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- MakaleYalnız künye2016Relationships Among some Pears Genotypes (Pyrus Communis L.) Based on ISSR and RAPD Analysis; [Bestimmung der genetischen Verwandtschaft bei Birnen mit Hilfe der ISSR und RAPD Methoden]
Kalkışım, Ozgun; Okcu, Melih; Okcu, Zuhal; Karabulut, Besim; Yildirim, Nalan; Agar, Guleray
Thirty one pears genotypes from east blacksea region were evaluated for genetic relationships by using Randomly Amplified Polymorphic DNA (RAPD) and ISSR (Inter-Simple Sequence Repeats) markers from total 70 RAPD and ISSR primer investigated, 22 could amplify clearly and consistently. Cluster analysis of the pears genotypes was performed based on data from polymorphic bands RAPD and ISSR by using Jaccard’s similarity coefficient and the Unweighted pair-group method with arithmetic average (UPGMA) clustering method. The 31 pear genotypes were classified into two major groups. Cluster A was divided into 2 subclusters: Gumushane pears and Trabzon pears. Cluster B consisted of Artvin pears. The similarity matrix values ranged between 0.105 and 0.968.
- MakaleYalnız künye2015Genetic characterization of the Akkaraman, Morkaraman and Karayaka sheep through microsatellite variation
Das, Huseyin; Turan, Ibrahim; Demir, Selim
Genotypes have to be controlled periodically and necessary precautions should be taken in order to ensure that animal biodiversity is not lost. In this study, the genotypes of the Akkaraman, Karayaka and Morkaraman species from among Turkey’s local sheep were examined at 7 different microsatellite loci. The average allele counts are close to each other and were determined as 8,905±0,483. Whereas the PIC values ranged from between 0,507-0,794 at an average of 0,705. The He and Ho values used to calculate genetic diversity were determined on average as 0,734±0,023 and 0,509±0,038 respectively. According to the Fisher-Exact test result, the populations are not in HWE (P<0.001). Whereas the FIS values that show inter- population genetic diversity were determined as 0,353±0,092 (KRY), 0,328±0,095 (MRK) and 0,237±0,059 (AKK) respectively. In addition, Population Assignment and Principal Coordinate Analysis graphs were formed. When the pairwise FST and Nm, NeiuD,SHUA and G Statistics values used to calculate the genetic similarities and differences are examined, it is observed that the closest relations in terms of genetics is among AKK-KRY followed respectively by AKK-MRK and KRY-MRK.
- MakaleAçık Erişim2015Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers
Nemli, Seda; Kianoosh, Tala; Tanyolaç, Muhammed Bahattin
The common bean (Phaseolus vulgaris L.) is an important crop throughout the world and is highly recommended for many developing countries due to its nutritional components. A retrotransposon-based DNA marker system, interprimer binding sites (iPBSs), developed from reverse transcriptase primer binding sites, was used to determine genetic diversity in common bean accessions. The genetic polymorphism and effectiveness of the marker system were evaluated. A total of 180 polymorphic bands were detected using 47 iPBS primers among 67 common bean accessions, with an average of 4 polymorphic fragments per primer. The genetic similarity between accessions was calculated using the software NTSYS-pc and ranged from 0.09 to 0.99. The average polymorphism information content value for the iPBS markers was 0.73. A model-based clustering method classified the common bean accessions into 4 populations using STRUCTURE software. The results indicated that the iPBS marker method can successfully determine the genetic diversity level in common bean accessions.
- MakaleYalnız künye2015Determination of Genetic Diversity Among Wild Grown Apples From Eastern Black Sea Region in Turkey Using ISSR and RAPDs Markers; [Ermittlung der genetischen Vielfalt bei wildwachsenden Apfelsorten mittels ISSR- und RAPD-Markern in der östlichen Schwarzmeerregion der Türkei]
Okcu, Melih; Kalkışım, Özgün; Okcu, Zühal; Karabulut, Besim; Yildirim, Nalan; Agar, Guleray
In this study, we investigated interspecific variations of apple genotypes using ISSR (Inter-Simple Sequence Repeats) and RAPD (Randomly amplified Polymorphic DNA) technique. Total 19 genotypes of wild grown local varieties were collected from Eastern Black Sea region of Turkey and have been studied for the analysis of ISSR and RAPD profile to examine their relationship. From total 53 RAPD and ISSR primer investigated, 28 could amplify clearly and consistently. All bands obtained from ISSR and RAPDs primer were polymorphic. The dendrogram realized from the RAPD and ISSR markers grouped the 19 genotypes into two major clusters. Cluster I comprised ‘Bey Apple’, ‘Fatty Apple’, ‘Golden Delicious’, ‘Yellow Belly Apple’, ‘Bride Apple’, ‘Green Belly Apple’, ‘Summer Apple’, ‘Yomra Apple’, ‘Tavşanbaşı’, ‘Black Belly Apple’, ‘Chest Apple’, ‘Local Marble’, ‘English Apple’, ‘Willow Apple’, ‘Arabian Girl’, ‘Yellow Hıdır’ and cluster II contained ‘Starking Delicious’, ‘Mahsusa’, ‘Amasya Marble’. Genetic diversity data from this study will be helpful in using and exploiting the wild genetic material for breeding purposes as well as for further research.
- MakaleYalnız künye2013Molecular characterization of some Onobrychis species growing in the eastern Anatolia region of Turkey
Okcu, Melih; Sengul, Suleyman; Sunar, Serap; Agar, Guleray
In this study, we investigated interspecific variations in Sections Dendrobrychis, Hymenobrychis, Heliobrychis, Onobrychis of genus Onobrychis by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique and RAPD. Total eight species collected from different regions of Turkey were studied for the analysis of seed storage protein profiles and RAPD profile to examine their relationship. Electrophoretic data were documented by using a gel documentation system and analysed by using Quantity 1 -D analysis software and also the dendrogram was formed in UPGAMA. The sections Dendrobrychis, Heliobrychis, Hymenobrychis, Onobrychis of genus Onobrychis were firstly determined by morphological classification. In addition to RAPD and the seed protein of these species belonging to these sections were clustered together. The formed dendrogram from SDS-PAGE analysis showed that all studied species constituted two clusters. The first one consisted of O. huetiana subs. bornmuelleri, O. huetiana, O. montana, O. stenostachya and O. atropatana var. grandiflora and the second one O. viciifolia, O. hajastana and O. cornuta. The dendrogram realized from the RAPD markers grouped the Onobrychis spp. into three major clusters. Cluster 1 included O. huetiana subs. bornmuelleri, O. viciifolia, O. huetiana, O. atropatana var. grandiflora, O. montana and O. cornuta, Cluster 2 included O. stenostachya and Cluster 3 O. hajastana.