Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2026/r/gumushane/13669
  Phenolic Profiling of Laurus nobilis by LC–ESI–MS/MS: In Vitro Bioactivities and In Silico Enzyme Docking

  Bak, Funda Erşen; Canbolat Gültekin, Deniz; Akmeşe, Osman; Atalay, Abdurrahman; Turan, İbrahim; Gültekin, Ergün; Demir, Selim; Aliyazicioglu, Yüksel

  This study evaluated the branch, leaf, and fruit extracts of Laurus nobilis collected from Artvin in terms of phenolic composition, antioxidant, antimicrobial, and antidiabetic activities and additionally investigated the binding of selected phenolics to α-amylase/α-glucosidase targets through molecular docking. Using LC–ESI–MS/MS analysis, 31 phenolic compounds were quantitatively identified, with epicatechin, luteolin-7-O-glucoside, and rutin predominating particularly in the branches and leaves. Antioxidant capacity was higher in branches and leaves (total phenolic content [TPC]/FRAP/DPPH IC50: branches and leaves, respectively, 95.27–60.84 mg gallic acid equivalent [GAE]/g, 206.16–162.05 mg Trolox equivalent [TE]/g, and 46.95–113.49 µg/mL). Antimicrobial activity was observed within a minimum inhibitory concentration (MIC) range of 1.25–10 mg/mL; leaves exhibited the strongest effect against Bacillus cereus (1.25 mg/mL), whereas fruits were most effective against Candida albicans (1.25 mg/mL). In antidiabetic evaluation, fruits yielded the lowest IC50 values against α-amylase and α-glucosidase (4.31 and 8.97 mg/mL, respectively), suggesting a “composition-over-quantity” effect despite low total phenolic content. Docking results supported high binding affinities of rutin (α-glucosidase −9.3 kcal/mol; α-amylase −8.1 kcal/mol) and luteolin (−8.6 kcal/mol for both enzymes). Overall, although branches and leaves exhibited strong antioxidant profiles, fruits showed more pronounced inhibitory effects on digestive enzymes; therefore, fractionation studies and in vivo/kinetic validations are recommended.

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• MakaleYalnız künye2026/r/gumushane/13421
  LC–MS/MS Phenolic Profiling and Antioxidant, Antimicrobial, and Antidiabetic Activities of Caucasian Propolis

  Gültekin, Deniz Canbolat; Turan, İbrahim; Akmeşe, Osman; Gültekin, Ergün; Demir, Selim; Aliyazicioglu, Yüksel

  Propolis is a natural bee product rich in phenolic and flavonoid compounds and is well known for its remarkable biological properties. In this study, the phenolic compositions of propolis samples collected from various localities in the Caucasus region of Türkiye were characterized using LC–MS/MS, and their antioxidant, antidiabetic, and antibacterial activities were also evaluated. LC–MS/MS analyses identified caffeic acid, ferulic acid, and p-coumaric acid as the major phenolic acid compounds, while pinocembrin, chrysin, and galangin were determined as the major flavonoid constituents. Overall, these findings indicate that the antioxidant capacity of propolis samples obtained from different regions may be particularly associated with these key phenolic and flavonoid compounds. Consistent with this composition, total phenolic and flavonoid contents ranged from 34.72 to 120.54 mg GAE/g and 6.88 to 47.19 mg QE/g, respectively. The extracts exhibited a relatively high antioxidant capacity, with FRAP values between 60.47 and 311.87 mg TE/g and DPPH IC50 values ranging from 215.80 to 37.96 µg/mL. Regarding antidiabetic activity, the IC50 values for α-amylase and α-glucosidase inhibition were determined as 5.86–3.47 and 12.22–5.02 mg/mL, respectively, indicating a pronounced antidiabetic potential. Antimicrobial analyses revealed activity against E. faecalis, B. cereus, E. coli, K. pneumoniae, and C. albicans. Overall, this study provides a comprehensive LC–MS/MS-based phenolic profile of Caucasian propolis and demonstrates its antioxidant properties together with its antidiabetic potential.

