Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Duran, Celal(6)
- Ozdes, Duygu(6)
- Bekircan, Olcay(2)
- Altunay, Nail(1)
- Aylin Karakurt(1)
- Bektas, Hakan(1)
- Bulut, Volkan Numan(1)
- Camoglu, Aslihan Yilmaz(1)
- Demirci, Hamit(1)
- Elik, Adil(1)
- Gundogdu, Ali(1)
- Karakoy, Tolga(1)
- Mentese, Emre(1)
- Nida Tilki(1)
- Seker, Serdal(1)
- Serencam, Huseyin(1)
- Soylak, Mustafa(1)
- Tilki, Nida(1)
- Tufekci, Mehmet(1)
- Çilesiz, Yeter(1)
Yayınlar
- MakaleYalnız künye2024Development of a New and Facile Carrier Element-Free Coprecipitation Procedure for Separation, Preconcentration and Sensitive Determination of Cu(II) and Cd(II) Ions in Water and Vegetable Samples
Duran, Celal; Ozdes, Duygu; Mentese, Emre; Bektas, Hakan
A facile and sensitive method predicated on carrier element free coprecipitation (CEFC) using 2-(2-(2-(4-bromobenzyl)-1H-benzo[d]imidazole-1-yl)acetyl)-N-ethylhydrazine-1-carbothioamide (BIMANEC) is reported for trace determination of Cu(II) and Cd(II) in vegetables and water. BIMANEC was used for the first time for the quantitative determination of these trace heavy metals. Flame atomic absorption spectrometer (FAAS) was used for quantitation. The preconcentration conditions were optimized with respect to pH (8.0), BIMANEC mass (4.0 mg), volume of sample (100 mL), standing time (5 min), and centrifugation time and rate (3 min and 2500 rpm). The influence of potentially interfering ions was scrutinized for the recovery of analyte ions. Under the optimum conditions, the limits of detection (LODs) were 0.51 and 2.28 µg L−1 for Cu(II) and Cd(II), respectively. The relative standard deviations were < 5% for both. The recoveries from the spiked vegetable samples were from 94.9 to 102.8% and from the spiked water samples between 93.2 and 101.6%. Consequently the developed new, facile, rapid, and highly efficient CEFC process was employed for the trace determination of Cu(II) and Cd(II).
- MakaleAçık Erişim2023A green and simplified approach for the quantitative and sensitive analysis of heavy metal ions in sea and stream waters
Duran, Celal; Camoglu, Aslihan Yilmaz; Ozdes, Duygu; Bekircan, Olcay
Elimination of the matrix effect is a major challenge in developing a method for the quantification of heavy metals (HMs) in water samples. In this regard, the current research describes the simultaneous analyses of Cu(II), Cd(II), and Ni(II) ions in water matrices through flame atomic absorption spectrophotometry (FAAS) after preconcentration with carrier element-free co-precipitation (CEFC) technique by the help of an organic co-precipitant, 3-{[5-(4-Chlorobenzyl)-3-(4-chlorophenyl)-1H-1,2,4-triazol-1-yl]-methyl}-4-[2,4-(dichlorobenzylidene)amino]-1H-1,2,4- triazole-5(4H)-thione (CCMBATT). Based on our literature research, CCMBATT was employed for the first time in this study as an organic co-precipitant for the preconcentration of HMs. Factors such as solution pH, concentration of co-precipitant, sample volume, standing time, centrifugation rate, and time were thoroughly examined and optimized to achieve the highest efficiency in terms of HM recovery. The limits of detection (LODs) (with 10 number of tests) of 0.54, 0.34, and 1.95 μg L-1 and the relative standard deviations (RSD %) of 2.1, 3.3, and 3.0 were determined for Cu(II), Cd(II) and Ni(II) ions, respectively. Recovery results of HMs for the spiked samples were in the range of 92.8-101.0%, demonstrating the trueness of the method and its applicability to the water samples matrix.
