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13 →- MakaleYalnız künye2026Improving conservation breeding: a molecular approach to sex determination in Northern bald ibises (G. eremita)
Yi̇ği̇n, Akin; Bozkaya, Faruk; Gürler, Şükrü; Yılmaz, Bestami; Demi̇rci̇oğlu, İsmai̇l; Yilmaz, Rahşan; Ekti̇ren, Reşat; Ekici, Seda
Introduction: Bald ibises (G. eremita) are migratory birds that have been listed as near extinction by the International Union for Conservation of Nature since 1994 due to a dramatic decline in population size. As this species does not exhibit sexual dimorphism, reliable sex determination requires the use of molecular genetic techniques. The aim of this study was to establish an effective and practical molecular method for sex identification in bald ibises. Materials and methods: The study was conducted on blood and feather samples collected from 96 bald ibises housed at the Şanlıurfa Birecik Bald Ibis Breeding Station. The chromodomain-helicase-DNA-binding (CHD) gene region was amplified by polymerase chain reaction (PCR) using the primer pairs 2550 F–2718R, 1237–1272 H, and P2–P8. PCR products were analyzed by agarose gel electrophoresis. Amplicons obtained with the P2–P8 primer pair were further subjected to restriction fragment length polymorphism (RFLP) analysis using the restriction enzyme HaeIII. In addition, high-resolution melting (HRM) analysis was performed using real-time PCR with primers 1237–1272 H. Results: Following HaeIII digestion of PCR products generated with the P2–P8 primer pair, male individuals exhibited a single band of approximately 350 bp, whereas female individuals showed two bands of approximately 350 and 400 bp. HRM analysis also successfully distinguished sexes based on distinct melting profiles. Both blood- and feather-derived DNA samples were suitable for PCR amplification and subsequent analyses. Conclusion: The results demonstrate that PCR–RFLP analysis using the P2–P8 primer pair combined with HaeIII digestion is a reliable method for sex determination in bald ibises. Furthermore, HRM analysis was applied for the first time in G. eremita and proved to be an effective alternative approach. These molecular methods provide practical and accurate tools for sex identification in conservation and breeding programs of this endangered species.
- MakaleAçık Erişim2025Characterization of the complete mitogenomic and phylogenetic of the Columba livia breed Şebap pigeon
Çelik, Romedi; Bilgen, Nüket; Yigin, Akın; Gürler, Şükrü; Bozkaya, Faruk; Kutlu, Furkan; Akkurt, Mustafa Yenal
The initial phase of the studies focused on the conservation of gene resources involves the phenotypic and genetic characterization of existing breeds. By elucidating the differences at the mitochondrial DNA (mtDNA) level, a particularly suitable source for genetic analyses, it becomes feasible to calculate genetic distances between local breeds, identify potential domestication centers through evolutionary analyses, and construct phylogeny trees depicting kinship relationships between among breeds. Şanlıurfa holds significance in pigeon breeding due to its prolonged history of traditional pigeon breeding, establishing itself as one of the pigeon domestication centers. This study aims to genetically characterize Şebap pigeon breed which is specific to Şanlıurfa province in Türkiye. The mitogenome of the Şebap pigeon was analyzed by next generation sequencing and determined to be 17,232 bp in size, comprising 13 Protein-Coding Genes (PCGs), two Ribosomal RNAs (rRNAs), 22 Transfer RNAs (tRNAs). The overall mitogenome composition includes 29.6% for A, 24.5% for T, 31.2% for C, and 14.7% for G, with a notable A–T richness of 54.1%. Notably, ND6 is encoded on the L strand while the others are encoded on the H strand. Our study has provided a comprehensive examination of whole mitochondrial DNA. Using this information, we have constructed a phylogenetic tree, locating the Şebap pigeon within the rock pigeon lineage.
