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• MakaleAçık Erişim2025/r/gumushane/12401
  Protective effect of tarantula cubensis extract on ischemia-reperfusion injury in rat ovaries: Immunohistochemical, biochemical and histopathological evaluations

  Paksoy, Zahid; Ozturk Sarikaya, Sevim Beyza; Yumusak, Nihat

  Ischemia-reperfusion (IR) injury is a common pathology affecting the ovary. This study aimed to investigate the effect of Tarantula cubensis extract (TCE) on ovarian IR injury. Forty-two female rats were used and randomly divided into 7 groups: Control, Sham, IR, Ischemia+TCE+Reperfusion, TCE+IR, Ischemia+Ethanol+Reperfusion and Ethanol+IR. The control and sham groups did not receive any treatment. All other groups were subjected to 3 h of ischemia + 3 h of reperfusion. A single dose of TCE (0.2 ml Theranekron) was administered subcutaneously either before or after ischemia in the treatment groups. In the last 2 groups, ethanol was applied before or after ischemia. After reperfusion, blood samples were collected and analyzed for biochemical parameters. Additionally, the ovarian tissue was evaluated histopathologically and immunohistochemically. The IR group exhibited more marked hyperemia, hemorrhage, and necrosis. Both treatment groups showed histopathological improvement. Moreover, the recovery rate was greater in the animals given theranekron before ischemia. IR injury caused oxidative stress in the rats. However, Theranekron did not significantly reduce the MDA levels (p > 0.05). On the other hand, SOD levels were significantly increased in the treatment groups (p < 0.01). Compared to the control and sham groups, the IL-1β, IL-6 and TNF-α levels were very high in the IR group. Conversely, the immunoreactivity of these cytokines was further reduced in the animals given theranekron before ischemia. Our findings suggest that TCE may be beneficial in the treatment of ovarian IR damage, especially when Theranekron is given before ischemia.

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• MakaleYalnız künye2025/r/gumushane/12292
  Chemical Composition and Bioactivities of Sideritis libanotica Labill subsp. Libanotica: LC–HRMS Phytochemical Profiling, Mineral Content, Antioxidant, Antibacterial, and Neuroprotective–Antidiabetic Enzyme Inhibitory Potentials

  Öztürk Sarıkaya, Sevim Beyza; Zehiroğlu, Cuma

  Sideritis libanotica Labill. subsp. libanotica (SLL) is an herbal tea used for colds, coughs, and digestive system disorders. SLL has higher antioxidant activity in methods (µg/mL) like 1,1-diphenyl-2-picrylhydrazyl (DPPH•) (40.912 ± 0.170), ABTS•+ (14.61 ± 0.331) scavenging activity, and Fe3+ (0.872 ± 0.052) and Cu2+ (0.840 ± 0.013) reduction capacity compared to other Sideritis species. It is rich in essential minerals, such as Ca, Mg, and P, with the highest content of mineral K (14 203.97 mg/kg). Zone values in the range of 9–11 mm were obtained from Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Pseudomonas putida, and Klebsiella pneumoniae strains, which is an indicator that the plant has antibacterial activity. Among 33 secondary metabolites, sinapinic acid, rutin, and hispidulin have high values, whereas the one with the highest value is verbascoside (274 483 mg/kg), a pharmacologically active compound. It showed an inhibitory effect (mg/mL) on some enzymes targeting neurodegenerative and metabolic disorders. (IC50; acetylcholinesterase [AChE]: 0.065, butyrylcholinesterase [BChE]: 0.086, α-glucosidase: 0.014).

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• MakaleYalnız künye2023/r/gumushane/11786
  LC-HRMS Profiling and Phenolic Content, Cholinesterase, and Antioxidant Activities of Terminalia citrina

  Özler, Ebubekir; Topal, Fevzi; Topal, Meryem; Öztürk Sarıkaya, Sevim Beyza

  Terminalia citrina (T. citrina) belongs to the Combretaceae family and is included in the class of medicinal plants in tropical countries such as Bangladesh, Myanmar, and India. The antioxidant activities of lyophilized water (WTE) and alcohol extracts (ETE) of T. citrina fruits, their phenolic content by LC-HRMS, and their effects on cholinesterases (ChEs; AChE, acetylcholinesterase, and BChE, butyrylcholinesterase) were investigated. Especially ten different analytical methods were applied to determine the antioxidant capacity. Compared with similar studies for natural products in the literature, it was determined that both WTE and ETE exhibited strong antioxidant capacity. Syringe and ellagic acids were higher than other acids in ETE and WTE. IC50 values for ETE and WTE in DPPH radical and ABTS⋅+ scavenging activities were calculated as 1.69–1.68 μg mL−1 and 6.79–5.78 μg mL−1, respectively. The results of the biological investigations showed that ETE and WTE had an inhibition effect against ChEs, with IC50 values of 94.87 and 130.90 mg mL−1 for AChE and 262.55 and 279.70 mg mL−1 for BChE, respectively. These findings indicate that with the prominence of herbal treatments, T. citrina plant may guide the literature in treating Alzheimer's Disease, preventing oxidative damage, and mitochondrial dysfunction.

