Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2026/r/gumushane/13669
  Phenolic Profiling of Laurus nobilis by LC–ESI–MS/MS: In Vitro Bioactivities and In Silico Enzyme Docking

  Bak, Funda Erşen; Canbolat Gültekin, Deniz; Akmeşe, Osman; Atalay, Abdurrahman; Turan, İbrahim; Gültekin, Ergün; Demir, Selim; Aliyazicioglu, Yüksel

  This study evaluated the branch, leaf, and fruit extracts of Laurus nobilis collected from Artvin in terms of phenolic composition, antioxidant, antimicrobial, and antidiabetic activities and additionally investigated the binding of selected phenolics to α-amylase/α-glucosidase targets through molecular docking. Using LC–ESI–MS/MS analysis, 31 phenolic compounds were quantitatively identified, with epicatechin, luteolin-7-O-glucoside, and rutin predominating particularly in the branches and leaves. Antioxidant capacity was higher in branches and leaves (total phenolic content [TPC]/FRAP/DPPH IC50: branches and leaves, respectively, 95.27–60.84 mg gallic acid equivalent [GAE]/g, 206.16–162.05 mg Trolox equivalent [TE]/g, and 46.95–113.49 µg/mL). Antimicrobial activity was observed within a minimum inhibitory concentration (MIC) range of 1.25–10 mg/mL; leaves exhibited the strongest effect against Bacillus cereus (1.25 mg/mL), whereas fruits were most effective against Candida albicans (1.25 mg/mL). In antidiabetic evaluation, fruits yielded the lowest IC50 values against α-amylase and α-glucosidase (4.31 and 8.97 mg/mL, respectively), suggesting a “composition-over-quantity” effect despite low total phenolic content. Docking results supported high binding affinities of rutin (α-glucosidase −9.3 kcal/mol; α-amylase −8.1 kcal/mol) and luteolin (−8.6 kcal/mol for both enzymes). Overall, although branches and leaves exhibited strong antioxidant profiles, fruits showed more pronounced inhibitory effects on digestive enzymes; therefore, fractionation studies and in vivo/kinetic validations are recommended.

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• MakaleYalnız künye2026/r/gumushane/13421
  LC–MS/MS Phenolic Profiling and Antioxidant, Antimicrobial, and Antidiabetic Activities of Caucasian Propolis

  Gültekin, Deniz Canbolat; Turan, İbrahim; Akmeşe, Osman; Gültekin, Ergün; Demir, Selim; Aliyazicioglu, Yüksel

  Propolis is a natural bee product rich in phenolic and flavonoid compounds and is well known for its remarkable biological properties. In this study, the phenolic compositions of propolis samples collected from various localities in the Caucasus region of Türkiye were characterized using LC–MS/MS, and their antioxidant, antidiabetic, and antibacterial activities were also evaluated. LC–MS/MS analyses identified caffeic acid, ferulic acid, and p-coumaric acid as the major phenolic acid compounds, while pinocembrin, chrysin, and galangin were determined as the major flavonoid constituents. Overall, these findings indicate that the antioxidant capacity of propolis samples obtained from different regions may be particularly associated with these key phenolic and flavonoid compounds. Consistent with this composition, total phenolic and flavonoid contents ranged from 34.72 to 120.54 mg GAE/g and 6.88 to 47.19 mg QE/g, respectively. The extracts exhibited a relatively high antioxidant capacity, with FRAP values between 60.47 and 311.87 mg TE/g and DPPH IC50 values ranging from 215.80 to 37.96 µg/mL. Regarding antidiabetic activity, the IC50 values for α-amylase and α-glucosidase inhibition were determined as 5.86–3.47 and 12.22–5.02 mg/mL, respectively, indicating a pronounced antidiabetic potential. Antimicrobial analyses revealed activity against E. faecalis, B. cereus, E. coli, K. pneumoniae, and C. albicans. Overall, this study provides a comprehensive LC–MS/MS-based phenolic profile of Caucasian propolis and demonstrates its antioxidant properties together with its antidiabetic potential.

