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- MakaleAçık Erişim2025Investigation of virulence factor genes and biofilm formation of antibiotic resistant clinical E.coli isolates
Mumin, Yahye Mohamud; Yüksel, Gamze; Özad Düzgün, Azer
Purpose: The aim of this study is to investigate the antibiotic sensitivity, presence of virulence genes and biofilm formation capacity of 90 clinical E. coli isolates. Methods: The presence of virulence genes in E. coli isolates were investigated by PCR. Ninety clinical isolates of E. coli were subjected to biofilm quantitative analysis using the semi-quantitative crystal violet staining method. Results: it was observed that the isolates were resistant to quinolone, cephalosporin, aminoglycoside, carbapenem and penicillin group antibiotics. The presence of virulence factor genes were observed in a total of 86/90 E. coli. The highest rate of fim (92.2 %) virulence factor gene was detected in the strains. Afa, pap, cnf, sfa, hly were detected in 30 %, 13 %, 13 %, 3.3 %, 2.2 % respectively. Also, 13 different virulence factor gene patterns were determined in 90 E. coli isolates. Of the 90 E. coli isolates whose biofilm-forming capacities were evaluated, 42 were found to have biofilm-forming capacity. Of these 26 (28.8 %) the weak, 12 (13.3 %) moderate and 4 (4.4 %) strong biofilm-forming. Also, statistical analysis was performed to investigate the relationship between virulence factor genes and biofilm formation, and none of the 7 genes analyzed showed a significant relationship with biofilm formation. Conclusion: since pathogenic E. coli is an important public health problem, investigating antibiotic resistance, virulence factor genes and biofilm formation in bacterial pathogens is important for better treatment options.
- MakaleAçık Erişim2023Detection of virulence factor genes, antibiotic resistance genes and biofilm formation in clinical Gram-negative bacteria and first report from Türkiye of K.oxytoca carrying both bla OXA-23 and bla OXA-51 genes
Özad Düzgün, Azer; Yüksel, Gamze
The Gram-negative bacteria were isolated from various samples and antibiotic susceptibility testing were performed. The strains were screened by PCR for class 1 integron, virulence and antibiotic resistance genes. blaOXA-23 and blaOXA-51 genes positive samples were cloned into the pGEM-T vector and the plasmids were sent for DNA sequence analysis. The isolates were showed resistance to quinolone, cephalosporin, aminoglycoside, carbapenem and penicillin group antibiotics. Virulence factor genes were identified in 121 strain. The most common virulence gene was determined to be aer (29.78%), followed by sfa (28.19%), afa (27.12%), cnf (21.27%), pap (12.76%) and hly (12.76%). In addition, 34 different virulence patterns were observed. Plasmid-mediated quinolone resistance genes were detected 15 of67 quinolone-resistant isolates. Among carbapenem resistant strains, blaOXA-23 gene was detected in 16 and blaOXA-51 gene in 19 isolates. The strains producing OXA-23 and OXA-51 were Klebsiella oxytoca, Acinetobacter baumannii, E.coli and P.mirabilis. In this study, clinical isolate K. oxytoca carrying both blaOXA-23 and blaOXA-51 genes were identified first time from Türkiye. A high predominance (88.82%) of class 1 integron was detected. In addition, determined that 46.27% of the isolates had the ability to form biofilms. In conclusion since the limited treatment options of antibiotic-resistant Gram-negative pathogens that cause serious infections are an important problem in terms of public health it is important to investigate antibiotic resistance, virulence factor genes and biofilm formation in these pathogens.