Effects of Varying Ratios of Hawthorn Fruit Extract on Nesfatin-1, GLP-1 and cTnI Response in Streptozotocin-induced Diabetic Rats

Background: The aim of this study was to investigate the ef fects of Hawthorn (Crataegus oxyacantha) Fruit Extract on the response of nesfatin-1, Glucagon-like peptide 1 (GLP-1) and cardiac troponin I (cTnI) in STZ-induced diabetic rats. Methods: This study utilized 64 adult male Wistar albino rats, aged 8-10 weeks and weighing an average of 195-215 g. Eight groups were randomly selected, each comprising eight rats designated as Control (C), H100, H200, H300; Diabetes Control (DC), DH100, DH200, DH300. Blood samples were collected from the tail vein (vena caudalis) of all individuals on days 0 and 21 of the research. The serum levels of Nesfatin-1, GLP-1 and cTnI were quantified and assessed using the ELISA technique. Result: In diabetic groups, the increase in serum mean nesfatin-1 and cTnI levels was most pronounced in DC groups, while the most significant decrease due to increased HFE supplementation was observed in DR200 groups (p<0.01); whereas in diabetic groups, the decrease in serum mean GLP-1 levels was observed in DC group, while the most significant increase due to increased HFE supplementation was detected in DG200 group. As a result, it was concluded that 200 mg/kg RPE administration to healthy and di abetic rats di d not cause any side eff ects. It was concluded that HFE, which has an anti-diabetic eff ect by regulating the inflammatory response that plays a role in the development of diabetes, decreasing nesfatin-1 levels and increasing GLP-1 levels, may be useful in supporting the impaired pancreatic beta cell function that occurs in diabetes.