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• MakaleAçık Erişim2026/r/gumushane/20432
  Thermostability and pH Differentiation in Geobacillus Hydrolases: A Comparative Perspective

  Akbiyik, E; Varol, A; Tekgul, ZB; Genc, B; Adiguzel, A

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• MakaleAçık Erişim2026/r/gumushane/13895
  Characterization and Bioactive Properties of Exopolysaccharides from Pediococcus ethanolidurans Isolated from Kırçan (Smilax excelsa L.) Pickles

  Karatas, Seyda Merve; Genc, Berna; Tunc, Merve Tugce

  In this study, the characterization of some bioactive content of exopolysaccharide (EPS) extracted from Pediococcus ethanolidurans isolated with Smilax excelsa L. from conventionally produced pickles were investigated. Background: Although this study is the main study involving the characterization of EPS obtained from Pediococcus ethanolidurans, it is important because it has determined a natural polysaccharide that can be used in different fields in the industry. According to the obtained results, sugar analysis by GC-MS revealed that EPS consisted of glucose (7.59%), mannose (41.96%), fructose (16.98%), arabinose (3.15%) and rhamnose (30.30%). From the thermal behavior determined by differential scanning calorimetry (DSC), it was concluded that it should not be heated close to 250 °C. At the same time, according to the thermogram obtained as a result of X-ray diffraction (XRD) analysis, it was found to have a crystalline structure. EPS, which reached 73.844% efficiency, showed antibacterial activity against Listeria monocytogenes, Staphylococcus aureus and Escherichia coli, while 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging activity was detected at 0.24 mM. It also has remarkable results, seen in cytotoxicity analysis against healthy HT-29 cells, demonstrating that it has proliferative activity as high as %125 In short, Pediococcus ethanolidurans was found to be a novel EPS producer with impressive properties.

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• MakaleAçık Erişim2026/r/gumushane/13734
  Preliminary Study on the Characterization of a Novel Polysaccharide from Enterococcus faecium SL60 Isolated from Siyez Sourdough; [Siyez Ekşi Mayasından İzole Edilen Enterococcus faecium SL60'tan Elde Edilen Yeni Bir Polisakkaritin Karakterizasyonu Üzerine Ön Çalışma]

  Genç, Berna

  This study reports the production, structural characterization, and preliminary cytotoxicity evaluation of an extracellular polysaccharide (EPS) synthesized by Enterococcus faecium SL60, a strain isolated from traditional Siyez (einkorn) sourdough. Analytical techniques including GC-MS, FTIR, XRD, zeta potential, and elemental analysis were applied to characterize the EPS. The purified EPS was found to be a water-soluble, negatively charged, amorphous heteropolysaccharide predominantly composed of mannose and glucose, with minor proportions of rhamnose, arabinose, galactose, and xylose. The total carbohydrate yield was 7.6 g/L. FTIR confirmed the presence of typical carbohydrate functional groups, while zeta potential analysis indicated a moderate surface charge (−9.71 mV), implying colloidal stability. Cytotoxicity evaluation using the CCD-1079Sk fibroblast cell line showed no significant toxicity at concentrations up to 500 µg/mL. These findings highlight the potential of SL60-derived EPS as a safe bioactive compound with possible applications in functional food development and health-related products.

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• MakaleAçık Erişim2024/r/gumushane/13971
  Bioethanol Production from Mulberry Molasses Waste with Ohmic-Assisted Hydrodistillation

  Tunç, Merve Tuğçe; Genç, Berna; Karataş, Şeyda Merve

  In this study, the fermentation process of bioethanol production conditions were optimized by response surface methodology. Mulberry molasses production waste was used as the only carbon source for yeast fermentation to produce bioethanol. Hydrodistillation (HD) and ohmic-assisted hydrodistillation (OAHD) methods were employed to concentrate the bioethanol. Fermentation time (48–168 h), waste matter rate (5–45%) and pH (5–7) were selected as independent variables. Alcohol concentration was treated as the response. Optimum fermentation conditions were determined as 96.894 h fermentation time, 45% waste ratio and pH 7. At these optimum conditions, alcohol concentration was determined as 3.77 ± 0.33%. While the distillate obtained in the HD method contained 22.50 ± 1.89% alcohol, it showed 27.72 ± 0.24% in the OAHD method. The energy consumption values for OAHD and HD was 2.92 ± 0.51 and 53.24 ± 1.74 Wh/mL bioethanol, respectively. Results of study showed that the OAHD could be a cost-effective and green method to distillation of bioethanol from mulberry molasses waste.