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• MakaleAçık Erişim2023/r/gumushane/10317
  An Investigation of the Protective Effect of Rhododendron luteum Extract on Cisplatin-Induced DNA Damage and Nephrotoxicity and Biochemical Parameters in Rats

  Turan, Ibrahim; Canbolat, Deniz; Demir, Selim; Kerimoglu, Gokcen; Colak, Fatih; Alemdar, Nihal Turkmen; Mentese, Ahmet; Aliyazicioglu, Yuksel

  The nephrotoxicity mechanisms caused by cisplatin (CP), which is used in the treatment of different types of cancer, include several processes, such as DNA damage, apoptosis, inflammation, and oxidative stress. This study investigated the protective effects of Rhododendron luteum leaf extract (RLE) in a rat model of CP -induced nephrotoxicity. Dimethyl sulfoxide extract of RLE was prepared, and its contents were clarified using liquid chromatography-mass spectrometry (LC-MS -MS). Thirty female Wistar Albino rats were divided into five different groups and sacrificed on the seventh day. Biochemical and histopathological analyses were then performed. The presence of quercitrin, quinic acid, chlorogenic acid, protocatechic acid, isoquercitrin, and quercetin was detected in the RLE. While CP significantly increased the levels of oxidative stress, inflammation, and apoptosis markers in the rat kidney tissue and blood samples, treatment with RLE significantly reversed the levels of these parameters in a dose-dependent manner. Total antioxidant status (TAS) values and glutathione (GSH) levels increased significantly in the serum samples from the treatment group compared to the CP group, while blood urea nitrogen (BUN), creatinine, oxidative stress index (OSI), tumor necrosis factor-alpha (TNF-alpha), malondialdehyde (MDA) and total oxidant status (TOS) values decreased significantly. GSH levels increased significantly in the treatment group compared to the CP group, while TNF-alpha, caspase-3 (Casp-3), 8-hydroxy-2'-deoxyguanosine (8-OHdG), OSI, TOS, MDA levels, and damage scores decreased significantly at histopathological examination. In conclusion, RLE exhibited strong protective effects against nephrotoxicity and oxidative damage caused by CP in rats.

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• MakaleYalnız künye2022/r/gumushane/13048
  An Investigation of the Antiproliferative Effect of Rhododendron luteum Extract on Cervical Cancer (HeLa) Cells via Nrf2 Signaling Pathway

  Turan, Ibrahim; Demir, Selim; Yaman, Serap Ozer; Canbolat, Deniz; Mentese, Ahmet; Aliyazicioglu, Yuksel

  The aim of the present study was to investigate the role of Rhododendron luteum extract (RLE) in the induction of Nrf2‑related oxidative stress and endoplasmic reticulum (ER) stress in human cervical cancer (HeLa) cells. The antiproliferative effect of RLE on HeLa and fibroblast cells was determined using the MTT assay. The effects of RLE on the cell cycle, apoptosis, and production of reactive oxygen species (ROS) in HeLa cells were evaluated using fluorescent probes. The mRNA expression levels of Nrf2 [and its targets glutamate-cysteine ligase catalytic subunit (GCLC), and glucose-6-phosphate dehydrogenase (G6PD)], and C/EBP homologous protein (CHOP, an ER stress marker were determined using reverse transcription‑quantitative polymerase chain reaction (RT-PCR). The results demonstrated that RLE exhibited a selective cytotoxic effect (2.9-fold) on HeLa cells compared to fibroblast cells. RLE arrested the cell cycle at the S phase, and induced apoptosis, ER stress, and ROS formation. In addition, RLE significantly suppressed the expression levels of Nrf2, GCLC and G6PD (0.65, 0.69, and 0.54-fold, respectively) and increased the expression of CHOP (4.48-fold) in HeLa cells at 72 h of treatment (p < 0.05). These results show that the antiproliferative effect of RLE occurs through the Nrf2 and ER stress pathways, and the results should now be supported by further in vivo studies.

