities (DPPH, ABTS, and FRAP values) of the leaf extract were found to be significantly higher than those of the flower extract. In enzyme inhibition studies, the leaf extract demonstrated stronger α-glucosidase inhibitory activity (IC50: 0.0211 mg/mL) than the flower extract (IC50: 0.0523 mg/mL). In contrast, acetylcholinesterase inhibition was similar for both extracts. Overall, the findings indicate that P. coronarius leaves possess a strong bioactive potential, particularly in terms of phenolic compounds, antioxidant capacity, and α-glucosidase inhibitory activity. Therefore, the leaf part of this species may be considered a promising natural source of antioxidants and functional bioactive compounds.
- MakaleAçık Erişim2025A comprehensive assessment of the antioxidant capacity and sensory properties of wheat-based cereal breads produced in different formulations
Burnaz, Nesibe Arslan; Hendek, Muge; Baltaci, Cemalettin
In this study, it was aimed to investigate the physicochemical, sensory and antioxidant properties of some wheat-based cereal bread types that are offered for sale and frequently consumed in the markets in Turkey. Bread types were produced with the help of a home-type bread-making machine, and 100% white wheat flour bread (WB) was used as a control. Sensory evaluations were determined by using the nine-point hedonic scale method. Total phenolic contents were determined by using Folin-Ciocalteau’s method. Antioxidant capacities were determined by using ABTS, DPPH, and FRAP assays. Besides, all data were evaluated by using principal component analysis (PCA) to discriminate the properties of bread types. Whole-wheat bread showed the highest antioxidant potentials (200.00µM TEAC for ABTS, 147.50µM TEAC for DPPH, 116.94µM TEAC for FRAP) and the highest amount of phenolic content (64.30mg GAE/100gDW), but a low overall acceptability score (6.00/9.00). As a result, the addition of wheat-based cereal flours affected the antioxidant, sensory, and physical properties of the produced breads (p<0.05). Different cereal flours added to the bread formulation positively affected the antioxidant properties of all breads, but only wheat bran and oat flour addition positively affected the overall sensory acceptability of the bread types. Hereby this study, individuals will be made aware of consuming bread types enriched with different cereal flours including important micronutrients instead of WB which is so poor in micronutrients. The study also provides convenience to individuals in the production of these breads in a home-type bread-making machine. This study reveals the various data for functional properties of bread types that are consumed less than WB.
- MakaleAçık Erişim2025Gümüşhane İli Aile Sağlığı Merkezlerine Başvuran Bireylerin Fonksiyonel Besinlere Karşı Tutumları
Burnaz, Nesibe Arslan; Kartal, Fatma Tayhan; Aykut, Memnune Kabakuş
Amaç: Bu çalışmada, aile sağlığı merkezlerine başvuran bireylerin fonksiyonel besin kullanımının, fonksiyonel besinler hakkındaki tutumlarının ve kullanım sıklığının belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Bireylerin demografik özellikleri, beslenme alışkanlıkları, fonksiyonel besinlere yönelik farkındalıkları ve tüketimlerini değerlendiren bir anket yüz yüze görüşme yöntemiyle yapıldı. Fonksiyonel Gıdalara Yönelik Tutum Ölçeği ile bireylerin fonksiyonel besinlere yönelik algısı ölçüldü. Bireylerin beden kütle indeksleri, vücut ağırlığı ve boy