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• MakaleAçık Erişim2025/r/gumushane/11738
  Evaluation of antibacterial, antioxidant activities and secondary metabolites profile of orchids with different dried methods

  Ozkan, Ahmet Ismail; Canbolat-Gültekin, Deniz; Soruklu, Sudenaz; Işik, Uğur; Akmeşe, Osman

  The Central Black Sea region is a very dense region in terms of orchid diversity. As a consequence of the floral studies, 32 species of salep orchids were identified in the Central Black Sea Region. The aim of this current study was to identify the secondary metabolite profiles of leaves and tubers of two orchid species (Anacamptis palustris and Ophrys apifera) with different drying methods by HPLC analysis and to determine their antibacterial and antioxidant activities. For this purpose, different drying methods were applied to the roots and leaves of these two species. The species were dried by 3 different techniques: shade, lyophilizer and oven. After the aqueous extracts were obtained, their antibacterial and antioxidant activities were tested and HPLC analysis was completed. The data obtained from aqueous extracts as a result of drying techniques were compared with the data obtained from fresh plant aqueous extracts. Antibacterial properties were tested against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 bacteria. No antibacterial activity was found in the study. Antioxidant properties were tested via DPPH and FRAP assays. As a result of DPPH free radical scavenging assay, the highest antioxidant efficiency in A. palustris was observed in leaves dried in lyophilizer (76.53%), in the shade (62.13%) and in the oven (54.12%), respectively. Nine different phenolic compounds were found in aqueous extracts of these species. Epicatechin, Gallic Acid, Rutin, Syringic Acid phenolic compounds were observed the most in HPLC analysis. The results of current study suggest that these extracts have antioxidant and phenolic contents and should be investigated in more comprehensive studies.

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• MakaleAçık Erişim2023/r/gumushane/10317
  An Investigation of the Protective Effect of Rhododendron luteum Extract on Cisplatin-Induced DNA Damage and Nephrotoxicity and Biochemical Parameters in Rats

  Turan, Ibrahim; Canbolat, Deniz; Demir, Selim; Kerimoglu, Gokcen; Colak, Fatih; Alemdar, Nihal Turkmen; Mentese, Ahmet; Aliyazicioglu, Yuksel

  The nephrotoxicity mechanisms caused by cisplatin (CP), which is used in the treatment of different types of cancer, include several processes, such as DNA damage, apoptosis, inflammation, and oxidative stress. This study investigated the protective effects of Rhododendron luteum leaf extract (RLE) in a rat model of CP -induced nephrotoxicity. Dimethyl sulfoxide extract of RLE was prepared, and its contents were clarified using liquid chromatography-mass spectrometry (LC-MS -MS). Thirty female Wistar Albino rats were divided into five different groups and sacrificed on the seventh day. Biochemical and histopathological analyses were then performed. The presence of quercitrin, quinic acid, chlorogenic acid, protocatechic acid, isoquercitrin, and quercetin was detected in the RLE. While CP significantly increased the levels of oxidative stress, inflammation, and apoptosis markers in the rat kidney tissue and blood samples, treatment with RLE significantly reversed the levels of these parameters in a dose-dependent manner. Total antioxidant status (TAS) values and glutathione (GSH) levels increased significantly in the serum samples from the treatment group compared to the CP group, while blood urea nitrogen (BUN), creatinine, oxidative stress index (OSI), tumor necrosis factor-alpha (TNF-alpha), malondialdehyde (MDA) and total oxidant status (TOS) values decreased significantly. GSH levels increased significantly in the treatment group compared to the CP group, while TNF-alpha, caspase-3 (Casp-3), 8-hydroxy-2'-deoxyguanosine (8-OHdG), OSI, TOS, MDA levels, and damage scores decreased significantly at histopathological examination. In conclusion, RLE exhibited strong protective effects against nephrotoxicity and oxidative damage caused by CP in rats.

