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- MakaleAçık Erişim2019Antioxidant Properties of Primula vulgaris Flower Extract and Its Cytotoxic Effect on Human Cancer Cell Lines
Demir, Selim; Turan, Ibrahim; Aliyazicioglu, Yuksel
Primula vulgaris is a plant belonging to Primula genus, and these species are often used in traditional therapy. Although there are numerous studies on the antioxidant and cytotoxic effects of different Primula species, there are limited studies of these properties of P. vulgaris extracts. The aim of this study was to determine the antioxidant properties and cytotoxic effects of the extract prepared with dimethyl sulfoxide from P. vulgaris flowers. The total phenolic content, total flavonoid content, and reducing antioxidant power of the extract were determined using spectrophotometric methods. The cytotoxic effect of the extract on human colon (WiDr), lung (A549), liver (HepG2), breast (MCF-7), and prostate (PC-3) cancer cells and a normal human fibroblast cell line was assessed using the MTT assay. Total phenolic content and reducing power values of extract were found 33.02 +/- 0.92 mg gallic acid equivalents, and 64.86 +/- 1.18 mg trolox equivalents per g sample, respectively. Extract exhibited selective cytotoxic effect on all studied cancer cells compared to normal fibroblast cells, and the IC50 values of the extract in the cancer cell lines range from 191.8 to 375.3 mu g/mL. This work is one of the pioneering work that reveals the powerful antioxidant properties of P. vulgaris flower extract and its selective cytotoxic effect on cancer cell lines. Further studies are needed to identify the active molecules in the extract and the cytotoxic action mechanisms of these molecules.
AtıfWoS20OpenAlex22TR Dizin9
İncele - MakaleAçık Erişim2018Scopus SJRQ1Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells
Demir, Selim; Turan, Ibrahim; Aliyazicioglu, Rezzan; Yaman, Serap Ozer; Aliyazicioglu, Yuksel
Primula vulgaris belongs to the genus Primula, members of which are frequently used in folk medicine. Various studies have investigated the cytotoxic effect of different Primula species, but there have been limited studies on the cytotoxic effect of P. vulgaris. The aim of this study was to investigate the cytotoxic effects, and possible mechanisms involved, of P. vulgaris flower extract on human cervical cancer (HeLa) cells. The cytotoxic effect of the extract on HeLa cells was revealed using the MTT assay. Mechanisms involved in the extract's cytotoxic effect were then investigated in terms of apoptosis, mitochondrial membrane potential, and the cell cycle, using fluorometric methods. P. vulgaris flower extract exhibited selective cytotoxic effects against HeLa cells by arresting their cell cycle at the S phase, and inducing the number of apoptotic cells compared to normal fibroblast cells by reducing mitochondrial membrane potential in a concentration-dependent manner. This is the first study to reveal the antiproliferative effect of P. vulgaris flower extract. Further studies are now needed to identify the cytotoxic molecules in the extract and their mechanisms.
AtıfScopus29WoS32OpenAlex40
İncele - MakaleAçık Erişim2017Evaluation of Antioxidant and Cytotoxic Properties of Primula vulgaris Leaf Extract
Turan, Ibrahim; Demir, Selim; Aliyazicioglu, Rezzan; Aliyazicioglu, Yuksel
Primula vulgaris belongs to the genus Primula and this genus is frequently used in traditional medicine. Numerous studies reported the antioxidant and cytotoxic effects of various extracts of different Primula species but, there were limited studies of the antioxidant and cytotoxic effect of P. vulgaris. The aim of this study was to determine the antioxidant properties and cytotoxic effects of dimethyl sulfoxide extract of leaves of P. vulgaris. The total polyphenolic content (TPC), total flavonoid content (TFC) and reducing antioxidant power of extract were evaluated using spectrophotometric procedures. The cytotoxic effects of extract were revealed on human lung (A549), liver (HepG2), breast (MCF-7), prostate (PC-3), and colon (WiDr) cancer cell lines and normal fibroblast cells using thiazolyl blue tetrazolium bromide (MTT) assay. TPC and FRAP values were 1890.1 +/- 11.2 mg gallic acid equivalents and 3233.1 +/- 176.2 mg trolox equivalents per 100 g sample, respectively. The extract showed selective cytotoxic effects on all studied cancer cell lines compared to normal fibroblast cells and IC50 values ranged from 133.3-253.8 mu g/mL. This is the first study to reveal the antioxidant properties of P. vulgaris leaf extract and its cytotoxic effect on cancer cell lines. Further studies are now needed to identify the antioxidant and cytotoxic molecules in the extract and their mechanisms.
AtıfWoS15OpenAlex6İncele - MakaleAçık Erişim2017Scopus SJRQ2Phenolic characterisation and antioxidant activity of Primula vulgaris and its antigenotoxic effect on fibroblast cells
Ozkan, Mehtap Tugce; Aliyazicioglu, Rezzan; Demir, Selim; Misir, Sema; Turan, Ibrahim; Yildirmis, Sermet; Aliyazicioglu, Yuksel
Background: Primula vulgaris has been used in traditional treatment, and its biological functions are attributed to its polyphenolics content. Objectives: The aim of this study was to determine the phenolics composition and the antioxidant activity of water extract of P. vulgaris (WEP) and to determine its probable preventive effects against H2O2-induced DNA damage in human fibroblast cells. Methods: The total polyphenolic content (TPC), ferric reducing antioxidant power (FRAP), and radical scavenging activity of WEP were determined using spectrophotometric methods. Phenolic compounds and antigenotoxic effects of WEP were evaluated using HPLC and comet assay, respectively. Results: The TPC and FRAP values of WEP were 15.023 ± 0.84 mg gallic acid and 82.63 ± 0.31 μM trolox per g sample, respectively. ρ-coumaric acid and rutin were detected as major phenolics. Moreover, WEP reducedH2O2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to the positive controls (only 20 μMH2O2 treatment). Conclusions: Primula vulgaris can be used in food, cosmetics, and drug industries because of its antioxidant and antigenotoxic activities.
AtıfScopus26WoS30OpenAlex25İncele