Gümüşhane ÜniversitesiGümüşhane ÜniversitesiAçık Erişim Arşivi · RDL Open

The effect of homocysteine on the expression of CD36, PPARγ, and C/EBPα in adipose tissue of normal and obese mice

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  • SDG 3 · Sağlık ve Kaliteli Yaşam

Özet

The purpose of this study was to investigate the effect of homocysteine (Hcy) on CD36, PPARγ, and C/EBPα gene and protein expression in adipose tissue obtained from normal and high-calorie diet obesity models. CD36, PPARγ, and C/EBPα gene expression and protein levels in adipose tissue specimens were determined using the RT-PCR and ELISA methods, respectively. Significantly increased CD36 gene expression was observed in adipose tissue from obese mice, while Hcy significantly reduced CD36 gene expression in adipose tissue from normal and obese mice. PPARγ and C/EBPα gene expression levels decreased significantly in all groups compared to the normal group. In addition, levels of both PPARγ and C/EBPα gene expression were lower with Hcy supplementation compared to their own controls. In conclusion, Hcy’s reduction of CD36 gene expression in adipose tissue may be one probable factor in hyperhomocysteinemia representing an independent risk factor for cardiovascular diseases.

Anahtar Kelimeler

Adipose tissueC/EBPαcardiovascular diseasesCD36hyperhomocysteinemiaobesityPPARγ
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Tam Kayıt
AlanDeğerDil
dc.contributor.authorMentese, Ahmet
dc.contributor.authorDogramaci, Seniz
dc.contributor.authorDemir, Selim
dc.contributor.authorYaman, Serap Ozer
dc.contributor.authorInce, Imran
dc.contributor.authorAltay, Diler Us
dc.contributor.authorErdem, Mehmet
dc.contributor.authorTuran, Ibrahim
dc.contributor.authorAlver, Ahmet
dc.date.accessioned2026-10-22T10:23:11.660577167Z
dc.date.available2026-10-22T10:23:11.660577167Z
dc.date.issued2021
dc.description.abstractThe purpose of this study was to investigate the effect of homocysteine (Hcy) on CD36, PPARγ, and C/EBPα gene and protein expression in adipose tissue obtained from normal and high-calorie diet obesity models. CD36, PPARγ, and C/EBPα gene expression and protein levels in adipose tissue specimens were determined using the RT-PCR and ELISA methods, respectively. Significantly increased CD36 gene expression was observed in adipose tissue from obese mice, while Hcy significantly reduced CD36 gene expression in adipose tissue from normal and obese mice. PPARγ and C/EBPα gene expression levels decreased significantly in all groups compared to the normal group. In addition, levels of both PPARγ and C/EBPα gene expression were lower with Hcy supplementation compared to their own controls. In conclusion, Hcy’s reduction of CD36 gene expression in adipose tissue may be one probable factor in hyperhomocysteinemia representing an independent risk factor for cardiovascular diseases.
dc.description.sponsorshipKaradeniz Technical University Scientific Research Foundation, (5323)
dc.identifier.doi10.1080/13813455.2019.1648517
dc.identifier.eissn1744-4160
dc.identifier.endpage444
dc.identifier.issn1381-3455
dc.identifier.issue5
dc.identifier.pmid31373231
dc.identifier.scopus2-s2.0-85116166089
dc.identifier.startpage437
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/gumushane/14823
dc.identifier.volume127
dc.identifier.wosWOS:000480150000001
dc.language.isoen
dc.publisherTaylor and Francis Ltd.
dc.relation.ispartofArchives of Physiology and Biochemistry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAdipose tissue
dc.subjectC/EBPα
dc.subjectcardiovascular diseases
dc.subjectCD36
dc.subjecthyperhomocysteinemia
dc.subjectobesity
dc.subjectPPARγ
dc.subject.classificationBiochemistry & Molecular Biology
dc.subject.classificationBiophysics
dc.subject.classificationEndocrinology & Metabolism
dc.subject.classificationPhysiology
dc.subject.otherAdipose Tissue
dc.subject.otherAnimals
dc.subject.otherCCAAT-Enhancer-Binding Protein-alpha
dc.subject.otherCD36 Antigens
dc.subject.otherHomocysteine
dc.subject.otherMice
dc.subject.otherObesity
dc.subject.otherPPAR gamma
dc.subject.otherCCAAT enhancer binding protein alpha
dc.subject.otherCD36 antigen
dc.subject.otherperoxisome proliferator activated receptor gamma
dc.subject.otheranimal
dc.subject.othermouse
dc.titleThe effect of homocysteine on the expression of CD36, PPARγ, and C/EBPα in adipose tissue of normal and obese mice
dc.typeArticle
dspace.entity.typePublication
rdl.citation.openalex2
rdl.citation.scopus2
rdl.citation.wos2
rdl.departmentDepartment of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences, Gumushane University, Gumushane, Turkey
rdl.oa.statusclosed
rdl.scopus.quartileQ2
rdl.sdgSDG03
rdl.source.indexSCOPUS
rdl.source.indexWOS
rdl.source.indexPUBMED
rdl.wos.indexScience Citation Index Expanded (SCI-EXPANDED)