İlgi alanları

Sürdürülebilir Kalkınma Amaçları

• 9
• 1

• MakaleAçık Erişim2026/r/gumushane/13546
  Chemical Profiling of Stachys cretica subsp. anatolica Rech. f. (Endemic) Essential Oils and their Methanol Extracts with Evaluation as Enzyme Inhibitors, Antioxidant, and Antimicrobial Agents

  Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman; Aygül, İmdat

  Enzyme inhibition activities, phenolic compounds, antioxidant activities, bioactive compounds, antimicrobial activities, and chemical components of essential oil and methanol extracts obtained from the aerial parts of S. cretica subsp. anatolica were investigated. The main phenolic compounds of aerial parts were catechin, oleuropein, and epicatechin. The determined enzyme inhibitor activities highlight the potential of S. cretica subsp. anatolica as a source of bioactive compounds, particularly for carbonic anhydrase and cholinesterase inhibition. The essential oil and methanol extract exhibited remarkable activities against CA-II, AChE, and BChE, although they were less potent than standard inhibitors. The essential oils generally showed stronger antimicrobial activity compared to the 30% methanol extracts across most bacterial and fungal strains, as evidenced by minimum lethal concentration (MLC) and lower minimum inhibitory concentration (MIC) values and larger inhibition zones. Chloramphenicol used alone exhibited the highest antimicrobial efficacy, with the lowest MIC and MLC values and the largest inhibition zones. The essential oils of S. cretica subsp. anatolica were determined as esters, oxygenated sesquiterpenes, and aldehydes in aerial parts. The main components were found to be hexahydrofarnesyl acetone in the aerial parts.

  İncele
• MakaleAçık Erişim2026/r/gumushane/12153
  Chemical Profiling of Essential Oils and Evaluation of Enzyme Inhibition, Antioxidant, and Antimicrobial Activities in Both Methanol Extract and Essential Oils of Centaurea oltensis

  Aygül, İmdat; Akşit, Berfin

  The phytochemical composition and biological activities of Centaurea oltensis, an endemic species in Türkiye, were studied. GC–MS/FID analysis identified 128 compounds representing 99.2% of the EO, with decanoic acid (15.1%), α-pinene (7.9%), and sabinyl acetate (5.4%) as the principal constituents. GC–MS/FID analysis identified 128 compounds representing 99.2% of the essential oil (EO), with decanoic acid (15.1%), α-pinene (7.9%), and sabinyl acetate (5.4%) as the principal constituents. LC–MS/MS analysis of the methanol extract (ME) revealed high concentrations of ferulic acid (2410 µg/g dw) and chlorogenic acid (1170 µg/g dw), together with caffeic, salicylic, and 4-hydroxybenzoic acids. The ME exhibited potent carbonic anhydrase-II inhibition (IC₅₀ = 0.028 µg/mL) and significant α-glucosidase inhibition (IC₅₀ = 0.255 mg/mL). The EO showed strong acetylcholinesterase and butyrylcholinesterase inhibitory activities, with IC₅₀ values of 0.0145 µg/mL and 0.204 µg/mL, respectively. Antioxidant assays, including DPPH, ABTS, and FRAP, demonstrated superior activity of the ME, correlating with its higher phenolic and flavonoid contents. The EO displayed remarkable antibacterial activity against Staphylococcus aureus (MIC = 0.02 mg/mL) and moderate antifungal activity against Candida albicans. Overall, C. oltensis demonstrated considerable chemical diversity and promising antioxidant, antimicrobial, neuroprotective, and enzyme inhibitory potential for future pharmacological and therapeutic applications.