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• MakaleAçık Erişim2021/r/gumushane/16314
  The Effect of Rosa pimpinellifolia Extract on the Proliferation of Human Tumor Cells

  Demir, Elif Ayazoglu; Demir, Selim; Türkmen, Nihal; Turan, Ibrahim

  The aim of this study was to evaluate the antioxidant properties of R. pimpinellifolia and its cytotoxic effect against human colon (WiDr), liver (HepG2) and lung (A549) cancer cells compared with a normal fibroblast (BJ) cell line. The antioxidant properties of extract was determined using spectrophotometric methods, while the cytotoxic effect of extract was evaluated using MTT assay. The total phenolic content, total flavonoid content and reducing power values of extract were calculated as 16.4 +/- 0.4 mg gallic acid equivalent, 5.2 +/- 0.2 mg quercetin equivalent and 34.3 +/- 2.4 mg trolox equivalent per g sample, respectively. Although the extract exhibits selective cytotoxic effect on three cancer cell lines in a dose-dependent manner compared to BJ cells, the highest selectivity index value (2.7-fold) was obtained for the WiDr cell line. In conclusion, this is the first study to demonstrate the cytotoxic effect of R. pimpinellifolia on cancer cells. To demonstrate the molecular mechanism of this cytotoxic effect and to carry out in vivo studies may be a guide for future clinical studies.

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• MakaleAçık Erişim2021/r/gumushane/16277
  Evaluation of Cytotoxic Effect of Onosma armeniacum Extract on Various Cancer Cells

  Demir, Selim; Ayazoglu Demir, Elif; Turan, Ibrahim; Özgen, Ufuk

  The aim of this study was to investigate the antioxidant properties of the root extract of Onosma armeniacum and its cytotoxic effect against human liver (HepG2), lung (A549) and colon (WiDr) cancer cell lines compared with a normal fibroblast cell line. Antioxidant properties of the extract was determined using the colorimetric methods. The cytotoxic effect of the extract were investigated using MTT assay. The total phenolic content and reducing power values of extract were 26.1 +/- 0.4 mg gallic acid equivalent and 52.6 +/- 1.1 mg trolox equivalent per g sample, respectively. The extract exhibited selective cytotoxic effect in all studied cancer cell lines, especially HepG2 (3.6-fold). The results of this study reveal that O. armeniacum, which has been found to have acceptable antioxidant and cytotoxic effects, should be examined with more comprehensive studies.

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• MakaleAçık Erişim2021/r/gumushane/15861
  Investigation of the Cytotoxic Effect of Ethyl Pyruvate on Various Cancer Cell Lines

  Ayazoglu Demir, Elif; Demir, Selim; Turan, Ibrahim

  Ethyl pyruvate (EP) is a simple aliphatic ester derived from pyruvic acid which is an endogenous metabolite. Although various studies have investigated the antioxidant and anti-inflammatory properties of EP, there has been only limited research into the cytotoxic effect of EP on cancer cells. The aim of this study was to determine the cytotoxic effects of EP on cells representing common cancer types. EP was purchased commercially and intermediate stock solutions were prepared with phosphate buffer saline. The cytotoxic effect of EP on human melanoma (VMM917), cervix (HeLa), breast (MCF-7), lung (A549), liver (HepG2), colon (WiDr) cancer and normal fibroblast (BJ) cells was determined using the MTT assay. Cisplatin was used as a positive control in cytotoxicity experiments. The results showed that EP exhibits selective cytotoxic effect on VMM917 (10.1-fold) and HeLa (3.04-fold) cells compared to BJ cells. This study shows for the first time that EP has a highly selective cytotoxic effect, especially on melanoma and cervix cancer cells. The mechanism of this effect needs to be elucidated by more extensive studies.

