Isolation and Characterization of Thermophilic Bacteria from Geothermal Areas in Turkey and Preliminary Research on Biotechnologically Important Enzyme Production

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Özet

In this study, the isolation, identification and characterization of the thermophilic bacteria from different hot springs in Turkey were carried out by conventional (morphological, physiological and biochemical tests) and molecular methods (fatty acid methyl esters, GTG5-PCR and 16S rRNA sequencing). These thermophilic bacteria were then tested for their capability to produce enzymes such as lipase, protease, amylase and cellulase. O20 strain is a novel species according to identification studies. All of its isolates were capable of producing industrially valuable enzymes based on screening. In fact, most of them could produce at least two of these enzymes.

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AlanDeğerDil
dc.contributor.authorBaltaci, Mustafa Ozkan
dc.contributor.authorGenc, Berna
dc.contributor.authorArslan, Sumeyya
dc.contributor.authorAdiguzel, Gulsah
dc.contributor.authorAdiguzel, Ahmet
dc.date.accessioned2026-10-22T10:23:27.482309944Z
dc.date.available2026-10-22T10:23:27.482309944Z
dc.date.issued2017
dc.description.abstractIn this study, the isolation, identification and characterization of the thermophilic bacteria from different hot springs in Turkey were carried out by conventional (morphological, physiological and biochemical tests) and molecular methods (fatty acid methyl esters, GTG5-PCR and 16S rRNA sequencing). These thermophilic bacteria were then tested for their capability to produce enzymes such as lipase, protease, amylase and cellulase. O20 strain is a novel species according to identification studies. All of its isolates were capable of producing industrially valuable enzymes based on screening. In fact, most of them could produce at least two of these enzymes.
dc.description.sponsorshipResearch Development Centre of Ataturk University
dc.description.sponsorship[2015/088]
dc.identifier.doi10.1080/01490451.2015.1137662
dc.identifier.eissn1521-0529
dc.identifier.endpage62
dc.identifier.issn0149-0451
dc.identifier.issue1
dc.identifier.scopus2-s2.0-84976571605
dc.identifier.startpage53
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/gumushane/15012
dc.identifier.volume34
dc.identifier.wosWOS:000393661600006
dc.language.isoen
dc.publisherTaylor and Francis Inc.
dc.relation.ispartofGeomicrobiology Journal
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject16S rRNA sequencing
dc.subjectenzyme
dc.subjectFAMEs
dc.subjectGTG5-PCR
dc.subjectthermophilic bacteria
dc.subject.classificationEnvironmental Sciences
dc.subject.classificationGeosciences, Multidisciplinary
dc.subject.classificationEnvironmental Sciences & Ecology
dc.subject.classificationGeology
dc.subject.otherTurkey
dc.subject.otherbiotechnology
dc.subject.otherenzyme activity
dc.subject.othergeothermal system
dc.subject.otherpolymerase chain reaction
dc.subject.otherresearch
dc.subject.otherRNA
dc.subject.otherthermophilic bacterium
dc.titleIsolation and Characterization of Thermophilic Bacteria from Geothermal Areas in Turkey and Preliminary Research on Biotechnologically Important Enzyme Production
dc.typeArticle
rdl.citation.openalex71
rdl.citation.scopus64
rdl.citation.wos46
rdl.oa.statusclosed
rdl.scopus.quartileQ2
rdl.source.indexSCOPUS
rdl.source.indexWOS
rdl.wos.indexScience Citation Index Expanded (SCI-EXPANDED)