Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2022Assessing ursolic acid contents of some commonly consumed herbs grown in Turkey
Koca, Ilkay; Zannou, Oscar; Ipekci, Burak; Odabaş, Halil İbrahim
Ursolic acid is a triterpenoid compound in the plant kingdom and has important biological functions. In this study, ursolic acid contents of nine herbs including Anethum graveolens (dill), Camellia sinensis L. (green tea), Lepidium sativum L. (garden cress), Ocimum basilicum L. (sweet basil), Petroselinum crispum L. (parsley), Rosmarinus officinalis L. (rosemary), Salvia officinalis L. (sage), Thymus vulgaris L. (thyme) and Urtica dioica L. (nettle) were determined by High-Performance Liquid Chromatography (RP-HPLC-UV). Based on the results from the analysis of herbs, ursolic acid contents varied between 0.20% and 7.11%. The ursolic acid content was highest in rosemary and sage (7.11±0.20% and 6.63±0.27%, respectively), while the lowest content of ursolic acid was in nettle (0.20±0.10). It is expected from the results of this work to contribute to the knowledge on the nutritional value of commonly consumed herbs in Turkey and encourage their consumption as functional foods.
- Proje2022Buğday Rüşeyminden Ultrason Destekli Üçlü Faz Ayırma Yöntemi ile Yağ, Protein ve Polisakkaritlerin Eşzamanlı Olarak Ekstraksiyonu
Saricaoglu, Furkan Turker; Koca, Ilkay; Tekgüler, Belkis; Odabaş, Halil İbrahim
Bu çalışmada, bir değirmencilik endüstrisi yan ürünü olan buğday rüşeyminden yağ, protein ve polisakkaritlerin eşzamanlı olarak ekstraksiyonu için ultrason destekli üçlü faz ayırma (UDÜFA) yöntemi kullanılmıştır. Yağ, protein ve polisakkarit verimleri üzerine amonyum sülfat konsantrasyonu, t-bütanol oranı, pH, ekstraksiyon süresi, genlik ve vurgu aralığı değişkenlerinin etkileri tek faktör analizleri ile belirlenmiştir. Tek-faktör analizlerine dayanarak, maksimum seviyede yağ, protein ve polisakkarit ekstraksiyonu için yanıt yüzey yöntemi kullanılarak UDÜFA süreci optimize edilmiştir. Optimum ekstraksiyon koşulları %30 amonyum sülfat konsantrasyonu, 1 t-bütanol oranı, 4.49 pH, 15 dakika ekstraksiyon süresi, 35 °C ekstraksiyon sıcaklığı, % 65.04 genlik ve % 51.11 vurgu aralığı olarak belirlenmiştir. Belirlenen optimum koşullarda tahminlenen yağ, protein ve polisakkarit verimi sırasıyla %8.78, %17.98 ve %1.58 olarak belirlenmiştir. Elde edilen modelin doğrulanması çalışmasında tahminlenen ve deneysel verim değerleri arasında yüksek uyum bulunmuştur. UDÜFA yöntemi, üçlü faz ayırma (ÜFA) ve geleneksel yağ, protein ve polisakkarit ekstraksiyon yöntemleri karşılaştırılmıştır. UDÜFA yönteminin kullanımı ile ÜFA ve geleneksel yöntemlere göre daha yüksek yağ, protein ve polisakkarit verimi elde edilmiştir. UDÜFA, ÜFA ve geleneksel yöntemlerle elde edilen yağ, protein ve polisakkaritlerin fizikokimyasal, yapısal, fonksiyonel ve biyoaktif özellikleri karşılaştırılmıştır. Genel olarak UDÜFA, ÜFA ve geleneksel hekzan ekstraksiyonu ile elde edilen yağların fizikokimyasal özellikleri benzerdir. Yağların biyoaktif özellikleri UA-TPP ve TPP yönteminde geleneksel hekzan ekstraksiyonu yöntemine göre daha iyidir. UDÜFA ve ÜFA yöntemleri ile elde edilen proteinlerin fonksiyonel özellikleri, geleneksel alkali ortamda çözündürme/izoelektrik noktada çöktürme yöntemi ile elde edilen proteinden daha iyidir. UDÜFA ve ÜFA yöntemleri ile elde edilen polisakkaritlerin biyoaktif özellikleri (antioksidan aktivite, glukoz absorbsiyonu kapasitesi ve safra asidi bağlama kapasitesi) geleneksel sıcak su ekstraksiyonuna göre daha iyidir. Sonuçlar UDÜFA ve ÜFA yöntemlerinin buğday rüşeyminden yağ, protein ve polisakkaritlerin tek aşamalı ekstraksiyonu için etkili yöntemler olduğunu ortaya koymaktadır.