- Yüksek Lisans TeziAçık Erişim2022Absisik asitin kadmiyum toksisitesine maruz bırakılan Arabidopsis thaliana sos5 mutantı üzerindeki etkilerinin araştırılması
Aylin Karakurt
SOS5 lokusu (FLA4), bitkilerin tuz stresi koşullarında hücre yüzeyi yapışma proteinini kodlar ve normal hücre genişlemesi, hücre çeperi bütünlüğü, yapısını devam ettirmede gereklidir. Bu genin mutasyonuyla oluşan sos5 (fla4) mutantı, tuz stresi altında, anormal kök genişlemesiyle kısa ve şişman kök fenotipi sergilemektedir. ABA'nın bu fenotipi baskıladığı bilinmektedir. Çalışmamızda, Arabidopsis thalina mutant bitki Cd stresi altında benzer bir kök fenotipi sergilemiştir. Bu durumun Absisik asit (ABA) hormonuyla ilişkili olarak, fizyolojik, biyokimyasal ve moleküler düzeydeki etkileri bilinmemektedir. Bu amaçla, çalışmada 50 µM Cd stresi altında dıştan 5 µM ABA muamelesi ile mutant sos5 bitkisinin büyüme ve gelişmesi, içsel Cd miktarları, lipid peroksidasyonu, prolin, hidrojen peroksit (H 2 O 2 ) miktarları, süperoksit dismutaz (SOD), katalaz (CAT) ve askorbat peroksidaz (APX) enzimlerinin aktiviteleri belirlendi. Stres uygulamasının ardından, RNA izolasyonu yapıldı ve cDNA'lar sentezlendi. ABA1, CYP707A3, RD29A ve RD29B genleri ters transkriptaz enzimi ile analiz edildi. Elde edilen bulgulara göre, Cd stresi altındaki sos5 mutant bitkilere dıştan ABA uygulamasıyla yalnız Cd stresi altındakilere göre, mevcut hücresel hasar ortadan kalkmış, içsel H 2 O 2 değeri azalmış, prolin miktarı artmış ve antioksidan enzim aktiviteleri indüklenmiştir. Bu değişimlere bağlı olarak, mutant bitki fenotipik olarak yabani tipe oldukça benzerlik göstermiştir. Cd stresi altında mutant bitkilerdeki ABA ilişkili gen ifadelerinin yabani tipe göre düşük olduğu belirlenmiştir. Cd stresi altında dışarıdan ABA uygulamasının bitkilerdeki içsel Cd miktarını, sadece stres uygulanan gruplara kıyasla, önemli ölçüde düşürdüğü görülmüştür. Çalışmamızda SOS5 gen lokusunun sadece tuz stresiyle ilişkili olmadığını, aynı zamanda Cd stresi koşullarında da hücre duvarı bütünlüğünü sağladığını göstermesi bakımından önemlidir. Elde edilen bulguların, literatürde önemli katkılar sağlayacağı düşünülmektedir.
- MakaleAçık Erişim2022Insights into brewed tea waste as a green and low-priced adsorbent for solid-phase extraction of Cd(II) ions: isotherm, kinetic, and artificial neural network approach
Ozdes, Duygu; Tilki, Nida; Seker, Serdal; Duran, Celal
In the present research, brewed tea waste (BTW) was utilized as a green, low-priced, and abundant adsorbent for separation/preconcentration of Cd(II) ions through solid-phase extraction method from water and foods for the first time. BTW was applied as a natural adsorbent, without using any chelating agent to bind Cd(II) ions or any chemical reagent for its modification. A three-layer artificial neural network model using backpropagation algorithm was utilized to explicate a prediction model for the extraction performance of Cd(II) ions by selecting the input parameters as solution pH, quantity of BTW, sample volume, eluent concentration and volume, and equilibrium time for desorption. The preconcentration factor, relative standard deviation, and detection limit were attained as 100, 3.03%, and 0.56 µg L−1, respectively. It was decided that the Langmuir isotherm model is acceptable to characterize the retention of Cd(II) ions on BTW. This result pointed out that the active binding sites on the BTW surface are homogeneously distributed. Adsorption capacity of BTW was achieved as 41.5 mg g−1 which is higher than several expensive and difficult-to-prepare adsorbents. Adsorption kinetics was elucidated by pseudo-second order kinetic model. After confirmed the accuracy of the method with spike/recovery studies, it was employed for Cd(II) determination in water (stream and sea water) and food (eggplant, lettuce, parsley, apple, and apricot) samples with high accuracy. The inferences of the study proved that the BTW offers a magnificent application prospect in the extraction of Cd(II) ions.
