İlgi alanları

• MakaleAçık Erişim2024/r/gumushane/10442
  Comparative Cranial Geometric Morphometrics among Wistar Albino, Sprague Dawley, and WAG/Rij Rat Strains

  Agaç, Duygu Kucuk; Onuk, Burcu; Gündemir, Ozan; Kabak, Murat; Manuta, Nicoleta; Çakar, Buket; Janeczek, Maciej; Crampton, Denise Amber; Szara, Tomasz

  Simple Summary Geometric morphometrics allows the examination of the shape variation of structures. In this study, the skull, mandible, and teeth of three different strains of rats were examined. The results showed that the strains significantly differed in shape. The most important shape difference in the skull was the variation of the cranium from an oval to an elongated structure. In this respect, Sprague Dawley rats showed a more elongated skull, while WAG/Rij rats had a more oval skull. Wistar Albino rats showed a more moderate shape variation. WAG/Rij rats showed very different shapes of the mandible and teeth compared to the other strains. But statistically, it was seen that all strains were completely separated from each other.Abstract This research utilizes geometric morphometrics to investigate shape variation in the skull, mandible, and teeth among three rat strains: Wistar Albino (WA), Sprague Dawley (SD), and WAG/Rij (WR). Through the analysis of 48 rats using 2D geometric morphometric techniques, significant differences in their skull morphology were identified. This study indicates a shift from a rectangular to an oval cranial shape across strains, with notable size and morphological variances. Particularly, the WR strain's skull shape significantly differs from the SD and WA strains, suggesting distinct ecological or genetic pathways. Compared to the skull, mandible shape differences are less pronounced, but still significant. The WR strain exhibits a distinct mandible shape, potentially reflecting ecological adaptations like dietary habits. The teeth shape of WR rats is the most distinct. SD rats consistently exhibited larger sizes in both skull and mandible measurements, while WR rats were notably smaller. Interestingly, sexual dimorphism was not statistically significant in skull and teeth sizes, aligning with findings from previous studies. However, the mandible showed clear size differences between sexes, underscoring its potential for adaptive or behavioral studies. In summary, this study provides a comprehensive analysis of morphological variations in rat strains, highlighting the intricate interplay of size, shape, and ecological factors. These findings lay a foundation for deeper explorations into the adaptive, ecological, or genetic narratives influencing rat morphology.

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• MakaleAçık Erişim2024/r/gumushane/19971
  Kızıl Tilkide (Vulpes vulpes) Sternum’un Morfolojik İncelenmesi

  Onuk, Burcu; Selviler-Sizer, Sedef; Ağaç, Duygu Küçük

  Sternum, göğüs iskeletinin ventral bölümünü oluşturmak üzere, cartilago costalis’ler aracılığıyla costa’lara bağlanan bir kemiktir. Hayvanlarda kemiklerin şekli ve yapısında türler arasında farklılıklar bulunmaktadır. Bu nedenle farklı hayvan türlerinde kemiklerin anatomik yapılarının makroskobik ve morfometrik olarak bilinmesi klinik uygulamalar ve hastalıkların teşhisi açısından önemlidir. Bu çalışma ile ilk kez kızıl tilki sternum’unun morfolojik ve morfometrik özelliklerinin belirlenmesi amaçlandı. Bu amaçla çalışmada 4 adet kızıl tilki sternum’u kullanıldı. Toplam 8 adet sternebradan oluşan sternum, manubrium sterni, corpus sterni ve proc. xiphoideus’tan oluşmaktaydı ve ortalama uzunluğu 16,2±05 cm idi. En cranial’deki parçayı oluşturan manubrium sterni kısa silindir görünümündeydi ve hafif yukarı doğru bir duruşa sahipti. Orta kısmına doğru costa’ların bağlanması için genişleme mevcuttu. Corpus sterni’yi oluşturan sternebra’ların şekli silindir biçimindeydi ve boyları birinciden altıncı srternebra’ya doğru kısalarak devam etmekteydi. Yatay olarak geniş, dikey olarak ince bir yapıya sahip olan proc. xiphoideus’un üzerinde costa bağlantısı mevcut değildi ve en ucunda yaprak şeklinde bir cartilago xiphoidea’nın olduğu görüldü. Sonuç olarak yapılan bu çalışma ile kızıl tilki sternum’una ait morfometrik veriler ilk kez elde edildi. Kızıl tilki sternum’unun makroanatomik yapısı genel itibariyle carnivor’lara benzer görüldü.

