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- MakaleAçık Erişim2025Effects of Varying Ratios of Hawthorn Fruit Extract on Nesfatin-1, GLP-1 and cTnI Response in Streptozotocin-induced Diabetic Rats
Özcan, G. Böyük; Kaya, H.
Background: The aim of this study was to investigate the ef fects of Hawthorn (Crataegus oxyacantha) Fruit Extract on the response of nesfatin-1, Glucagon-like peptide 1 (GLP-1) and cardiac troponin I (cTnI) in STZ-induced diabetic rats. Methods: This study utilized 64 adult male Wistar albino rats, aged 8-10 weeks and weighing an average of 195-215 g. Eight groups were randomly selected, each comprising eight rats designated as Control (C), H100, H200, H300; Diabetes Control (DC), DH100, DH200, DH300. Blood samples were collected from the tail vein (vena caudalis) of all individuals on days 0 and 21 of the research. The serum levels of Nesfatin-1, GLP-1 and cTnI were quantified and assessed using the ELISA technique. Result: In diabetic groups, the increase in serum mean nesfatin-1 and cTnI levels was most pronounced in DC groups, while the most significant decrease due to increased HFE supplementation was observed in DR200 groups (p<0.01); whereas in diabetic groups, the decrease in serum mean GLP-1 levels was observed in DC group, while the most significant increase due to increased HFE supplementation was detected in DG200 group. As a result, it was concluded that 200 mg/kg RPE administration to healthy and di abetic rats di d not cause any side eff ects. It was concluded that HFE, which has an anti-diabetic eff ect by regulating the inflammatory response that plays a role in the development of diabetes, decreasing nesfatin-1 levels and increasing GLP-1 levels, may be useful in supporting the impaired pancreatic beta cell function that occurs in diabetes.
- MakaleAçık Erişim2016Increased expressions of eNOS and iNOS correlate with apoptosis of diabetic nephropathy in streptozotocin-induced type 1 diabetic rats; [Streptozotosin kaynaklı tip 1 diyabetik sıçanlarda meydana gelen nefropatilerde artan eNOS ve iNOS sunumlarının apoptozisle ilişkilendirilmesi]
Dinçel, Güngör Çağdaş; Yildirim, Serkan
The present study was designed to evaluate the effects of high level of nitric oxide (NO) production and oxidative stress (OS) on nephropathology and to identify whether NO and OS have any correlation with apoptosis seen in diabetic kidney, elucidating the underlying mechanism(s) involved in the development of nephropathology in streptozotocin (STZ)-induced diabetic rats. Expression levels of caspase 3, caspase 9, endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), 8-hydroxy-2′-deoxyguanosine (8-OHdG), for the detection of oxidative damage to DNA, were examined in diabetic kidney tissues. Results of the study revealed that the levels of 8-OHdG (P<0.005), eNOS (P<0.005), iNOS (P<0.005), caspase 3 (P<0.005) and caspase 9 (P<0.005) were remarkably higher in diabetic kidney tissues than in controls. In addition, STZ-treated animals showed significant loss of body weight and renal enlargement. It was suggested that apoptosis, OS and increased NO levels are involved in the pathogenesis of diabetic nephropathy. The results also strongly suggested that STZ-induced apoptosis through activation of the intrinsic pathway and that might be most likely related to increased NO levels. Moreover, high NO production was not only mediated by eNOS but also by iNOS. Increased NO production may contribute to hyperfiltration and microalbuminuria in early diabetic nephropathy. Furthermore, expression of 8-OHdG might give an idea of the progress and may be essential as it has a diagnostic significance for this disease. In conclusion, we believe that eNOS and iNOS overexpressions induce diabetic nephropathy by mediating apoptosis in STZ-induced rats.