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43 →- MakaleAçık Erişim2026Chemical profiling, biological evaluation, and QSAR-informed metabolite prioritization of Hypericum ekerii: a first report on antioxidant, enzyme inhibitory, and cytotoxic activities
Zengin, Gokhan; Yagi, Sakina; Cetiz, Mehmet Veysi; Uba, Abdullahi Ibrahim; Nikolic, Filip; Gasic, Uros; Balos, Mehmet Maruf; Erharman, Ahmet; Dogan, Nurettin; Koyuncu, Ismail
Hypericum ekerii Y & uuml;ce and Ayta & ccedil;, first described in 2017, remains poorly characterized in terms of its phytochemical composition and biological properties. This study investigated aerial-part extracts of H. ekerii prepared with ethyl acetate, acetone, 70% acetone-water, and water. The hydroacetone extract showed the highest total phenolic and flavonoid contents. LC/MS analysis enabled the annotation of 99 constituents, including phenolic acids, flavonoids, xanthones, anthraquinones, and acylphloroglucinols. The hydroacetone extract exhibited the strongest radical-scavenging and reducing activities, whereas acetone and ethyl acetate extracts showed the highest metal-chelating capacity. In enzyme assays, hydroacetone was the most active extract against acetylcholinesterase, tyrosinase, and hCA II, while ethyl acetate showed greater inhibition of butyrylcholinesterase and hCA I. The aqueous extract was the most active against alpha-glucosidase, and hydroacetone and ethyl acetate showed comparable alpha-amylase inhibition. Cytotoxicity remained limited overall; the lowest IC50 value was observed for the ethyl acetate extract against HCT-116 cells (95.09 & micro;g mL-1), with weak selectivity (SI = 1.2). A QSAR-informed prioritization framework identified myricetin as the leading cross-layer candidate, p-coumaric acid as a target-specific priority metabolite, and ellagic acid, quercetin, and caffeic acid as antioxidant-priority candidates. Molecular docking supported favorable binding profiles for selected metabolites, while molecular dynamics simulations were used to assess the stability of representative complexes. Overall, H. ekerii appears to be a chemically diverse species with notable antioxidant and enzyme-inhibitory potential, providing a focused basis for future compound-level validation.
- MakaleAçık Erişim2026Beyond the fruit: phenolic composition, cytotoxicity, antioxidant, and multi-enzyme inhibitory activity of twig and leaf extracts from four understudied Turkish Rosa species
Salehi, Hajar; Terzic, Milena; Ak, Gunes; Zengin, Gokhan; Swiatek, Lukasz; Salwa, Kinga; Yapici, Ismail; Gulcin, Ilhami; Yildiztugay, Evren; Lucini, Luigi
The phytochemical profile and multi-target bioactivity of four Turkish Rosa species, R. canina, R. hemisphaerica, R. horrida, and R. pulverulenta, were evaluated across vegetative organs (twigs and leaves) and three solvent polarities (ethyl acetate (EA), methanol (MeOH), water), in total 24 extracts. Untargeted Ultra-High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-QTOF-MS) metabolomics, along with total phenolic (TPC) and flavonoid (TFC) content, six antioxidant assays and inhibition of seven enzymes were determined. Twig EA extracts displayed unexpectedly high TPC (83-105 mg gallic acid equivalent (GAE) per g) and TFC (35-47 mg rutin equivalent (RE) per g), indicative of selective aglycone flavonoid enrichment. The phenolic profiles identified 337 putative compounds, of which 60.84% belonged to the flavonoid class. The semi-quantitative analysis also indicates that methanol and water extracts had higher quantities of glycosylated flavonoids and phenolic acids, while EA extracts were richer in lignans, especially R. horrida-leaf-EA (1743.25 & micro;g SE per g DW), and polyphenols. In enzyme inhibition assays, EA extracts demonstrated consistent multi-target activity: AChE inhibition (up to 2.49 mg galantamine equivalent (GALAE) per g), BChE inhibition (up to 4.44 mg GALAE per g), tyrosinase inhibition (up to 63.58 mg kojic acid equivalent (KAE) per g), alpha-amylase inhibition (up to 0.72 mmol acarbose equivalent ACAE per g), and carbonic anhydrase inhibition (CA I IC50 as low as 12.17 & micro;g mL-1). The R. hemisphaerica-twig-EA extract showed highly selective cytotoxic activity against A375 cells, with a 50% cytotoxic concentration (CC50) of 3.22 & micro;g mL-1 and an SI of 73.95, compared to VERO cells. This is the first report of carbonic anhydrase inhibition, BChE-selective activity, cytotoxicity, and comprehensive enzyme-inhibitory data for R. hemisphaerica, R. horrida, and R. pulverulenta. These findings reframe the phytopharmacological potential of Turkish Rosa species beyond their traditionally studied fruit.