- MakaleAçık Erişim2024Comparative Cranial Geometric Morphometrics among Wistar Albino, Sprague Dawley, and WAG/Rij Rat Strains
Agaç, Duygu Kucuk; Onuk, Burcu; Gündemir, Ozan; Kabak, Murat; Manuta, Nicoleta; Çakar, Buket; Janeczek, Maciej; Crampton, Denise Amber; Szara, Tomasz
Simple Summary Geometric morphometrics allows the examination of the shape variation of structures. In this study, the skull, mandible, and teeth of three different strains of rats were examined. The results showed that the strains significantly differed in shape. The most important shape difference in the skull was the variation of the cranium from an oval to an elongated structure. In this respect, Sprague Dawley rats showed a more elongated skull, while WAG/Rij rats had a more oval skull. Wistar Albino rats showed a more moderate shape variation. WAG/Rij rats showed very different shapes of the mandible and teeth compared to the other strains. But statistically, it was seen that all strains were completely separated from each other.Abstract This research utilizes geometric morphometrics to investigate shape variation in the skull, mandible, and teeth among three rat strains: Wistar Albino (WA), Sprague Dawley (SD), and WAG/Rij (WR). Through the analysis of 48 rats using 2D geometric morphometric techniques, significant differences in their skull morphology were identified. This study indicates a shift from a rectangular to an oval cranial shape across strains, with notable size and morphological variances. Particularly, the WR strain's skull shape significantly differs from the SD and WA strains, suggesting distinct ecological or genetic pathways. Compared to the skull, mandible shape differences are less pronounced, but still significant. The WR strain exhibits a distinct mandible shape, potentially reflecting ecological adaptations like dietary habits. The teeth shape of WR rats is the most distinct. SD rats consistently exhibited larger sizes in both skull and mandible measurements, while WR rats were notably smaller. Interestingly, sexual dimorphism was not statistically significant in skull and teeth sizes, aligning with findings from previous studies. However, the mandible showed clear size differences between sexes, underscoring its potential for adaptive or behavioral studies. In summary, this study provides a comprehensive analysis of morphological variations in rat strains, highlighting the intricate interplay of size, shape, and ecological factors. These findings lay a foundation for deeper explorations into the adaptive, ecological, or genetic narratives influencing rat morphology.
- MakaleAçık Erişim2024Kızıl Tilkide (Vulpes vulpes) Sternum’un Morfolojik İncelenmesi
Onuk, Burcu; Selviler-Sizer, Sedef; Ağaç, Duygu Küçük
Sternum, göğüs iskeletinin ventral bölümünü oluşturmak üzere, cartilago costalis’ler aracılığıyla costa’lara bağlanan bir kemiktir. Hayvanlarda kemiklerin şekli ve yapısında türler arasında farklılıklar bulunmaktadır. Bu nedenle farklı hayvan türlerinde kemiklerin anatomik yapılarının makroskobik ve morfometrik olarak bilinmesi klinik uygulamalar ve hastalıkların teşhisi açısından önemlidir. Bu çalışma ile ilk kez kızıl tilki sternum’unun morfolojik ve morfometrik özelliklerinin belirlenmesi amaçlandı. Bu amaçla çalışmada 4 adet kızıl tilki sternum’u kullanıldı. Toplam 8 adet sternebradan oluşan sternum, manubrium sterni, corpus sterni ve proc. xiphoideus’tan oluşmaktaydı ve ortalama uzunluğu 16,2±05 cm idi. En cranial’deki parçayı oluşturan manubrium sterni kısa silindir görünümündeydi ve hafif yukarı doğru bir duruşa sahipti. Orta kısmına doğru costa’ların bağlanması için genişleme mevcuttu. Corpus sterni’yi oluşturan sternebra’ların şekli silindir biçimindeydi ve boyları birinciden altıncı srternebra’ya doğru kısalarak devam etmekteydi. Yatay olarak geniş, dikey olarak ince bir yapıya sahip olan proc. xiphoideus’un üzerinde costa bağlantısı mevcut değildi ve en ucunda yaprak şeklinde bir cartilago xiphoidea’nın olduğu görüldü. Sonuç olarak yapılan bu çalışma ile kızıl tilki sternum’una ait morfometrik veriler ilk kez elde edildi. Kızıl tilki sternum’unun makroanatomik yapısı genel itibariyle carnivor’lara benzer görüldü.
