Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2025Protective effect of tarantula cubensis extract on ischemia-reperfusion injury in rat ovaries: Immunohistochemical, biochemical and histopathological evaluations
Paksoy, Zahid; Ozturk Sarikaya, Sevim Beyza; Yumusak, Nihat
Ischemia-reperfusion (IR) injury is a common pathology affecting the ovary. This study aimed to investigate the effect of Tarantula cubensis extract (TCE) on ovarian IR injury. Forty-two female rats were used and randomly divided into 7 groups: Control, Sham, IR, Ischemia+TCE+Reperfusion, TCE+IR, Ischemia+Ethanol+Reperfusion and Ethanol+IR. The control and sham groups did not receive any treatment. All other groups were subjected to 3 h of ischemia + 3 h of reperfusion. A single dose of TCE (0.2 ml Theranekron) was administered subcutaneously either before or after ischemia in the treatment groups. In the last 2 groups, ethanol was applied before or after ischemia. After reperfusion, blood samples were collected and analyzed for biochemical parameters. Additionally, the ovarian tissue was evaluated histopathologically and immunohistochemically. The IR group exhibited more marked hyperemia, hemorrhage, and necrosis. Both treatment groups showed histopathological improvement. Moreover, the recovery rate was greater in the animals given theranekron before ischemia. IR injury caused oxidative stress in the rats. However, Theranekron did not significantly reduce the MDA levels (p > 0.05). On the other hand, SOD levels were significantly increased in the treatment groups (p < 0.01). Compared to the control and sham groups, the IL-1β, IL-6 and TNF-α levels were very high in the IR group. Conversely, the immunoreactivity of these cytokines was further reduced in the animals given theranekron before ischemia. Our findings suggest that TCE may be beneficial in the treatment of ovarian IR damage, especially when Theranekron is given before ischemia.
- MakaleAçık Erişim2023Relationship of progranulin response with some physio-biochemical parameters in Akkaraman sheep
Bayraktar, Buelent; Tekce, Emre; Genç, Murat; Kaya, Hacer; Özcan, Gulbahar Boyuk; Aydemir, Seyma; Kotan, Goksad Cemil; Takma, Cigdem; Ürüsan, Hilal; Aksakal, Vecihi
Progranulin (PGRN) is a multifunctional protein that is critical in the retention of embryos in the endometrium of the uterus and the protection of cervical tissue during fetal growth. In case of PGRN release and dysfunction, cervical tissue may remodel (regeneration) and lead to the risk of abortion. Serum PGRN level in sheep and its variation according to different physiological periods and its relationship with some biochemical parameters are unknown. In this study, the relationship between early lactation, early and late pregnancy serum PGRN levels and some biochemical parameters in Akkaraman sheep was investigated for the first time. In the literature search, no similar studies were found on the relationship between early lactation, early and late pregnancy serum PGRN levels and some biochemical parameters in Akkaraman sheep. The research was carried out on 90 Akkaraman sheep reared in the same herd under the same care and feeding conditions. In the study, Akkaraman ewe were divided into 3 groups as early lactation, early pregnancy and late pregnancy. 1st group is early lactation period ewe (first 60 days of lactation), (n = 30), 2nd group is in the first period of pregnancy (on the 0-90th day of the first pregnancy (n = 30)), 3rd group is in the late pregnancy of pregnancy (100-150 days of pregnancy (n = 30)) was created. Blood samples from vena jugularis were taken before morning feeding from the animals that formed the study groups. PGRN levels in serum were determined by ELISA method. In addition, glucose, calcium (Ca), magnesium (Mg), phosphorus (P), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), very low density lipoprotein (VLDL) levels were analyzed in biochemistry autoanalyzer. As a result of the study, lactation and early pregnancy and late pregnancy mean serum PGRN levels were determined as 1.93, 1.78, 1.33 ng mL-1, respectively. The serum PGRN, glucose, Ca, Mg, P, TC, TG, LDL, HDL and VLDL levels of sheep with late pregnancy, early lactation and early pregnancy periods, which constitute the study groups, were found to be statistically significant (P<0.05). Our results have shown that due to the physiological role of PRGN in keeping the embryo in the endometrium of the uterine and in preserving the cervical tissue during fetal growth, there may be a useful parameter in the pursuit of the pregnancy process.
