Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2019Centaurea hypoleuca DC: Phenolic content, antimicrobial, antioxidant and enzyme inhibitory activities
Özcan, Kadriye; Acet, Tuba; Çorbacı, Cengiz
Centaurea hypoleuca is one of the plants have been traditionally used in treatments of many diseases in folk medicine in Turkey. In the present study, ethanol, methanol, and ethyl acetate extracts of the plant's aerial parts were examined and their antioxidant, antimicrobial, and enzyme inhibition activities were determined for the first time. The antioxidant assays were performed in vitro using ABTS and DPPH methods, whereas the antimicrobial efficiency was determined using disc diffusion and microdilution methods. Moreover, the enzyme inhibition activity was analyzed using calorimetric methods targeting the key enzymes used in the treatment of Alzheimer's disease (cholinesterase) and diabetes (glucosidase and amylase). Also, the phenolic contents were analyzed using HPLC. The biological activity of flower ethyl acetate extract was found to be significantly higher than those of other extracts. This extract was more prominent because of its strong antimicrobial activity with the MIC value of 8 µg mL−1 against MRSA. Moreover, the main components of the extract were determined to be catechin and chlorogenic acid. In conclusion, these results indicate that C. hypoleuca can be pharmaceutically used as herbal remedies for its antioxidant, antimicrobial, and enzyme inhibitor effects.
- MakaleYalnız künye2018Determination of Antioxidant and Antimicrobial Properties of Lady's Mantle (Alchemilla ellenbergiana) Extracts
Ozcan, Kadriye; Acet, Tuba
In this study, different polarity solvent (hexane, ethyl acetate, ethanol and methanol) extracts of Alchemilla ellenbergiana (Lady's Mantle), which used in folk medicine plant in Gümüşhane region, were investigated in terms of total phenolic contents, antioxidant and antimicrobial activity. Total phenolic contents were measured by spectrophotometric metod as gallic acid equivalent and detect in the highest in ethanol and methanol extracts. Antioxidant activity of extracts were determined by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods by spectrophotometric metod and calculated as trolox equivalent. Antimicrobial activity was tested against to pathogen microorganisms by disc diffusion method. As a result, it has been found that ethanol and methanol extracts of plant have high antioxidant and antimicrobial activity (p<0.05). Similarly, it has been found that antimicrobial activity of ethanol and methanol extracts higher than others
- MakaleYalnız künye2018ANTIMICROBIAL AND ANTIOXIDANT SCREENING, SYNERGY STUDIES OF HELICHRYSUM CHIONOPHILUM EXTRACTS AGAINST TO RESISTANT MICROBIAL STRAINS
Ozcan, Kadriye; Acet, Tuba
In this study, the antimicrobial activities and antioxidant properties of five different extracts of Helichrysum chionophilium plants from Gumushane/Turkey were investigated. To determine the antimicrobial activity disc diffusion and microdilution methods were used against to fifteen microorganisms. The effect of plant extracts and antibiotic combinations against to vancomycin-resistant Enterococcus faecium and methicillin-resistant Staphylococcus aureus were investigated as well. Total phenolic and flavonoid contents were determined by spectrophotometric techniques. Antioxidant properties of plant extracts evaluated by free radical scavenging assays which ABTS and DPPH. The plant ethyl acetate extract showed the highest level of total phenolic (423.01 +/- 0.8 mg GAE/g extract) and flavonoid contents (100.18 +/- 1.4 mg QE/g extract). Similarly, ethyl acetate extract exhibited the highest level of DDPH (IC50: 67.44 +/- 0.9 mu g/ml). However, hexane extract exhibited the highest level activity of ABTS (IC50: 55.20 +/- 1.3 mu g/ml). The highest MIC value was determined as 8 mu g/ml against to Pseudomonas aeruginosa on ethyl acetate extract. Besides, all of extracts exhibited antimicrobial activity on at least one test organism. Plant extracts with some antibiotic combinations generated synergies or partial synergic activity against to VREF and MRSA. These findings revealed that the plant has strong antimicrobial and antioxidant properties. Finally, using the plant extracts and antibiotic combinations might be a good tool in fighting with antibiotic resistance strains.
