Sürdürülebilir Kalkınma Amaçları

• 1

Yazarlar

• Akkoyunlu, Yasemin(1)
• Beris, Fatih Saban(1)
• Doymaz, Mehmet Ziya(1)
• Düzgün, Azer Ozad(1)
• Iraz, Meryem(1)
• Karaoglu, Hakan(1)
• Peleg, Anton Y.(1)
• Sandalli, Cemal(1)
• Saral, Aysegul(1)
• Çiçek, Aysegul Copur(1)
• Özgümüs, Osman Birol(1)

• MakaleAçık Erişim2015Scopus SJRQ1/r/gumushane/10651
  Distribution of β-Lactamase Genes Among Carbapenem-Resistant Klebsiella pneumoniae Strains Isolated From Patients in Turkey

  Iraz, Meryem; Düzgün, Azer Ozad; Sandalli, Cemal; Doymaz, Mehmet Ziya; Akkoyunlu, Yasemin; Saral, Aysegul; Peleg, Anton Y.; Özgümüs, Osman Birol; Beris, Fatih Saban; Karaoglu, Hakan

  Background: The emergence of carbapenem-resistant Klebsiella pneumoniae poses a serious problem to antibiotic management. We investigated the beta-lactamases in a group of carbapenem-resistant K. pneumoniae clinical isolates from Turkey. Methods: Thirty-seven strains of K. pneumoniae isolated from various clinical specimens were analyzed by antimicrobial susceptibility testing, PCR for the detection of p-lactamase genes, DNA sequencing, and repetitive extragenic palindronic (REP)- FOR analysis. Results: All 37 isolates were resistant to ampicillin, ampicillin/sulbactam, piperacillin, piperacillin/tazobactam, ceftazidime, cefoperazone/sulbactam, cefepime, imipenem, and meropenem. The lowest resistance rates were observed for colistin (2.7%), tigecycline (11%), and amikacin (19%). According to FOR and sequencing results, 98% (36/37) of strains carried at least one carbapenemase gene, with 32 (86%) carrying OXA-48 and 7 (19%) carrying NDM-1. No other carbapenemase genes were identified. All strains carried a CTX-M-2-like beta-lactamase, and some carried SHV- (97%), TEM- (9%), and CTX-M-1-like (62%) beta-lactamases. Sequence analysis of bla(TEM) genes identified a bla(TEM-166) with an amino acid change at position 53 (Arg53Gly) from bla(TEM-1b), the first report of a mutation in this region. REP-FOR analysis revealed that there were seven different clonal groups, and temporo-spatial links were identified within these groups. Conclusions: Combinations of beta-lactamases were found in all strains, with the most common being OXA-48, SHV, TEM, and CTX-M-type (76% of strains). We have reported, for the first time, a high prevalence of the NDM-1 (19%) carbapenemase in carbapenem-resistant K. pneumoniae from Turkey. These enzymes often co-exist with other beta-lactamases, such as TEM, SHV, and CTX-M beta-lactamases.

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  AtıfScopus55·WoS46·OpenAlex56

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