- MakaleYalnız künye2018The effects of 4-Vinylcyclohexene diepoxide on gene expression in dog testes
Paksoy, Zahid
In this study, the effects of 4-Vinylcyclohexene diepoxide (VCD) on the testicular gene expression in dogs were investigated. Eighteen male dogs were used as material and were randomly allocated to 3 groups. The first group (n=6) was administered sesame oil by intraperitoneal (i.p.) route once a day for 8 days, and this group was served as a control. The second group (n=6) received 80 mg/kg VCD and the third (n=6) group was administered VCD (i.p.) at a dose of 320 mg/kg once daily for 8 days. The testes were removed on following day the last VCD administration. The testes were immediately placed in the RNA stabilization solution and stored at -20°C. Later mRNAs were obtained from tissue samples. After RNA isolation, the cDNAs were synthesized. Finally, MasterMix was prepared and gene expression levels were examined at the real-time PCR. The expression of B cell lymphoma 2 (Bcl-2), tumor suppressor protein (tp53), Bax, CASP2, CASP3, CASP8, CASP9 and β-actin were investigated in the all groups. Cross-group comparisons were made by using student t test. The tp53 gene expression in the second group was found to be higher compared to the control group. It was determined that the expression of Bcl-2 was reduced in the testes of animals treated with high-dose VCD. CASP-3 was found to be more expressed and CASP-9 was down-regulated in the third group. In addition, CASP-8 gene expression was significantly reduced in both treatment groups. As a result, it can be concluded that expression changes in the tp53, Bcl-2, CASP-3, CASP-8 and CASP-9 genes in the testes of male dogs may cause apoptotic changes with the VCD application.
- MakaleYalnız künye2018Determination of Late Embryonic Death Rates by Pregnancy Associated Glycoproteins and Rectal Examination in Cows
Paksoy, Zahid
In this study, it was aimed to make the pregnancy diagnosis with the determination of glycoproteins (PAG) associated pregnancy after insemination in the cows, and to determine the late embryonic mortality rates. As material, 2919 head cows in different races and ages were used in farms and family businesses in Kahramanmaraş province. Milk sample was taken on the 25–30 day after artificial insemination from cows. The level of PAG in these milk samples was analyzed by using the Milk Pregnancy Test Kit for cows. This kit was used according to the manufacturer's instructions. During the study, it was determined that 1660 cows were pregnant, 188 were suspicious and 1071 were not pregnant. The pregnant animals were examined by rectal palpation on the 50th day when their pregnancy was continued. Controls performed on the 50th day of pregnant cows showed that 1623 cows were still pregnant and 37 cows had late embryonic deaths. As a result, early pregnancies can be accurately detected by measuring the level of PAG in the cow's milk using the Milk Pregnancy Test Kit. It was concluded that late embryonic mortality rates could be detected and that this analysis could be used routinely.
- MakaleAçık Erişim2018The effects of 4-vinylcyclohexene diepoxide on the testes of dogs; [Učinci 4-vinilcikloheksen diepoksida na testise pasa.]
Paksoy, Zahid; Kandemi̇r, Fatih M.; Gokhan, Nezihe; Ozkaraca, Mustafa
This study was aimed at investigating the effects of 4-vinylcyclohexene diepoxide (VCD) on testicular tissue in dogs. For this purpose, 30 mongrel male dogs were used and allocated to 5 groups randomly. The first group (n = 6), used as the control, was administered sesame oil by the intraperitoneal route (i/p). The VCD was administered i/p at doses of 80, 160, 240, 320 mg/kg to the second, third, fourth and the fifth groups (n = 6), respectively, once a day for 8 days. The dogs were surgically castrated on the day after the last VCD injection. The testes were removed immediately and fixed in 10% bouine solution. The testes were subjected to routine tissue processing and examined to determine the testicular damage in each testis. When compared to the control group, it was seen that the seminiferous tubule damage increased significantly in the treatment groups. Malondialdehyde levels reached the highest level in the fifth group, while superoxide dismutase, catalase, glutathione peroxidase activity and glutathione levels were higher in the control group (P<0.0001). Nosignificant difference was detected when all the groups were compared with respect to AST, ALT and GGT activities before and after the treatment (P>0.05). Our results showed that VCD may contribute to the sterilization of male dogs.