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• MakaleYalnız künye2023/r/gumushane/11556
  Endemic Mountain Tea (Sideritis dichotoma Huter): Antioxidant and Antibacterial Activities, Mineral Content by ICP-MS and Phytochemical Content by LCHR-MS

  Öztürk Sarıkaya, Sevim Beyza; Zehiroğlu, Cuma

  Endemic Sideritis dichotoma Huter, herbal tea, is used for colds, coughs and digestive ailments. Since at least 25 % of the active ingredients of pharmacological drugs are obtained from plants, it is important to investigate many plants, both traditionally used and whose potential hasnt yet been discovered, before they disappear. When the results compared to other Sideritis species, it has better antioxidant activity in DPPH⋅ scavenging activity, Fe3+ and Cu2+ reducing capacity methods and is also rich in minerals, necessary for human body, K, Mg, Ca, P, S. Zone values (10–19,5 mm) obtained on S. aureus, P. aeruginosa, E. coli, S.enteritidis, E. faecalis, P. putida and K. pnemoniae strains are an indication that the plant has antibacterial activity. 27 secondary metabolites were identified by LC-HRMS. Verbascoside, pharmacologically active compound known to have antitumor effect, cytotoxic selectivity and anticancer activity, is the most abundant phenolic in ethanol extract (49297.13 mg/kg).

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• Derleme MakaleAçık Erişim2022/r/gumushane/17116
  Spirulina: Özellikleri, Faydaları ve Sağlık-Besin İlişkisi

  Sarikaya, Sevim Beyza Ozturk; Budak, Burhan

  Spirulina, antioksidanlar, esansiyel amino asitler, yüksek kaliteli proteinler ve esansiyel doymamış yağlar gibi birçok faydalı içeriği nedeniyle bilimsel olarak birçok alanda kullanılan doğal bir üründür. Spirulina, A, C, E ve B vitaminleri ve kalsiyum, çinko, magnezyum ve selenyum gibi birçok mineral içeriğine sahip olduğu için besleyiciliği yoğun bir gıda olarak bilinir. Özellikle selenyum ve C vitamini antioksidan özelliklere sahip vitaminlerdir ve hücrelerimizi ve dokularımızı olası hasarlara karşı korurlar. Bu özelliklerinden dolayı Spirulina gıda, kozmetik ve tıp alanlarında kullanılmaktadır. Antioksidan özellikler içerdiğinden artrit, diyabet, anemi, kanser ve kardiyovasküler hastalıklar gibi çeşitli hastalıklarda kullanılır. Birçok çalışmada Spirulina'nın özellikleri araştırılmış ve olumlu sonuçlar elde edilmiştir. Ayrıca, Spirulina'nın farmakoloji, kozmetik, tıp ve gıda gibi alanlarda kullanımıyla da önemi artmıştır. Bu çalışmada, sayısız faydası bulunan bu mikroalglerin önemi günümüz yaklaşımlarıyla ve biyokimya, biyoteknoloji ve tıp alanlarında yapılan çalışmalarla vurgulanmaya çalışılmıştır.

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• MakaleYalnız künye2021/r/gumushane/14966
  In vitro Antioxidant and Cytotoxic Activities of Extracts of Endemic Tanacetum erzincanense Together with Phenolic Content by LC-ESI-QTOF-MS

  Yapıcı, İsmail; Altay, Ahmet; Öztürk Sarıkaya, Beyza; Korkmaz, Mustafa; Atila, Alptuğ; Gülçin, İlhami; Köksal, Ekrem

  In this study, phenolic composition, and in vitro biological activities of ethyl acetate (EAE) and methanol (ME) extracts obtained from the aerial parts of endemic Tanacetum erzincanense were investigated. Total phenolic and flavonoid content of the extracts were determined by Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. Antioxidant capacity of the extracts was evaluated over radical scavenging (DPPH and ABTS) and metal ion reducing power (FRAP and CUPRAC) tests. Individual phenolic compounds in ME was analyzed by high-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS). Cell inhibitory potential of the extracts was tested against colorectal adenocarcinoma (HT-29), breast adenocarcinoma (MCF-7), and hepatocarcinoma (HepG2) cells by 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. The results showed that ME contains higher TPC (64.4 mg GAE/g) and TFC (62.2 mg QE/g) than those of EAE (41.5 mg GAE/g and 40.0 mg QE/g). LC-ESI-QTOF/MS analysis revealed that ME is rich in phenolic compounds, namely, chlorogenic acid, apigenin, quercetin, luteolin, and diosmetin. Antioxidant assay results indicated that ME possess stronger activity than EAE and a power that competes with synthetic antioxidants. XTT assay results demonstrated that although both extracts displayed a considerable cytotoxicity against the tested cancer cell lines in a time and dose-dependent manner, ME expressed its selective inhibitory action towards MCF-7 cells with an IC50 value of 20.4 μg/mL for 72 h. These results may serve as a basis for further in vivo studies to examine the potential applications of T. erzincanense in food and pharmaceutical industries.

