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- MakaleYalnız künye2023Scopus SJRQ1Increased heat tolerance and transcriptome analysis of Salmonella enterica Enteritidis PT 30 heat-shocked at 42 ℃
Qiu, Yan; Ozturk, Samet; Cui, Xinyao; Qin, Wen; Wu, Qingping; Liu, Shuxiang
In this study, we compared the heat tolerance parameter (D65℃) values of Salmonella enterica serovar Enteritidis PT 30 (S. Enteritidis ) heat adapted at different degrees (at 42 ℃ for 20–180 min) and cultivated using two methods. The treated group with the highest D65℃ value (LP-42 ℃-60 min) and the untreated groups (Control-TSB and Control-TSA) were subjected to transcriptome analysis. Heat-adaptation increased the D65℃ values of S. Enteritidis by 24.5–60.8%. The D65℃ values of the LP-42 ℃-60 min group (1.85 ± 0.13 min, 7.7% higher) was comparable to that of the Control-TSA. A total of 483 up- and 443 downregulated genes of S. enteritidis were identified in the LP-42 ℃-60 min group (log2fold change > 1, adjusted p-value < 0.05). Among these genes, 5 co-expressed and 15 differentially expressed genes in the LP-42 ℃-60 min and Control-TSA grops possibly contributed to the high D65℃ values of S. Enteritidis. The Rpo regulon was involved in the heat adaptation of S. Enteritidis, as evidenced by the significant upregulation of rpoS, rpoN, and rpoE. KEGG enrichment pathways, such as biosynthesis of secondary metabolites, tricarboxylic acid, and ribosomes were identified and mapped to reveal the molecular mechanisms of S. enteritidis during heat adaptation. This study quantified the enhanced heat tolerance of S. Enteritidis heat adapted at different degrees of heat-adaptation. The results of this study may serve as a basis for elucidating the molecular mechanisms underlying the enhanced heat tolerance at the transcriptome level.
AtıfScopus9WoS9OpenAlex10
İncele - MakaleYalnız künye2017Scopus SJRQ3Investigation of the effects of some modification process to the mechanical performance and deformation of the woodwork
Onduran, Aytekin; Yasar, S. Sadiye; Altunok, Mustafa
In this study, wood modification (thermo-processing and impregnation) and outdoor conditions of storage (natural aging) the effects on the mechanical performance of industrial woodwork was investigated. For this purpose, naturally grown in Turkey and the commercial value of high Scots pine (Pinus sylvestris L.) and chestnut (Castanea sativa Mill) wood was used. Experimental examples are specifically woodwork has been applied in actual size. Considering the previously conducted research literature only diagonal L-type samples used, not on the actual size samples of woodwork. In addition, there is no study that the combination of the heat-treatment and impregnation. This reveals the original values of this study. For preparing of samples is used the double tenon-mortise corner joint from constructions method and as glue polyurethane based Desmodur VTKA and Polyvinyl acetate based two-component D4. Diagonal woodwork test samples prepared by Thermo S class (185°C temperature with a thermo-vapor process protections) applying. After application of the heat treatment, the test samples impregnated with a solution of 95 % natural pine cone resin and %5 pine tannin by dip method (2 hours). Then, the untreated samples (control), only heat-treated samples and heat treatment + impregnated samples kept for 1 year with outdoor conditions (aging). At the end of the aging, the samples tested according to the principles stated in TS 2472 and TS 7251 EN 107 standard. As result, the performance of woodwork decreased after aging in untreated and heat treatment pieces, the other side after aging - heat treatment, impregnation materials increased the values. The deformation value of woodwork increased after aging most untreated materials, Heat treatment and heat treatment-impregnated reduced aging effectiveness.
AtıfScopus3WoS3İncele