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- MakaleYalnız künye2021Simultaneous separation and preliminary purification of anthocyanins from Rosa pimpinellifolia L. fruits by microwave assisted aqueous two-phase extraction
Odabaş, Halil İbrahim; Koca, Ilkay
Rosa pimpinellifolia L. fruits (RPF) have great industrial potential due to the high presence of anthocyanins. In this work, microwave-assisted aqueous two-phase extraction (MA-ATPE) was applied to simultaneous extraction and purification of anthocyanins from RPF with ethanol/ammonium sulfate aqueous two-phase system (ATPS). MA-ATPE process was optimized by single-factor experiments and response surface methodology. The optimal anthocyanin yields were achieved in conditions of 0% HCl (w:w), 26.85% ethanol (w:w), 19.15% ammonium sulfate (w:w), liquid to solid ratio 40, 17.29 min, 60 °C and 400 W. Predicted anthocyanin yield of MA-ATPE at optimum conditions were determined as 1373.04 mg cyanidin 3-glucoside equivalent/g dry RPF. The purity of anthocyanin rich extract obtained by MA-ATPE was 1.65-fold greater than microwave assisted extraction using 80% ethanol (MAE). The compositional characterization of anthocyanins were carried out with HPLC-QTOF-MS. MA-ATPE had no obvious changes on anthocyanin composition when compared to MAE. Results indicated that the MA-ATPE could be a promising method for the extraction and preliminary purification of anthocyanins from different plant materials.
- MakaleYalnız künye2021Single-step recovery of pectin and essential oil from lemon waste by ohmic heating assisted extraction/hydrodistillation: A multi-response optimization study
Tunç, Merve Tuğçe; Odabaş, Halil İbrahim
The main products obtained from citrus wastes are pectin and essential oil. In this work, ohmic heating assisted extraction/hydrodistillation (OHAE/H) was applied for the simultaneous recovery of pectin and essential oil from lemon waste. Optimum OHAE/H conditions to maximize the yield of pectin and essential oil were determined with response surface methodology. Three independent variables, namely liquid to solid ratio (w:v), extraction/hydrodistillation time (min) and voltage gradient (V/cm) were varied to obtain maximum pectin and essential oil yield. Optimum OHAE/H conditions were determined as 8.7:1 liquid to solid ratio, 58.4 min extraction/hydrodistillation time and 14.2 V/cm voltage gradient. The predicted pectin and essential oil yields at optimum conditions were 16.58 and 3.62 g/100 g lemon waste, respectively. Data analysis showed that all of the independent variables significantly affected the pectin and essential oil yield. In the verification study, close agreement was found between predicted and experimental values. Conventional heating extraction/hydrodistillation (CHE/H) were conducted to evaluate the effectivity of OHAE/H. With OHAE/H process, the yields of the pectin and essential oil were significantly increased when compared to CHE/H. Limonene concentration of the essential oil produced with OHAE/H was higher than the essential oil produced by CHE/H. Results demonstrate that the single-step OHAE/H could be an effective method for simultaneous extraction and hydrodistillation of pectin and essential oils.