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• MakaleAçık Erişim2024/r/gumushane/12380
  Characterization of Water-Soluble Extracellular Polysaccharide from Aeribacillus pallidus IM17

  Genc, Berna; Tunç, Merve Tuğçe; Adiguzel, Ahmet

  The production of the extracellular polysaccharide from the thermophilic bacterium Aeribacillus pallidus was carried out in the study. The polysaccharide was isolated and characterized by means of GC–MS, FT-IR, DSC, and XRD analyses. The rheological, foaming, and emulsifying properties of the polysaccharide were determined. Using a sucrose-rich medium, 27.1 mg dried EPS/100 mL was obtained with 94% carbohydrate and 1.5% protein. The monosaccharide profile of water-soluble EPS-IM17 was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose. The foaming capacity and stability of EPS-IM17 were 26.67% (± 4.71) and 40.01% (± 4.95), respectively, and the foaming stability was not affected by time. The emulsification index of EPS-IM17 was 64.54 (± 8.71) and decreased to 38.47 (± 10.44) after 24 h. EPS-IM17 had a crystalline structure. Solutions at different concentrations (10, 20, 40 mgmL-1) showed pseudoplastic behavior. In conclusion, this report could be a lead study for the use of Aeribacillus pallidus extracellular polysaccharide for different applications.

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• MakaleAçık Erişim2024/r/gumushane/11751
  Enterococcus durans SL70, a Novel Exopolysaccharide Producer from Traditional Sourdough Fermentation of Einkorn (Triticum monococcum L. ssp. monococcum)

  Genc, Berna; Karatas, Seyda Merve; Tunç, Merve Tuğçe

  Research background. Given the potential of microbial exopolysaccharides from lactic acid bacteria in various industrial processes, alternative sources for the isolation of lactic acid bacteria are highly topical. In this study, we used a traditional sourdough from einkorn (Triticum monococcum L. ssp. monococcum) as a source of lactic acid bacteria for the isolation, identification and determination of exopolysaccharide producers. Experimental approach. The sourdough was prepared from einkorn according to the traditional method. Lactic acid bacteria were isolated and purified using the single colony technique on MRS and M17 media. The isolates were characterised using matrix-assisted laser desorption ionization-time of flight mass (MALDI-TOF) spectrometry. All isolates were analysed for extracellular polysaccharide production and one isolate was selected for purification and characterisation of its polysaccharide. Results and conclusions. The isolates were identified as Lactobacillus plantarum, L. paraplantarum, L. brevis, Pediococcus pentosaceus, Enterococcus faecium and E. durans. The production of exopolysaccharides by all lactic acid bacteria was evaluated and it was found that all strains (except one) were capable of producing exopolysaccharides. One polysaccharide (EPS-SL70) was purified from the isolates of E. durans SL70. This anionic heteropolysaccharide had, in addition to the carbohydrate backbone, a protein structure that did not contain nucleic acid. The carbohydrate backbone consisted of mannose, glucose, rhamnose, arabinose, xylose and galactose. Novelty and scientific contribution. The microbial flora of traditional einkorn sourdough has been identified in this study and represents the first report on the exopolysaccharide production by lactic acid bacteria in traditional einkorn sourdough. Additionally, Enterococcus durans from einkorn sourdough was identified as a new exopolysaccharide producer.

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• MakaleYalnız künye2021/r/gumushane/13299
  Exopolysaccharide of Anoxybacillus pushchinoensis G11 has antitumor and antibiofilm activities

  Genc, Berna; Taskin, Mesut; Adiguzel, Ahmet

  Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries. This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646). EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities. The viscosity of EPS showing Newtonian flow was temperature dependent. As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%). EPS contained 93% carbohydrates and 1.08% protein. The molecular weight of EPS was determined as 75.5 kDa. The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm−1), an indication of the antitumor effect. The EPS was semi-crystalline. It could maintain 36% of its weight at 800 °C and crystallization and melting temperatures were 221 and 255.6 °C. This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.