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• MakaleYalnız künye2022/r/gumushane/13048
  An Investigation of the Antiproliferative Effect of Rhododendron luteum Extract on Cervical Cancer (HeLa) Cells via Nrf2 Signaling Pathway

  Turan, Ibrahim; Demir, Selim; Yaman, Serap Ozer; Canbolat, Deniz; Mentese, Ahmet; Aliyazicioglu, Yuksel

  The aim of the present study was to investigate the role of Rhododendron luteum extract (RLE) in the induction of Nrf2‑related oxidative stress and endoplasmic reticulum (ER) stress in human cervical cancer (HeLa) cells. The antiproliferative effect of RLE on HeLa and fibroblast cells was determined using the MTT assay. The effects of RLE on the cell cycle, apoptosis, and production of reactive oxygen species (ROS) in HeLa cells were evaluated using fluorescent probes. The mRNA expression levels of Nrf2 [and its targets glutamate-cysteine ligase catalytic subunit (GCLC), and glucose-6-phosphate dehydrogenase (G6PD)], and C/EBP homologous protein (CHOP, an ER stress marker were determined using reverse transcription‑quantitative polymerase chain reaction (RT-PCR). The results demonstrated that RLE exhibited a selective cytotoxic effect (2.9-fold) on HeLa cells compared to fibroblast cells. RLE arrested the cell cycle at the S phase, and induced apoptosis, ER stress, and ROS formation. In addition, RLE significantly suppressed the expression levels of Nrf2, GCLC and G6PD (0.65, 0.69, and 0.54-fold, respectively) and increased the expression of CHOP (4.48-fold) in HeLa cells at 72 h of treatment (p < 0.05). These results show that the antiproliferative effect of RLE occurs through the Nrf2 and ER stress pathways, and the results should now be supported by further in vivo studies.

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• MakaleAçık Erişim2021/r/gumushane/16640
  Investigation the Different Extraction Temperature Effect of Artvin Savsat Region Propolis PBS Extracts on Antioxidant Properties and Eryhrocyte Hemolysis Inhibition

  Canbolat, Deniz; Turan, Ibrahim; Küpeli, Yunus Emre; Kilinç, Sedanur; Piliç, Sevim

  Propolis, which has been used in traditional medicine for hundreds of years all over the world, is a resinous natural product with a complex content obtained by honey bees by mixing the buds of plants and pollen grains with enzymes they secrete. Propolis may have different contents due to the gathering region, plant source, bee variety and climate of the place where it is collected. Although there are many studies related to different regions, so far the propolis of the Savsat region in Artvin have not been studied yet. The aim of this study is to determine the antioxidant properties (determination of iron reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic and total flavonoid content) of propolis extracts of Artvin Savsat region prepared with phosphate buffer salt solution (PBS) prepared at different temperatures (45 and 60 degrees C) and to determine the erythrocyte hemolysis inhibition properties of the extracts. Total phenolic (11.91 +/- 0.5-24.14 +/- 1.38 mg gallic acid equivalent to g propolis) and total flavonoid (1.79 +/- 0.03-2.13 +/- 0.07 mg quercetin equivalent to g propolis) content of PBS extracts, iron power determination (12.80 +/- 0.36-27.15 +/- 0.12 mg ascorbic acid equivalent to g propolis), 2,2-diphenyl-1-picrilhydrazyl (DPPH) radical scavenging activity (%22.86 +/- 0.5-31.24 +/- 1.33) and erythrocyte hemolysis inhibition (%55.58 +/- 0.57-96.37 +/- 0.02) were determined using spectrophotometric methods. As a result of the experiments, it was determined that the extract with PBS obtained at a temperature of 60 degrees C was higher than the extract obtained at 45 degrees C, in terms of antioxidant properties and erythrocyte hemolysis inhibition ratio. Further studies are required to identify molecules that inhibit erythrocyte hemolysis in extracts and have antioxidant properties.