  İncele
• MakaleAçık Erişim2026/r/gumushane/12128
  Chemical Profiling and Biological Activities of Methanol Extracts and Essential Oils From Ferulago Platycarpa Boiss. & Balansa (Endemic): Enzyme Inhibition, Antioxidant, and Antimicrobial Properties

  Aygül, İmdat; Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman

  This study aimed to evaluate the phytochemical composition and biological activities of Ferulago platycarpa methanol extract (ME) and essential oil (EO). The EO was obtained via hydrodistillation and analyzed by GC-MS, revealing sesquiterpene hydrocarbons as the dominant class, with caryophyllene (65.8%) and α-pinene (9.65%) as the major constituents. The methanol extract was subjected to LC-MS/MS analysis, which identified eleven phenolic compounds, with chlorogenic acid as the most abundant. The extract showed significantly higher total phenolic (1196.22±11.64 mg GAE/100g) and antioxidant (1870.00±17.69 mg QEE/100g) contents compared to the ME. In vitro enzyme inhibition assays demonstrated that the methanol extract exhibited potent inhibitory activity against carbonic anhydrase II (CA-II, ICSO= 0.023 pg/mL), acetylcholinesterase (AChE, ICSO= 110 pg/mL), and butyrylcholinesterase (BChE, IC50= 390 pg/mL). In contrast, the EO showed higher inhibition against α-amylase (IC50= 5920±10.45 pg/mL) and BChE (IC50= 1.32±0.65 pg/mL), while its α-glucosidase showed no inhibition. Antioxidant assays indicated superior activity for the methanol extract compared to the EO. Furthermore, antimicrobial testing revealed that the EO demonstrated broader and more effective antimicrobial action, exhibiting lower MIC and MBC values against several bacterial and fungal strains. Collectively, these results highlight F. platycarpa as a valuable source of bioactive compounds with promising applications in antidiabetic, neuroprotective, antioxidant, and antimicrobial therapies.

  • 
  İncele
• MakaleAçık Erişim2025/r/gumushane/19723
  Evaluation of antioxidant, antimicrobial, and α-amylase enzyme inhibition properties of Rosa canina seed extracts prepared with different solvents using maceration and ultrasound-assisted extraction methods

  Baltaci, Cemalettin; Akmeşe, Osman; Aygül, İmdat; Kılınç, Kagan; Hafedh, Mohammed

  The efficient extraction of bioactive molecules from medicinal plants is strongly related to the solvents and methods used. This study evaluated the impact of solvent selection and extraction methods on the bioactive potential of Rosa canina seed extracts, focusing on antioxidant, antimicrobial, and α-amylase inhibitory activities. Four solvents —dimethyl sulfoxide (DMSO), ethanol-water (80:20), ethyl acetate, and acetonitrile— were paired with maceration and ultrasonic techniques to generate eight extracts (DMM, DMU, ESM, ESU, EAM, EAU, ACM, ACS). Antioxidant activity was assessed via total phenolic (TPC) and flavonoid (TFC) content, FRAP, DPPH, and ABTS assays. Antimicrobial efficacy was tested against seven microorganisms (Gram+, Gram−, and yeast) using well diffusion and MIC methods, while α-amylase inhibition was quantified via IC50. The ethyl acetate ultrasonic extract (EAU) exhibited the highest TPC (63.86 mg GAE/g) and TFC (210.23 mg QE/g), along with superior antioxidant activity across all assays (P < 0.05). Ethyl acetate maceration (EAM) ranked second, underscoring ethyl acetate’s efficacy. Acetonitrile maceration (ACM) demonstrated the strongest α-amylase inhibition (IC50 = 20.46 mg/mL). All extracts showed notable antimicrobial activity against Aeromonas hydrophila and Klebsiella pneumoniae, though efficacy varied for other strains. DMSO-based extracts (DMM/DMU) consistently underperformed. These findings highlight ethyl acetate (EAU/EAM) as optimal for antioxidant-rich extracts and ACM for enzyme inhibition, positioning Rosa canina seeds as a promising source of natural bioactive compounds for functional food and therapeutic applications.