- MakaleAçık Erişim2024Comparative Macroscopic and Morphometric Analysis of Mandible in Different Mouse (Balb-c; Cd-1; C57bl/6) and Rat Strains (Wistar Albino; Sprague Dawley; Wag/Rij)
Onuk, Burcu; Ağaç, Duygu Küçük
Since the morphology of the mandible varies among animal species, it is the subject of many studies. The aim of this study was to investigate the macroanatomical and morphometric aspects of the mandible in three different rat (Sp-ague Dawley, Wistar Albino, Wag/Rij) and mouse strains (Balb-c, C57bl/6, CD-1). In this study, which was conducted using a total of 96 mandibles, 8 females and 8 males from each strain, measurements were taken from 6 parameters.Similarities and differences between strains and sexes were revealed by statistical analyses. The mandibles were examined under a stereomicroscope. For Balb-c, the termination of proc. angularis at the level of proc. condylaris and the shallow tuberculum masseterica were identified as distinctive anatomical structures; for Wag/Rij, the termination of proc. angularis at the level of proc. condylaris, the prominent tuberculum masseterica, and the localization of the molar teeth were macroscopically revealed as distinctive features. Specific measurement parameters were proc. coronoideus height for Balb-c; mandible length and lower M3 crown length for CD-1, while specific measurement point was no available for C57bl/6. In rats, proc. coronoideus height was the specific measurement point for Wag/ Rij; lower M3 crown length was decisive for Sprague Dawley. The mandible length parameter was specific for all rat strains. It was concluded that strain determination could be made by looking at these distinctive parameters.
- MakaleAçık Erişim2024Shape variation in cranium, mandible and teeth in selected mouse strains
Ağaç, Duygu Küçük; Oktay, Ece; Onuk, Burcu; Kabak, Murat; Gündemir, Ozan
There are different strains of laboratory mouse used in many different fields. These strains differ anatomically. In order to determine these anatomical differences, shape analysis was conducted according to species. CD-1, C57bl/6 and Balb-c strains were preferred to study these differences. Forty-eight adult mouse strains belonging to these strains were utilized. The bones were photographed and geometric morphometry was applied to these photographs. Principal Component Analysis was applied to determine shape variations. In Principal component 1 for cranium, CD-1 and C57bl/6 strain groups showed different shape variations, while Balb-c strain group showed similar shape variations to the other strain groups. Principal Component 1 for the mandible separated the CD-1 and C57bl/6 strain groups in terms of shape variation. Principal Component 2 explained most of the variation between the C57bl/6 and CD-1 lineage groups. In PC1 for molars, the CD-1 group showed a different shape variation from the other groups. Mahalanobis distances and Procrustes distances were measured using Canonical variance analysis to explain the differences between the lineage groups. These measurements were statistically significant. For cranium, in canonical variate 1, CD-1 group of mouse and Balb-c group of mouse were separated from each other. In canonical variate 2, C57bl/6 group of mouse were separated from the other groups. For mandible, Balb-c group of mouse in canonical variate 1 and CD-1 group of mouse in canonical variate 2 were separated from the other groups. For molars, CD-1 group of mouse in canonical variate 1 and Balb-c group of mouse in canonical variate 2 were separated from the other groups. It was thought that these anatomical differences could be caused by genotypic factors as well as dietary differences and many different habits that would affect the way their muscles work.