- MakaleYalnız künye2017The Effect of Flunixin Meglumine Administration after the Artificial Insemination on Pregnancy Rates in Cows
Paksoy, Zahid; Akar, Ibrahim
Flunixin meglumine is one of the most widely used non-steroidal anti-inflammatory agents in veterinary medicine. This drug is used on different days to prevent embryonic deaths and to increase pregnancy rates after insemination in cattle. The aim of this study was to evaluate the effect of flunixin meglumine administration on pregnancy rates at 14th day of post artificial insemination in cows. Holstein cows were used in the study (n=60). Animals were inseminated in natural oestrus and randomly divided into two groups after insemination. The first group (n=30) was administered 2 ml of saline postinsemination on the 14th day and this group constituted the control group. To the second group (n=30) was given flunixin meglumine at the dose of 2.2 mg / kg on the 14th day after insemination and this group constituted the treatment group. On the 45th day after insemination, animals were subjected to rectal examination and the pregnancy rates were determined. While 17 of 30 cows conceived in the control group, it was detected that 24 out of 30 cows treated with the flunixin meglumine was pregnant. This study showed that flunixin meglumine had a positive effect on pregnancy rates in cows. For this reason, it was concluded that flunixin meglumine administration after artificial insemination would be beneficial in maintaining the gestation.
- MakaleYalnız künye2016The Effect of Enrofloxacin on Myometrial Contractility in Bitches
Kurt, Akif Hakan; Paksoy, Zahid; Ergün, Yusuf
Enrofloxacin is a broad spectrum antibiotic used to treat bacterial infections. The aim of present study was to investigate the effect of enrofloxacin on Prostaglandin F2 alpha (PGF2 alpha) induced postpartum canine myometrium in vitro. Uterine tissues were obtained from bitches undergoing ovariohysterectomy in the postpartum period. Isolated myometrium strips suspended in organ bath filled with Kumagai solution and continuously gassed with 95% oxygen and 5% carbon dioxide (28°C). At the end of equilibration period, PGF2 alpha was added to the organ bath and the myometrial strips were observed to be contracted. Then the organ bath were administered solvent (n = 6), 2 mM (n = 6) and 5 mM (n = 10) enrofloxacin and % contraction was monitored. PGF2 alpha was given a second time and the contractions wererecorded after incubation with enrofloxacin. Solvent inhibited significantly myometrial contractions and enrofloxacin treated group showed a biphasic effect. The contractile response was significantly inhibited by 5 mM enrofloxacin compared to the control group. Our results show that high-dose enrofloxacin had inhibitory effects on the canine myometrial contractions. For this reason, enrofloxacin should be used combine with uterotonic agents in the metritis treatment.
- MakaleYalnız künye2015Effectiveness of levamisole and tarantula cubensis extract in the treatment of teat Papillomatosis of cows
Paksoy, Zahid; Gülesci, Nuri; Kandemir, Fatih Mehmet; Dinçel, Güngör Çagdas
Papillomatosis is widespread in cattle and is often observed on the skin of udders. For treatment, anthelmintic, immunomodulator and homeopathic drugs are administered. The objective of the study was to examine the therapeutic effect of levamisole and tarantula cubensis extract in the treatment of papilloma observed in the teats of cows. Sixteen Holstein cows between the age of 2-3 years were used as study material. The cows with Papillomatosis were randomly assigned into two equal groups. The animals in the first group (n=8) were treated with intramuscular double doses of levamisole (5 mg/kg) applied one week apart. The second group of animals (n=8) was treated with two subcutaneous doses of 10 ml of Tarantula cubensis extract one week apart. Four animals in the first group recovered while 8 animals in the second group recovered. The total serum oxidant levels of the animals treated with Tarantula cubensis extract and levamisole on the 15th day was found to be significantly lower than the serum levels of same animals on day 0 of the treatment (P<0.05). The results of this study revealed that tarantula cubensis extract applied in normal therapeutic doses was more effective in the treatment of teat papillomatosis than levamisole.