  • 
  İncele
• MakaleAçık Erişim2025/r/gumushane/13697
  Enzyme inhibitory, antioxidant, antimicrobial activities, and phenolic profiles of the methanol extract of Gelasia sericea an endemic species

  AYGÜL, İmdat; AKMEŞE, Osman; SEFALI, Abdurrahman; BALTACI, Cemalettin; ÖZ, Mehmet; FIDAN, Muhammed Said

  This study aimed to investigate, for the first time, the phytochemical composition, antioxidant, antimicrobial, and enzyme inhibitory properties of the methanolic extract of Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N.Kilian. The extract demonstrated a moderate yield (6.55 g/100 g dry plant material) and was found to be rich in phenolic acids, particularly ferulic acid (17182.97 μg g-1), followed by caffeic acid, protocatechuic acid, and p-coumaric acid. Antioxidant analyses revealed high total phenolic (388.86 mg GA eq./100 g), total flavonoid (74.03 mg QE eq./100 g), and total antioxidant capacity (503.33 mg AA eq./100 g). The extract exhibited remarkable ABTS radical scavenging activity (95.99%, IC50 = 37.59 mg ml-1) and considerable ferric-ion reducing power (974.78 mg FeSO4 eq./100 g). Antimicrobial assays indicated moderate inhibitory effects, with stronger activity against Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis) and the yeast Candida albicans, whereas Gram-negative bacteria were less susceptible. The extract’s MIC values ranged between 0.20-3.25 mg ml-1, and MLC values between 0.41-6.50 mg ml-1, which were higher than those of standard antibiotics. Enzyme inhibition assays revealed moderate activity against carbonic anhydrase II (IC50 = 0.0136 µg ml-1) and weak inhibition of cholinesterases and α-glucosidase, while α-amylase inhibition was relatively more pronounced (IC50 = 13350.00 µg ml-1). Overall, these findings highlight G. sericea as a valuable natural source of phenolic acids and antioxidants, with promising potential for applications in functional foods, nutraceuticals, and phytopharmaceuticals. However, the relatively limited antimicrobial and enzyme inhibitory activities suggest the need for further studies on bioavailability, in vivo efficacy, and compound isolation to optimize its practical applications.

  • 
  İncele
• MakaleAçık Erişim2025/r/gumushane/12498
  Screening of Chemical Profile by LC–MS/MS and Assessment of Biological Properties of Achillea sintenisii Hub. Mor. From Turkey

  Köksal, Ekrem; Altay, Ahmet; Altın, Sevgi; Alp, Cemalettin; Ulutaş, Yakup; Şimşek, Samed; Karakuş, Sinem; Akyüz, Sümeyye; Aygül, İmdat; Akşit, Hüseyin

  The aim of this study was to determine the secondary metabolites of the n-hexane, ethyl acetate, methanol, and water extracts of Achillea sintenisii Hub. Mor. and to ascertain their divergent biological properties. LC–MS/MS analysis was performed to identify the bioactive compounds. The anticancer properties of the samples were tested against human breast (MCF-7 and MDA-MB-453) and colon (HT-29) cancer cell lines by XTT assay. The anti-Alzheimer's studies were performed using Ellman's assay. The antimicrobial studies were carried out by Kirby–Bauer disc diffusion method. The antioxidative potential of the samples was evaluated on the basis of 2,2-diphenyl-1-picrylhydrazyl scavenging and ferric reducing antioxidant power assays, LC–MS/MS analysis revealed 27 phytochemicals, including high concentrations of quinic acid, gentisic acid, chlorogenic acid, luteolin, cynaroside, and caffeic acid. Ethyl acetate extract exhibited the most potent cytoxicity against MDA-MB-453 cells, with an IC50 value of 83.05 µg/mL. All extracts showed stronger inhibitory activity against acetylcholinesterase and butyrylcholinesterase compared to tacrine. The n-hexane, ethyl acetate, and methanol extracts exhibited considerable antimicrobial activity against Enterococcus faecalis, approaching the level of vancomycin. Methanol extract possessed the highest DPPH scavenging and ferric reducing antioxidant power. These findings render A, sintenisii a promising source for the pharmaceutical, cosmetic, and food industries.

