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- MakaleAçık Erişim2025Scopus SJRQ1A strategy for the one-pot direct production of 5-hydroxymethylfurfural (HMF) from sucrose using organic weak acid in situ as a catalyst
Gül, Abdulkadir; Tanyıldızı, Muhammet Şaban
5-Hydroxymethylfurfural (HMF), which can be produced in high yields by acid-catalyzed dehydration of carbohydrates, is used as a feedstock in biofuel and biopolymer production. However, these acidic catalysts are usually added externally, which increases HMF production costs. Furthermore, other important parameters for high yields of HMF production are raw material selection, reaction medium, and separation-purification processes. The aim of this study is to produce HMF in one-pot without the addition of any exogenous catalyst by evaluating the fructose remaining in the production medium in which the glucose from sucrose hydrolysis is microbially fermented into gluconic acid (GA). HMF production from fructose in a biphasic system consisting of a fermentation liquid containing GA and fructose and 2-Methyltetrahydrofuran (2-MeTHF) was optimized using Central Composite Design (CCD). The variables examined included reaction time, reaction temperature, solvent type, salt type, and organic/aqueous phase ratio. In the study, by chelating GA and calcium ions, the low degree of ionization of GA was increased, and HMF was produced in high yields without the addition of a catalyst. Under optimum conditions, using 2-MeTHF/ fermentation liquid (6:1) and 7.56 wt% CaCl2, the yield of HMF produced in pressurized (hydrothermal) vessels at about 147 °C for 158 min reached 77.6 % of the theoretical yield. The amount of GA in the production medium was 92.64 % preserved from degradation. GA and HMF in different phases of the biphasic system were purified from the production medium in high yields. Furthermore, 2-MeTHF, which constitutes the organic phase of the biphasic system, was recovered at approximately 95 % by vacuum evaporation. As a result, HMF production from sucrose, which is a more economical and abundant raw material compared to glucose and fructose, was realized in one-pot without the addition of any catalyst.
AtıfScopus6WoS5OpenAlex7İncele - MakaleAçık Erişim2024Scopus SJRQ2Gluconic Acid Production by Using Recombinant Escherichia coli Waksman pqq+ Cells with a Novel Approach from Biomass Sources
Gül, Abdulkadir; Tanyıldızı, Muhammet Şaban
Gluconic acid (GA) is widely used in the pharmaceutical, food, detergent, textile, leather, and concrete industries. However, cost-effective and high-yield production of GA remains a challenge. Due to currently high raw material inputs of GA, various alternative carbohydrate sources are being investigated. Sucrose is one of the cost-effective biomass sources that can be used as feedstock. The most common industrial production of GA is based on wild-type bacteria and fungi, but there are many problems with this production. This study aimed to optimize the production of GA from glucose produced by hydrolysis of sucrose using recombinant E. coli Waksman (W) pqq+ strain. After sucrose was enzymatically hydrolyzed, significant medium components for GA production were determined as glucose, calcium carbonate (CaCO3), peptone, and ammonium phosphate ((NH4)3PO4) using Placket-Burman Design (PBD). Detailed optimization of the medium components that are significant in GA production was carried out using central composite design (CCD), and optimum values of the independent variables examined in maximum GA production (93.5 ± 2.95 g/L) were determined as glucose 95, CaCO3 25, peptone 2, and (NH4)3PO4 1.13 (g/L). Using results obtained in the Erlenmeyer experiments, GA production in the bioreactor was investigated by CCD. The maximum GA efficiency (3.20 ± 0.15 g/L. h) was obtained under conditions where the air supply rate was 10.82 L/min, stirring speed was 656.87 rpm, and CaCO3 concentration was 16.90 g/L. In conclusion, it has been shown that GA can be produced with a high yield with this novel approach using a recombinant strain for GA production from sucrose.
AtıfScopus5WoS5OpenAlex5İncele