- MakaleYalnız künye2026Chemical characterization and bioactivity assessment of Erodium cedrorum subsp. salmoneum extracts supported by in silico analysis
Yagi, Sakina; Zengin, Gokhan; Zoltan, Cziaky; Jeko, Josef; Ivashchanka, Yahor; Turecka, Katarzyna; Halasa, Rafal; Gucwa, Magdalena; Kochan-Jamrozy, Kinga; Stefanowicz-Hajduk, Justyna
The growing interest in natural products, particularly as food additives and pharmaceutical raw materials, is making research into discovering new sources especially important. Erodium cedrorum subsp. salmoneum (family Geraniaceae) is used traditionally to cure many ailments like diabetes and gastro-intestinal diseases. Within this background, the current study was designed to investigate, for the first time, the chemical composition, antioxidant and enzyme inhibitory poroperties of different extracts from the aerial parts and roots of E. cedrorum. Results revealed that the aqueous extract of the aerial parts accumulated the highest total phenolic (85.35 mg GAE/g) and flavonoids (28.65 mg RE/g) contents. A total of 61 and 45 compounds, belonging mainly to phenolic acids and flavonoids, were tentatively identified in the aerial parts and roots extracts respectively. The aqueous extract from the aerial parts exerted the highest antiradical, ions reducing and chelation properties. The EtOH and 70% EtOH extracts of the two organs recorded the best anti-acetylcholinesterase activity (2.18 -2.41 mg GALAE/g; p >= 0.05) while those of the roots recorded respectively the best anti-butyrylcholinesterase and antityrosinase activities. The most potent antibacterial activity was exhibited by the 70% EtOH extracts from aerial parts and the pure EtOH extracts from roots. In contrast, ethyl acetate extracts from both plant parts showed only marginal activity. Among the tested strains, the highest susceptibility was observed against C. sporogenes ATCC 19404, C. bifermentans ATCC 638, S. aureus ATCC 6538, S. aureus ATCC 43300 (MRSA), C. diphtheriae PCM 506, and H. pylori ATCC 43504. The extracts were also examined for their anti-cancer potential on the HeLa, AGS and SKOV-3 cell lines, as well as a non-cancerous cell line. The ethyl acetate extract of the roots exhibited the most potent anti-cancer activity, with the lowest IC50 values. In addition, selected top-ranked complexes against AChE, alpha-amylase, representative bacterial and oncogenic targets were further evaluated by molecular docking and 100 ns molecular dynamics (MD) simulations, indicating overall conformational stability and persistent binding. In conclusion, these results position E. cedrorum as a valuable natural source of antioxidant and enzyme-inhibitory agents, meriting further exploration for drug development purposes.
- MakaleAçık Erişim2026Chemical Constituents and Biological Potentials of Teucrium scordium subsp. scordioides Extracts: In Vitro Experimental and In Silico Perspectives
Akgul, Bengusu Hacer; Yagi, Sakina; Cetiz, Mehmet Veysi; Saka, Enver; Zengin, Gokhan; Llorent-Martínez, Eulogio J.; Gómez-Torres, Marina; Yapici, Ismail; Gulcin, Ilhami; Yildiztugay, Evren
This study was undertaken to examine, for the first time, the antioxidant and enzyme inhibitory properties of several extracts obtained from the aerial parts of Teucrium scordium subsp. scordioides (Schreb.) Arcang (family: Lamiaceae). Among the extracts, the EtOH extract contained the highest total phenolic (89.96 mg GAE/g) and flavonoid (45.74 mg RE/g) contents. Phenylethanoid glycosides were predominant, particularly in the ethanol and aqueous extracts, and teucroside/forsythoside B and verbascoside/forsythoside A were tentatively identified as the major compounds. EtOH and 70% EtOH extracts showed comparable (p > 0.05) anti-DPPH activity (201.89 and 188.52 mg TE/g), Cu++ (562.81 and 547.88 mg TE/g), and Fe+++ (329.13 and 320.08 mg TE/g) reducing capacities. The EtOH extract showed the best antityrosinase activity (72.00 mg KAE/g). The EtOAc extract inhibited human carbonic anhydrase (CA) isoenzymes I and CAII (IC50 = 26.86 and 65.37 mu g/mL, respectively). Complementary in silico analyses of the major phenylethanoid and flavonoid glycosides supported their possible multitarget interaction patterns and identified the Forsythoside A-BChE complex as a computationally favorable model for future experimental validation. Overall, these findings indicate that T. scordium subsp. scordioides may represent a natural source of antioxidants and enzyme inhibitors for use in different health-promoting formulations.
