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- MakaleYalnız künye2018Scopus SJRQ2The effects of 4-Vinylcyclohexene diepoxide on gene expression in dog testes
Paksoy, Zahid
In this study, the effects of 4-Vinylcyclohexene diepoxide (VCD) on the testicular gene expression in dogs were investigated. Eighteen male dogs were used as material and were randomly allocated to 3 groups. The first group (n=6) was administered sesame oil by intraperitoneal (i.p.) route once a day for 8 days, and this group was served as a control. The second group (n=6) received 80 mg/kg VCD and the third (n=6) group was administered VCD (i.p.) at a dose of 320 mg/kg once daily for 8 days. The testes were removed on following day the last VCD administration. The testes were immediately placed in the RNA stabilization solution and stored at -20°C. Later mRNAs were obtained from tissue samples. After RNA isolation, the cDNAs were synthesized. Finally, MasterMix was prepared and gene expression levels were examined at the real-time PCR. The expression of B cell lymphoma 2 (Bcl-2), tumor suppressor protein (tp53), Bax, CASP2, CASP3, CASP8, CASP9 and β-actin were investigated in the all groups. Cross-group comparisons were made by using student t test. The tp53 gene expression in the second group was found to be higher compared to the control group. It was determined that the expression of Bcl-2 was reduced in the testes of animals treated with high-dose VCD. CASP-3 was found to be more expressed and CASP-9 was down-regulated in the third group. In addition, CASP-8 gene expression was significantly reduced in both treatment groups. As a result, it can be concluded that expression changes in the tp53, Bcl-2, CASP-3, CASP-8 and CASP-9 genes in the testes of male dogs may cause apoptotic changes with the VCD application.
AtıfScopus1İncele - MakaleAçık Erişim2018Scopus SJRQ3The effects of 4-vinylcyclohexene diepoxide on the testes of dogs; [Učinci 4-vinilcikloheksen diepoksida na testise pasa.]
Paksoy, Zahid; Kandemi̇r, Fatih M.; Gokhan, Nezihe; Ozkaraca, Mustafa
This study was aimed at investigating the effects of 4-vinylcyclohexene diepoxide (VCD) on testicular tissue in dogs. For this purpose, 30 mongrel male dogs were used and allocated to 5 groups randomly. The first group (n = 6), used as the control, was administered sesame oil by the intraperitoneal route (i/p). The VCD was administered i/p at doses of 80, 160, 240, 320 mg/kg to the second, third, fourth and the fifth groups (n = 6), respectively, once a day for 8 days. The dogs were surgically castrated on the day after the last VCD injection. The testes were removed immediately and fixed in 10% bouine solution. The testes were subjected to routine tissue processing and examined to determine the testicular damage in each testis. When compared to the control group, it was seen that the seminiferous tubule damage increased significantly in the treatment groups. Malondialdehyde levels reached the highest level in the fifth group, while superoxide dismutase, catalase, glutathione peroxidase activity and glutathione levels were higher in the control group (P<0.0001). Nosignificant difference was detected when all the groups were compared with respect to AST, ALT and GGT activities before and after the treatment (P>0.05). Our results showed that VCD may contribute to the sterilization of male dogs.
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