  • 
  İncele
• MakaleAçık Erişim2025/r/gumushane/12385
  Chemical composition of essential oils and comparative analysis of enzyme inhibitory, antioxidant and antimicrobial activities in essential oils of endemic Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N. Kilian

  Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman; Aygül, İmdat; Öz, Mehmet

  In this study, essential oil from the aerial parts of Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N. Kilian was extracted via hydrodistillation using a Clevenger apparatus. GC-MS/FID analysis determined aldehydes (28.31%) as the dominant group, with eucalyptol (8.71%) as the main constituent. Antioxidant activities were exhibited using DPPH, ABTS, and FRAP assays, with the essential oil demonstrating relatively higher antioxidant properties. The essential oil also showed higher levels of bioactive components, including total antioxidant capacity (160.26 mg AA eq./100 mL), total phenolic content (21.57 mg GA eq./100 mL), and total flavonoid content (14.85 mg QE eq./100 mL). While G. sericea essential oil demonstrated some antioxidant activity, it was significantly lower compared to the reference standard ascorbic acid (AA). The antimicrobial results showed relatively higher antimicrobial activity compared to most bacterial and fungal strains, as evidenced by lower MIC and MBC values and larger inhibition zones. Nevertheless, chloramphenicol (CH) and nalidixic acid (NA) continually showed superior antimicrobial efficacy, with the lowest MIC and MBC values and the largest inhibition zones. The essential oil also demonstrated potent enzyme inhibitory activity, especially against CA-II, AChE, and BChE, with the lowest IC50 value observed for CA-II, highlighting its potential as a potent enzyme inhibitor. However, the essential oil exhibited comparatively greater inhibition against AChE, BChE and α-amylase but was less effective overall. These findings suggest that the G. sericea essential oil has important antioxidant potential and enzyme inhibitory activity, while the essential oil shows varying efficacy across different enzymes.

  • 
  İncele
• MakaleYalnız künye2024/r/gumushane/12764
  Assessment of Anticholinergic and Antidiabetic Properties of Some Natural and Synthetic Molecules: An In Vitro and In Silico Approach

  Çomaklı, Veysel; Aygül, İmdat; Sağlamtaş, Rüya; Kuzu, Müslüm; Demirdağ, Ramazan; Akincioğlu, Hülya; Adem, Şevki; Gülçin, İlhami

  Introduction: This study aimed to determine the in vitro and in silico effects of some natural and synthetic molecules on acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and α-glucosidase enzymes. Background: Alzheimer's disease (AD) and Type II diabetes mellitus (T2DM) are considered the most important diseases of today’s world. However, the side effects of therapeutic agents used in both diseases limit their use. Therefore, developing drugs with high therapeutic efficacy and better pharmacological profile is important. Objectives: This study sets out to determine the related enzyme inhibitors used in treating AD and T2DM, considered amongst the most important diseases of today’s world. Methods: In the current study, the in vitro and in silico effects of dienestrol, hesperetin, L-thyroxine, 3,3',5-Triiodo-L-thyronine (T3) and dobutamine molecules on AChE, BChE and α-glycosidase enzyme activities were investigated. Results: All the molecules showed an inhibitory effect on the enzymes. The IC50 and Ki values of the L-Thyroxine molecule, which showed the strongest inhibition effect for the AChE enzyme, were determined as 1.71 µM and 0.83 ± 0.195 µM, respectively. In addition, dienestrol, T3, and dobutamine molecules showed a more substantial inhibition effect than tacrine. The dobutamine molecule showed the most substantial inhibition effect for the BChE enzyme, and IC50 and Ki values were determined as 1.83 µM and 0.845 ± 0.143 µM, respectively. The IC50 and Ki values for the hesperetin molecule, which showed the strongest inhibition for the α-glycosidase enzyme, were determined as 13.57 µM and 12.33 ± 2.57 µM, respectively. Conclusion: According to the results obtained, the molecules used in the study may be considered potential inhibitor candidates for AChE, BChE and α-glycosidase.

