Sürdürülebilir Kalkınma Amaçları

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Yazarlar

• Aliyazicioglu, Rezzan(1)
• Aliyazicioglu, Yuksel(1)
• Baltacı, Cemalettin(1)
• Demir, Selim(1)
• Misir, Sema(1)
• Ozkan, Mehtap Tugce(1)
• Turan, Ibrahim(1)
• Yildirmis, Sermet(1)
• Yüksel, Ferhat(1)
• İlyasoğlu, Huri(1)

• MakaleAçık Erişim2017Scopus SJRQ2/r/gumushane/15144
  Phenolic characterisation and antioxidant activity of Primula vulgaris and its antigenotoxic effect on fibroblast cells

  Ozkan, Mehtap Tugce; Aliyazicioglu, Rezzan; Demir, Selim; Misir, Sema; Turan, Ibrahim; Yildirmis, Sermet; Aliyazicioglu, Yuksel

  Background: Primula vulgaris has been used in traditional treatment, and its biological functions are attributed to its polyphenolics content. Objectives: The aim of this study was to determine the phenolics composition and the antioxidant activity of water extract of P. vulgaris (WEP) and to determine its probable preventive effects against H2O2-induced DNA damage in human fibroblast cells. Methods: The total polyphenolic content (TPC), ferric reducing antioxidant power (FRAP), and radical scavenging activity of WEP were determined using spectrophotometric methods. Phenolic compounds and antigenotoxic effects of WEP were evaluated using HPLC and comet assay, respectively. Results: The TPC and FRAP values of WEP were 15.023 ± 0.84 mg gallic acid and 82.63 ± 0.31 μM trolox per g sample, respectively. ρ-coumaric acid and rutin were detected as major phenolics. Moreover, WEP reducedH2O2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to the positive controls (only 20 μMH2O2 treatment). Conclusions: Primula vulgaris can be used in food, cosmetics, and drug industries because of its antioxidant and antigenotoxic activities.

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  AtıfScopus26·WoS30·OpenAlex25

  İncele
• MakaleYalnız künye2013Scopus SJRQ1/r/gumushane/15702
  Single-Laboratory Validation for the Determination of Aflatoxin B1, B2, G1, and G2 in Foods Based on Immunoaffinity Column and Liquid Chromatography with Postcolumn Derivatization and Fluorescence Detection

  Baltacı, Cemalettin; İlyasoğlu, Huri; Yüksel, Ferhat

  This study presents a method validation procedure for the determination of aflatoxin B1, B2, G1, and G2 in hazelnut, hazelnut paste, walnut, peanut, pistachio, corn, and wheat. The method consisting of clean-up with immunoaffinity column, high performance liquid chromatography with postcolumn derivatization and fluorescence detection was validated in accordance with Commission Regulation 2004/882/EC. The selectivity, linearity, decision limit, detection capability, detection and quantification limits, precision, recovery, ruggedness, and measurement uncertainty of the method were determined. The limit of detection and limit of quantification values (μg/kg) were: aflatoxin B1, 0. 02, 0. 07; aflatoxin B2, 0. 01, 0. 02; aflatoxin G1, 0. 02, 0. 07; and aflatoxin G2, 0. 01, 0. 03. The relative standard deviation values for the repeatability and within-laboratory reproducibility were below 4 and 5 %, respectively. The recovery values of the spiked samples ranged from 80 to 105 %. These results complied with minimum performance criteria established by regulation 2006/401/EC. Therefore, the procedure can be implemented for the routine analysis of aflatoxins in the studied matrices.

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  AtıfScopus12·WoS11·OpenAlex15

  İncele