Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Akay, Seref(2)
- Guo, Zheng(2)
- Kong, Fanbin(2)
- Ozturk, Samet(2)
- Singh, Rakesh K.(2)
- Zhang, Jin(2)
- Arserim-Uçar, Dılhun Keriman(1)
- Ayna, Adnan(1)
- Heils, Rene(1)
- Ilyasoglu, Huri(1)
- Mesci Dindar, Esra(1)
- Nadzieja, Marcin(1)
- Smirnova, Irina(1)
- Trieu, Hoc Khiem(1)
- Yesil-Celiktas, Ozlem(1)
- Özdilek, Hasret(1)
- İlyasoğlu, Huri(1)
Yayınlar
- MakaleYayıncıda açık erişim2026Scopus SJRQ1Chitosan-Based Colloidal Delivery Systems as Natural Carriers for Propolis: Physicochemical Characterization and Application in Walnut Sausages
Mesci Dindar, Esra; Arserim-Uçar, Dılhun Keriman; Ayna, Adnan
In this study, a chitosan-based colloidal delivery systems (CDs) was developed as a natural delivery system for phenolic compounds from propolis extracts obtained via accelerated solvent extraction (ASE) and evaluated for improving the functional properties of walnut sausages. ASE propolis extract was incorporated into a chitosan (C)-based colloidal delivery system formulated with varying concentrations of diacetyl tartaric acid ester of mono- and diglycerides (DATEM) and lecithin (L). The formulation containing 2.5% chitosan, 12% DATEM and propolis, with a particle size of 265.9 nm and a ζ-potential of +40.8 mV, was selected for further characterization and food applications. The phenolic encapsulation of CDs depended on the structural characteristics of individual phenolic compounds, with chrysin (70.51%), vanillin (65.12%), and benzoic acid (51.26%) exhibiting the highest encapsulation; CD-treated walnut sausages decreased the lipid oxidation and improved the storage stability of the walnut sausages during storage. These findings indicate that the chitosan-based colloidal delivery system is a promising carrier for improving the encapsulation of propolis phenolics in walnut sausages.
- MakaleAçık Erişim2024Scopus SJRQ2Design and fabrication of low-cost microsystems for production of chitosan particles; [Kitosan parçacıklarının üretimi için düşük maliyetli mikrosistemlerin tasarımı ve fabrikasyonu]
Akay, Seref; Özdilek, Hasret
Recent research has focused on the methods for administration of medications in a controlled manner while minimizing side effects. Cross-linked hydrogels have wide application area as nanocarriers. There are many approaches for developing suitable methods to produce ideal particles. Advances in microtechnology have been considered as a solution to produce controlled drug delivery systems, however, it is limited due to the necessity for specialized laboratories. In this study, a simple method to produce antibiotic-loaded chitosan particles is proposed. Production was carried out with two different designs, a syringe-based flow focusing system (outer diameter of 1 mm, inner diameter of 120 µm) and microchannel with 250 µm width and 100 µm height fabricated with the computer numerical control (CNC). Sodium Triphosphate (TPP) was used as cross-linking agent for the production of particles and the effects of the concentration and flow rates on the particle formation was investigated. It has been observed that particles can be produced in a syringe-based system, but the resulting particles are not homogeneous. In the studies carried out with CNC microchannel, it was determined by SEM analysis that the obtained particles ranged in size from 39 to 771 nm, and it was seen that the CNC microchannel was more effective at low and equal flow rates (25 µl/min). It has been found that increasing the flow rate and concentration of chitosan increases the particle size. It was determined that Ampicillin loaded particles synthesized with the CNC microchannel exhibited high inhibition against Gram positive and Gram negative bacteria. These results show that the proposed approaches for the design of microsystems can be used in the formation of drug carriers without the need for specialized laboratories and expertise.