- MakaleYalnız künye2021Simultaneous separation and preliminary purification of anthocyanins from Rosa pimpinellifolia L. fruits by microwave assisted aqueous two-phase extraction
Odabaş, Halil İbrahim; Koca, Ilkay
Rosa pimpinellifolia L. fruits (RPF) have great industrial potential due to the high presence of anthocyanins. In this work, microwave-assisted aqueous two-phase extraction (MA-ATPE) was applied to simultaneous extraction and purification of anthocyanins from RPF with ethanol/ammonium sulfate aqueous two-phase system (ATPS). MA-ATPE process was optimized by single-factor experiments and response surface methodology. The optimal anthocyanin yields were achieved in conditions of 0% HCl (w:w), 26.85% ethanol (w:w), 19.15% ammonium sulfate (w:w), liquid to solid ratio 40, 17.29 min, 60 °C and 400 W. Predicted anthocyanin yield of MA-ATPE at optimum conditions were determined as 1373.04 mg cyanidin 3-glucoside equivalent/g dry RPF. The purity of anthocyanin rich extract obtained by MA-ATPE was 1.65-fold greater than microwave assisted extraction using 80% ethanol (MAE). The compositional characterization of anthocyanins were carried out with HPLC-QTOF-MS. MA-ATPE had no obvious changes on anthocyanin composition when compared to MAE. Results indicated that the MA-ATPE could be a promising method for the extraction and preliminary purification of anthocyanins from different plant materials.
- MakaleYalnız künye2020Process for production of microencapsulated anthocyanin pigments from Rosa pimpinellifolia L. fruits: optimization of aqueous two-phase extraction, microencapsulation by spray and freeze-drying, and storage stability evaluation
Odabas, Halil Ibrahim; Koca, Ilkay
Rosa pimpinellifolia L. fruits (RPF) are promising source of anthocyanin pigments. The objectives of this study were to optimization of the aqueous two-phase extraction (ATPE) process of anthocyanin from RPF and microencapsulation of anthocyanin-rich RPF extract. The optimal ATPE conditions were as follows: 0% HCl, 30% ethanol, 19% ammonium sulfate, and liquid to solid ratio 51.71, 97.71 min, and 30 degrees C extraction temperature. Predicted anthocyanin yield at the optimum conditions was 1578.90 mg cyanidin 3-glucoside equivalent/100 g dry fruit. ATPE resulting in 1.80-fold increase in the purity of anthocyanins when compared to conventional solvent extraction (CSE). The composition of the anthocyanins were determined with HPLC-QTOF-MS. Freeze-drying and spray-drying methods were employed for the production of microencapsulated anthocyanin pigments. The half times of microencapsulated anthocyanins at 4, 25 and 37 degrees C were determined as 12.16, 6.60 and 3.12months for freeze-dried microcapsules, and 16.50, 9.24 and 4.29 months for spray-driedmicrocapsules, respectively.
- MakaleYalnız künye2016Application of response surface methodology for optimizing the recovery of phenolic compounds from hazelnut skin using different extraction methods
Odabaş, Halil İbrahim; Koca, Ilkay
Hazelnut skin which is a rich source of phenolic compounds is a by-product of hazelnut roasting process. In present study, ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE) and supercritical carbon dioxide extraction (SCE) methods were applied for recovery of phenolic compounds from hazelnut skin. Response surface methodology was used to estimate optimum extraction conditions. Extraction studies were performed according to Box-Behnken Design. Temperature (UAE)/power (MAE), time and ethanol concentration were independent variables for UAE and MAE. The independent variables selected for SCE were temperature, time and pressure. The optimum extraction conditions were 45 min and 67.2–67.6% ethanol concentration, 600 W, 6 min and 55.03–56.23% ethanol concentration and 42.72–49.10 °C, 59.83–60.00 min and 10.01–11.48 bar, for UAE, MAE and SCE respectively. Total phenolic content, FRAP and 1/EC50 values at the optimum conditions were 122.99–123.01 mg GAE/g, 612.20–613.25 mmol Fe(II)/g and 4.36 mL/mg for UAE, 111.53–111.55 mg GAE/g, 582.44–582.52 mmol Fe(II)/g and 2.48 mL/mg for MAE and 69.59–72.64 mg GAE/g, 426.25–465.52 mmol Fe(II)/g and 2.18–2.27 mL/mg for SCE, respectively. Maceration was performed for comparison with novel methods. UAE was found to be the best method of the extraction of phenolic compounds from hazelnut skin with the highest total phenolic content and antioxidant activity values.