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- MakaleYalnız künye2026Scopus SJRQ4Covalent Immobilization of Glutathione-S-Transferase onto Magnetic Iron Nanoparticles via Epichlorohydrin Spacer Arm
Kemal Caglar, M.; Gulesci, Nuri; Bilgin, Ramazan; Yucebilgic, Guzide
This study examined the synthesis of Fe₃O₄ magnetic nanoparticles (MNPs) and the covalent immobilization of glutathione-S-transferase (GSTs) onto these nanoparticles using an epichlorohydrin (ECH) spacer arm, as well as the optimal reaction conditions for both free and immobilized enzymes and the reusability of the immobilized enzyme. The optimal pH values for free and immobilized enzymes were established as 7.0 and 6.0, respectively. It was found that the ideal temperature for both free and immobilized enzymes was 37 and 40°C, respectively. Under the optimum conditions, the values of V_max for glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) substrates of free GSTs enzyme were measured as 204.8 U/mg prot. and 194.3 U/mg prot., respectively. The immobilized GSTs enzyme's CDNB and GSH substrates had V_max values of 33.29 U/mg prot. and 33.08 U/mg prot., respectively. The free GSTs enzyme's CDNB and GSH substrates have K_m values of 0.201 mM and 0.1873 mM, respectively. GSH and CDNB have K_m values of 0.3042 mM and 0.2523 mM, respectively. At various temperatures, we contrasted the thermal stability of free and immobilized GSTs. The immobilized GSTs enzyme retained 50% of its activity after 20 reuses. After 30 days of storage at 25°C and 4°C, it was discovered that 18% and 32% of the free GSTs enzyme activity were preserved, and 30 days of storage at 25°C and 4°C resulted in 20% and 41% preserved enzyme activity for the immobilized enzyme, respectively.
- MakaleYalnız künye2025Scopus SJRQ2Chemical Composition and Bioactivities of Sideritis libanotica Labill subsp. Libanotica: LC–HRMS Phytochemical Profiling, Mineral Content, Antioxidant, Antibacterial, and Neuroprotective–Antidiabetic Enzyme Inhibitory Potentials
Öztürk Sarıkaya, Sevim Beyza; Zehiroğlu, Cuma
Sideritis libanotica Labill. subsp. libanotica (SLL) is an herbal tea used for colds, coughs, and digestive system disorders. SLL has higher antioxidant activity in methods (µg/mL) like 1,1-diphenyl-2-picrylhydrazyl (DPPH•) (40.912 ± 0.170), ABTS•+ (14.61 ± 0.331) scavenging activity, and Fe3+ (0.872 ± 0.052) and Cu2+ (0.840 ± 0.013) reduction capacity compared to other Sideritis species. It is rich in essential minerals, such as Ca, Mg, and P, with the highest content of mineral K (14 203.97 mg/kg). Zone values in the range of 9–11 mm were obtained from Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Pseudomonas putida, and Klebsiella pneumoniae strains, which is an indicator that the plant has antibacterial activity. Among 33 secondary metabolites, sinapinic acid, rutin, and hispidulin have high values, whereas the one with the highest value is verbascoside (274 483 mg/kg), a pharmacologically active compound. It showed an inhibitory effect (mg/mL) on some enzymes targeting neurodegenerative and metabolic disorders. (IC50; acetylcholinesterase [AChE]: 0.065, butyrylcholinesterase [BChE]: 0.086, α-glucosidase: 0.014).
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İncele - MakaleYalnız künye2023Scopus SJRQ3Determination of the Antioxidant Capacity of Puerarin and Its Effect on Cholinesterases by in vitro and in silico Methods
İnan Ergün, Aslı; Topal, Meryem
Puerarin, which was used as a natural isoflavone in this study, was obtained from the Kudzu plant. The inhibitory effect of puerarin on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes was investigated. The IC50 values of AChE (177.73 nM) and BChE (315.07 nM) were determined. The binding mechanism of puerarin, which has a high inhibitory effect on the active site of the enzyme, was demonstrated by the molecular docking method. The antioxidant activity was evaluated for the same molecule. For this purpose, Fe3+-Fe2+ reducing capacity, ABTS radical scavenging activity, CUPRAC method, DPPH⋅ scavenging activity and DMPD radical scavenging method were used. The percentage (%) inhibition amount of puerarin molecule was calculated as 99.33 % for ABTS radical scavenging activity, which is the more preferred method for natural products. The absorbtion value of iron reduction of Puerarin was 0.122±0.010, and the value of copper reduction was 0.633±0.026. Our research efforts include uncovering the potent antioxidant potential of puerarin and, simultaneously, a comparative study of tacrine, primarily emphasizing its inhibitory effects against AChE and BChE enzymes. The puerarin is expected to be a guide for drug discovery against many problems, especially oxidative cell damage in Alzheimer's disease, mitochondrial dysfunction (MD), cancer, and cardiovascular diseases.
AtıfScopus11WoS5OpenAlex9İncele - MakaleYalnız künye2017Scopus SJRQ2Isolation and Characterization of Thermophilic Bacteria from Geothermal Areas in Turkey and Preliminary Research on Biotechnologically Important Enzyme Production
Baltaci, Mustafa Ozkan; Genc, Berna; Arslan, Sumeyya; Adiguzel, Gulsah; Adiguzel, Ahmet
In this study, the isolation, identification and characterization of the thermophilic bacteria from different hot springs in Turkey were carried out by conventional (morphological, physiological and biochemical tests) and molecular methods (fatty acid methyl esters, GTG5-PCR and 16S rRNA sequencing). These thermophilic bacteria were then tested for their capability to produce enzymes such as lipase, protease, amylase and cellulase. O20 strain is a novel species according to identification studies. All of its isolates were capable of producing industrially valuable enzymes based on screening. In fact, most of them could produce at least two of these enzymes.
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