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- MakaleAçık Erişim2021Scopus SJRQ2Investigation of class 1 integrons with antibiotic resistance genes in multidrug-resistant acinetobacter baumannii strains and determination of plant extract effects on multidrug-resistant isolates
Saral, Ayşegül; Cinemre, Sedanur; Okumuş, Funda; Akar, Zeynep; Çiçek, Ayşegül Çopur; Düzgün, Azer Özad
This study aimed to investigate the presence of resistance genes in multidrug-resistant A. baumannii isolates as well as to determine the antibacterial activity of selected plant extracts against isolates. 41 strains were isolated from various clinical samples. PCR tests were performed using the primers. Methanol was used as solvent for the preparation of the plant extracts. MIC values of the plant extracts were determined by the broth microdilution method. The blaOXA-23, blaCTX-M-1, blaCTX-M-2, blaGES genes and Class 1 integrons were detected in five isolated strains. The lowest MIC value (2.25 mg/mL) was determined for the Echinacea purpurea extract, while the highest MIC value (50 mg/mL) was determined for the Morus alba extract. Determination of the antibacterial effect of plants extracts used in the study against A. baumannii isolates shows the importance of screening the antibacterial activity of plants in the fight against antibiotic resistance.
AtıfScopus1WoS1OpenAlex3
İncele - MakaleYalnız künye2018Scopus SJRQ3ANTIMICROBIAL AND ANTIOXIDANT SCREENING, SYNERGY STUDIES OF HELICHRYSUM CHIONOPHILUM EXTRACTS AGAINST TO RESISTANT MICROBIAL STRAINS
Ozcan, Kadriye; Acet, Tuba
In this study, the antimicrobial activities and antioxidant properties of five different extracts of Helichrysum chionophilium plants from Gumushane/Turkey were investigated. To determine the antimicrobial activity disc diffusion and microdilution methods were used against to fifteen microorganisms. The effect of plant extracts and antibiotic combinations against to vancomycin-resistant Enterococcus faecium and methicillin-resistant Staphylococcus aureus were investigated as well. Total phenolic and flavonoid contents were determined by spectrophotometric techniques. Antioxidant properties of plant extracts evaluated by free radical scavenging assays which ABTS and DPPH. The plant ethyl acetate extract showed the highest level of total phenolic (423.01 +/- 0.8 mg GAE/g extract) and flavonoid contents (100.18 +/- 1.4 mg QE/g extract). Similarly, ethyl acetate extract exhibited the highest level of DDPH (IC50: 67.44 +/- 0.9 mu g/ml). However, hexane extract exhibited the highest level activity of ABTS (IC50: 55.20 +/- 1.3 mu g/ml). The highest MIC value was determined as 8 mu g/ml against to Pseudomonas aeruginosa on ethyl acetate extract. Besides, all of extracts exhibited antimicrobial activity on at least one test organism. Plant extracts with some antibiotic combinations generated synergies or partial synergic activity against to VREF and MRSA. These findings revealed that the plant has strong antimicrobial and antioxidant properties. Finally, using the plant extracts and antibiotic combinations might be a good tool in fighting with antibiotic resistance strains.
AtıfWoS7İncele - MakaleYalnız künye2017Scopus SJRQ4Molecular docking analysis and determination of minimum inhibition concentration of VIM-38/R228S
Duzgun, Azer Ozad; Saral, Aysegul
Objective: The aim of this study was to determine the effects of residues at 228th positions of VIM-38 on minimum inhibition concentration (MIC) and molecular docking analysis. Materials and methods: blaVIM-38 gene was cloned into expression vector pET100/D-TOPO. Then pET100/D-TOPO-VIM-38 was used to generate the R228S mutation by site-directed mutagenesis. The mutant was transformed into E. coli Dh5α. Changes in the activity of mutation R228S was determined by E-test. Molecular docking analysis of the binding affinity of meropenem and imipenem with VIM-38 and VIM-38 R228S was performed using AutoDock Vina_1_1_2. Results: According to E-test results, pET100/D-TOPO-VIM-38 and pET100/D-TOPO-VIM-38/ R228S have same MIC value for amoxicillin, ticarcillin/clavulanic acid, ampicillin and ticarcillin. It was determined decrease in pET100/D-TOPO-VIM-38/ R228S of MIC for aztreonam, amikacin and cefixine. There are 3-fold deference in MIC between pET100/D-TOPO-VIM-38 and pET100/D-TOPO-VIM-38/ R228S for cefoxitin. pET100/D-TOPO-VIM-38 has 0.5 µg/ml and pET100/D-TOPO-VIM-38/ R228S has 0.125 µg/m for imipenem, we observed 4-fold deference. Also, there is about 170-fold deference between pET100/D-TOPO-VIM-38 (16 µg/ml) and pET100/D-TOPO-VIM-38/ R228S (0.094 µg/ml) for meropenem. VIM-38 and VIM-38 R228S have very close to the negative low free energies of binding of the meropenem and imipenem. Conclusion: These results showed that substitution (R228S) slightly have effect on enzyme activity.