Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Acet, Tuba(2)
- Özcan, Kadriye(2)
- Aslan, Rukiye(1)
- Harmankaya, Ahmet(1)
- Kapancık, Serkan(1)
- Kaya, Afşin A.(1)
- Kaya, Elif C¸elenk(1)
- Kocyigit, Umit M.(1)
- Kotiloğlu, Deniz(1)
- Lukšič, Miha(1)
- Medetalibeyoğlu, Hilal(1)
- Sefalı, Abdurrahman(1)
- Topal, Fevzi(1)
- Tüzün, Burak(1)
- Yüksek, Haydar(1)
- Yılmaz, Yonca(1)
- Öztürk Sarikaya, Sevim B.(1)
- Şentürk, Murat(1)
Yayınlar
- MakaleYalnız künye2026Novel 1,2,4-triazole-based n-mannich derivatives: Synthesis, spectroscopic characterization, cytotoxic, antimicrobial, and enzyme inhibitory activities, and in silico studies
Yılmaz, Yonca; Medetalibeyoğlu, Hilal; Kapancık, Serkan; Tüzün, Burak; Lukšič, Miha; Aslan, Rukiye; Topal, Fevzi; Kocyigit, Umit M.; Yüksek, Haydar; Harmankaya, Ahmet
A series of novel 1,2,4-triazole-based Mannich bases was designed, synthesized, and evaluated for biological and theoretical properties. Schiff base intermediates (C1–6) were prepared by condensing 4-benzoxy-3-methoxybenzaldehyde with various 3-substituted-4-amino-4,5-dihydro-1H-1,2,4-triazole-5-ones. Mannich reactions with formaldehyde and 2,6-dimethylmorpholine produced six target compounds (D1–6). Structures were confirmed using spectroscopic techniques. Following structural confirmation, the compounds showed significant cytotoxicity against HT-29 and MCF-7 cancer cell lines in MTT assays. Enzyme inhibition studies demonstrated activity against glucose-6-phosphate dehydrogenase (G6PDH) and lactate dehydrogenase (LDH), indicating potential relevance to cancer metabolism. Moderate acetylcholinesterase inhibition was observed, with IC50 values from 56.81 to 182.40 µM. Antimicrobial testing showed that D1, D3, D4, and D5 were active against selected microbial strains, while D2 and D6 were inactive. Density functional theory (DFT) calculations, molecular docking, ADME/T predictions, and molecular mechanics simulations were conducted to complement the experimental findings. These computational analyses indicated favorable binding interactions and pharmacological profiles.In summary, the newly synthesized triazole-based Mannich derivatives represent promising candidates for further pharmacological investigation.
- MakaleYalnız künye2021Biological activity of Psephellus appendicigerus as a natural agent
Acet, Tuba; Özcan, Kadriye; Sefalı, Abdurrahman
Psephellus appendicigerus, is a plant endemic to Turkey, belonging to the Asteraceae family, which is traditionally used in folk medicine in Turkey. The aim of this study is to reveal for the first time the biological activities of this plant and evaluate its potential use as a natural product. For this reason, different extracts (ethanol, methanol, ethyl acetate, and hexane) of the plant’s aerial parts were used in determining the antioxidant (2,20-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid and 1,1-diphenyl-2-picrylhydrazyl assays), antimicrobial (disc diffusion and microdilution methods), and enzyme inhibition activity (calorimetric methods) related to diabetes (α-amylase and α-glucosidase). Moreover, the phenolic constituents were analysed by high performance liquid chromatography. Methanol extract of the plant exhibited significant biological activity compared to other extracts. This extract had the highest amount of chlorogenic acid as phenolic compound. In addition, all extracts showed inhibition against at least one test microorganism. According to the results, P. appendicigerus has the potential to be used as a natural antioxidant, antimicrobial and enzyme inhibitor.
- MakaleYalnız künye2020Phytochemical profile and biological activity of a therapeutic orchid from Anatolia: Dactylorhiza romana subsp. georgica
Kotiloğlu, Deniz; Acet, Tuba; Özcan, Kadriye
In Anatolia, the tubers of Dactylorhiza romana subsp. georgica orchid are consumed as food and frequently used in folk medicine to alleviate the symptoms of some disorders. However, these properties have not been scientifically studied until the present day. In this research, we aimed to identify phenolic profile of the orchid tubers and determine their several features and functions. Among these, there are the antioxidant and antimicrobial effects and the inhibitory effect against diabetes related enzymes (α-amylase and α-glucosidase). In this sense, total phenolic content (Folin–Cicalteu assay), total antioxidant capacity (Phosphomolybdate method), antioxidant (ABTS and DPPH assays) and enzyme inhibition effects of tuber extracts obtained with different solvents (chloroform, ethyl acetate, methanol, ethanol, or hexane) were determined by using spectrophotometric methods, and the phenolic profile was also analysed by HPLC. In addition, antimicrobial activity of the extracts against 3 g (+) and 3 g (−) bacteria and 2 forms of yeasts was determined by disc diffusion and micro dilution methods. Based on our findings, the total phenolic content and antioxidant properties of ethyl acetate extract were considerably higher than the others. The most abundant among the screened phenolic standards was found to be benzoic acid. The highest α-amylase and α-glucosidase enzyme inhibition was measured in methanol and chloroform extracts, respectively. The highest antibacterial (B. cereus) and anticandidal (C. tropicalis and C. albicans) activities were detected in the chloroform and ethyl acetate extracts, respectively. In short, it was clearly seen that the D. romana subsp. georgica tuber had a great potential for being used in food and drug industries as a source of bioactive compounds.
- MakaleAçık Erişim2015Synthesis and determination of some biological activities of novel 2,4-dinitrophenyl derivatives
Öztürk Sarikaya, Sevim B.; Kaya, Afşin A.; Kaya, Elif C¸elenk; Şentürk, Murat
The antioxidant and radical scavenging activities of the synthesized compounds 3, 5, and 6 were determined by various in vitro assays such as 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid radical (ABTS.+) scavenging, ferric ion (Fe3+) reducing power and ferrous ion (Fe2+) chelating activities. Moreover, these activities were compared to those of standard antioxidants such as butylated hydroxyanisole, butylated hydroxytoluene, and trolox. The results showed that the new compounds (3, 5, and 6) had potential antioxidant activity. Besides, inhibition of the two human cytosolic carbonic anhydrase (hCA, EC 4.2.1.1) isozymes I and II with some nitrobenzene compounds was investigated. Compounds 1-6 showed Ki values in the range of 4.88-193.4 μM and 5.295-54.75 μM for hCA I and hCA II, respectively.