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- MakaleYalnız künye2026Scopus SJRQ1Effects of sunlight ripening process on the biological activities of persimmon (Diospyros kaki L.) peel and pulp extracts
Kardil, Uğur; Akar, Zeynep; Düzgün, Azer Özad
The aim of the study was to evaluate the biological properties of the peel and pulp extracts of persimmons, with consideration of the impact of different ripening conditions (B: on-branch ripened and S: sunlight ripened). The data demonstrated that the sunlight ripened persimmon peel (S. peel) extract exhibited a higher total phenolic and flavonoid content, with 157 and 7.3 µg/mL GAE respectively. The S. peel extract exhibited the highest antioxidant activity, exhibiting 1.1578 mg/mL IC50, 54.095 and 0.0262 µM Trolox equivalent (TE)/g fresh weight (FW), as determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assay, ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant capacity (CUPRAC) respectively. LC-MS/MS analysis revealed that the peel and pulp phenolic composition of persimmon ripening process different conditions changed. The activity of protocatechuic, chlorogenic, caffeic and ferulic acids identified in persimmon extracts was determined by HPLC-DAD-FRAP assay. ICP-OES analysis revealed that potassium exhibited the highest mineral content of persimmon, with values ranging from 575.680 to 2031.360 mg/kg. The S. peel extract was more inhibitory activity against bovine carbonic anhydrase (BCA) and α-glucosidase then other extracts. Moreover, the S. peel extract was found to demonstrate the highest level of antibiofilm activity, reducing the strong biofilm formation capacity of the A. baumannii isolate to negative, both qualitatively and quantitatively. The study suggests that the ripening of persimmon fruit in sunlight may increase biological activity and may be useful in the prevention and treatment of diseases associated with oxidative damage, diabetes and bacterial infections.
- MakaleAçık Erişim2026Scopus SJRQ3Green Synthesis and Bioactivities of Silver Nanoparticles Derived from Mercurialis annua L. Extract
Salad, Aisha Mohamud; Akar, Zeynep
Biogenic synthesis using plant materials provides a sustainable alternative to classical nanoparticle fabrication, minimizing chemical hazards and decreasing energy input. In this study, silver nanoparticles (AgNPs) were prepared using an aqueous extract of Mercurialis annua and characterized. Physicochemical properties of the AgNPs were confirmed using ultraviolet–visible spectroscopy (UV–Vis), Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), and transmission electron microscopy coupled with energy-dispersive X-ray (TEM–EDX), which collectively indicated crystalline, spherical silver nanoparticles stabilized by extract-derived functional groups. Subsequently, the biological activities of both the nanoparticles and the extract were comparatively evaluated using antioxidant tests, including cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic content (TPC), and total flavonoid content (TFC), as well as enzyme inhibition tests targeting α-glucosidase and carbonic anhydrase. Liquid chromatography-mass spectrometry profiling identified 31 phenolic constituents in the extract, with ferulic, caffeic, salicylic, and 4-hydroxybenzoic acids detected in high abundance. Overall, the biosynthesized AgNPs demonstrated higher antioxidant performance and stronger inhibitory effects on both enzymes compared with the crude extract. These results indicate that M. annua-based AgNPs hold considerable promise sustainable and effective bioactive agents for future biomedical and biotechnological applications.