uzunluğu kullanılarak hesaplandı. Bulgular: Katılımcıların %39,7’si fonksiyonel besin tanımını daha önce duyduğunu, %82,3’ü de fonksiyonel besin tükettiğini bildirmiştir. Fonksiyonel besinlerin sağlık üzerinde etkili olduğuna inanan katılımcıların, bu besinleri daha faydalı ve gerekli bulmakta ve aynı zamanda bu konuda bilgi edinmeye daha isteklidirler (p<0,05). Ayrıca fonksiyonel besinlere yönelik tutum lisans ve lisansüstü eğitim düzeyine sahip olanlarda ilköğretim ve lise mezunlarına göre anlamlı olarak daha yüksektir (p<0,05). Sarımsak, nar, üzüm çekirdeği, yeşil çay, zencefil, çörekotu ve tam tahıllı ürünler bireylerin çoğu tarafından fonksiyonel besin olarak kabul edilmektedir. Bununla birlikte C vitamini ve D vitamini gibi besin takviyeleri de fonksiyonel besin olmamasına rağmen çoğu birey tarafından fonksiyonel besin olarak tanımlanmaktadır. Sonuç: Bulduğumuz sonuçlar, aile sağlığı merkezine başvuran bireylerin büyük çoğunluğunun fonksiyonel besin tüketim alışkanlığına sahip olduğunu, eğitim düzeyi ve fonksiyonel besinlere ilişkin sağlık yararlarının fonksiyonel besinlere yönelik tutum ve kabullenme üzerinde etkili olduğunu göstermektedir. Ayrıca, bulgular, fonksiyonel besinlerin ve bu besinlerin sağlık üzerindeki yararlarının farkındalığını ve tüketimini teşvik etmek için destekleyici bir ortam oluşturma açısından kamu ve özel sektöre bilgi sağlayabilir.
- MakaleAçık Erişim2023Phylogenetic analysis based on the ITS, matK and rbcL DNA barcodes and comparison of chemical contents of twelve Paeonia taxa in Turkiye
Cetiz, Mehmet Veysi; Turumtay, Emine Akyuz; Burnaz, Nesibe Arslan; Özhatay, Fatma Neriman; Kaya, Erdal; Memon, Abdulrezzak; Turumtay, Halbay
BackgroudTwelve taxa of herbaceous Paeonia species were recorded in Turkiye. All definitions were performed morphologically and/or anatomically and there is no study based on DNA barcode sequences. Three barcode regions were sequenced to determine the phylogenetic relationships of Turkish Paeonia taxa. The chemical comparison of roots was also investigated.Methods and resultsThe taxons were collected between May and June 2021 from nine cities. Leaf materials were used for DNA isolation and ITS, matK and rbcL regions were amplified and sequenced. There was no difference among taxa in terms of rbcL sequences. But the ITS and matK regions distinguished 12 taxa and structured them in two groups. ITS region distinguished P. peregrina, P. arietina, and P. tenuifolia from other taxa, while matK region distinguished P. arietina and P. witmanniana from other taxa. Both barcode sequences actually showed that the registration of P. mascula subsp. arasicola was actually 100% similar to P. arietina. ITS was the most polymorphic region (n = 54) followed by matK (n = 9). These sequences could successfully discriminate Paoenia species from each other and diploid P. tenuifolia. The methanolic root (100 gr) extracts were examined for total phenolic and flavonoid content, and antioxidant activities. Significant variation was found for polyphenolic content, and antioxidant properties (TPC from 204.23 to 2343.89 mg, TFC from 7.73 to 66.16 mg, and FRAP from 523.81 to 4338.62 mg). SC50 values of ABTS and DPPH were ranged from 115.08 to 1115.52 mu g/ml and 73.83 to 963.59 mu g/ml, respectively.ConclusionIt was concluded that 11 of 12 taxa had differences in terms of ITS and matK sequences and these region must be used for the correct identification of Turkish Paeonia.
