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- MakaleYalnız künye2026Germination as a Pre-isolation Modification Method for Common Buckwheat Protein Isolate
Gülbahar, Esma; Tunç, Merve Tuğçe; Akdoğan, Arda; Baltacı, Cemalettin; Sarıcaoğlu, Furkan Türker; Parlak, Mahmut Ekrem; Aydın, Engin; Odabaş, Halil İbrahim
This study investigated germination as a pre-isolation modification method to enhance the techno-functional and nutritional properties of common buckwheat (Fagopyrum esculentum Moench) protein. Germination was conducted at two different temperatures (20 and 30 °C) for 24, 48, and 72 h, and isolated proteins were analyzed for their chemical, structural, and functional properties. Results showed that the highest protein yield was achieved at 30 °C for 24 h, though durations exceeding 48 h reduced purity. SDS-PAGE and FTIR analyses confirmed that endogenous protease activity degraded high-molecular-weight proteins and increasing random coil proportions, indicating partial unfolding. SEM imaging revealed that germination transformed the dense protein surface into a porous morphology. Increasing germination time and temperature led to decreased lightness (L*) and hue angle, along with moderate increases in redness (a*), indicating notable changes in the visual appearance of the protein isolates. DSC results showed a decrease in thermal stability (Td) due to structural changes. In addition, germination significantly improved solubility, emulsifying activity, foaming capacity, and water/oil absorption, particularly at the 24 h of germination. Most notably, germination at 30 °C for 24 h increased in vitro protein digestibility from 75.67 to 82.19%. The results show that the germination is a promising approach to enhance the functionality and digestibility of buckwheat protein for potential application in food industry.
- MakaleYalnız künye2019EFFECT OF DIFFERENT GIBBERELLIC ACID DOSES TREATMENT ON SEED GERMINATION OF WILD SAINFOIN (ONOBRYCHIS VICHFOLIA)
Okcu, Melih; Topaloglu, Fatma Nil
Sainfoin (Onobrychis viciifolia) subjected to a range of studies relating to agronomy, environmental preferences, crop protection and plant-microbial interactions, is an important forage species in agriculture, that be prefered by farmers than other forage legumes. This fact has give importance to breeding of this plant for the development of diverse varieties and so for this purpose many wild forms are cultivated. The most important problem in sainfoin cultivation is poor germination problem in wild species. With the aim of solving this problem, we carried out to evaluate the response of wild sainfoin seeds to the application of different gibberellic acid (GA3) (a germination hormone) concentration in vitro conditions. Five levels of gibberellic acid (100, 250, 500, 1000 and 1500 ppm) was used in the test. Seed coat was removed and the seeds were placed on Murashige&Skoog (MS) medium supplemented with gibberellic acid (GA3) and kept under these conditions for 46 days. Seed germination percentage and germination associate parameters; speed of germination, mean germination time, mean daily germination, peak value and germination value, were calculated. The experiment was carried out using complete block design with three replications. According to the results obtained from different GA3 application medium, the highest germination percentage 48,89 recorded at in medium containing 250 ppm GA3 whereas in control has recorded 42,22 at the end of the 46th day. Other concentrations, 100, 500,1000 and 1500 ppm, achieved a reduction in seed germination compared to control, respectively 28,15, 15,56, 24,44, 34,81. Not only in germination percentage result, but also in other germination associate parameters, the addition of 250 ppm gibberellic acid to Murashige and Skoog medium increased the values compared to the control and ensured a rapid increase and peak between the 8th and 12th days in all parameters.