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• MakaleAçık Erişim2020/r/gumushane/14925
  Bacteriocin producing bacteria isolated from Turkish traditional sausage samples

  Erkaya, Elif; Genc, Berna; Akbulut, Sumeyya; Adiguzel, Gulsah; Omeroglu, Mehmet Akif; Ozkan, Hakan; Adiguzel, Ahmet

  In this study, traditional sausage samples from different provinces of Turkey (Gaziantep, Antalya, erzurum and Kahramanmaras) were obtained and one hundred three isolates were collected. Using the (GTG)5-PCR genomic fingerprint analysis method, seven of them were observed to be different and conventional tests of these isolates were performed. Molecular identification of two isolates carrying the bacteriocin gene and having antimicrobial activity by agar disc diffusion method was performed by 16S rRNA sequence analysis. As a result, the seven isolates were identified as Aerococcus urinaeequi (EK1), Streptococcus salivarius (EK2), Leuconostoc mesenteroides (EK3), Macrococcus caseolyticus (EK4), Lactococcus garvieae (EK5), Staphylococcus saprophyticus (EK6) and Lactobacillus sakei (EK7). Among these strains, it has been determined that Ln. mesenteroides and L. sakei carried the mecentericin and sacacin genes. When antimicrobial activity against different strains was examined, inhibition formations of Ln. mesenteroides and L. sakei on Enterococcus faecalis, Shigella dysenteriae and Escherichia coli O157: H7 were observed.

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• MakaleAçık Erişim2020/r/gumushane/13331
  Proteolytic, lipolytic and amylolytic bacteria reservoir of Turkey; cold-adaptive bacteria in detergent industry

  Muslu, Selman; Genc, Berna; Adiguzel, Mahmut; Albayrak, Seyda; Adiguzel, Ahmet

  Enzymes which would be active in cold conditions can be used in a wide range of fields from molecular biology to detergent industry due to their low processing capacity and high activity. In this study, sixty cold-adapted bacteria were isolated from water and sludge samples collected from Erzurum and Van provinces. Identification of eight isolates by molecular [(GTG)5-PCR and 16S rRNA sequencing] techniques and tests for temperature (4-35°C), pH (3-11) and salt (2-15% (w/v) requirements were performed. These bacteria were belonging to Pseudomonas chlororaphis subsp. aureofaciens (SM011A), Psychrobacter faecalis (SM012D), Rahnella aquatilis (SM015A), Shewanella putrefaciens (SM018A), Pseudomonas lactis (SM0110A), Flavobacterium chryseum (SM0112E), Exiguobacterium mexicanum (SM0117A) and Glutamicibacter arilaitensis (SM0118A). The physicochemical requirements for all isolates ranged between 4-25°C, pH 5-7 and 2-15% salt (NaCl) concentration. However, E. mexicanum did not require salt in growth medium. All bacteria were evaluated for protease, lipase and amylase enzymes and all were found to be multiple enzyme producers. The eight isolates were identified from the resources of Turkey, for the first time and enzyme production abilities of some isolates to produce enzymes were declared. The originating of the producers of these enzymes from Turkey shows that Turkey has a remarkable reservoir for cold-adaptive microorganisms and these microorganisms will make important contributions to the detergent industry worldwide.

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• MakaleYalnız künye2019/r/gumushane/15296
  A novel endo-β-1,4-xylanase from Pediococcus acidilactici GC25; purification, characterization and application in clarification of fruit juices

  Adiguzel, Gulsah; Faiz, Ozlem; Sisecioglu, Melda; Sari, Bilge; Baltaci, Ozkan; Akbulut, Sumeyya; Genc, Berna; Adiguzel, Ahmet

  A novel extracellular xylanase was purified and characterized from Pediococcus acidilactici GC25 (GenBank number: MF289522). The purification was 4.6-fold with a yield of 43.61% through acetone precipitation, Q-Sepharose, and CM-Sepharose ion change chromatography. The molecular weight of the enzyme was 48.15 kDa, and the optimum pH and temperature were 7.0 and 40 °C, respectively. The maximum activity was observed between 20 and 50 °C. Although it was active within a wide pH range (pH 2.0–9.0), it retained over 85% of its activity after 24 h incubation; and over 70% of its activity after 168 h incubation in neutral and alkaline pH. It was observed that the enzyme showed high stability with K+, Ba2+, Cd2+, Co2+, Sr2+, Mg2+, Ca2+, Al3+, Zn2+, and Ni2+ ions. The Km and Vmax for the xylanase were 3.10 mg mL−1 and 4.66 U/mg protein, respectively. It was determined that treatment of different fruit juices with P. acidilactici GC25 xylanase improved the clarification. The highest increase in the reducing sugar amount and decrease in the turbidity was 24.47 ± 1.08 and 21.22 ± 0.58 for peach juice at 0.15 U/mL enzyme concentration. These results showed that the xylanase purified from P. acidilactici GC25 may have a wide potential in biotechnological processes of the food and baking industry.

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