- Yüksek Lisans TeziAçık Erişim2021Demlenmiş mamul siyah çay atıkları kullanılarak katı faz ekstraksiyonu yöntemiyle çevresel numunelerden Cd(II) iyonlarının ayrılması ve zenginleştirilmesi
Nida Tilki
Bu çalışmada, çeşitli gıda ve su numunelerinden Cd(II) iyonlarının alevli atomik absorpsiyon spektrofotometresiyle (FAAS) tayinlerinden önce katı faz ekstraksiyonu (SPE) yöntemiyle ayrılması ve zenginleştirilmesi amacıyla literatürde ilk defa demlenmiş çay atıklarının (DÇA) adsorban olarak kullanılabilirliği araştırılmıştır. DÇA; FTIR, Boehm titrasyonu ve pHpzc gibi yöntemlerle karakterize edilmiştir. SPE deneyleri kesikli sistemle yürütülmüş olup, Cd(II) iyonlarının desorpsiyonu için 5.0 mL 0.5 M HNO3 çözeltisi kullanılmıştır. Cd(II) iyonlarının kantitatif geri kazanımı için; çözelti pH'ı, DÇA miktarı, eluent türü ve derişimi, adsorpsiyon ve desorpsiyon denge süresi ve numune hacmi gibi temel deneysel değişkenler optimize edilmiştir. Cd(II) iyonları için gözlenebilme sınırı, bağıl standart sapma ve zenginleştirme faktörü sırasıyla 0.56 µg L-1, %3.03 ve 100 olarak hesaplanmıştır. Cd(II) iyonlarının DÇA üzerine adsorpsiyonunda, Langmuir izoterm modelinin uygun olduğu görülmüş olup, bu durum DÇA yüzeyinde bulunan aktif adsorpsiyon bölgelerinin homojen dağılım gösterdiğine işaret etmektedir. DÇA'nın maksimum adsorpsiyon kapasitesi Langmuir izoterm modeli kullanılarak 41.5 mg g-1 olarak hesaplanmıştır. Cd(II) iyonlarının DÇA üzerine adsorpsiyon kinetiğini tanımlamak için ikinci mertebeden kinetik modelin uygun olduğu tespit edilmiştir. Yöntemin doğruluğu ekleme/geri kazanma çalışmaları ile test edildikten sonra yöntem; su numuneleri (dere ve deniz suyu) ve gıda numunelerine (patlıcan, marul, maydanoz, elma ve kayısı) Cd(II) derişimlerinin belirlenmesi için başarıyla uygulanmıştır.
- MakaleYalnız künye2021Preparation of melon peel biochar/CoFe2O4 as a new adsorbent for the separation and preconcentration of Cu(II), Cd(II), and Pb(II) ions by solid-phase extraction in water and vegetable samples
Ozdes, Duygu; Duran, Celal
The present research describes the successful preparation of melon peel biochar modified with CoFe2O4 (MPBC/CoFe2O4) followed by its usage as a new sorbent to separate, preconcentrate, and determine the toxic heavy metal ions by magnetic solid-phase extraction. The metal ion desorption was performed by 0.1 M HCl solution with a volume of 5.0 mL. Flame atomic absorption spectrometry (FAAS) was utilized for detection of the analyte levels. SEM–EDX, TEM, XRD, and FTIR techniques were carried out to illuminate the structure of MPBC/CoFe2O4. The fundamental variables affecting the adsorption and elution efficiencies of the analyte ions including solution pH, MPBC/CoFe2O4 amount, type and concentration of eluent, adsorption and desorption equilibrium time, and sample volume were optimized. The detection limits were calculated as 0.41, 1.82, and 3.16 µg L−1 for Cu2+, Cd2+, and Pb2+ ions, respectively, with the relative standard deviation of lower than 4.2%. There were no substantial interference effects on the analyte ion recovery due to the presence of foreign ions at high levels. Five minutes of contact time was adequate to attain the adsorption equilibrium. The adsorption capacity of MPBC/CoFe2O4 was obtained as 106.4, 65.4, and 188.7 mg g−1 for Cu2+, Cd2+, and Pb2+ ions, respectively, by utilizing Langmuir isotherm model. The pseudo-second order model is favorable to identify the adsorption kinetics. The method was validated by spike/recovery test, and then, it was successfully implemented to determine the aforementioned analyte levels in sea and stream water, pepper, black cabbage, eggplant, and tomato samples.