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• MakaleYalnız künye2021/r/gumushane/12999
  Determination of Angelica archangelica’s Antioxidant Capacity and Mineral Content

  Topal, Meryem; Ozturk Sarıkaya, S. Beyza; Topal, Fevzi

  The aim of this study was to determine the mineral contents by ICP-MS and the antioxidant capacity of Angelica archangelica plant, which is not well known in the literature. For this purpose, ethyl alcohol extracts of the branches and leaves of the plant; different bioanalytical methods such as reducing capacity by Fe3+−Fe2+ transformation method, Fe3+-TPTZ reducing capacity by FRAP method, Cu2+-Cu+ reducing capacity by CUPRAC method, the ferric ions (Fe2+) chelating activity by using bipyridyl reagent, DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, DMPD (N,N-dimethyl-p-phenylenediamine), ABTS (2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging activities, total antioxidant activity determination according to thiocyanate method, total phenolic and total flavonoid activity were used. As standard, BHT, BHA, α-Tocopherol, Trolox were used. A. archangelica branches and leaves demonstrated 88.13 % and 93.90 % inhibition of linoleic acid emulsion peroxidation at 20 mg mL−1 concentration. Also, it was determined that both leaves and branches of A. archangelica plant were dense with P, Mg, Ca and K minerals with ICP-MS device. It is considered that the results of the manuscript will guide the antioxidant studies, medicine, cosmetics, pharmacology and food sector.

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• Derleme MakaleAçık Erişim2019/r/gumushane/14828
  The importance of antioxidants and place in today’s scientific and technological studies

  Zehiroglu, Cuma; Ozturk Sarikaya, Sevim Beyza

  Antioxidants have become scientifically interesting compounds due to their many benefits such as anti-aging and anti-inflammatory. Today, it is still used in many areas. In food technology, antioxidants are added to many foodstuffs in order to enrich the foods and eliminate the problems. Therefore, studies to determine the antioxidant activities of natural foods and their components are also continuing rapidly. Antioxidants have also been replaced in the encapsulation studies used for the preservation and stabilization of food components. Of course, preservation of foods is as important as their production. The latest packaging techniques for food preservation are edible films and coatings. The protective function of edible films and coatings can be improved by the addition of antioxidants. Unlike these, studies on plants and animals have been investigated in vivo in terms of how the antioxidant activity changes as a result of metabolic activities. The role of antioxidant enzymes in these studies is quite large. Many results have been found for the elimination of diseases by either in vivo or in vitro studies regarding antioxidants. Thus, the importance of antioxidants increased with the use in pharmacology, cosmetics and medicine. In this study, we tried to bring a current perspective to antioxidants played an active role in many fields by combining the technological applications and scientific studies of antioxidants. In order to further customize the issue, we have done this especially for the food and health field and we have tried to emphasize the importance of antioxidants in this way.

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• MakaleAçık Erişim2018/r/gumushane/15318
  The effect of apple cider vinegar and mushroom stalk supplementation on laying hens

  Karaalp, Musa; Aksakal, Vecihi; Sarikaya, Sevim Beyza Ozturk; Urusan, Hilal; Bayram, Bahri; Zulkadir, Ayhan

  The aim of this trial was to study the effects of consuming water with apple cider vinegar (ACV) and feed including mushroom (Agaricus bisporus) stalk (MS) on performance and certain egg characteristics. The hens were separated into four treatment groups (with 4 replicates and 6 hens per replicate). The experimental groups were control (not consumed ACV and MS), ACV (3 ml/l drinking water), MS (20 g/kg feed) and ACV+MS (combination). At the end of the experiment, among the performance criteria tested, body weight, feed intake, egg production and weight of eggs, and feed efficiency were not affected by the treatments (P>0.05). Treatments had no effect on physical external and internal egg characteristics. The amount of yolk malondialdehyde of the ACV and MS groups was lower than the control group (P<0.05) in stored eggs (28 d) (P<0.05).

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• MakaleAçık Erişim2015/r/gumushane/15760
  Acethylcholinesterase inhibitory potential and antioxidant properties of pyrogallol

  Ozturk Sarikaya, S. Beyza

  Pyrogallol is found naturally in crops and fruits of many plants. It is also an active ingredient of many pharmaceuticals. For this reason, we employed different in vitro antioxidant assays such as cupric ion Cu2+ reducing power, Fe3+ reducing power, total antioxidant activity by ferric thiocyanate method, ABTS radical scavenging, DMPD radical scavenging, DPPH • scavenging, Fe2+ chelating, O2•- scavenging and H2O2 scavenging activities of pyrogallol. Pyrogallol inhibited 77.95% lipid peroxidation of linoleic acid emulsion at 30 μg/mL concentration. BHA, BHT, α-tocopherol and trolox exhibited inhibitions of 89.88, 89.97, 83.82 and 91.85% against peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, pyrogallol was an effective of all the scavenging and reducing power results. In this study, pyrogallol was also evaluated as potential inhibitor for acethycholinesterse enzyme. The results showed that pyrogallol exhibited potent acetylcholinesteras inhibitory activity with IC50 and Ki values 10.2 and 8.6 μM, respectively.

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