Gümüşhane ÜniversitesiGümüşhane ÜniversitesiAçık Erişim Arşivi · RDL Open

Whole-genome characterization of clinical Enterococcus faecalis and Staphylococcus epidermidis Isolates reveals mecA-positive E. faecalis and a shared lnu(A)–rep7a resistance plasmid in Türkiye

MakaleAçık Erişim
  • SDG 3 · Sağlık ve Kaliteli Yaşam

Özet

Background: This study aimed to genomically characterize clinical Enterococcus faecalis and Staphylococcus epidermidis isolates obtained from aspirate and sputum samples at Bayburt State Hospital, focusing on antimicrobial resistance genes, virulence factors, and mobile genetic elements. Methods: Whole-genome sequencing (WGS) was performed on the isolates, and antimicrobial resistance genes, virulence determinants, multilocus sequence types (MLST), plasmid replicons, and mobile genetic elements were identified using bioinformatics tools. Results: The E. faecalis E5 (ST64) isolate, recovered from an aspirate sample, carried multiple antimicrobial resistance genes, including mecA, vanT, erm(B), tet(M), and qacA, along with a diverse virulence gene repertoire (cyl, ebp, ace, and agg), mobile genetic elements (Tn6009, ISL1a3), and four plasmid replicons (rep19C, rep9C, repUS43, and rep7a). The E. faecalis E184 and E187 isolates (ST124), obtained from sputum samples, harbored fewer acquired resistance genes but shared gyrA (T672A) and parC (V307I) mutations associated with fluoroquinolone resistance. The S. epidermidis ST59 isolate carried resistance genes lnu(A), fosB, and qacD, together with the plasmid replicon rep7a and insertion sequence ISSau4. The co-occurrence of lnu(A) and the rep7a plasmid in both species suggests a potential pathway for interspecies transmission of resistance determinants. Conclusions: This study reports the genomic detection of mecA in an E. faecalis isolate from Türkiye based on WGS analysis. However, this finding is based solely on genomic data and lacks phenotypic or experimental validation. The detection of a shared plasmid-associated resistance element (lnu(A)–rep7a) in both E. faecalis and S. epidermidis highlights the potential for interspecies resistance gene flow within the hospital environment.

