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- MakaleAçık Erişim2026Scopus SJRQ1Thermochemical Valorization of Silkworm Feeding Waste: Chemical Characterization and Biological Activities of the Pyrolysis-Derived Liquid Fraction
Karan, Halil; Yıldırım, Aybeniz; Yılmaz, Fırat; Özgen, İnanç; Aydoğmuş, Ercan; Gül, Abdulkadir
Silkworm-rearing residues are an underused biomass resource that can be converted into value-added products. In this study, a condensable liquid product (SWP-Liq) was produced from these residues by pyrolysis at 325 ± 5 °C and evaluated for its chemical composition and in vitro biological activities. SWP-Liq yielded phenolic-, flavonoid-, and triterpenoid-equivalent values of 218.47 ± 22.40 mg GAE/g, 79.13 ± 3.55 mg QE/g, and 55.54 ± 6.15 mg EE/g, respectively. The ABTS•+ assay showed a higher radical-scavenging response than DPPH, while Fe3+- and Cu2+-reducing activities were also observed. SWP-Liq inhibited 5-LOX with an IC50 of 33.05 ± 1.10 µg/mL, whereas α-glucosidase inhibition was weaker. Antibacterial activity against all four tested bacterial species was observed at 8 mg/mL but not at 4 mg/mL. Targeted LC–MS/MS quantified five phenolic constituents, with p-coumaric acid (1599.71 µg/g), chlorogenic acid (871.43 µg/g), and ferulic acid (285.50 µg/g) being the most abundant. Overall, these findings support the further investigation of silkworm feeding waste as a feedstock for thermochemical valorization into chemically and biologically evaluable bio-based fractions.
- MakaleYalnız künye2026Scopus SJRQ1Beeswax Waste-Derived Pyrolysis Liquid as a Novel Multifunctional Bioactive Fraction: An Integrated Chemical and Biological Evaluation
Gül, Abdulkadir
In this study, beeswax waste, a low-value by-product of apiculture, was converted into a liquid fraction with bioactive potential through pyrolysis-assisted thermochemical transformation. The LC–MS/MS-based chemical profile, total phenolic content (TPC), total flavonoid content (TFC), antioxidant responses, and antibacterial, antidiabetic, anti-inflammatory, and anticholinesterase activities of beeswax waste pyrolysis liquid (BWPL) were investigated. The TPC and TFC values were determined as 95.30 ± 1.13 mg GAE/L and 51.67 ± 1.12 mg QE/L, respectively. LC–MS/MS analysis revealed benzoic acid and vanillin as the predominant compounds, along with various phenolic acids and flavonoid derivatives. BWPL showed 51.83 ± 1.51% inhibition in the ABTS•⁺ assay and 9.71 ± 0.28% inhibition in the DPPH assay. It also exhibited measurable redox capacity in Fe3+ and Cu2+ reducing power assays. In antibacterial assays, BWPL produced inhibition zones against all tested strains at 1 mg/mL and against E. coli and E. faecalis at 500 µg/mL. The IC50 values of BWPL for 5-LOX and α-glucosidase were 38.17 µg/mL and 1503.0 µg/mL, respectively. In addition, the IC50 values for AChE and BChE were calculated as 112.38 ± 0.87 mg/mL and 13.01 ± 0.42 mg/mL, respectively. PCA and correlation analyses indicated the multidimensional relationships between the LC–MS/MS-based chemical profile and biological activities. Overall, the pyrolytic conversion of beeswax waste into a chemically complex liquid fraction provides a preliminary scientific basis for waste valorization and for further investigation of pyrolysis-derived bioactive fractions.
- MakaleAçık Erişim2026Scopus SJRQ1Valorization of pomegranate peel waste through pyrolysis-liquid-assisted ultrasound extraction: antioxidant, antibacterial and targeted phytochemical characterization
Gül, Abdulkadir; Aydoğmuş, Ercan; Özgen, İnanç; Yılmaz, Fırat; Deniz, Sermin; Arslanoğlu, Hasan
Background: Pomegranate (Punica granatum L.) peel is a phenolic-rich processing by-product, but its valorization commonly relies on conventional solvents. This study examined an integrated approach in which pomegranate peel pyrolysis liquid (PP-PL) was repurposed as the extraction medium for ultrasound-assisted extraction (UAE) of the same biomass. The methodological originality lies specifically in using biomass-derived PP-PL in UAE. Results: PP-PL-assisted extract (PP-E) and an aqueous control extract (AQ-E), prepared from the same peel batch under identical UAE conditions, were compared. The apparent gravimetric recovery of PP-E was 202.5 g kg−1. PP-E showed approximately two-fold higher total phenolic and flavonoid contents than AQ-E. In vitro agar well diffusion screening revealed the largest inhibition zone for PP-E against Staphylococcus aureus at 80 g L−1 (22.65 ± 1.50 mm), whereas AQ-E produced no detectable inhibition zones against the tested strains at the same concentration. PP-E achieved DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS+ (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical-scavenging values of 92.42 ± 0.25% and 97.00 ± 0.17%, respectively, and showed pronounced total antioxidant capacity and iron(III) ion (Fe3+)- and copper(II) ion (Cu2+)-reducing capacities. Targeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) using authentic standards quantified six phenolics, with ellagic acid as the predominant analyte (2015.10 mg kg−1 extract). Conclusion: Integration of pomegranate peel pyrolysis with PP-PL-assisted UAE generated an extract exhibiting in vitro antioxidant properties and antibacterial potential in agar well diffusion screening, providing an alternative route for pomegranate peel valorization. This strategy supports the further development of biomass-derived extraction media for recovering value-added compounds from agro-industrial residues.