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• MakaleYalnız künye2021/r/gumushane/14823
  The effect of homocysteine on the expression of CD36, PPARγ, and C/EBPα in adipose tissue of normal and obese mice

  Mentese, Ahmet; Dogramaci, Seniz; Demir, Selim; Yaman, Serap Ozer; Ince, Imran; Altay, Diler Us; Erdem, Mehmet; Turan, Ibrahim; Alver, Ahmet

  The purpose of this study was to investigate the effect of homocysteine (Hcy) on CD36, PPARγ, and C/EBPα gene and protein expression in adipose tissue obtained from normal and high-calorie diet obesity models. CD36, PPARγ, and C/EBPα gene expression and protein levels in adipose tissue specimens were determined using the RT-PCR and ELISA methods, respectively. Significantly increased CD36 gene expression was observed in adipose tissue from obese mice, while Hcy significantly reduced CD36 gene expression in adipose tissue from normal and obese mice. PPARγ and C/EBPα gene expression levels decreased significantly in all groups compared to the normal group. In addition, levels of both PPARγ and C/EBPα gene expression were lower with Hcy supplementation compared to their own controls. In conclusion, Hcy’s reduction of CD36 gene expression in adipose tissue may be one probable factor in hyperhomocysteinemia representing an independent risk factor for cardiovascular diseases.

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• MakaleYalnız künye2020/r/gumushane/14974
  Effect of Turkish Propolis on miRNA Expression, Cell Cycle, and Apoptosis in Human Breast Cancer (MCF-7) Cells

  Misir, Sema; Aliyazicioglu, Yüksel; Demir, Selim; Turan, Ibrahim; Hepokur, Ceylan

  Enriched in flavonoid compounds, phenol acids, and terpene derivatives, propolis has been shown to regulate apoptosis signaling pathways and alter the expression of microRNAs (miRNAs). In the present study, it has been aimed to examine the effects of Turkish propolis on miRNA levels of breast cancer (MCF-7) cells, and its relationship with cell proliferation and apoptosis. Cytotoxic activity of ethanolic propolis extract (EEP) was evaluated using MTT assay. Mechanisms involved in the cytotoxic action of Turkish propolis in MCF-7 cells were investigated with regard to apoptosis and cell cycle using flow cytometry and western blot. Mitochondrial membrane potential (MMP) were evaluated by spectrofluorometric method. miRNA levels were detected by qRT-PCR method. EEP exhibited selective toxicity against MCF-7 cells compared to normal fibroblast cells. EEP increased the cell cycle arrest at the G1 phase. EEP elevated the apoptotic cell death through increasing pro-apoptotic protein levels (p21, Bax, p53, p53-Ser46, and p53-Ser15), decreasing MMP and altering the expression levels of specific tumor suppressors (miR-34, miR-15a, and miR-16-5p) and oncogenic (miR-21) miRNAs. These data support that Turkish propolis may be evaluated as a potential natural agent for new anticancer drugs in future.

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• MakaleYalnız künye2020/r/gumushane/13240
  Rosa canina Extract has Antiproliferative and Proapoptotic Effects on Human Lung and Prostate Cancer Cells

  Kilinc, Kagan; Demir, Selim; Turan, Ibrahim; Mentese, Ahmet; Orem, Asim; Sonmez, Mehmet; Aliyazicioglu, Yuksel

  Although several studies have investigated the cytotoxic effects of different Rosa species, there has been only limited research into the cytotoxic effect of Rosa canina. The purpose of this research was to evaluate the antioxidant properties, phenolic characterization, and cytotoxic effects of R. canina on human lung (A549) and prostate (PC-3) cancer cells and the possible mechanisms involved. The antioxidant properties and phenolic characterization of the extract were determined using spectrophotometric methods and RP-HPLC, respectively. The cytotoxic activity of the extract was determined using the MTT assay. The mechanism involved in the extract’s cytotoxic effect was then evaluated in terms of apoptosis, the cell cycle, mitochondrial membrane potential (MMP), and caspase activity using fluorometric and luminometric methods. The TPC value of the extract was 58.97 ± 2.22 mg gallic acid equivalents per gram sample, and ascorbic acid and p-coumaric acid were detected as major phenolics in the extract. R. canina extract exhibited a selective cytotoxic effect on A549 and PC-3 cells compared to normal fibroblast cells. The extract induced cell cycle arrest at the G1 phase and apoptosis via reduced MMP and increased caspase activity in these cells. Phytomedical applications of R. canina may represent promising approaches in the treatment of cancer.

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