- MakaleAçık Erişim2020Süt tozu katkısının ekmek kalite özellikleri ve işlevselliği üzerine etkisi
Burnaz, Nesibe Arslan; Hendek, Muge
Fonksiyonel gıdalar temel beslenmenin ötesinde insan sağlığı üzerinde potansiyel bir etkiye sahiptirler. Bununla birlikte hastalık riskini de azaltmaya yardımcı olurlar. Son yıllarda ekmeğin, zenginleştirilerek besin değerinin arttırılmasına yönelik çalışmalar önem kazanmıştır. Bu çalışmada, geleneksel ekmeğe, %2.5, 5, ve 10 olmak üzere farklı oranlarda süttozu eklenerek ekmekte kalite, antioksidan ve duyusal özellikleri açısından meydana gelen değişimlerin incelenmesi amaçlanmıştır. Ekmek makinası ile üretilen ekmek numunelerinde, TPC (toplam fenolik içerik), FRAP (demir (II) indirgeyici antioksidan güç), DPPH (2,2-difenil-1-pikrilhidrazil), ABTS (2,29-azinobis-(3-etilbenzotiyazolin-6-sülfonik asit) ve duyusal analizler yapılmıştır. Sonuç olarak, ekmek yapımında belli oranda süttozu katkısının duyusal özellikleri olumlu yönde etkilediği belirlenmiştir. Toplam fenolik içerik 4.86 µg GAE/100g’dan 35.48 µg/100g’a artarken; DPPH, FRAP and ABTS seviyeleri sırasıyla 35.42, 39.17, 10.48 µM TEAC’dan 97.92, 61.11, 21.70 µM TEAC düzeyine yükselmiştir
- MakaleAçık Erişim2020Can an abts antioxidant test be performed without a spectrophotometer?
Burnaz, Nesibe Arslan; Akar, Zeynep
ABTS• + (2,2-azinobis- (3-ethylbenzothiazoline-6-sulphonic acid)) radical scavenging assay iswidely used to determine the antioxidant activity of natural and synthetic substances. As otherantioxidant activity determination methods, a spectrophotometer device is needed to determinethe numerical value of the color formed in ABTS method. Therefore, the dependence on thedevice is disadvantageous due to especially the high cost and restriction of workspace. Toovercome this disadvantage, a new colorimetric method in the determination of antioxidantactivity for ABTS method was developed with the help of a scanner device and free software(Image J). Mixtures (plant extracts and antioxidant standards) in this new colorimetric methodwere prepared as in the spectrophotometric method and, after the incubation period, each ofthese reagent mixtures was dropped onto the thin layer chromatography (TLC) plate. Then thecolors that appeared on the thin layer chromatography were transferred to the computer withthe help of a scanner and CSC50 values (the color value of the antioxidant concentrationrequired to scavenge 50% of the ABTS radical in the test solution using Image J software)were measured with free software of Image J. The same measurements were performedsimultaneously on the spectrophotometer. The results of both methods were compared. Therewas a strong correlation between the new colorimetric method and the spectrophotometricmethod for ABTS. Thus, the new colorimetric method for the ABTS test has become easilyapplicable since no spectrophotometer device is needed and in all environments.
- MakaleYalnız künye2019A new colorimetric method for CUPRAC assay with using of TLC plate
Akar, Zeynep; Burnaz, Nesibe Arslan
Cupric reducing antioxidant capacity (CUPRAC) method is one of the most widely used antioxidant methods. However, its need for a spectrophotometer, as in many other methods, is an important disadvantage. Cupric reducing antioxidant capacity was determined based on color analysis with Image J program in computer with the new method developed in this study and thus, use of spectrophotometer was eliminated. The new colorimetric CUPRAC assay was applied to various standard antioxidants and plant extracts. The reaction mixtures were dropped on TLC plate. The colors formed on TLC were transferred to the computer by using scanner images, and CTEAC value in μmol/L of each sample was determined with the image J program. The CTEAC values were compared with the TEAC values measured with the spectrometric method. A linear relationship was found between both (C)TEAC values of antioxidant standards (R2 = 0.991) and plant extracts (R2 = 0.994). Thus, the new method developed for the CUPRAC antioxidant assay can be easily performed in any environment without spectrophotometer device, and it is a low-cost method.