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• MakaleAçık Erişim2024/r/gumushane/19883
  Comparative Macroscopic and Morphometric Analysis of Mandible in Different Mouse (Balb-c; Cd-1; C57bl/6) and Rat Strains (Wistar Albino; Sprague Dawley; Wag/Rij)

  Onuk, Burcu; Ağaç, Duygu Küçük

  Since the morphology of the mandible varies among animal species, it is the subject of many studies. The aim of this study was to investigate the macroanatomical and morphometric aspects of the mandible in three different rat (Sp-ague Dawley, Wistar Albino, Wag/Rij) and mouse strains (Balb-c, C57bl/6, CD-1). In this study, which was conducted using a total of 96 mandibles, 8 females and 8 males from each strain, measurements were taken from 6 parameters.Similarities and differences between strains and sexes were revealed by statistical analyses. The mandibles were examined under a stereomicroscope. For Balb-c, the termination of proc. angularis at the level of proc. condylaris and the shallow tuberculum masseterica were identified as distinctive anatomical structures; for Wag/Rij, the termination of proc. angularis at the level of proc. condylaris, the prominent tuberculum masseterica, and the localization of the molar teeth were macroscopically revealed as distinctive features. Specific measurement parameters were proc. coronoideus height for Balb-c; mandible length and lower M3 crown length for CD-1, while specific measurement point was no available for C57bl/6. In rats, proc. coronoideus height was the specific measurement point for Wag/ Rij; lower M3 crown length was decisive for Sprague Dawley. The mandible length parameter was specific for all rat strains. It was concluded that strain determination could be made by looking at these distinctive parameters.

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• MakaleAçık Erişim2024/r/gumushane/13923
  Shape variation in cranium, mandible and teeth in selected mouse strains

  Ağaç, Duygu Küçük; Oktay, Ece; Onuk, Burcu; Kabak, Murat; Gündemir, Ozan

  There are different strains of laboratory mouse used in many different fields. These strains differ anatomically. In order to determine these anatomical differences, shape analysis was conducted according to species. CD-1, C57bl/6 and Balb-c strains were preferred to study these differences. Forty-eight adult mouse strains belonging to these strains were utilized. The bones were photographed and geometric morphometry was applied to these photographs. Principal Component Analysis was applied to determine shape variations. In Principal component 1 for cranium, CD-1 and C57bl/6 strain groups showed different shape variations, while Balb-c strain group showed similar shape variations to the other strain groups. Principal Component 1 for the mandible separated the CD-1 and C57bl/6 strain groups in terms of shape variation. Principal Component 2 explained most of the variation between the C57bl/6 and CD-1 lineage groups. In PC1 for molars, the CD-1 group showed a different shape variation from the other groups. Mahalanobis distances and Procrustes distances were measured using Canonical variance analysis to explain the differences between the lineage groups. These measurements were statistically significant. For cranium, in canonical variate 1, CD-1 group of mouse and Balb-c group of mouse were separated from each other. In canonical variate 2, C57bl/6 group of mouse were separated from the other groups. For mandible, Balb-c group of mouse in canonical variate 1 and CD-1 group of mouse in canonical variate 2 were separated from the other groups. For molars, CD-1 group of mouse in canonical variate 1 and Balb-c group of mouse in canonical variate 2 were separated from the other groups. It was thought that these anatomical differences could be caused by genotypic factors as well as dietary differences and many different habits that would affect the way their muscles work.

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