- MakaleAçık Erişim2020The impact of dietary tarragon (Artemisia dracunculus) on serum apelin, brain-derived neurotrophic factor, cardiac troponin concentrations and histopathology of liver tissue in laying hens housed at different stocking densities
Bayraktar, Bulent; Tekce, Emre; Kaya, Hacer; Karaalp, Musa; Turunc, Eylul
Due to its association with several other stress factors (poultry house gases, inadequate ventilation, heat, cold and poor hygiene), the high stocking density is a major stress factor that adversely affects the health and performance of poultry and the quality of the poultry products. Therefore, this experimental study was aimed at analysing the impact of different doses of dietary tarragon (Artemisia dracunculus) on the serum apelin, plasma brain-derived neurotrophic factor (p-BDNF), and cardiac troponin I (cTnI) concentrations, and the correlation between these indicators in laying hens housed at different stocking densities. The aim of this study is to investigate the effects of adding tarragon in different ratios to laying hen rations in the 2ndovulation period on the cTnI, apelin, and BDNF hormone concentrations and the liver histopathology. The experiment was carried out over a period of eight weeks, with 192 Lohman Brown commercial hybrids at 50 weeks of age. Eight groups (four replicates each), composed of laying hens of equal body weight, which were housed at stocking densities of 580 cm2/hen and 810 cm2/hen and received 0, 1, 5 and 10 mmol/kg of tarragon (Artemisia dracunculus) in the feed, were established. At the end of the trial, 96 of the housed egg-laying hens (3 birds in each subgroup, a total of 12 birds in each group) were randomly selected and blood samples were taken from the vena subcutanea ulnaris. The samples collected were analysed for the apelin, p-BDNF, and cTnI contents. The analysis results demonstrated that tarragon supplementation had no effect on the serum apelin, p-BDNF and cTnI concentrations (P > 0.05). The Sub-Groups ST1, ST1.2, and ST6 presented with severe hyperaemia of the sinusoidal, portal and acinar blood vessels, whilst the hyperaemia of these blood vessels was moderate in Sub-Group ST12. Apelin, BDNF, and cTnI can act as protective factors against negative consequences of stress (e.g., stocking density or heat stress).
- MakaleAçık Erişim2020Influence of lactobacillus reuteri on internal organ weight, performance and meat quality of Japanese quail (Coturnixcoturnix japonica) under heat stress; [Einfluss von lactobacillus reuteri auf das gewicht der inneren organe, die leistung und die fleischqualität Japanischer Wachteln (Coturnix coturnix japonica) unter Hitzestress]
Tekce, E.; Bayraktar, B.; Aksakal, V.; Dertli, E.; Kamiloğlu, A.; Çinar Topçu, K.; Kaya, H.; Takma, C.; Yasulergezer, N.
In the present study, the effects of Lactobacillus reuteri E81 (LRE) supplemented at different doses (200, 400 and 600 mg/kg) into the diets on performance, meat quality and internal organ weights of Japanese quail (Coturnixcoturnix japonica) were investigated under heat stress (HS) conditions (37°C). For the experiments, 448 one-day old quails were used. Seven days of training (physical exercise) and a 35-day fattening period were applied. On day 7 of the trial, 8 groups [4 under control temperature of 25°C (CT): control (C), LRE-200, LRE-400 and LRE-600 and 4 under HS: control (HSC), SLRE-200, SLRE-400 and SLRE-600] were formed, each group consisting of four replicates with 14 quail each. At the end of this study, the CT group fed 400 mg/kg LRE showed the best performance results, whereas no effect was found for groups under HS in comparison to the control group (HSC). Samples taken on the 21st and 42nd day revealed that addition of probiotics in the CT and HS groups induced no effect on weight of the internal organs (p > 0.05). Analysis of meat quality on the 21st day showed that LRE caused an increase in pH in both the HS and CT groups. Further, 400-mg/kg LRE supplementation induced an increase in thiobarbituric acid reactive substances (TBARS) in the group under HS. In the CT groups on day 42 no effect of LRE was found on colour parameters or on the TBARS value, except for a decrease in the pH value in the LRE supplemented groups compared with the control. In HS groups a decrease in the L* value and an increase in the TBARS value was found compared to CT groups (p < 0.05), but there was no effect on other colour parameters or pH value. In conclusion, in the present study supplementation of different doses of LRE E81 to quail diets had no effect on performance, internal organ weight or meat quality under HS.