- MakaleYalnız künye2021Flame atomic absorption spectrometric determination of total chromium and cadmium in bean samples after ultrasonic-assisted microextraction using ionic liquid Aliquat-336
Elik, Adil; Karakoy, Tolga; Çilesiz, Yeter; Altunay, Nail
A new and rapid microextraction approach termed as ultrasonic-assisted ionic liquid-based microextraction (UA-ILME) was developed using ionic liquid Aliquat-336 as extraction solvent for the extraction of chromium and cadmium in bean samples collected from different regions in Turkey. The total amounts of analytes in the samples were measured by flame atomic absorption spectrophotometry. The basis of the extraction step is based on the complexation of Cr(III) and Cd(II) with diethyldithiocarbamate at pH 4.0. The hydrophobic ternary complexes containing analytes were then rapidly extracted from the sample solution to the ionic phase under the ultrasonic effect using tricaprylylmethylammonium chloride (Aliquat-336). Some experimental variables (pH, ligand amount, extraction solvent volume, ultrasound time, extraction temperature and sample volume) were optimized in detail. Following the optimization steps, the limits of detection (LODs, 3Sblank/m) for Cr(III) and Cd(II) were calculated as 0.45 ng mL−1 with linear range of 1.5–380 ng mL−1 and 0.25 ng mL−1 with linear range of 0.8–220 ng mL−1, respectively. Enrichment factors and average recoveries were ranged from 125.8–96.4 to 92.7–106.5%, respectively. The accuracy and precision of the method were validated by analyzing a certified reference material and spiked recovery tests. The UA-ILME method was successfully applied to the determination of Cd and Cr in the samples, highlighting that the matrix ions under consideration do not affect the extraction and determination steps.
- MakaleYalnız künye2016A novel carrier element-free co-precipitation method for separation/preconcentration of lead and cadmium ions from environmental matrices
Bulut, Volkan Numan; Demirci, Hamit; Ozdes, Duygu; Gundogdu, Ali; Bekircan, Olcay; Soylak, Mustafa; Duran, Celal
A new separation and pre-concentration method was presented in this study for two trace heavy metal ions, lead (Pb2+) and cadmium (Cd2+) from aqueous solutions, based on a carrier element free co-precipitation (CEFC) method using a triazole derivate, 3-(4-tert-butylphenyl)−5-phenyl-4-(2-hydroxy-5-methoxybenzylamino)−4H-1,2,4-triazole as an organic co-precipitating agent. The method was optimized with some analytical parameters which affect the quantitative recoveries of study metals. According to the results, optimum pH of solution, quantity of co-precipitating agent, centrifugation and incubation time, and sample volume were determined as 6.0, 1.5 mg, 10 min, and 10 min, and 100 mL, respectively. In addition, no significant matrix interferences were observed over the recovery of the metal ions. For analytical figure of merit, RSD values of the method were found as 5.7 and 4.8 for Pb2+ and Cd2+, respectively. LOD values were also calculated as 2.0 µg L−1 for Pb2+ and 0.2 µg L−1 for Cd2+. The accuracy of method was checked with spiked/recovery tests and analysis of a standard reference material. The developed method was finally applied to the real liquid/solid samples under the optimum conditions.
- MakaleYalnız künye2013Biosorption properties of Morus alba L. for Cd (II) ions removal from aqueous solutions
Serencam, Huseyin; Ozdes, Duygu; Duran, Celal; Tufekci, Mehmet
The abundantly available industrial waste product Morus alba L. pomace (MAP) is one of the cost-effective biosorbent for removal of metal ions from aqueous solutions. Hence, in the present study, we aimed to test the ability of MAP to remove Cd(II) ions through batch biosorption process. Firstly, MAP was characterized using several techniques, and then the influence of various experimental parameters such as initial pH of the aqueous solution, initial Cd(II) concentration, contact time, MAP concentration, and temperature were evaluated upon the biosorption process. It was found that the maximum uptake of Cd(II) ions occurred at initial pH 6.0 and optimum contact time was observed as 60 min. Cd(II) ions adsorption on MAP analyzed by the Langmuir and Freundlich isotherm models and the maximum monolayer biosorption capacity of MAP was found to be 21.69 mg g-1 by using the Langmuir isotherm model. The pseudo-first-order, pseudo-second-order, Elovich, and intraparticle diffusion models were employed to describe the biosorption kinetics. In order to investigate the thermodynamic properties of the biosorption process, the changes in the Gibbs free energy (â̂†G), enthalpy (â̂†H), and entropy (â̂†S) were also evaluated and it has been concluded that the process was feasible, spontaneous, and endothermic in the temperature range of 5-40 C.