- MakaleAçık Erişim2025Scopus SJRQ1Unveiling the bioactive potential of Scabiosa rotata M. Bieb fruits: a landmark study on cytotoxic, antioxidant, and antibacterial activities through liquid chromatography-mass spectrometry profiling and molecular docking
Yildirim, Aybeniz; Celep, Inci Kurt; Gül, Abdulkadir; Eter, Özge Soylu; Baǧcl, Eyüp
The aim of this study was to investigate the antioxidant, cytotoxic, and antibacterial activities of ethanol extract (SRE) and its hexane (SRH), chloroform (SRC), ethyl acetate (SREA), and aqueous ethanol (SRAE) sub-extracts obtained from the fruits of Scabiosa rotata M. Bieb. Molecular docking studies were also performed to evaluate the interactions of the most active sub-extract with the human mitochondrial ABC transporter (ABCB10) protein, plays an important role in cellular oxidative stress regulation and mitochondrial function. Total phenolic (TPC) and flavonoid (TFC) contents and antioxidant radical scavenging activities (1, 1-diphenyl-2-picryl hydrazyl [DPPH] and 3-ethylbenzothiazoline-6 sulfonic acid [ABTS]) of all extracts were determined. Cytotoxic activity was evaluated on different cell lines, such as HepG2 (liver cancer), MCF-7 (breast cancer), and HeLa (cervical cancer) cells. Antibacterial activity was tested against Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, and Staphylococcus aureus. SREA showed the best antioxidant activity with IC50 values of 54.20 μg/ml and 48.56 μg/ml for DPPH and ABTS, respectively, and also had high TPC (499.06 mg GA/g) and TFC (327.45 mg QE/g). Furthermore, SREA showed significant cytotoxicity comparable to cisplatin with IC50 values of 51.29, 51.41, and 52.89 μg/ml for HepG2, MCF-7, and HeLa cells, respectively. However, none of the extracts showed antibacterial activity. The analysis using liquid chromatography-mass spectrometry (LC/MS-MS) on SREA found that the main phenolic compounds are chlorogenic acid, hesperidin, quinic acid, and isoorientin. Molecular docking of the major compounds showed that chlorogenic acid (2.21 mM) exhibited the strongest binding affinity, followed by hesperidin (5.83 mM), quinic acid (8.86 mM), and isoorientin (13 mM). These findings indicate the potential of SREA to be used as a natural antioxidant and anticancer agent.
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İncele - MakaleYalnız künye2025Scopus SJRQ3Inhibition Effect of Cordyceps sinensis (C. sinensis) Mycelium on Some Metabolic Enzymes and Investigation of Antioxidant Properties
Balcı, Neslihan
Cordyceps sinensis (CS), a traditional medicinal fungus, is a rich source of bioactive metabolites with pharmacological potential. This study investigated the inhibitory effects of CS mycelium on human carbonic anhydrase isoenzymes I and II (hCA I and hCA II) and acetylcholinesterase (AChE), as well as its antioxidant properties. Enzyme inhibition was assessed using spectrophotometric methods, whereas antioxidant capacity was evaluated by DPPH, ABTS, CUPRAC, FRAP, and Fe3⁺-reducing assays. LC-MS/MS analysis identified key secondary metabolites, including apigenin, apigenin-7-glucoside, (+)-taxifolin, dihydrokaempferol, p-coumaric acid, salicylic acid, and L-theanine, with apigenin as the major constituent. CS strongly inhibited hCA I (IC50 = 1.11 µg/mL) and hCA II (IC50 = 1.77 µg/mL), exceeding the potency of acetazolamide, and also showed relevant AChE inhibition (IC50 = 8.00 µg/mL), comparable to tacrine. Antioxidant assays revealed moderate DPPH activity but strong ABTS and CUPRAC reducing capacities, indicating the structural contributions of flavonoids and phenolic acids. These findings suggest that CS possess dual bioactivity through enzyme inhibition and antioxidant effects, highlighting its therapeutic potential against neurodegenerative and oxidative stress–related diseases.
- MakaleAçık Erişim2020Scopus SJRQ2Biological activities and phytochemical screening of ethanol extracts from adonis paryadrica (Ranunculaceae)
Ucuncu, Osman; Baltaci, Cemalettin; Akar, Zeynep; Duzgun, Azer Ozad; Cuce, Mustafa; Kandemir, Ali
In this study, secondary metabolite profiles of flower, leaf and root of Adonis paryadrica ethanolic extracts were performed by using a newly developed and validated LC-MS/MS method. For this purpose, the LC-MS/MS system was used for the quantitative and qualitative analysis of 37 phytochemicals. The main component of the flower, leaf and root extracts was the quinic acid (160, 111 and 64 mg/kg, respectively). Antioxidant activities were determined using methods such as DPPH (SC50, mg/mL), FRAP (µM TEAC), CUPRAC (µM TEAC), ABTS (SC50, mg/mL) and TPC (GAE, µg/mL). While the highest antioxidant activity value for FRAP, CUPRAC, ABTS and TPC methods was determined in the leaf part of the plant, this value was determined in the flower part for DPPH. The antibacterial effects of the plant extracts were determined by liquid microdilution and agar diffusion methods. As a result, antimicrobial effect of flowers, leaf and all sections of plant extracts were determined except plant root extracts which did not display any activity against bacteria. Antimicrobial activity of the plant flower extract was found to be higher especially against to P. vulgaris with 3.5 mg/mL MIC.
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