- MakaleAçık Erişim2026The Joy Luck Club’ın Türkçe Çevirisi Yoluyla Göçmen Kültürünün Aktarımı
Fidan, Giray; Bilge, Topaloğlu Ayşe
Günümüzde yoğun bir şekilde yaşanan göç hareketleri bizi melez kültüre sahip insanları anlamaya yöneltmiş, bunun sonucunda kültür odaklı çevirinin önemi artmıştır. Bu çalışmada, göçmenlere ait melez kültürlerin farklı toplumlara aktarımının bir örnek eser aracılığıyla incelenmesi amaçlanmıştır. Bu incelemeyle, göç olgusunun sonuçlarından biri olan melez kültürel unsurların çevirisinde karşılaşılan durumlar ortaya çıkarılmaya çalışılmıştır. Çalışmada, Çinli bir göçmen ailenin kızı olan ve “Çinli-Amerikalı” olarak adlandırılan, melez bir kimliğe sahip olan yazar Amy Tan’in The Joy Luck Club adlı romanı ve Mehmet Doğan tarafından Talih Kuşu adıyla Türkçeye aktarılan çevirisi örnek olarak seçilmiştir. Öncelikle kaynak eser, Peter Newmark’ın kültürel unsurlar sınıflandırmasına göre analiz edilmiştir. Eserin çevirisi ise, Lawrence Venuti’nin “yerlileştirme” ve “yabancılaştırma” olarak adlandırdığı makro çeviri stratejileri ile Eirlys E. Davies’in “koruma”, “ekleme”, “yaratma”, “küreselleştirme”, “yerelleştirme”, “çıkarma” ve “değiştirme” olan mikro çeviri stratejileri doğrultusunda incelenmiş olup, çevirmenin süreç içerisindeki tercihleri değerlendirilmiştir. Çalışmanın sonucunda, çevirmenin Çin kültürüne ait ögelerin çevirisinde çoğunlukla “yabancılaştırma” stratejisini uyguladığı, ancak bazı durumlarda, özellikle melez kültürel unsurları hedef kültüre aktarma güçlüğünden dolayı “yerlileştirme” stratejisine başvurduğu gözlemlenmiştir. Bu bulgular, kültürel kimliklerin çeviri sürecinde nasıl yeniden biçimlendiğini göstermesi açısından önem taşımaktadır. Ayrıca bu çalışma, kültürlerarası iletişimde çevirmenin rolünün yalnızca dilsel aktarım değil, aynı zamanda kültürel arabuluculuk işlevi taşıdığını da vurgulamaktadır.