Anahtar Kelimeler

Antimicrobial resistanceEnterococcus faecalisInterspecies gene transfermecAResistance plasmidStaphylococcus epidermidisWhole-genome sequencing
RDL Kalıcı Bağlantı (PID)
https://rdlopen.rawdatalibrary.net/r/gumushane/13767Resmi atıflarda kullanılan kalıcı adres
DOI
https://doi.org/10.1186/s12866-026-05177-3Yayıncı resmi DOI adresi
Tam Kayıt
AlanDeğerDil
dc.contributor.authorEjder, Nebahat
dc.contributor.authorÖzad Düzgün, Azer
dc.date.accessioned2026-10-22T10:21:45.346778428Z
dc.date.available2026-10-22T10:21:45.346778428Z
dc.date.issued2026
dc.description.abstractBackground: This study aimed to genomically characterize clinical Enterococcus faecalis and Staphylococcus epidermidis isolates obtained from aspirate and sputum samples at Bayburt State Hospital, focusing on antimicrobial resistance genes, virulence factors, and mobile genetic elements. Methods: Whole-genome sequencing (WGS) was performed on the isolates, and antimicrobial resistance genes, virulence determinants, multilocus sequence types (MLST), plasmid replicons, and mobile genetic elements were identified using bioinformatics tools. Results: The E. faecalis E5 (ST64) isolate, recovered from an aspirate sample, carried multiple antimicrobial resistance genes, including mecA, vanT, erm(B), tet(M), and qacA, along with a diverse virulence gene repertoire (cyl, ebp, ace, and agg), mobile genetic elements (Tn6009, ISL1a3), and four plasmid replicons (rep19C, rep9C, repUS43, and rep7a). The E. faecalis E184 and E187 isolates (ST124), obtained from sputum samples, harbored fewer acquired resistance genes but shared gyrA (T672A) and parC (V307I) mutations associated with fluoroquinolone resistance. The S. epidermidis ST59 isolate carried resistance genes lnu(A), fosB, and qacD, together with the plasmid replicon rep7a and insertion sequence ISSau4. The co-occurrence of lnu(A) and the rep7a plasmid in both species suggests a potential pathway for interspecies transmission of resistance determinants. Conclusions: This study reports the genomic detection of mecA in an E. faecalis isolate from Türkiye based on WGS analysis. However, this finding is based solely on genomic data and lacks phenotypic or experimental validation. The detection of a shared plasmid-associated resistance element (lnu(A)–rep7a) in both E. faecalis and S. epidermidis highlights the potential for interspecies resistance gene flow within the hospital environment.
dc.description.sponsorshipGümüşhane Üniversitesi, GU, (A0118.01.01)
dc.description.sponsorshipGümüşhane Üniversitesi, GU
dc.identifier.articlenumber637
dc.identifier.doi10.1186/s12866-026-05177-3
dc.identifier.issn1471-2180
dc.identifier.issue1
dc.identifier.pmid42157088
dc.identifier.scopus2-s2.0-105044815714
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/gumushane/13767
dc.identifier.volume26
dc.identifier.wosWOS:001823939600003
dc.language.isoen
dc.publisherBioMed Central Ltd
dc.relation.ispartofBMC Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntimicrobial resistance
dc.subjectEnterococcus faecalis
dc.subjectInterspecies gene transfer
dc.subjectmecA
dc.subjectResistance plasmid
dc.subjectStaphylococcus epidermidis
dc.subjectWhole-genome sequencing
dc.subject.classificationMicrobiology
dc.subject.otherAnti-Bacterial Agents
dc.subject.otherBacterial Proteins
dc.subject.otherDrug Resistance, Multiple, Bacterial
dc.subject.otherEnterococcus faecalis
dc.subject.otherGenome, Bacterial
dc.subject.otherGram-Positive Bacterial Infections
dc.subject.otherHumans
dc.subject.otherMicrobial Sensitivity Tests
dc.subject.otherMultilocus Sequence Typing
dc.subject.otherPlasmids
dc.subject.otherStaphylococcus epidermidis
dc.subject.otherVirulence Factors
dc.subject.otherWhole Genome Sequencing
dc.subject.otheramikacin
dc.subject.otherchloramphenicol
dc.subject.otherciprofloxacin
dc.subject.othererythromycin
dc.subject.othergentamicin
dc.subject.otherimipenem
dc.subject.otherlevofloxacin
dc.subject.othermacrolide
dc.subject.othermeropenem
dc.subject.othernitrofurantoin
dc.subject.otherquinoline derived antiinfective agent
dc.subject.otherR factor
dc.subject.otherrifampicin
dc.subject.othertetracycline
dc.subject.othertigecycline
dc.subject.othervirulence factor
dc.subject.otherantiinfective agent
dc.subject.otherbacterial protein
dc.subject.otherMecA protein, Staphylococcus epidermidis
dc.subject.otherantibiotic resistance
dc.subject.otherantibiotic sensitivity
dc.subject.otherArticle
dc.subject.otherbacterial gene
dc.subject.otherbacterial strain
dc.subject.otherbacterial virulence
dc.subject.otherbacterium identification
dc.subject.otherbacterium isolate
dc.subject.otherbacterium isolation
dc.subject.otherbiofilm
dc.subject.otherbioinformatics
dc.subject.othercontrolled study
dc.subject.otherdisk diffusion
dc.subject.otherDNA extraction
dc.subject.otherEscherichia coli
dc.subject.othergene flow
dc.subject.othergene insertion sequence
dc.subject.othergene mutation
dc.subject.othergene sequence
dc.subject.othergene transfer
dc.subject.othergenetic variability
dc.subject.otherKlebsiella pneumoniae
dc.subject.othermatrix assisted laser desorption ionization time of flight mass spectrometry
dc.subject.otherminimum inhibitory concentration
dc.subject.othermobile genetic element
dc.subject.othermultidrug resistance
dc.subject.othernonhuman
dc.subject.otherphenotype
dc.subject.otherphylogenetic tree
dc.subject.otherphylogeny
dc.subject.otherplasmid
dc.subject.otherpolymerase chain reaction
dc.subject.otherprevalence
dc.subject.otherreplicon
dc.subject.othersequence analysis
dc.subject.otherbacterial genome
dc.subject.otherdrug effect
dc.subject.othergenetics
dc.subject.otherGram positive infection
dc.subject.otherhuman
dc.subject.otherisolation and purification
dc.subject.othermicrobial sensitivity test
dc.subject.othermicrobiology
dc.titleWhole-genome characterization of clinical Enterococcus faecalis and Staphylococcus epidermidis Isolates reveals mecA-positive E. faecalis and a shared lnu(A)–rep7a resistance plasmid in Türkiye
dc.typeArticle
dspace.entity.typePublication
rdl.citation.openalex0
rdl.citation.scopus0
rdl.citation.wos0
rdl.departmentDepartment of Occupational Health and Safety, Faculty of Health Sciences, Gumushane University, Gümüşhane, Turkey
rdl.oa.statusgold
rdl.oa.urlhttps://doi.org/10.1186/s12866-026-05177-3
rdl.sdgSDG03
rdl.source.indexSCOPUS
rdl.source.indexWOS
rdl.source.indexPUBMED
rdl.wos.indexScience Citation Index Expanded (SCI-EXPANDED)