- MakaleYalnız künye2017Türkiye’ de marketlerde satılan alkolsüz içeceklerin etanol seviyeleri
Burnaz, Nesibe Arslan; Küçük, Murat; Doğan, Hacer; Alkan Türkuçar, Semra; Dolu, Ömer Faruk; Alevci, Aylin; Aktaş Karaçelık, Ayça; Polat, Damla
Etanol, birçok farklı alanda kullanılan temel kimyasallardan biri olmasına rağmen, diyetle alındığında sarhoş edici ve zararlı etkileri yüzünden tıbbi, sosyal ve dini konular bakımından önemli tartışma konularından biri olmuştur. Gıdalardaki ve içeceklerdeki etanolün varlığı, miktarı ve etkilerinin kontrolü önemli araştırma alanlarındandır. Alkolsüz içeceklerle ilgili düzenlemeler ülkeden ülkeye farklılık gösterir. Türkiye’de Türk Gıda Kodeksi Alkolsüz İçecekler Tebliği’ne (2007/26) göre izin verilen en yüksek etanol miktarı 3,0 g/L’dir. Kodekse göre, hacimce % 0,3’ den daha az etanol içeren içecekler, alkolsüz içecekler olarak tanımlanır. Bu çalışmada; kolalar, portakallı sodalar, %100 meyve suları, meyve nektarları, meyve aromalı sodalar, enerji içecekleri, ayran ve kefirin bilinen markaları alkolsüz içecek test numunesi olarak kullanıldı. Bu amaçla, Türk Standartları Enstitüsü’nün TS 1594 Meyve ve Sebze Ürünleri Etanol Muhtevası Tayininin Titrimetrik Yöntemi bir değişiklik yapılarak kullanıldı. Numunelerdeki etanol Clevenger tipi destilasyon aparatıyla hidrodestilasyonla destillendi, ki bu uygulama yöntemi daha uygulanabilir hale getirmiştir. Numunelerde bulunan en yüksek binde etanol konsantrasyonları sırayla kolalarda 0,14, portakallı kolalarda 0,53, meyve aromalı sodalarda 0,88, enerji içeceklerinde 0,47, meyve sularında 1,46 ve süt ürünlerinde 0,25 olarak bulundu. Bütün numunelerin etanol seviyesi kodekste izin verilen sınırın altında bulundu.
- MakaleAçık Erişim2017Antiproliferative and apoptotic effect of Morus nigra extract on human prostate cancer cells
Turan, Ibrahim; Demir, Selim; Kilinc, Kagan; Burnaz, Nesibe Arslan; Yaman, Serap Ozer; Akbulut, Kubra; Mentese, Ahmet; Aliyazicioglu, Yuksel; Deger, Orhan
Background: Morus nigra L. belongs to the family Moraceae and is frequently used in traditional medicine. Numerous studies have investigated the antiproliferative effects of various extracts of different Morus species, but studies involving the in vitro cytotoxic effect of M. nigra extract are very limited. The purpose of this study was to evaluate the phenolic composition and antioxidant activity of dimethyl sulfoxide extract of M. nigra (DEM) and to investigate, for the first time, the probable cytotoxic effect in human prostate adenocarcinoma (PC-3) cells together with the mechanism involved. Methods: Total polyphenolic contents (TPC), ferric reducing antioxidant power (FRAP) and phenolic compounds of DEM were evaluated using spectrophotometric procedures and HPLC. The cytotoxic effect of DEM on PC-3 cells was revealed using the MTT assay. Mechanisms involved in the cytotoxic effect of DEM on PC-3 cells were then investigated in terms of apoptosis, mitochondrial membrane potential and cell cycle using flow cytometry, while caspase activity was investigated using luminometric analysis. Results: TPC and FRAP values were 20.7 ± 0.3 mg gallic acid equivalents and 48.8 ± 1.6 mg trolox equivalents per g sample, respectively. Ascorbic acid and chlorogenic acid were the major phenolic compounds detected at HPLC analysis. DEM arrested the cell cycle of PC-3 cells at the G1 phase, induced apoptosis via increased caspase activity and reduced mitochondrial membrane potential. Conclusions: Our results indicate that M. nigra may be a novel candidate for the development of new natural product based therapeutic agents against prostate cancer.