- MakaleAçık Erişim2020Response of Japanese quails (Coturnix coturnix japonica) to dietary inclusion of Moringa oleifera essential oil under heat stress condition
Tekce, Emre; Bayraktar, Bülent; Aksakal, Vecihi; Dertli, Enes; Kamiloğlu, Aybike; Çinar Topcu, Kübra; Takma, Çiğdem; Gül, Mehmet; Kaya, Hacer
The present study was aimed at investigating the effects of the dietary use of different doses of Moringa oleifera essential oil (MOEO) on performance parameters, internal organ weights and meat quality in Japanese quails (Coturnix coturnix japonica) exposed to Heat Stress (HS). For this purpose, 320 male day-old quail chicks were used. Seven days of preliminary (physical exercise) and a 35-day fattening period were applied. The animals were divided into 8 groups, each including 40 animals according to temparature (C: 25 °C and SC: 37 °C) and dietary doses (C, 200, 400 and 600). Each group was divided into 4 subgroups, each comprising 10 animals. The consequences of this study showed that, in the stress-free (HSF) groups, while all of the different doses of dietary MOEO positively affected growth performance, feed intake and feed efficiency, in the HS groups, similar positive effects were observed with the use of 200 ppm of dietary MOEO. While no effect was detected on internal organ weights on Days 21 and 42 in the HSF and HS groups, a dose-dependent increase was determined in the intestinal pH value on day 42 in the HS group (p <.05). Additionally, in the analysis of meat quality on the 21st and 42 days compared to the controls, no effect was observed on the meat colour parameters value and TBARS level in the HS and HSF groups. Based on the consequences of this study, it is considered that, MOEO could be used as an alternative product in poultry, at a dose of 200 ppm, to reduce the detrimental effects of HS.HIGHLIGHTS Temperature stress, performance parameters and serious problems in the health of poultry. Moringa olifera helps regulate performance parameters in poultry exposed to heat stress. Since the addition of Moringa oleifera to the diets does not affect the organ weight and meat quality in 21 and 42 days, it does not cause any negative effects for the consumer.
- MakaleAçık Erişim2020Effects of lactobacillus reuteri e81 added into rations of chukar partridges (Alectoris chukar) fed under heat stress conditions on fattening performance and meat quality
Tekce, Emre; Bayraktar, B.; Aksakal, V.; Dertli, E.; Kamiloğlu, A.; Çinar, K.; Takma, Ç.; Kaya, H.; Gül, M.
This study investigated the effects of the addition of Lactobacillus reuteri E81 (LRE) into rations of chukar partridges (Alectoris chukar) fed under heat stress (HS) conditions on fattening performance and meat quality. This study included 256 chukar partridges aged 1 day. The study comprised an adaptation period of 7 days and a fattening period of 35 days and included 8 different groups with 32 animals in each group. Each group was further divided into four subgroups with eight animals in each subgroup. At the end of the study, the best results in terms of fattening performance in the non-HS groups were obtained in the LRE 600 ppm group and in the HS groups, SLRE 200 ppm had the best effect on average live weight and average live weight increase, whereas SLRE 400 ppm had the best effect on FCR (p<0.05). The analysis of the samples collected from chukar partridges on day 21 showed that, there was no effect on the colour parameters and Thiobarbituric acid reactive substances (TBARS) level in the LRE in the HS and non-HS groups, whereas the meat pH level decreased in the SLRE 400 ppm group (p<0.05). The analysis of the samples collected on day 42 showed that there was no effect on colour parameters in the HS and non-HS groups. TBARS level decreased at the dose of LRE 200 ppm in the non-HS group, and the meat pH level decreased in both HS and non-HS groups (p<0.05).
Tam Kayıt
| Alan | Değer | Dil |
|---|---|---|
| dc.date.accessioned | 2026-10-22T08:47:36.341327157Z | |
| dc.date.available | 2026-10-22T08:47:36.341327157Z | |
| dc.identifier.uri | https://rdlopen.rawdatalibrary.net/r/gumushane/10145 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.title | Veterinerlik Bölümü | tr |
| dc.title | Department of Veterinary | en |
| dspace.entity.type | OrgUnit | |
| organization.identifier.code | gumushane-siran-mustafa-beyaz-veterinerlik | |
| organization.unitType | department | |
| rdl.unit.alias | \bveterinerlik bolumu\b | |
| rdl.unit.alias | \bveterinary\b |