- MakaleYalnız künye2018Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis
Ali, Muhammad Amjad; Azeem, Farrukh; Nawaz, Muhammad Amjad; Acet, Tuba; Abbas, Amjad; Imran, Qari Muhammad; Shah, Kausar Hussain; Rehman, Hafiz Mamoon; Chung, Gyuhwa; Yang, Seung Hwan
Plant WRKY transcription factors play a vital role in abiotic stress tolerance and regulation of plant defense responses. This study examined AtWRKY11 and AtWRKY17 expression under ABA, salt, and osmotic stress at different developmental stages in Arabidopsis. We used reverse transcriptase PCR, quantitative real-time PCR, and promoter:GUS lines to analyze expression. Both genes were upregulated in response to abiotic stress. Next, we applied the same stressors to seedlings of T-DNA insertion wrky11 and 17 knock-out mutants (single and double). Under stress, the mutants exhibited slower germination and compromised root growth compared with the wild type. In most cases, double-mutant seedlings were more affected than single mutants. These results suggest that wrky11 and wrky17 are not strictly limited to plant defense responses but are also involved in conferring stress tolerance.
- MakaleYalnız künye2018In vitro antioxidant and antimicrobial activities of the five different solvent extracts of Centaurea pulcherrima var. Freynii from Turkey
Ozcan, Kadriye; Acet, Tuba
Various extracts of Centaurea pulcherrima var. freynii were investigated for total phenolic, flavonoid, antioxidant and antimicrobial activities. The antioxidant properties of plant extracts were evaluated by free radical scavenging assays (DPPH and ABTS). Antimicrobial activities were examined using disc diffusion methods. According to the results of antioxidant capacity assays, the plant showed moderately antioxidant activity in test methods. It was found that ethyl acetate extract showed the most effective activity including total phenolic, flavonoid, antioxidant and antimicrobial activities.
- MakaleYalnız künye2017Gümüşhane İlinde Tıbbi la Kullanılan Atkuyruğu (Equisetum arvense) Bitkisinin Bazı Biyolojik Aktivitelerinin İncelenmesi#
Ozcan, Kadriye; Acet, Tuba
Bu çalışmada, Gümüşhane ilinde halk tarafından farklı tıbbi amaçlarla kullanılmakta olan atkuyruğu (Equisetum arvense) bitkisinin farklı polariteye sahip çözücülerle elde edilmiş ekstrelerinin toplam fenolik miktarı, antimikrobiyal ve antioksidan aktiviteleri incelenmiştir. Toplam fenolik miktarı gallik asit eşdeğeri olarak spektrofotometrik yöntemle ölçülmüştür. Antimikrobiyal aktivite, disk difüzyon ve mikrodilüsyon (MIC değeri) yöntemleriyle belirlenmiş; antioksidan aktivite ise ABTS [(2,2'-azino-bis (3- etilbenzotiazolin-6-sülfonik asit)] ve DPPH (2,2-difenil-1-pikrilhidrazil) yöntemleri kullanılarak tespit edilmiş ve troloks eşdeğeri olarak hesaplanmıştır. Toplam fenolik miktarı, en yüksek etil asetat ekstresinde (108,9 mgGAE/g ekstre) tespit edilirken, en yüksek antioksidan kapasite etanol ekstresinde 15,76 µg/ml troloks eşdeğeri olarak bulunmuştur. Ayrıca, en yüksek antimikrobiyal aktivite etanol ekstresinde MRSA'ya karşı 4 µg/ml MIC değeri olarak tespit edilmiştir.
- MakaleAçık Erişim2014The Arabidopsis thaliana FASCICLIN LIKE ARABINOGALACTAN PROTEIN 4 gene acts synergistically with abscisic acid signalling to control root growth
Seifert, Georg J.; Acet, Tuba; Xue, Hui
Background and Aims The putative FASCICLIN-LIKE ARABINOGALACTAN PROTEIN 4 (At-FLA4) locus of Arabidopsis thaliana has previously been shown to be required for the normal growth of wild-type roots in response to moderately elevated salinity. However, the genetic and physiological pathway that connects At-FLA4 and normal root growth remains to be elucidated. Methods The radial swelling phenotype of At-fla4 was modulated with growth regulators and their inhibitors. The relationship of At-FLA4 to abscisic acid (ABA) signalling was analysed by probing marker gene expression and the observation of the At-fla4 phenotype in combination with ABA signalling mutants. Key Results Application of ABA suppresses the non-redundant role of At-FLA4 in the salt response. At-FLA4 positively regulates the response to low ABA concentration in roots and is required for the normal expression of ABA-and abiotic stress-induced genes. The At-fla4 phenotype is enhanced in the At-abi4 background, while two genetic suppressors of ABA-induced gene expression are required for salt oversensitivity of At-fla4. Salt oversensitivity in At-fla4 is suppressed by the CYP707A inhibitor abscinazole E2B, and salt oversensitivity in At-fla4 roots is phenocopied by chemical inhibition of ABA biosynthesis. Conclusions The predicted lipid-anchored glycoprotein At-FLA4 positively regulates cell wall biosynthesis and root growth by modulating ABA signalling.