- MakaleYalnız künye2015Preliminary assessment of a yoghurt-like product manufactured from hazelnut slurry: Study using response surface methodology
Ilyasoğlu, Huri; Yilmaz, Firat; Burnaz, Nesibe Arslan; Baltaci, Cemalettin
The aim of this study was to evaluate the possibility of using hazelnut slurry in manufacture of yoghurt. A yoghurt-like product was prepared from hazelnut slurry fortified with skimmed milk powder. The effects of the total solids content of the hazelnut slurry (TSCHS, 8-16g100g-1) and the content of milk powder (CMP, 6-9g100g-1) on the proximate composition, physicochemical and sensorial properties, fatty acid composition, total phenolic content (TPC) and antioxidant activity of the product were evaluated using response surface methodology. Both the TSCHS and the CMP had a significant effect on the total solids content, b value, syneresis, palmitic and oleic acid content, and FRAP value. Only the TSCHS showed a significant effect on the protein and fat content, a value, water-holding capacity, TPC and ABTS values. Only the CMP showed a significant effect on the carbohydrate and ash content and the acidity. The characteristics of the product generally appeared to be compatible with those of yoghurt. The product was rich in unsaturated fatty acids. Therefore, using hazelnut slurry in manufacture of yoghurt may be proposed to enhance the health benefits of yoghurt.
- MakaleYalnız künye2015Effect of domestic cooking methods on antioxidant capacity of fresh and frozen kale
Ilyasoʇlu, Huri; Burnaz, Nesibe Arslan
The aim of this study was to evaluate the changes in the antioxidant capacity of fresh and frozen kale during domestic cooking methods (e.g., boiling, microwaving, and steaming). The antioxidant activities of the samples were measured using three in vitro assays (1,1-diphenyl-2-picrylhydrazyl assay, 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid assay, and ferric reducing antioxidant power assay). The steaming treatment was found to be the best cooking method to retain antioxidant compounds, followed by the microwaving and boiling treatments. The frozen uncooked samples exhibited a higher antioxidant capacity than the fresh, uncooked samples, indicating that the freezing process could enhance the antioxidant capacity of the kale.
- MakaleYalnız künye2014New thiophene-1,2,4-triazole-5(3)-ones: Highly bioactive thiosemicarbazides, structures of Schiff bases and triazole-thiols
Ünver, Yasemin; Sancak, Kemal; Çelik, Fatih; Birinci, Emrah; Küçük, Murat; Soylu, Serkan; Burnaz, Nesibe Arslan
Key compound 2-(4-amino-5-oxo-3-(thiophene-2-ylmethyl)-4,5-dihydro-1,2,4- tiazole-1-yl) acetohydrazide (3) was synthesized by reacting hydrazine hydrate with ethyl-2-(4-amino-5-oxo-3-(thiophene-2-ylmethyl)-4,5-dihydro-1,2,4-tiazole- 1yl)acetate (2), obtained in basic media from 4-amino-5-(thiophene-2-ylmethyl)- 2H-1,2,4-triazole-3(4H)-one (1). Compound 3 was converted to thiosemicarbazide derivatives (4a-d) and Schiff base derivatives 6a-e and 7a-e. The treatment of compound 4 with NaOH gave 4-amino-2-((4-(4-aryl)-5-mercapto-4H-1,2,4-triazole-3- yl)methyl)-5-(thiophene-2-ylmethyl)-2H-1,2,4-triazole-3(4H)-ones (5a-d). All newly compounds, well characterized by elemental analyses, IR, 1H NMR, 13C NMR and mass spectral studies were tested for their antioxidant and antimicrobial activities. Thiosemicarbazide derivatives (4a-d) were highly active in two antioxidant tests with 69.0-88.2% DPPH scavenging and 503-1257 μM TEAC values, while the others showed lower or no activity. The results of the two antioxidant tests correlated well. Moreover, Thiosemicarbazide derivatives (4a-d) also showed antibacterial activity against Staphylococcus aureus, Bacillus cereus, and Mycobacterium smegmatis. Thiosemicarbazide group deserves attention in the synthesis of bioactive compounds.