AtıfScopus1
İncele - MakaleAçık Erişim2021Scopus SJRQ1Effect of cellulose nanofiber-based coating with chitosan and trans-cinnamaldehyde on the microbiological safety and quality of cantaloupe rind and fresh-cut pulp. Part 2: Quality attributes
Ozturk, Samet; Zhang, Jin; Singh, Rakesh K.; Kong, Fanbin
The quality characteristics of fruits and vegetables can be protected with coatings. The presented study aimed to evaluate the effect of cellulose nanofiber (CNF) based coatings with chitosan (CHI) and trans-cinnamaldehyde (TC) on the quality attributes, including weight loss, total soluble solid (TSS) content, firmness, and color of whole and fresh-cut cantaloupe during storage at 22 °C and 4 °C, respectively. Results showed that CNF-based coating was effective in protecting whole cantaloupes from firmness reduction and color change in redness/greenness (a*) and yellowness/blueness (b*) during room-temperature storage for up to 11 days. Coating containing only CNF was not able to prevent the quality changes of fresh-cut cantaloupe during the cold storage, but CNF + CHI/CNF + CHI + TC significantly decreased the weight loss, decelerated the firmness increase and maintained the lightness (L*) of fresh-cut cantaloupe for up to 7 days. The incorporation of chitosan (1%, w/w) improved the protection effect of CNF-based coatings against quality changes during storage for both whole and fresh-cut cantaloupes, but TC (1%, w/w) did not show significant enhancement on the protection effects of coatings for most quality attributes. This study indicates that the CNF-based coatings have the potential to maintain quality attributes of whole and fresh-cut fruits during storage and distribution.
AtıfScopus12WoS8OpenAlex9İncele - MakaleAçık Erişim2020Scopus SJRQ1Effect of cellulose nanofiber-based coating with chitosan and trans-cinnamaldehyde on the microbiological safety and quality of cantaloupe rind and fresh-cut pulp. Part 1: Microbial safety
Zhang, Jin; Ozturk, Samet; Singh, Rakesh K.; Kong, Fanbin
This study aimed to evaluate the effect of cellulose nanofiber (CNF)-based coating containing chitosan (CHI) and trans-cinnamaldehyde (TC) on microbial growth of foodborne pathogens and spoilage microorganisms on cantaloupe rind and fresh-cut pulp. Coated/uncoated cantaloupe rind and pulp samples were inoculated with Salmonella enterica, Escherichia coli O157:H7 and Listeria monocytogenes and subsequently subjected to storage for 20 d at 4 °C or 12 d at 22 °C. Results showed that coating with only CNF (0.5%, dry-base) was effective in the elimination of S. enterica and E. coli O157:H7. Adding CHI (1%, w/w) and TC (1%, w/w) into CNF-based coating enhanced film formation on the surface and increased the antimicrobial activity on rind sample, but the reduction effect was less significant on pulp samples. The CNF (0.5%, dry-base) +CHI (1%, w/w) +TC (1%, w/w) coating resulted in 2.23, 2.43 and 3.22 log10 CFU/cm3 reduction of S. enterica, E. coli O157:H7 and L. monocytogenes, respectively, on pulp samples after 20 d at 4 °C. The formulation also significantly inhibited the growth of natural spoilage microorganisms, and no growth was observed up to 18 d at 4 °C. Results indicated that CNF-based coating significantly improved microbial safety of cantaloupe rind and fresh-cut pulp during storage by forming a physical barrier reducing activities of oxygen-dependent microorganisms.