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• Yüksek Lisans TeziAçık Erişim2020/r/gumushane/20976
  Ratlarda cisplatinin neden olduğu DNA hasarı, nefrotoksisite ve bazı biyokimyasal parametreler üzerine Rhododendron luteum ekstraktının koruyucu etkisinin araştırılması

  Deniz Canbolat

  Kanser karmaşık bir hastalık profiline sahip dünya çapındaki en büyük halk sağlığı sorunlarından biridir. Bu hastalığa karşı birçok tedavi yöntemi kullanılmasına rağmen insan sağlığını tehdit eden nedenler arasında olma özelliğini muhafaza etmektedir. Kullanılan tedavi yöntemlerinden biri olan kemoterapide birçok ilaç kullanılmaktadır ve bu ilaçlardan biri de cisplatindir. Yapılan çalışmalarda cisplatinin kanser hücrelerinin çoğalmasını engellerken aynı zamanda sağlıklı hücreleri de etkileyerek birçok yan etkiye sebep olduğu bildirilmiştir. Cisplatin kullanımının yan etkileri özellikle reaktif oksijen türlerine (ROS) atfedilmekte olup bu yan etkilere karşı koruyucu yeni yaklaşımlar geliştirilmektedir. Bu amaç doğrultusunda antioksidan maddeler ile cisplatinin birlikte kullanımının olduğu birçok çalışma bulunmaktadır. Bu çalışmada, ratlarda cisplatine bağlı olarak oluşan DNA hasarı, oksidatif stres artışı, apoptoz, inflamasyon ve nefrotoksisite gibi hasarlara karşı Rhododendron luteum yaprak ekstraktının koruyucu etkisinin incelenmesi amaçlanmıştır. Cisplatin kaynaklı nefrotoksisite modeli Wistar Albino türü dişi sıçanlarla oluşturulmuş olup sıçanlar rastgele 5 farklı gruba (n=6; kontrol grubu, dimetil sülfoksit (DMSO) grubu, cisplatin grubu, 12,5 mg/kg Rhododendron luteum tedavi grubu ve 25 mg/kg Rhododendron luteum tedavi grubu) ayrıldı. Kontrol grubuna 6 gün 0,5 ml'lik serum fizyolojik, çözücü grubuna 6 gün 0,5 ml'lik DMSO, cisplatin grubuna 1. gün tek doz 7,5 mg/kg'lık cisplatin ve 5 gün 0,5 ml'lik serum fizyolojik, tedavi gruplarına ise 1. gün 7,5 mg/kg'lık cisplatin ve 5 gün 0,5 ml'lik Rhododendron luteum ekstraktı (12,5 mg/kg ve 25 mg/kg) intraperitoneal olarak verildi ve ratlar 7. günde sakrifiye edildi. Böbrek dokusunda ve kanda; malondialdehit (MDA), toplam antioksidan kapasite (TAS), toplam oksidan kapasite (TOS), oksidatif stres indeksi (OSİ), süperoksit dismutaz (SOD), Kaspaz-3, tümör nekroz faktör-alfa (TNF-α), glutatyon (GSH), 8-hidroksi-2'-deoksiguanozin (8-OHdG) düzeyleri ölçüldü ve böbrek dokusunun histopatolojik değerlendirmesi yapıldı. Böbrek doku örneklerinde tedavi grubunun cisplatin grubuna göre MDA, TOS, OSİ, Kaspaz-3, TNF-α, 8-OHdG anlamlı düzeyde azalırken (p<0.05), GSH seviyelerinin anlamlı düzeyde arttığı gözlemlendi (p<0.05). Serum örneklerinde MDA, TOS, OSİ, TNF-α seviyeleri anlamlı düzeyde azalırken (p<0.05) GSH ve TAS seviyeleri anlamlı düzeyde arttığı gözlemlendi (p<0.05). Histopatolojik incelemede ise tedavi gruplarının cisplatin grubuna kıyasla hasar skorunu azalttığı gözlemlendi. Elde edilen sonuçlar doğrultusunda cisplatinin ratlarda nefrotoksisiteye neden olduğu ve oluşan bu toksisite üzerine Rhododendron luteum'un koruyucu etkilerinin olduğu gözlemlenmiştir.

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