  • 
  İncele
• MakaleYalnız künye2023/r/gumushane/20350
  Effect of White Noise and Light Exposure on Some Enzymes in Rat Testicular Tissue

  Senturk, Esra; Senturk, Murat; Ustundag, Hilal; Aygül, İmdat

  By definition, white noise (WN) is a type of sound that consists of a combination of various frequencies. Noise is an important source of environmental stress for humans and other living things and has a strong stimulating effect on light, circadian rhythm, hormonal, and neuro-behavioral levels. When faced with stressful situations, organisms produce a substantial quantity of free radicals, leading to alterations in the oxidative system. The objective of this research is to examine how environmental stressors like WN and light impact the activities of enzymes involved in testicular carbonic anhydrase (CA), catalase (CAT), and glutathione peroxidase (GPx). A total of 18 male Sprague-Dawley rats were divided into three groups. The first was the control group (not exposed to stress, kept at 12/12 light-dark cycle and 24±2°C normal ambient temperature). The second group, known as the WN 60' group, experienced 60 minutes of white noise exposure per day for 14 consecutive days at 90 dB. The third group, referred to as the WN 60' + non daylight group, underwent 60 minutes of white noise exposure at 90 dB combined with 24 hours of continuous light exposure every other day. At the conclusion of the study, the testicular tissues were homogenized and centrifuged, followed by the spectrophotometric measurement of enzyme activities. As a result, when we compared the control group with both WN and WN + light exposure; A decrease was observed for CA enzyme in both applications, and an increase was observed in CAT and GPx enzymes in both applications. As a result, when we compared the control group with both BG and BG + light exposure; A decrease was observed for CA enzyme in both applications, and an increase was observed in CAT and GPx enzymes in both applications.

  • 
  İncele
• MakaleAçık Erişim2023/r/gumushane/20257
  Spor salonuna giden bireylerin beslenme alışkanlıkları, beslenme bilgı düzeyi ve besin desteği kullanımlarının belirlenmesi

  Aygül, İmdat; Yılmaz, Hacı Ömer

  Bu araştırma, spor salonuna giden bireylerin beslenme alışkanlıkları, beslenme bilgi düzeyi ve besin desteği kullanım durumlarını belirlemek amacıyla planlanmış ve yürütülmüştür. Tanımlayıcı-kesitsel tipte olan araştırmanın verileri anket aracılığıyla elde edilmiştir. Anket formu demografik bilgiler, beslenme alışkanlıkları, Yetişkinler İçin Beslenme Bilgi Düzeyi Ölçeği ve besin desteği kullanımına yönelik sorulardan oluşmaktadır. Araştırmada 360 spor salonuna giden bireye (242 erkek, 118 kadın, ortalama yaş=22,1±5,6 yıl) ait veriler değerlendirilmiştir. Katılımcıların beslenme alışkanlıklarının önerilen düzende olmadığı, %19,4’ünün kötü düzeyde temel beslenme ve %17,5’inin kötü düzeyde besin tercih bilgisi olduğu ve %30,0’ının ise besin desteği kullandığı belirlenmiştir. Spor salonuna giden bireylerin sağlıklı beslenme alışkanlıkları kazanımı ve yüksek beslenme bilgisi edinimine bağlı olarak, daha bilinçli ve doğru şekilde besin desteği kullanım tercihinin olabileceği düşünülmektedir. Bu konuda, özellikle spor ve sporcu beslenmesi alanında uzmanlaşmış diyetisyenler bilgi edinim kaynağı olarak tercih edilebilir. Ayrıca, spor salonuna giden bireylerin beslenme konusunda sıklıkla bilgisine başvurduğu salon antrönerlerinin de besinler, besin destekleri ve sporcu beslenmesi ile ilgili bilimsel kaynakları tercih etmesi ve bireyleri daha doğru ve sağlıklı bir şekilde yönlendirmesi önerilmektedir.

  • 
  • 
  İncele