AtıfScopus13WoS9OpenAlex10İncele - MakaleYalnız künye2019Scopus SJRQ1Caffeic acid grafted chitosan as a novel dual-functional stabilizer for food-grade emulsions and additive antioxidant property
Ilyasoglu, Huri; Nadzieja, Marcin; Guo, Zheng
The aim of this study was to develop a novel dual-functional stabilizer for food-grade Pickering emulsions by grafting caffeic acid onto chitosan via a free radical mediated method. The grafting of caffeic acid onto chitosan was confirmed by UV-vis absorption spectra, FTIR spectra and H-1 NMR spectra and DSC thermogram. The antioxidant properties of the caffeic acid grafted chitosan (CA-g-Ch) were measured by three in vitro assays. Caffeic acid-grafted chitosan displayed much better Fe2+ chelating property than both chitosan and caffeic acid, and retained comparable DPPH radical scavenging activity as caffeic acid. Moreover, the formation of the CA-g-Ch based emulsion was monitored by the turbidity, zeta potential, and particle size measurements. The CA-g-Ch polymers were found to be pH-responsive. The oil (linoleic acid) in water emulsions with the CA-g-Ch (as emulsifier/Pickering stabilizer) was prepared to examine its potential to stabilize Pickering emulsions, as well as its additive antioxidant properties. The results (zeta potential, mean droplet size, creaming index and TBARS values etc) demonstrated that the CA-g-Ch could act as a dual-functional stabilizer for Pickering emulsions. The emulsions made with the CA-g-Ch were found to be stable at a broad pH range of 3-6. Our findings proved that CA-g-Ch as novel dual-functional polymeric surfactant, may have great potential to find applications in food and pharmaceutical industries.
AtıfWoS112OpenAlex120İncele - MakaleYalnız künye2019Scopus SJRQ1Water soluble chitosan-caffeic acid conjugates as a dual functional polymeric surfactant
İlyasoğlu, Huri; Guo, Zheng
A water soluble chitosan-caffeic acid conjugate (C-CAC) was prepared by chemical modification of chitosan using activated caffeoyl with dimethyl aminopyridine as a catalyst. The C-CAC was characterized using UV–visible spectrometry, Fourier transform infrared, nuclear magnetic resonance spectroscopies and differential scanning calorimetry. The caffeoyl content in the C-CAC was 65 mg/g. To study the introduction of a new function into chitosan, the antioxidant activities of the synthesized C-CAC was determined using three in vitro assays: DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and ferric reducing antioxidant power. The half inhibition concentration values for DPPH and ABTS radicals were 370 and 172 μg/ml, respectively; showing its good radical scavenging activity. The emulsifying properties of the C-CAC were investigated by preparing 10% fish oil-in-water emulsions. C-CAC not only showed a good ability to stabilize oil-in-water emulsions, but also was more effective in retarding lipid oxidation compared to chitosan; implying that the synthesized C-CAC was functioning in an oil-in-water emulsion system as both emulsifier and antioxidant. Chitosan-phenolic conjugates, as a biopolymer-based surfactant, may represent a promising ingredient for enabling stable oxidation-sensitive oil-in-water emulsions.
AtıfScopus45WoS43OpenAlex44İncele - MakaleYalnız künye2017Scopus SJRQ1An injectable alginate-based hydrogel for microfluidic applications
Akay, Seref; Heils, Rene; Trieu, Hoc Khiem; Smirnova, Irina; Yesil-Celiktas, Ozlem
The objective of this study was to develop an injectable alginate based formulation for immobilizing enzymes into microfluidic systems. The gelation was induced upon lowering the pH by addition of D-glucono-δ-lactone (GDL) and release of Ca+ ions from solid CaCO3. The effects of GDL concentration on enzymatic activity and gelation time were investigated. The results indicated that increasing the GDL concentration increased both surface area and enzymatic activity. Also, chitosan was added to the formulation at different ratios to enhance the stability of enzyme during immobilization. For microfluidic application, 100 μl spiral coil single channel microchip was fabricated and alginate GDL mixture containing β-glucosidase was injected to the microchannel prior to gelation. Enzymatic conversion was performed by pumping substrate (pNPG) through the microchannel. The results indicated that the entire substrate was converted continuously during 24 h without any leakage or deactivation of immobilized enzyme.
AtıfScopus42WoS34OpenAlex38
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