- MakaleAçık Erişim2026A Comparative Genre Analysis of Turkish Consumer Reviews in Digital Contexts
Demir, Süleyman
This study presents a genre-based comparative analysis of 100 Turkish product reviews collected from Trendyol and 100 book reviews from Kitapyurdu, aiming to uncover the structural, linguistic, and pragmatic features shaping user-generated digital discourse. Drawing on frameworks of move structure analysis and linguistic evaluation, the research investigates how differing communicative purposes, expectations, and platform affordances influence the way users articulate evaluations, express emotions, and offer recommendations. The findings demonstrate that product reviews generally adhere to a concise and function-oriented structure, often comprising an initial evaluation, followed by a reasoning phase that justifies the user’s stance based on personal experience, and concluding with explicit recommendations or appreciation directed toward sellers or future buyers. These reviews are typically characterized by informal and conversational language, spelling and punctuation irregularities, extensive use of intensifiers, as well as extralinguistic aspects such as emojis that visually reinforce affective meanings. Notably, product reviews frequently mention ideas about packaging quality, delivery speed, or the suitability of the product for a specific family member, enhancing their persuasive impact. In contrast, book reviews display an elaborate rhetorical organization. They often begin with detailed descriptions or definitions of the work, reference the author’s broader literary contributions, and provide interpretative evaluations enriched by thematic summaries or selective quotations. The syntactic complexity, careful punctuation, and lexically diverse style of these reviews signal a deeper cognitive and affective engagement with the content. By illustrating how genre conventions and platform contexts jointly shape linguistic and discursive choices, this study underscores the value of genre awareness in fostering critical digital literacy. It also points to pedagogical applications in language teaching and suggests avenues for future research on digital genres across diverse sociocultural settings, media platforms, and interactional modalities.
- MakaleAçık Erişim2026Corpus-Driven Lexical Profiles of Feminine Words in Turkish: Bayan, Kadın and Karı
Demir, Süleyman
This study aims to examine the semantic and pragmatic features of the three female-referential words “bayan,” “kadın,” and “karı” using a corpus-driven approach. Accordingly, colligations, collocations, semantic preferences, and semantic prosodies of the words were analyzed using concordance lines obtained from the Turkish National Corpus (TNC) with an “extended lexical unit model.” To this end, the concordance lines of each word were subjected to multiple readings, and recurring formal and semantic patterns were classified to determine the semantic relationships between words. The findings show that words have different discursive functions and social representations. The word “bayan” is concentrated in institutional, formal, and neutral contexts; in classificatory and labelling areas of use, while not carrying a distinct emotional prosody. “Kadın,” on the other hand, exhibits a multi-layered pattern of meaning in semantic fields such as emotional evaluation, motherhood/family roles, cultural identities, and political discourse, in addition to physical description; it develops positive and negative prosodies depending on the context. The word “karı,” on the other hand, is used in pejorative, slang, and derogatory contexts; it carries a predominantly negative connotation, producing a distinct negative prosody through insulting adjectives, demeaning comparisons, and masculine possessive discourse. The study demonstrates that female-referenced words are shaped at the level of linguistic choice by gender ideologies, value attributions, and discursive positioning. In this respect, the findings provide important clues for broader sociolinguistic research on how representations of women are constructed.
- MakaleYalnız künye2026Honeybee Silk: A Promising Value-Added Biomaterial with Antioxidant, Antimicrobial, Antihyaluronidase, and Microstructural Characteristics
Yildiz, Oktay; Değirmenci, Atiye; Boyraci, Gulsum Merve; Aksoy, Canan
Bee products with their huge potential are becoming more valuable beyond food industry. Honeybee silk is non-utilized biomaterial in any commercial applications and even burned during the processing of beeswax. Although studies concerning insects silks are commonly on spider and silkworm silks, honeybee silk remains unexplored. Scanning electron microscope and energy dispersive X-ray spectroscopy (SEM–EDX) were used to identify chemical composition via elemental mapping of the native form of honeybee silk. Honeybee silk, in the form of thin layer inside the honeycomb cells, was processed through a series of steps to separate it from beeswax. Protein content was evaluated and revealed a composition of approximately 11.69%, indicating its strong biopolymeric origin. Amino acid analysis of honeybee silk performed by RP-HPLC revealed the presence of both polar and nonpolar amino acids, indicating a well-balanced amino acid profile. Antioxidant, antimicrobial, and anti-hyaluronidase properties of processed honeybee silk were presented. The methanolic extracts of honeybee silk were found to have higher total phenolic content (TPC) and antioxidant activity as assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) assays than the aqueous extracts. While no antimicrobial activity was found for aqueous extracts, methanolic honeybee silk extract exhibited antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacilluscereus, Staphylococcus epidermidis, and Candida albicans. The hyaluronidase inhibition values (IC50) were found as 0.0094 and 0.0079 g/mL for aqueous and methanolic honeybee silk extracts, respectively. The aforementioned findings indicate that honeybee silk as a promosing value-added product is worthy of attention from pharmaceutical industries and future studies.
