Sürdürülebilir Kalkınma Amaçları

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Yazarlar

• Balci, Mecdi Gurhan(1)
• Cagatay, Diren Vuslat(1)
• Das, Huseyin(1)
• Demir, Fatih(1)
• Demir, Selim(1)
• Issin, Gizem(1)
• Turan, Ibrahim(1)

• MakaleAçık Erişim2025Scopus SJRQ2/r/gumushane/16369
  EZH2 expression in colorectal carcinoma: an evaluation of clinicopathological and prognostic value

  Cagatay, Diren Vuslat; Balci, Mecdi Gurhan; Issin, Gizem; Demir, Fatih

  BackgroundEnhancer of zeste homolog 2 (EZH2), the catalytic subunit of Polycomb Repressive Complex 2, catalyzes trimethylation of histone H3 lysine 27 and has been implicated in tumor progression.AimOur study aims to assess the relationship between EZH2 expression and clinicopathologic data, and survival in colorectal carcinomas (CRC).Materials and methodsEZH2 immunohistochemistry was performed on tumor blocks from 124 CRC patients. Based on H-scores, cases were stratified into low- and high-expression groups. EZH2 status was correlated with demographic, clinical, and pathologic features, and overall survival was analyzed with the Kaplan-Meier method and log-rank test.ResultsA total of 124 cases of CRC; 12 (9.7%) cases were classified as low EZH2 expression, and 112 (90.3%) cases were classified as high EZH2 expression. A significant association was found between EZH2 expression and microsatellite stability. High EZH2 expression was significantly enriched in microsatellite-stable tumors (p = 0.007) and in left-sided lesions (p = 0.049). No significant associations were detected with sex, stage, grade, lymph-node status, or vascular invasion. Kaplan-Meier analysis revealed no difference in overall survival between low- and high-EZH2 groups (log-rank p = 0.47; hazard ratio = 1.13, 95% CI 0.45-2.85). EZH2 staining was uniformly strong in normal mucosa and adenomas, mirroring the high expression observed in most CRCs.ConclusionsEZH2 is highly expressed across the normal-adenoma-carcinoma sequence and, in our cohort, was not linked to overall survival or to most clinicopathologic parameters in CRC. The lower expression observed in a subset of MSI-H tumors warrants further investigation; present evidence remains insufficient to establish EZH2 as an independent prognostic biomarker.

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  İncele
• MakaleYalnız künye2015Scopus SJRQ4/r/gumushane/15501
  Genetic characterization of the Akkaraman, Morkaraman and Karayaka sheep through microsatellite variation

  Das, Huseyin; Turan, Ibrahim; Demir, Selim

  Genotypes have to be controlled periodically and necessary precautions should be taken in order to ensure that animal biodiversity is not lost. In this study, the genotypes of the Akkaraman, Karayaka and Morkaraman species from among Turkey’s local sheep were examined at 7 different microsatellite loci. The average allele counts are close to each other and were determined as 8,905±0,483. Whereas the PIC values ranged from between 0,507-0,794 at an average of 0,705. The He and Ho values used to calculate genetic diversity were determined on average as 0,734±0,023 and 0,509±0,038 respectively. According to the Fisher-Exact test result, the populations are not in HWE (P<0.001). Whereas the FIS values that show inter- population genetic diversity were determined as 0,353±0,092 (KRY), 0,328±0,095 (MRK) and 0,237±0,059 (AKK) respectively. In addition, Population Assignment and Principal Coordinate Analysis graphs were formed. When the pairwise FST and Nm, NeiuD,SHUA and G Statistics values used to calculate the genetic similarities and differences are examined, it is observed that the closest relations in terms of genetics is among AKK-KRY followed respectively by AKK-MRK and KRY-MRK.

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  AtıfScopus5·WoS5·OpenAlex4

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