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- MakaleYalnız künye2026First molecular data based on the mitochondrial 16S rRNA gene for the endemic harvestman Mediostoma izmiricum Snegovaya, Kurt and Yağmur (Opiliones: Nemastomatidae) from Türkiye, with insights into the phylogenetic relationships of the genus
Kurt, Pınar
Mediostoma izmiricum Snegovaya, Kurt and Yağmur, is a species of the family Nemastomatidae Simon, and is endemic to Türkiye. To date, there is no molecular data available for the 16S rRNA gene region for this species or its genus, which limits our knowledge of its phylogenetic position. The 16S rRNA gene region of this species was analysed for the first time, yielding a sequence length of 465 bp. Bayesian inference (BI) and maximum likelihood (ML) phylogenetic trees were subsequently constructed based on these data, revealing the phylogenetic position of both the species and its genus.
- MakaleAçık Erişim2025First Molecular Data for the Genus Kovalius (Opiliones: Sclerosomatidae: Leiobuninae) and their Phylogenetic Relationships; [Kovalius (Opiliones: Sclerosomatidae: Leiobuninae) Cinsinin İlk Moleküler Verileri ve Filogenetik İlişkileri]
Kurt, Pınar; Yildirim Doğan, Nalan
Kovalius (Opiliones: Sclerosomatidae: Leiobuninae) is a small genus of harvestmen described by Tchemeris, 2023. The first description of Kovalius logunovi was made from Russia and it was subsequently recorded from Türkiye on the basis of the morphological data. The use of morphological data alone for the identification of taxa and determination of their relationships may lead to limitations and difficulties in some cases. As a result, the use of molecular data in addition to the morphological data in the identification of new taxa and determination of phylogenetic relationships increases the reliability of studies. In this study, the mitochondrial 16S rRNA gene region of the Kovalius logunovi species was examined for the first time and a sequence of approximately 408 bp was obtained. Based on these data, the phylogenetic relationships of the species with similar species were revealed.
- MakaleAçık Erişim2019Presence of different bacterial species in thermal sources and novelty in their industrial enzyme productions
Albayrak, Seyda; Genc, Berna; Ozkan, Hakan; Taskin, Mesut; Adiguzel, Ahmet
In this study, one hundred and thirty isolates were isolated from water and sludge samples taken from hot springs located in different regions of Turkey. Among them, eleven isolates were chosen according to conventional (morphological, physiological and biochemical tests) and molecular methods (rep-PCR and 16S rRNA sequencing). These bacteria were then tested for their capability to produce valuable enzymes. As a result; species belonging to Bacillus, Anoxybacillus, Aeribacillus, Enterococcus, Exiguobacterium and Paenibacillus were identified. Test strains were found to have optimum reproductive potential at pH 5.0-9.0 and 15-65°C, usually at a concentration of 1.0-10.0% (w/v) NaCl. In addition, all thermotolerant bacteria were Gram, endospore (except E. profundum), catalase and oxidase (except E. faecium and E. profundum) positive, and rod-shaped (except E. faecium). It was observed that all isolates had a 99% similarity percentage as a result of 16S rRNA sequence analysis. All of the isolates were capable of producing industrially important enzymes moreover, eight of them could produce at least two of these enzymes. Test strains had high potential of industrial enzyme production, and the enzymes from these thermo-tolerant isolates will be widely used in biotechnological processes.