- MakaleYalnız künye2026Utilizing Pollutant Waste Cheese Whey into Low-cost Broth for Bacterial Growth and Protein Production
Alkowlani, Amir Towfiq Hasan; Beldüz, Ali Osman; Çanakçi, Sabriye; Bektaş, Kadriye İnan; Ay, Fulya; Özyurt, Taner; Yıldız, Oktay
Cheese whey (CW), an abundant and nutrient-rich byproduct of cheese production, accounts for approximately 85% of milk volume and is generated globally in quantities reaching 145–200 million tons annually. Despite its biochemical complexity and high organic load, CW remains underutilized in microbial biotechnology. Its pollutant potential is up to 100 times higher than domestic sewage due to extreme biological oxygen demand (BOD), chemical oxygen demand (COD), high nutrient content, and acidity. Untreated discharge causes severe oxygen depletion, damages aquatic ecosystems, degrades soil, and contributes to climate change. This study evaluates CW as a sustainable and cost-effective alternative to synthetic bacterial growth media. Ten bacterial strains were cultured for 17 h in different forms of CW fresh, aged (up to two years), and dried and compared with standard Luria Bertani (LB) medium. Bacterial growth was assessed using optical density at 600 nm (OD₆₀₀), and protein yields were measured in µg/µL. CW supported growth ranged from OD₆₀₀ 2.36–4.46, comparable to 2.53–4.16 in LB, with protein yields between 0.19 and 0.43 µg/µL versus 0.18–0.44 µg/µL in LB. These findings demonstrate that CW can effectively support bacterial proliferation and protein production, offering a novel valorization route for a major agro-industrial pollutant while reducing environmental harm and fermentation costs.
- MakaleYalnız künye2026Phytochemical profiling, biological activities, and molecular docking analysis of Dorycnium pentaphyllum subsp. haussknechtii
Ayna, Adnan; Hakan, Oguzhan; Yapıcı, İsmail; İzol, Ebubekir; Yılmaz, Mustafa Abdullah; Necip, Adem; Yapar, Yakup
BACKGROUND: In the current study, the biological activity fingerprint of Dorycnium pentaphyllum subsp. haussknechtii was investigated by an interdisciplinary approach through analysis of its phytochemical content, antioxidant capacity, enzyme inhibitory potential and molecular docking. RESULTS: The analysis of the phytochemical content by liquid chromatography–tandem mass spectrometry was characterized by the presence of flavan-3-ols, such as epicatechin, epigallocatechin, and catechin, as well as quercetin-derived flavonoids such as isoquercitrin. Analysis of the antioxidant capacity by 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ferric reducing antioxidant power, Fe3+–Fe2+ reducing and cupric reducing antioxidant capacity methods indicated the strong antioxidant capacity of the plant extract. In addition, the plant extract was able to modulate biological enzyme activity effectively, with IC50 values of 111.78 μg mL−1 for acetylcholinesterase, 22.07 μg mL−1 for butyrylcholinesterase, 1.39 μg mL−1 for α-glucosidase, 7.34 μg mL−1 for carbonic anhydrase I, and 17.41 μg mL−1 for carbonic anhydrase II. To have a better understanding of the molecular mechanism for the plant extract's properties, the major phenolic compounds present in the plant extract were investigated for molecular docking with their respective enzymes. As such, the results revealed a strong binding affinity and conformational stability, with strong hydrogen bonding and π–π interactions. CONCLUSION: Generally, the properties revealed by the plant extract indicate that the phenolic content, strong antioxidant properties, and enzyme inhibition activity of D. pentaphyllum subsp. haussknechtii have shown great potential for being a useful natural source for therapeutic lead compounds.
Tam Kayıt
| Alan | Değer | Dil |
|---|---|---|
| dc.date.accessioned | 2026-10-22T08:47:33.318182655Z | |
| dc.date.available | 2026-10-22T08:47:33.318182655Z | |
| dc.identifier.uri | https://rdlopen.rawdatalibrary.net/r/gumushane/10013 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.title | Rektörlük | tr |
| dc.title | Rectorate | en |
| dspace.entity.type | OrgUnit | |
| organization.identifier.code | gumushane-rektorluk | |
| organization.unitType | other | |
| rdl.unit.alias | \brektorluk\b | |
| rdl.unit.alias | \brectorate\b |